Mutagenetix > Incidental Mutation

Incidental Mutation 'R0269:Laptm5'

35134

Institutional Source

Beutler Lab

Gene Symbol

Laptm5

Ensembl Gene

ENSMUSG00000028581

Gene Name

lysosomal-associated protein transmembrane 5

Synonyms

MMRRC Submission

038495-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0269 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

130640627-130663459 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 130658127 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Tyrosine at position 185 (N185Y)

Ref Sequence

ENSEMBL: ENSMUSP00000118415 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000151698] [ENSMUST00000156225]

AlphaFold

Q61168

Predicted Effect

unknown
Transcript: ENSMUST00000030316
AA Change: N187Y

SMART Domains

Protein: ENSMUSP00000030316
Gene: ENSMUSG00000028581
AA Change: N187Y

Domain	Start	End	E-Value	Type
Pfam:Mtp	31	263	1.7e-117	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123550

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125332

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136082

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143450

Predicted Effect

probably benign
Transcript: ENSMUST00000151698
AA Change: N185Y

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000118415
Gene: ENSMUSG00000028581
AA Change: N185Y

Domain	Start	End	E-Value	Type
Pfam:Mtp	28	260	7.1e-118	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156170

Predicted Effect

unknown
Transcript: ENSMUST00000156742
AA Change: N181Y

SMART Domains

Protein: ENSMUSP00000123382
Gene: ENSMUSG00000028581
AA Change: N181Y

Domain	Start	End	E-Value	Type
Pfam:Mtp	25	238	2.9e-104	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000156225

SMART Domains

Protein: ENSMUSP00000121133
Gene: ENSMUSG00000028581

Domain	Start	End	E-Value	Type
Pfam:Mtp	28	107	1.3e-28	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 98.7%
3x: 97.8%
10x: 96.1%
20x: 93.6%

Validation Efficiency

100% (59/59)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a transmembrane receptor that is associated with lysosomes. The encoded protein, also known as E3 protein, may play a role in hematopoiesis. [provided by RefSeq, Feb 2009]
PHENOTYPE: Mice homozygous for a null allele exhibit increased T cell proliferation, increased IL-2 production and a prolognged type IV hypersensitivity response. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5031439G07Rik	C	T	15: 84,838,201 (GRCm39)	V231I	possibly damaging	Het
Abca1	T	C	4: 53,044,228 (GRCm39)	D1798G	probably benign	Het
Adcy2	T	A	13: 68,826,725 (GRCm39)	K660*	probably null	Het
Alk	G	T	17: 72,910,578 (GRCm39)	P43T	probably damaging	Het
Amhr2	T	C	15: 102,355,503 (GRCm39)	C189R	probably benign	Het
Arrb1	T	C	7: 99,243,884 (GRCm39)	L278P	probably damaging	Het
AW551984	T	C	9: 39,511,246 (GRCm39)	Y153C	probably damaging	Het
Bpifb1	A	G	2: 154,054,867 (GRCm39)	D253G	possibly damaging	Het
Bpifb9b	C	T	2: 154,161,545 (GRCm39)	T559M	probably benign	Het
Cd46	T	G	1: 194,746,996 (GRCm39)	I339L	probably benign	Het
Cdkn2aip	A	G	8: 48,165,012 (GRCm39)	S234P	probably damaging	Het
Chil3	T	C	3: 106,063,072 (GRCm39)	K173E	probably benign	Het
Csf2rb2	G	T	15: 78,173,065 (GRCm39)	T265N	probably benign	Het
Cyp2c40	A	G	19: 39,762,340 (GRCm39)	F436L	probably damaging	Het
D130040H23Rik	T	C	8: 69,753,446 (GRCm39)	F24S	probably benign	Het
Defb12	G	T	8: 19,164,375 (GRCm39)	A34E	probably damaging	Het
Fam234a	A	T	17: 26,435,591 (GRCm39)	D264E	probably benign	Het
Fbxl17	A	C	17: 63,691,987 (GRCm39)	F42V	probably damaging	Het
Gldc	T	A	19: 30,096,002 (GRCm39)	I670F	probably damaging	Het
Guf1	A	C	5: 69,716,942 (GRCm39)	Q168P	probably damaging	Het
Hcn2	A	G	10: 79,570,075 (GRCm39)		probably benign	Het
Hddc2	A	G	10: 31,203,942 (GRCm39)	M190V	probably benign	Het
Kcnq2	T	C	2: 180,738,767 (GRCm39)	E294G	probably benign	Het
Kdelr1	T	A	7: 45,523,463 (GRCm39)		probably benign	Het
Kidins220	T	A	12: 25,090,511 (GRCm39)	H1158Q	probably damaging	Het
Mgat4a	A	G	1: 37,529,388 (GRCm39)	Y164H	possibly damaging	Het
Mlh3	C	A	12: 85,315,179 (GRCm39)	V336L	probably benign	Het
Myadm	A	G	7: 3,345,273 (GRCm39)	T12A	unknown	Het
Nol8	T	C	13: 49,807,921 (GRCm39)	F46L	possibly damaging	Het
Ntrk1	T	C	3: 87,691,240 (GRCm39)	D308G	possibly damaging	Het
Oog3	A	T	4: 143,886,784 (GRCm39)	V112D	probably benign	Het
Or4a66	A	G	2: 88,531,040 (GRCm39)	V211A	probably damaging	Het
Or5af2	T	C	11: 58,707,975 (GRCm39)	V47A	probably damaging	Het
Or5m9b	A	G	2: 85,905,485 (GRCm39)	M134V	probably benign	Het
Or8g34	T	C	9: 39,373,090 (GRCm39)	M118T	probably damaging	Het
Or9s18	A	T	13: 65,300,692 (GRCm39)	Y218F	possibly damaging	Het
Pramel14	A	G	4: 143,720,088 (GRCm39)		probably benign	Het
Prss39	A	T	1: 34,539,279 (GRCm39)	H173L	probably damaging	Het
Rabl6	A	G	2: 25,476,878 (GRCm39)		probably null	Het
Recql5	T	C	11: 115,819,050 (GRCm39)	D172G	possibly damaging	Het
Reln	T	C	5: 22,125,535 (GRCm39)	D2716G	probably damaging	Het
Rgs7	A	G	1: 175,098,386 (GRCm39)	S58P	possibly damaging	Het
Sema6d	T	A	2: 124,502,665 (GRCm39)	F583L	possibly damaging	Het
Sgsm1	T	C	5: 113,434,795 (GRCm39)		probably null	Het
Slc22a19	A	T	19: 7,686,986 (GRCm39)		probably benign	Het
Slc6a21	T	A	7: 44,936,332 (GRCm39)	Y428*	probably null	Het
Smarca4	T	G	9: 21,547,497 (GRCm39)	M260R	probably benign	Het
Smg6	C	A	11: 75,053,757 (GRCm39)	T1413K	probably benign	Het
Spata17	T	C	1: 186,830,069 (GRCm39)	I322V	probably benign	Het
Stxbp1	A	C	2: 32,692,795 (GRCm39)	I407S	probably damaging	Het
Sult1d1	A	T	5: 87,712,661 (GRCm39)	I61N	probably damaging	Het
Sytl2	T	C	7: 90,052,228 (GRCm39)		probably benign	Het
Tm4sf5	T	A	11: 70,401,495 (GRCm39)	S165T	probably damaging	Het
Tmx2	T	C	2: 84,502,740 (GRCm39)	D256G	probably benign	Het
Trmt11	T	A	10: 30,463,485 (GRCm39)	H210L	probably benign	Het
Tut7	A	T	13: 59,964,669 (GRCm39)		probably null	Het
Ush2a	T	A	1: 188,542,373 (GRCm39)	M3313K	probably benign	Het
Zfp955b	A	T	17: 33,524,437 (GRCm39)	S43R	probably damaging	Het

Other mutations in Laptm5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1439:Laptm5	UTSW	4	130,653,520 (GRCm39)	splice site	probably benign
R2570:Laptm5	UTSW	4	130,659,358 (GRCm39)	missense	probably damaging	1.00
R4599:Laptm5	UTSW	4	130,643,316 (GRCm39)	intron	probably benign
R4696:Laptm5	UTSW	4	130,660,982 (GRCm39)	unclassified	probably benign
R4812:Laptm5	UTSW	4	130,640,749 (GRCm39)	splice site	probably null
R5381:Laptm5	UTSW	4	130,660,969 (GRCm39)	unclassified	probably benign
R8223:Laptm5	UTSW	4	130,653,511 (GRCm39)	critical splice donor site	probably null
R8353:Laptm5	UTSW	4	130,656,079 (GRCm39)	splice site	probably null
R9045:Laptm5	UTSW	4	130,655,955 (GRCm39)	missense
R9335:Laptm5	UTSW	4	130,656,839 (GRCm39)	missense
R9407:Laptm5	UTSW	4	130,655,990 (GRCm39)	missense
R9429:Laptm5	UTSW	4	130,655,961 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- CAGTTGCCAAGGTAGTGCATGAGTG -3'
(R):5'- AGCACATGAGGCCAGTGTCACAAG -3'

Sequencing Primer
(F):5'- agagagagaTCTGTGACGCT -3'
(R):5'- AGTACTCATGGCTTCACAGAC -3'

Posted On

2013-05-09