Mutagenetix > Incidental Mutation

Incidental Mutation 'R4616:Vmn2r59'

351042

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r59

Ensembl Gene

ENSMUSG00000092032

Gene Name

vomeronasal 2, receptor 59

Synonyms

EG628444

MMRRC Submission

041827-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R4616 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42011792-42058981 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 42012438 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 651 (I651T)

Ref Sequence

ENSEMBL: ENSMUSP00000131856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168489]

AlphaFold

E9PUT5

Predicted Effect

probably benign
Transcript: ENSMUST00000168489
AA Change: I651T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000131856
Gene: ENSMUSG00000092032
AA Change: I651T

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.8e-44	PFAM
Pfam:NCD3G	514	567	4.3e-23	PFAM
Pfam:7tm_3	600	835	5.4e-53	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

100% (64/64)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010111I01Rik	G	A	13: 63,298,751	E123K	probably damaging	Het
4931417E11Rik	A	G	6: 73,469,269	V99A	probably benign	Het
Acod1	C	T	14: 103,055,345	T435M	probably benign	Het
Adgrf5	T	C	17: 43,452,440	F1078L	probably benign	Het
Adh6a	A	T	3: 138,324,947	N110I	probably damaging	Het
Aldh16a1	T	C	7: 45,148,788		probably benign	Het
Arhgef17	A	G	7: 100,882,485	F1302S	probably damaging	Het
Bcl2a1a	C	T	9: 88,957,453	R135W	probably damaging	Het
Bpifa6	T	C	2: 153,982,988	S28P	possibly damaging	Het
C9	A	G	15: 6,491,463	D51G	probably damaging	Het
Cfb	T	A	17: 34,859,068	H962L	probably benign	Het
Chn2	G	A	6: 54,290,403	M292I	probably damaging	Het
Clec16a	T	A	16: 10,644,883		probably null	Het
Cyp1a1	T	C	9: 57,701,756	S307P	probably benign	Het
Dsg3	T	C	18: 20,531,559	V538A	probably benign	Het
Erbb3	G	A	10: 128,572,770	Q815*	probably null	Het
Fam90a1a	C	A	8: 21,963,846	Q406K	possibly damaging	Het
Frmd3	T	C	4: 74,187,872	V585A	probably benign	Het
Gm7102	C	T	19: 61,175,926	G24R	unknown	Het
Gm8214	T	C	1: 183,681,897		noncoding transcript	Het
Gpld1	G	A	13: 24,984,816	G771D	probably damaging	Het
Gpr20	T	C	15: 73,695,736	N268S	probably benign	Het
Gria2	A	T	3: 80,706,897	I612N	probably damaging	Het
Ifit1bl1	T	C	19: 34,594,610	E149G	probably damaging	Het
Ighv1-82	T	C	12: 115,952,660	T77A	probably benign	Het
Ighv2-9	G	T	12: 113,879,219	T76K	probably damaging	Het
Igkv6-13	A	T	6: 70,458,035	M1K	probably null	Het
Igkv8-21	A	T	6: 70,315,157	S34T	probably benign	Het
Itpr1	A	G	6: 108,481,223	N1985D	probably damaging	Het
Lama3	T	C	18: 12,504,397		probably null	Het
Lamc2	T	C	1: 153,166,169	Y73C	probably damaging	Het
Maff	A	G	15: 79,357,698	D105G	probably damaging	Het
Mep1a	C	T	17: 43,486,241	V312M	possibly damaging	Het
Mfap4	C	A	11: 61,485,509		probably benign	Het
Mrgbp	A	T	2: 180,585,314		silent	Het
Mtmr4	T	C	11: 87,610,935	L548S	probably damaging	Het
Myo7b	T	C	18: 32,003,487		probably null	Het
Myo9a	T	C	9: 59,821,649	I596T	probably damaging	Het
Olfr38	G	T	6: 42,762,418	R122L	probably benign	Het
Olfr594	C	T	7: 103,220,422	R235*	probably null	Het
Pcsk5	T	G	19: 17,560,750	Q904H	probably benign	Het
Pdzrn3	G	T	6: 101,152,009	H565Q	probably damaging	Het
Phkg2	C	T	7: 127,577,620	R61W	probably damaging	Het
Pkd2l1	C	A	19: 44,154,134	A490S	probably damaging	Het
Pomgnt1	T	A	4: 116,154,890	I337N	probably damaging	Het
Psmd3	T	C	11: 98,682,926	V66A	probably benign	Het
Ptger3	T	C	3: 157,567,294	S93P	probably damaging	Het
Rbm27	T	A	18: 42,301,775	D301E	probably damaging	Het
Rdh16	A	T	10: 127,801,513		probably null	Het
Slc35a5	A	T	16: 45,144,292	F193I	probably benign	Het
Slc4a4	A	G	5: 89,038,561	K167R	probably damaging	Het
Sort1	A	T	3: 108,355,541	T772S	possibly damaging	Het
Sptbn5	T	A	2: 120,048,757		noncoding transcript	Het
Stard5	G	T	7: 83,633,281		probably benign	Het
Tbc1d22a	A	G	15: 86,235,685	T61A	probably damaging	Het
Tox2	T	C	2: 163,320,647	L479P	probably damaging	Het
Usp53	A	T	3: 122,959,120	M80K	probably damaging	Het
Vmn1r209	A	T	13: 22,805,965	L185Q	probably damaging	Het
Vsig1	G	T	X: 140,926,386	A95S	probably benign	Het
Zfhx4	T	C	3: 5,413,067	S3556P	possibly damaging	Het

Other mutations in Vmn2r59

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Vmn2r59	APN	7	42012064	missense	possibly damaging	0.91
IGL01432:Vmn2r59	APN	7	42012559	missense	possibly damaging	0.82
IGL02119:Vmn2r59	APN	7	42046169	missense	probably benign	0.36
IGL02216:Vmn2r59	APN	7	42012393	missense	probably damaging	1.00
IGL02327:Vmn2r59	APN	7	42012231	missense	probably benign
IGL03346:Vmn2r59	APN	7	42043829	missense	probably benign	0.00
IGL03411:Vmn2r59	APN	7	42058916	missense	probably benign	0.43
IGL03412:Vmn2r59	APN	7	42012438	missense	probably benign
PIT4366001:Vmn2r59	UTSW	7	42045781	missense	possibly damaging	0.91
R0068:Vmn2r59	UTSW	7	42046301	missense	probably damaging	0.99
R0094:Vmn2r59	UTSW	7	42012298	missense	probably benign	0.07
R0179:Vmn2r59	UTSW	7	42047008	nonsense	probably null
R0370:Vmn2r59	UTSW	7	42012726	missense	probably benign	0.23
R0412:Vmn2r59	UTSW	7	42046492	splice site	probably benign
R0465:Vmn2r59	UTSW	7	42046908	missense	probably benign
R0487:Vmn2r59	UTSW	7	42047104	nonsense	probably null
R0576:Vmn2r59	UTSW	7	42047105	missense	probably benign	0.01
R0632:Vmn2r59	UTSW	7	42058884	missense	probably damaging	1.00
R1356:Vmn2r59	UTSW	7	42011794	makesense	probably null
R1387:Vmn2r59	UTSW	7	42046097	missense	probably damaging	1.00
R1388:Vmn2r59	UTSW	7	42045709	missense	probably benign	0.01
R1435:Vmn2r59	UTSW	7	42046205	missense	possibly damaging	0.50
R1750:Vmn2r59	UTSW	7	42045827	missense	possibly damaging	0.50
R2020:Vmn2r59	UTSW	7	42043779	missense	probably damaging	1.00
R2249:Vmn2r59	UTSW	7	42058902	missense	probably benign	0.00
R2256:Vmn2r59	UTSW	7	42012245	nonsense	probably null
R2257:Vmn2r59	UTSW	7	42012245	nonsense	probably null
R2441:Vmn2r59	UTSW	7	42046146	missense	probably benign	0.00
R2511:Vmn2r59	UTSW	7	42043766	missense	probably damaging	1.00
R2860:Vmn2r59	UTSW	7	42047003	missense	possibly damaging	0.79
R2861:Vmn2r59	UTSW	7	42047003	missense	possibly damaging	0.79
R3690:Vmn2r59	UTSW	7	42011946	missense	possibly damaging	0.77
R3912:Vmn2r59	UTSW	7	42046320	missense	probably benign	0.00
R4167:Vmn2r59	UTSW	7	42021308	intron	probably benign
R4357:Vmn2r59	UTSW	7	42012220	missense	probably damaging	1.00
R4445:Vmn2r59	UTSW	7	42042450	missense	probably damaging	1.00
R4542:Vmn2r59	UTSW	7	42046073	missense	possibly damaging	0.93
R4587:Vmn2r59	UTSW	7	42046224	missense	probably benign	0.00
R4653:Vmn2r59	UTSW	7	42043804	missense	probably benign	0.19
R4703:Vmn2r59	UTSW	7	42012262	missense	probably benign	0.01
R4895:Vmn2r59	UTSW	7	42045794	missense	probably damaging	0.98
R4910:Vmn2r59	UTSW	7	42043653	missense	probably benign
R5045:Vmn2r59	UTSW	7	42046072	missense	possibly damaging	0.93
R5105:Vmn2r59	UTSW	7	42047105	missense	probably benign	0.01
R5153:Vmn2r59	UTSW	7	42042410	critical splice donor site	probably null
R5566:Vmn2r59	UTSW	7	42046823	missense	possibly damaging	0.92
R5586:Vmn2r59	UTSW	7	42045681	missense	probably benign	0.12
R5606:Vmn2r59	UTSW	7	42045894	missense	probably benign	0.27
R5616:Vmn2r59	UTSW	7	42058767	splice site	probably null
R5625:Vmn2r59	UTSW	7	42046460	missense	probably benign	0.03
R5696:Vmn2r59	UTSW	7	42046044	missense	probably benign	0.00
R5982:Vmn2r59	UTSW	7	42046067	missense	probably benign	0.00
R6106:Vmn2r59	UTSW	7	42012325	nonsense	probably null
R6196:Vmn2r59	UTSW	7	42012255	missense	probably benign	0.36
R6228:Vmn2r59	UTSW	7	42042411	critical splice donor site	probably null
R6590:Vmn2r59	UTSW	7	42046466	missense	probably damaging	1.00
R6625:Vmn2r59	UTSW	7	42043753	missense	probably benign	0.02
R6690:Vmn2r59	UTSW	7	42046466	missense	probably damaging	1.00
R6768:Vmn2r59	UTSW	7	42011968	missense	probably benign	0.17
R6830:Vmn2r59	UTSW	7	42043747	missense	probably benign	0.10
R6859:Vmn2r59	UTSW	7	42043853	missense	probably damaging	1.00
R7034:Vmn2r59	UTSW	7	42046220	missense	probably benign	0.03
R7036:Vmn2r59	UTSW	7	42046220	missense	probably benign	0.03
R7145:Vmn2r59	UTSW	7	42045764	missense	probably damaging	1.00
R7556:Vmn2r59	UTSW	7	42045809	missense	probably damaging	1.00
R7733:Vmn2r59	UTSW	7	42012019	missense	probably benign	0.17
R7770:Vmn2r59	UTSW	7	42058912	missense	probably damaging	1.00
R7812:Vmn2r59	UTSW	7	42045772	nonsense	probably null
R7867:Vmn2r59	UTSW	7	42012283	missense	probably damaging	1.00
R7975:Vmn2r59	UTSW	7	42043775	missense	probably damaging	1.00
R7999:Vmn2r59	UTSW	7	42046832	missense	probably damaging	1.00
R8267:Vmn2r59	UTSW	7	42012097	missense	probably damaging	0.97
R8367:Vmn2r59	UTSW	7	42011823	missense	probably benign	0.44
R9106:Vmn2r59	UTSW	7	42046460	missense	probably benign	0.03
R9135:Vmn2r59	UTSW	7	42043701	missense	probably benign	0.33
R9135:Vmn2r59	UTSW	7	42043703	missense
R9234:Vmn2r59	UTSW	7	42012483	missense	possibly damaging	0.67
R9273:Vmn2r59	UTSW	7	42045862	nonsense	probably null
R9432:Vmn2r59	UTSW	7	42046830	missense	probably damaging	1.00
R9433:Vmn2r59	UTSW	7	42046166	missense	probably damaging	0.99
R9616:Vmn2r59	UTSW	7	42011875	missense	probably damaging	1.00
R9654:Vmn2r59	UTSW	7	42043793	missense	probably benign	0.10
R9741:Vmn2r59	UTSW	7	42058785	missense	probably damaging	0.99
X0025:Vmn2r59	UTSW	7	42045941	missense	probably damaging	1.00
Z1088:Vmn2r59	UTSW	7	42012414	missense	possibly damaging	0.85
Z1176:Vmn2r59	UTSW	7	42042517	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GACCATGTTCAGCCTGAGTATC -3'
(R):5'- AGAACCCTTGGGAATGGCAC -3'

Sequencing Primer
(F):5'- GCCTGAGTATCTTGATCAATGAAGG -3'
(R):5'- TTGGGAATGGCACTCACC -3'

Posted On

2015-10-08