Mutagenetix > Incidental Mutation

Incidental Mutation 'R4617:Zfp352'

351103

Institutional Source

Beutler Lab

Gene Symbol

Zfp352

Ensembl Gene

ENSMUSG00000070902

Gene Name

zinc finger protein 352

Synonyms

2czf48

MMRRC Submission

041828-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4617 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

90107057-90113924 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 90113318 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Arginine at position 486 (K486R)

Ref Sequence

ENSEMBL: ENSMUSP00000102746 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080541] [ENSMUST00000107129]

AlphaFold

A2AML7

Predicted Effect

probably benign
Transcript: ENSMUST00000080541
AA Change: K486R

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000079383
Gene: ENSMUSG00000070902
AA Change: K486R

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107129
AA Change: K486R

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000102746
Gene: ENSMUSG00000070902
AA Change: K486R

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 94.9%

Validation Efficiency

96% (66/69)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	T	C	1: 71,369,493 (GRCm39)	I363V	probably benign	Het
Acaa1a	T	A	9: 119,178,006 (GRCm39)	S279R	probably damaging	Het
Akr1cl	A	G	1: 65,060,550 (GRCm39)	C156R	probably damaging	Het
Arhgap11a	G	A	2: 113,664,423 (GRCm39)	T620M	probably benign	Het
Brinp1	A	G	4: 68,681,198 (GRCm39)	I444T	possibly damaging	Het
Ccdc150	A	G	1: 54,394,913 (GRCm39)	I760V	probably benign	Het
Cdh26	G	A	2: 178,102,435 (GRCm39)		probably benign	Het
Cpd	A	G	11: 76,731,441 (GRCm39)	L255P	probably damaging	Het
Cyfip2	A	G	11: 46,144,845 (GRCm39)	Y670H	probably damaging	Het
Disp2	G	A	2: 118,620,643 (GRCm39)	M458I	probably benign	Het
Dot1l	T	C	10: 80,620,918 (GRCm39)	I563T	probably damaging	Het
Egf	A	T	3: 129,484,442 (GRCm39)	S1001T	probably benign	Het
Elfn1	A	G	5: 139,957,764 (GRCm39)	Y256C	probably damaging	Het
Exoc4	C	T	6: 33,839,139 (GRCm39)	T725I	probably benign	Het
Fam168b	A	G	1: 34,859,063 (GRCm39)	V72A	possibly damaging	Het
Fbxw20	C	T	9: 109,046,631 (GRCm39)	S443N	probably damaging	Het
Flacc1	A	T	1: 58,700,601 (GRCm39)	D301E	probably benign	Het
Flrt2	T	C	12: 95,747,003 (GRCm39)	V447A	possibly damaging	Het
Gm4846	A	G	1: 166,323,550 (GRCm39)	S58P	probably damaging	Het
Gpr20	T	C	15: 73,567,585 (GRCm39)	N268S	probably benign	Het
Lpcat2b	T	C	5: 107,581,865 (GRCm39)	L398P	possibly damaging	Het
Mapk8ip3	G	A	17: 25,123,761 (GRCm39)	P587L	probably damaging	Het
Mfap4	C	A	11: 61,376,335 (GRCm39)		probably benign	Het
Mplkipl1	C	T	19: 61,164,364 (GRCm39)	G24R	unknown	Het
Mrpl38	T	C	11: 116,023,278 (GRCm39)	D325G	probably damaging	Het
Mtmr12	A	G	15: 12,270,132 (GRCm39)	E430G	probably damaging	Het
Mup18	T	A	4: 61,590,154 (GRCm39)	I125F	possibly damaging	Het
Ogdhl	A	G	14: 32,047,842 (GRCm39)	R31G	probably benign	Het
Or13f5	A	T	4: 52,825,399 (GRCm39)	M1L	probably benign	Het
Pcdh1	C	A	18: 38,330,913 (GRCm39)	V697L	probably benign	Het
Pcdhga9	T	C	18: 37,871,553 (GRCm39)	Y461H	probably damaging	Het
Pdzrn4	A	G	15: 92,667,723 (GRCm39)	Y625C	probably damaging	Het
Pkd2l1	C	A	19: 44,142,573 (GRCm39)	A490S	probably damaging	Het
Poglut2	A	C	1: 44,149,180 (GRCm39)	F453V	probably damaging	Het
Ptprn	T	A	1: 75,228,931 (GRCm39)	D828V	possibly damaging	Het
Rgs12	T	A	5: 35,177,700 (GRCm39)	W97R	probably damaging	Het
Rnf10	T	A	5: 115,386,762 (GRCm39)	Q508L	probably damaging	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Sec24b	A	T	3: 129,834,413 (GRCm39)	S126T	possibly damaging	Het
Slc6a5	A	G	7: 49,561,768 (GRCm39)	N100S	probably benign	Het
Speer1j	T	C	5: 11,553,798 (GRCm39)	S7P	probably benign	Het
Stam2	A	T	2: 52,605,716 (GRCm39)	D167E	probably benign	Het
Stambp	G	A	6: 83,538,960 (GRCm39)	Q147*	probably null	Het
Tbc1d32	A	G	10: 56,047,000 (GRCm39)	V556A	possibly damaging	Het
Tll2	T	C	19: 41,087,075 (GRCm39)	D592G	probably benign	Het
Tmem199	A	T	11: 78,400,508 (GRCm39)		probably benign	Het
Traip	T	A	9: 107,847,218 (GRCm39)	N352K	probably benign	Het
Trmt1l	C	T	1: 151,329,799 (GRCm39)	Q581*	probably null	Het
Usp54	C	A	14: 20,600,406 (GRCm39)	A1444S	probably benign	Het
Vsig1	G	T	X: 139,827,135 (GRCm39)	A95S	probably benign	Het
Xdh	G	A	17: 74,225,389 (GRCm39)	T471I	probably damaging	Het
Zdhhc19	A	G	16: 32,316,494 (GRCm39)	D83G	probably damaging	Het
Zfp451	A	G	1: 33,841,752 (GRCm39)		probably benign	Het
Zfp963	A	T	8: 70,195,944 (GRCm39)	S170T	probably benign	Het
Zfp970	A	G	2: 177,167,961 (GRCm39)	I512V	probably benign	Het
Zfp990	A	G	4: 145,263,616 (GRCm39)	I205V	possibly damaging	Het

Other mutations in Zfp352

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01963:Zfp352	APN	4	90,112,391 (GRCm39)	missense	possibly damaging	0.95
IGL02252:Zfp352	APN	4	90,112,367 (GRCm39)	missense	probably benign	0.02
IGL03156:Zfp352	APN	4	90,112,324 (GRCm39)	missense	possibly damaging	0.57
IGL03167:Zfp352	APN	4	90,112,939 (GRCm39)	missense	probably damaging	0.99
IGL03190:Zfp352	APN	4	90,111,994 (GRCm39)	missense	possibly damaging	0.94
IGL03335:Zfp352	APN	4	90,112,583 (GRCm39)	missense	probably damaging	0.99
R0051:Zfp352	UTSW	4	90,112,522 (GRCm39)	missense	probably damaging	0.99
R0403:Zfp352	UTSW	4	90,113,246 (GRCm39)	missense	possibly damaging	0.60
R0550:Zfp352	UTSW	4	90,112,927 (GRCm39)	missense	probably damaging	0.99
R0671:Zfp352	UTSW	4	90,112,156 (GRCm39)	missense	probably benign
R1034:Zfp352	UTSW	4	90,112,393 (GRCm39)	missense	possibly damaging	0.94
R1754:Zfp352	UTSW	4	90,112,046 (GRCm39)	missense	probably benign	0.23
R2016:Zfp352	UTSW	4	90,113,408 (GRCm39)	missense	probably benign	0.42
R2064:Zfp352	UTSW	4	90,113,357 (GRCm39)	missense	probably benign	0.08
R2308:Zfp352	UTSW	4	90,113,480 (GRCm39)	missense	probably benign	0.00
R3552:Zfp352	UTSW	4	90,113,339 (GRCm39)	missense	probably benign	0.33
R3794:Zfp352	UTSW	4	90,113,386 (GRCm39)	missense	probably damaging	1.00
R3795:Zfp352	UTSW	4	90,113,386 (GRCm39)	missense	probably damaging	1.00
R4135:Zfp352	UTSW	4	90,113,261 (GRCm39)	missense	probably damaging	0.96
R4356:Zfp352	UTSW	4	90,112,071 (GRCm39)	missense	possibly damaging	0.91
R4409:Zfp352	UTSW	4	90,113,401 (GRCm39)	missense	probably benign	0.00
R4590:Zfp352	UTSW	4	90,112,772 (GRCm39)	missense	probably damaging	0.98
R4614:Zfp352	UTSW	4	90,113,318 (GRCm39)	missense	probably benign	0.00
R4618:Zfp352	UTSW	4	90,113,318 (GRCm39)	missense	probably benign	0.00
R4741:Zfp352	UTSW	4	90,113,177 (GRCm39)	missense	possibly damaging	0.94
R4931:Zfp352	UTSW	4	90,112,541 (GRCm39)	missense	probably damaging	0.98
R4959:Zfp352	UTSW	4	90,112,376 (GRCm39)	missense	probably benign	0.01
R4973:Zfp352	UTSW	4	90,112,376 (GRCm39)	missense	probably benign	0.01
R5167:Zfp352	UTSW	4	90,112,453 (GRCm39)	missense	possibly damaging	0.94
R5260:Zfp352	UTSW	4	90,112,697 (GRCm39)	missense	probably damaging	0.99
R5524:Zfp352	UTSW	4	90,113,341 (GRCm39)	missense	possibly damaging	0.95
R5942:Zfp352	UTSW	4	90,113,307 (GRCm39)	missense	probably damaging	0.98
R6802:Zfp352	UTSW	4	90,113,437 (GRCm39)	missense	probably benign	0.33
R6819:Zfp352	UTSW	4	90,112,936 (GRCm39)	missense	probably benign
R7072:Zfp352	UTSW	4	90,112,661 (GRCm39)	missense	probably benign	0.00
R7099:Zfp352	UTSW	4	90,113,117 (GRCm39)	missense	probably benign	0.00
R7569:Zfp352	UTSW	4	90,111,896 (GRCm39)	missense	possibly damaging	0.77
R7645:Zfp352	UTSW	4	90,113,014 (GRCm39)	missense	probably benign	0.13
R7705:Zfp352	UTSW	4	90,113,512 (GRCm39)	missense	possibly damaging	0.94
R8424:Zfp352	UTSW	4	90,112,480 (GRCm39)	missense	possibly damaging	0.87
R9180:Zfp352	UTSW	4	90,113,118 (GRCm39)	missense	probably benign	0.38
R9378:Zfp352	UTSW	4	90,112,575 (GRCm39)	missense	probably benign	0.13
R9509:Zfp352	UTSW	4	90,112,943 (GRCm39)	missense	probably damaging	0.99
R9623:Zfp352	UTSW	4	90,113,128 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ATGTGGGTGAATCCTCATACCC -3'
(R):5'- AGCATGTTTCTTCTCCAGATGC -3'

Sequencing Primer
(F):5'- TCGGCTTCCAGTTTAGTCCAGAG -3'
(R):5'- CCAGATGCTTTTTGAGATAAAGCAG -3'

Posted On

2015-10-08