Incidental Mutation 'R4613:Map3k11'
ID 351016
Institutional Source Beutler Lab
Gene Symbol Map3k11
Ensembl Gene ENSMUSG00000004054
Gene Name mitogen-activated protein kinase kinase kinase 11
Synonyms Mlk3, 2610017K16Rik
MMRRC Submission 041824-MU
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.340) question?
Stock # R4613 (G1)
Quality Score 225
Status Not validated
Chromosome 19
Chromosomal Location 5738770-5752893 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to T at 5747499 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamine to Histidine at position 578 (Q578H)
Ref Sequence ENSEMBL: ENSMUSP00000004156 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000004156]
AlphaFold Q80XI6
Predicted Effect probably damaging
Transcript: ENSMUST00000004156
AA Change: Q578H

PolyPhen 2 Score 0.982 (Sensitivity: 0.75; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000004156
Gene: ENSMUSG00000004054
AA Change: Q578H

DomainStartEndE-ValueType
low complexity region 11 36 N/A INTRINSIC
SH3 45 105 6.79e-19 SMART
TyrKc 118 377 6.83e-81 SMART
coiled coil region 398 444 N/A INTRINSIC
low complexity region 467 476 N/A INTRINSIC
low complexity region 593 610 N/A INTRINSIC
low complexity region 614 632 N/A INTRINSIC
low complexity region 676 697 N/A INTRINSIC
low complexity region 759 778 N/A INTRINSIC
low complexity region 786 805 N/A INTRINSIC
low complexity region 809 820 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.5%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a targeted or spontaneous mutation display a pattern of dark red stripes of thin dorsal epithelium during pre-wean development, and necrotic dental pulp has been reported for the spontaneous mutant. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 82 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9830107B12Rik A T 17: 48,439,167 (GRCm39) L130I probably benign Het
Abca9 A G 11: 110,035,610 (GRCm39) V670A probably benign Het
Adad1 A G 3: 37,146,182 (GRCm39) N517D probably damaging Het
Ankle2 T C 5: 110,379,245 (GRCm39) L48P probably benign Het
Aoc3 A T 11: 101,228,485 (GRCm39) probably benign Het
Areg A G 5: 91,291,363 (GRCm39) K102R probably benign Het
Bmp1 T C 14: 70,745,963 (GRCm39) T167A probably damaging Het
C4b C T 17: 34,953,525 (GRCm39) G986D probably benign Het
Caml G T 13: 55,772,955 (GRCm39) G200C probably damaging Het
Ccdc40 A C 11: 119,122,358 (GRCm39) R53S probably benign Het
Cdh22 T A 2: 164,985,576 (GRCm39) I337L probably benign Het
Col12a1 A T 9: 79,554,883 (GRCm39) V2065D probably benign Het
Copg2 A G 6: 30,788,531 (GRCm39) S591P probably benign Het
Cyp2d34 G A 15: 82,500,526 (GRCm39) P438S probably damaging Het
Dchs1 T C 7: 105,421,931 (GRCm39) D163G probably damaging Het
Depdc1b T C 13: 108,500,177 (GRCm39) V230A probably damaging Het
Depdc5 T A 5: 33,132,790 (GRCm39) L1300H probably damaging Het
Dnah10 G T 5: 124,839,933 (GRCm39) probably null Het
Dsc2 A T 18: 20,174,876 (GRCm39) D466E probably damaging Het
Dthd1 A G 5: 62,984,411 (GRCm39) D372G probably damaging Het
Eeig2 A T 3: 108,934,571 (GRCm39) F23I probably benign Het
Eogt G T 6: 97,111,265 (GRCm39) Q199K probably benign Het
Epha6 C A 16: 59,486,960 (GRCm39) R1029L possibly damaging Het
Eppin C A 2: 164,431,243 (GRCm39) E128* probably null Het
Fgf20 T C 8: 40,739,652 (GRCm39) R33G probably benign Het
Fgg A G 3: 82,917,397 (GRCm39) N142S probably damaging Het
Fhip2a C A 19: 57,359,619 (GRCm39) P53Q probably damaging Het
Gba1 C T 3: 89,115,951 (GRCm39) probably null Het
Gli2 A G 1: 118,765,241 (GRCm39) V970A probably damaging Het
Gramd1b A T 9: 40,219,289 (GRCm39) V508D probably damaging Het
Gucy2c T A 6: 136,685,319 (GRCm39) D898V probably damaging Het
Kcnj15 T C 16: 95,096,653 (GRCm39) Y92H probably damaging Het
L3mbtl3 A T 10: 26,158,693 (GRCm39) S652T unknown Het
Ldb2 G T 5: 44,633,893 (GRCm39) Q326K probably benign Het
Lipi C A 16: 75,357,689 (GRCm39) R292L probably benign Het
Lrrc36 G A 8: 106,176,246 (GRCm39) V207I possibly damaging Het
Lyplal1 C T 1: 185,820,949 (GRCm39) G166D probably benign Het
Map1b A T 13: 99,566,810 (GRCm39) Y1970* probably null Het
Map2 A G 1: 66,464,628 (GRCm39) N287D probably damaging Het
Map4k4 G A 1: 40,056,351 (GRCm39) S1012N probably benign Het
Mapk13 A T 17: 28,988,426 (GRCm39) N15Y probably damaging Het
Mapk15 G A 15: 75,867,759 (GRCm39) A125T probably damaging Het
Mrgprb1 C A 7: 48,097,456 (GRCm39) R152L possibly damaging Het
Mtrex C A 13: 113,058,273 (GRCm39) E53* probably null Het
Muc5ac T G 7: 141,344,840 (GRCm39) Y104D possibly damaging Het
Myo1h A G 5: 114,486,440 (GRCm39) N566S possibly damaging Het
Myo1h C A 5: 114,489,737 (GRCm39) H647Q probably benign Het
Neo1 A G 9: 58,796,324 (GRCm39) I1201T possibly damaging Het
Nlgn1 T C 3: 25,490,186 (GRCm39) T514A probably benign Het
Or10al4 A G 17: 38,037,587 (GRCm39) Y224C probably damaging Het
Or12j4 A T 7: 140,046,981 (GRCm39) Y289F probably damaging Het
Or7a42 A G 10: 78,791,899 (GRCm39) N287D probably damaging Het
Or8d4 T A 9: 40,039,018 (GRCm39) K80* probably null Het
Orc2 A T 1: 58,539,468 (GRCm39) L57* probably null Het
Otoa G A 7: 120,744,791 (GRCm39) V850M probably damaging Het
Pcnx3 T C 19: 5,717,247 (GRCm39) T1579A possibly damaging Het
Pde5a A T 3: 122,616,742 (GRCm39) Y564F probably damaging Het
Pdxk A C 10: 78,283,753 (GRCm39) I147S probably damaging Het
Pfdn5 A G 15: 102,237,187 (GRCm39) D108G probably benign Het
Pink1 T C 4: 138,044,621 (GRCm39) D342G probably damaging Het
Prkacb A T 3: 146,443,753 (GRCm39) V336E probably damaging Het
Ptpro C A 6: 137,393,834 (GRCm39) S13* probably null Het
Rfng A G 11: 120,673,476 (GRCm39) L215P probably damaging Het
Rpn2 T C 2: 157,144,345 (GRCm39) F336L possibly damaging Het
Sacs A G 14: 61,449,246 (GRCm39) probably null Het
Sirpb1a T A 3: 15,482,097 (GRCm39) Y77F probably benign Het
Slc30a8 A G 15: 52,196,971 (GRCm39) D294G probably benign Het
Sox13 T C 1: 133,316,672 (GRCm39) I212V probably benign Het
Srebf2 T A 15: 82,069,549 (GRCm39) I657N possibly damaging Het
Srsf6 C A 2: 162,775,629 (GRCm39) T146K probably benign Het
Strn4 T C 7: 16,558,088 (GRCm39) V162A possibly damaging Het
Sulf2 T C 2: 165,974,525 (GRCm39) D53G probably damaging Het
Tbc1d8 T A 1: 39,411,789 (GRCm39) I1016F probably damaging Het
Tfrc G T 16: 32,437,475 (GRCm39) A278S probably damaging Het
Tnrc6a T G 7: 122,783,512 (GRCm39) probably null Het
Vmn1r83 T C 7: 12,055,695 (GRCm39) I121V probably benign Het
Vps13d T C 4: 144,858,225 (GRCm39) S2200G possibly damaging Het
Washc2 T A 6: 116,206,230 (GRCm39) D397E probably damaging Het
Wipf3 T C 6: 54,462,540 (GRCm39) L250P probably damaging Het
Xirp1 A G 9: 119,848,748 (GRCm39) F45S probably damaging Het
Xpo5 A G 17: 46,547,889 (GRCm39) T910A probably benign Het
Zfp235 T C 7: 23,841,101 (GRCm39) Y507H probably damaging Het
Other mutations in Map3k11
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02017:Map3k11 APN 19 5,747,651 (GRCm39) missense possibly damaging 0.91
IGL02581:Map3k11 APN 19 5,750,834 (GRCm39) missense probably benign
pow UTSW 19 5,750,622 (GRCm39) missense probably benign 0.03
yow UTSW 19 5,747,429 (GRCm39) missense probably benign 0.34
R0130:Map3k11 UTSW 19 5,740,843 (GRCm39) missense probably damaging 1.00
R0193:Map3k11 UTSW 19 5,745,874 (GRCm39) missense probably damaging 0.99
R1621:Map3k11 UTSW 19 5,740,834 (GRCm39) missense probably damaging 1.00
R1736:Map3k11 UTSW 19 5,747,429 (GRCm39) missense probably benign 0.34
R1791:Map3k11 UTSW 19 5,745,600 (GRCm39) nonsense probably null
R3500:Map3k11 UTSW 19 5,740,275 (GRCm39) start codon destroyed probably benign 0.08
R3836:Map3k11 UTSW 19 5,740,831 (GRCm39) missense possibly damaging 0.71
R3892:Map3k11 UTSW 19 5,752,311 (GRCm39) missense probably benign 0.13
R4303:Map3k11 UTSW 19 5,740,852 (GRCm39) missense probably damaging 1.00
R4513:Map3k11 UTSW 19 5,752,238 (GRCm39) missense probably damaging 0.97
R4613:Map3k11 UTSW 19 5,747,498 (GRCm39) missense probably benign 0.18
R4631:Map3k11 UTSW 19 5,740,941 (GRCm39) missense probably benign 0.30
R4780:Map3k11 UTSW 19 5,740,966 (GRCm39) missense probably damaging 0.99
R5213:Map3k11 UTSW 19 5,740,669 (GRCm39) missense probably damaging 0.99
R5266:Map3k11 UTSW 19 5,750,622 (GRCm39) missense probably benign 0.03
R5372:Map3k11 UTSW 19 5,740,990 (GRCm39) missense probably damaging 1.00
R5736:Map3k11 UTSW 19 5,746,739 (GRCm39) missense probably damaging 1.00
R5899:Map3k11 UTSW 19 5,745,937 (GRCm39) critical splice donor site probably null
R6052:Map3k11 UTSW 19 5,747,430 (GRCm39) missense probably benign 0.01
R6388:Map3k11 UTSW 19 5,740,279 (GRCm39) missense probably damaging 0.96
R6623:Map3k11 UTSW 19 5,745,631 (GRCm39) missense probably damaging 0.97
R6975:Map3k11 UTSW 19 5,740,755 (GRCm39) missense possibly damaging 0.91
R7309:Map3k11 UTSW 19 5,740,486 (GRCm39) missense probably damaging 0.99
R7771:Map3k11 UTSW 19 5,740,636 (GRCm39) missense probably damaging 0.99
R7815:Map3k11 UTSW 19 5,745,667 (GRCm39) missense possibly damaging 0.89
R8988:Map3k11 UTSW 19 5,752,166 (GRCm39) missense probably damaging 1.00
R9130:Map3k11 UTSW 19 5,746,038 (GRCm39) missense possibly damaging 0.69
R9502:Map3k11 UTSW 19 5,740,624 (GRCm39) missense probably damaging 1.00
X0025:Map3k11 UTSW 19 5,746,223 (GRCm39) frame shift probably null
Predicted Primers PCR Primer
(F):5'- ACCCACCCATCCTAGCTGTTAG -3'
(R):5'- TCAGCAAGATCCATGAGCTC -3'

Sequencing Primer
(F):5'- CTAGCTGTTAGGATGTAGGATAAGG -3'
(R):5'- AGATCCATGAGCTCCGTACTC -3'
Posted On 2015-10-08