|Institutional Source||Beutler Lab|
|Gene Name||vasoactive intestinal polypeptide|
|Synonyms||PHI, peptide histidine isoleucine|
|Is this an essential gene?||Probably non essential (E-score: 0.135)|
|Stock #||R0267 (G1)|
|Chromosomal Location||5639218-5647617 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 5644004 bp|
|Amino Acid Change||Aspartic acid to Valine at position 119 (D119V)|
|Ref Sequence||ENSEMBL: ENSMUSP00000019906 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000019906]|
|Predicted Effect||possibly damaging
AA Change: D119V
PolyPhen 2 Score 0.667 (Sensitivity: 0.86; Specificity: 0.91)
AA Change: D119V
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.1045|
|Coding Region Coverage||
|Validation Efficiency||97% (64/66)|
FUNCTION: This gene encodes a neuropeptide of the glucagon/secretin superfamily with potent bronchodilator, immunomodulator and anti-inflammatory properties. The encoded protein is proteolytically processed to generate two structurally similar neuropeptides - vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI). In the digestive tract, VIP stimulates relaxation of enteric smooth muscle, secretion of water and electrolytes, release of insulin and glucagon, and inhibition of gastric acid secretion. In the cardiovascular system, VIP causes coronary vasodilation and stimulates contractility in the heart. Mice lacking VIP exhibit airway hyperresponsiveness and airway inflammation. Male mice lacking VIP exhibit moderate pulmonary arterial hypertension resulting in increased mortality. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
PHENOTYPE: Homozygous null mutants display abnormal circadian rhythyms with a shorter period, abnormal phase, and in 1/4 arrhythmic circadian persistence. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Vip||
(F):5'- TCACCAGCGATTACAGCAGACTTC -3'
(R):5'- GCCTGACAGCAGCCAAAATTGAG -3'
(F):5'- gttttttgtttgtttgcttgcttg -3'
(R):5'- acagcagccaaaattgagaaTGAG -3'