Mutagenetix > Incidental Mutation

Incidental Mutation 'R4654:Or5b21'

351590

Institutional Source

Beutler Lab

Gene Symbol

Or5b21

Ensembl Gene

ENSMUSG00000046272

Gene Name

olfactory receptor family 5 subfamily B member 21

Synonyms

Olfr1444, GA_x6K02T2RE5P-3191201-3192160, MOR202-4

MMRRC Submission

041914-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.246) question?

Stock #

R4654 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

12839141-12840100 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 12839596 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 152 (C152*)

Ref Sequence

ENSEMBL: ENSMUSP00000150212 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059675] [ENSMUST00000213606]

AlphaFold

Q8VFX2

Predicted Effect

probably null
Transcript: ENSMUST00000059675
AA Change: C152*

SMART Domains

Protein: ENSMUSP00000062460
Gene: ENSMUSG00000046272
AA Change: C152*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	308	1.6e-54	PFAM
Pfam:7tm_1	42	291	5.3e-23	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000213606
AA Change: C152*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.2%

Validation Efficiency

97% (98/101)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam3	A	T	8: 25,193,819 (GRCm39)	C398S	probably damaging	Het
Adamts10	A	G	17: 33,756,304 (GRCm39)	K316E	possibly damaging	Het
Ap1m1	T	C	8: 73,006,717 (GRCm39)	F238L	possibly damaging	Het
Aph1a	T	A	3: 95,803,088 (GRCm39)	D180E	probably benign	Het
Atp9b	A	G	18: 80,935,093 (GRCm39)	F201L	probably benign	Het
Btbd9	A	C	17: 30,704,561 (GRCm39)		probably benign	Het
C2cd5	T	C	6: 142,975,910 (GRCm39)	T768A	probably benign	Het
Casq1	C	T	1: 172,037,965 (GRCm39)		probably benign	Het
Cimap2	C	A	4: 106,467,612 (GRCm39)	M341I	probably benign	Het
Cltc	A	T	11: 86,617,196 (GRCm39)	M351K	probably benign	Het
Cnp	A	G	11: 100,469,877 (GRCm39)	E271G	possibly damaging	Het
Col22a1	T	C	15: 71,845,544 (GRCm39)	D406G	possibly damaging	Het
Cylc2	T	C	4: 51,228,279 (GRCm39)	S117P	probably benign	Het
Cyp2b13	T	C	7: 25,761,072 (GRCm39)	L43P	probably damaging	Het
Cyp3a16	A	G	5: 145,373,267 (GRCm39)	V500A	probably benign	Het
Dclk2	T	C	3: 86,743,683 (GRCm39)	D262G	probably damaging	Het
Dcun1d3	A	C	7: 119,458,742 (GRCm39)	Y98D	probably damaging	Het
Ddx39b	A	G	17: 35,472,464 (GRCm39)	*429W	probably null	Het
Dennd5b	T	C	6: 148,908,335 (GRCm39)	N986S	probably damaging	Het
Dipk1a	T	C	5: 108,057,982 (GRCm39)		probably null	Het
Dusp9	G	A	X: 72,684,378 (GRCm39)	R182Q	probably benign	Het
Ecpas	A	T	4: 58,834,523 (GRCm39)	I785N	possibly damaging	Het
Edil3	A	G	13: 89,437,589 (GRCm39)	K397E	probably damaging	Het
Ehd1	T	C	19: 6,326,994 (GRCm39)		probably benign	Het
Fam13a	A	T	6: 58,964,152 (GRCm39)	H93Q	probably benign	Het
Farp1	T	C	14: 121,513,716 (GRCm39)	I837T	possibly damaging	Het
Fbxl5	T	A	5: 43,922,771 (GRCm39)	I216F	probably damaging	Het
Gadl1	G	A	9: 115,770,408 (GRCm39)	E74K	probably damaging	Het
Gnpnat1	A	G	14: 45,618,436 (GRCm39)	V122A	probably damaging	Het
Hdac10	C	T	15: 89,011,036 (GRCm39)		probably benign	Het
Heatr5b	T	C	17: 79,128,130 (GRCm39)	S502G	possibly damaging	Het
Hr	C	A	14: 70,801,013 (GRCm39)	A695E	probably damaging	Het
Ift80	A	T	3: 68,825,870 (GRCm39)	I490N	possibly damaging	Het
Ipo11	A	G	13: 106,970,692 (GRCm39)		probably benign	Het
Iqch	T	G	9: 63,432,195 (GRCm39)	Y400S	probably damaging	Het
Jag2	C	T	12: 112,877,266 (GRCm39)	D702N	probably benign	Het
Kiss1r	T	A	10: 79,757,624 (GRCm39)	L326Q	probably damaging	Het
Lrrc42	A	T	4: 107,104,746 (GRCm39)	I73N	probably damaging	Het
Lrrc55	C	T	2: 85,026,880 (GRCm39)	G48D	possibly damaging	Het
Lrrk2	T	C	15: 91,649,884 (GRCm39)	S1674P	probably damaging	Het
Mctp2	A	G	7: 71,739,942 (GRCm39)	L816P	probably damaging	Het
Mipol1	A	T	12: 57,352,918 (GRCm39)	T86S	probably benign	Het
Mkrn3	C	T	7: 62,069,452 (GRCm39)	R113H	probably damaging	Het
Mmp1b	A	G	9: 7,370,849 (GRCm39)	V302A	probably benign	Het
Msc	A	T	1: 14,826,053 (GRCm39)		probably null	Het
Msmo1	C	A	8: 65,180,888 (GRCm39)	V9L	probably benign	Het
Nbeal2	G	A	9: 110,461,072 (GRCm39)	R1630C	probably damaging	Het
Nlgn1	C	T	3: 26,187,850 (GRCm39)	V12I	possibly damaging	Het
Npas3	T	C	12: 54,108,915 (GRCm39)		probably null	Het
Nr2e3	A	G	9: 59,856,355 (GRCm39)		probably benign	Het
Oca2	C	T	7: 55,978,560 (GRCm39)	A576V	probably benign	Het
Or6d12	T	C	6: 116,493,409 (GRCm39)	Y224H	probably damaging	Het
Or6z7	T	C	7: 6,484,045 (GRCm39)	T37A	probably benign	Het
Parp6	A	G	9: 59,548,383 (GRCm39)		probably null	Het
Pate13	T	A	9: 35,820,287 (GRCm39)	C4S	probably damaging	Het
Phlpp1	A	T	1: 106,267,231 (GRCm39)	M715L	probably benign	Het
Pi4k2a	G	A	19: 42,101,544 (GRCm39)		probably null	Het
Pik3ap1	A	G	19: 41,316,348 (GRCm39)	S305P	probably damaging	Het
Plcg2	G	A	8: 118,231,054 (GRCm39)	M45I	probably benign	Het
Ppm1m	A	T	9: 106,073,601 (GRCm39)	L317H	probably damaging	Het
Ppp1r21	T	G	17: 88,866,227 (GRCm39)	M341R	probably benign	Het
Prdx3	A	G	19: 60,853,674 (GRCm39)	V217A	possibly damaging	Het
Ptk2b	A	G	14: 66,400,496 (GRCm39)	V773A	possibly damaging	Het
Raly	T	C	2: 154,699,376 (GRCm39)	V60A	probably damaging	Het
Reps1	A	G	10: 17,990,148 (GRCm39)	D420G	probably damaging	Het
Rev1	A	T	1: 38,118,337 (GRCm39)		probably benign	Het
Rgs2	T	G	1: 143,878,650 (GRCm39)		probably benign	Het
Rlf	G	T	4: 121,007,798 (GRCm39)	T394K	probably benign	Het
Rptn	A	T	3: 93,304,792 (GRCm39)	R708S	possibly damaging	Het
Sec23b	T	A	2: 144,414,494 (GRCm39)	M402K	probably benign	Het
Skic2	A	G	17: 35,068,922 (GRCm39)	C26R	probably damaging	Het
Slx9	T	C	10: 77,325,860 (GRCm39)	M170V	possibly damaging	Het
Smpd4	A	G	16: 17,459,992 (GRCm39)		probably benign	Het
Synj2	A	G	17: 6,063,813 (GRCm39)	E434G	probably damaging	Het
Tatdn2	T	A	6: 113,684,326 (GRCm39)	F64I	probably benign	Het
Tex21	A	C	12: 76,263,860 (GRCm39)	H177Q	probably benign	Het
Tln1	T	A	4: 43,535,954 (GRCm39)	Q2077L	probably null	Het
Tnrc6c	T	C	11: 117,611,797 (GRCm39)	V145A	probably benign	Het
Ttn	C	T	2: 76,616,936 (GRCm39)		probably benign	Het
Uggt2	A	G	14: 119,269,670 (GRCm39)	F954S	possibly damaging	Het
Ugt2a1	A	G	5: 87,634,083 (GRCm39)	S175P	probably damaging	Het
Vcl	A	G	14: 21,035,820 (GRCm39)		probably null	Het
Vegfa	A	T	17: 46,336,176 (GRCm39)		probably benign	Het
Vmn2r68	G	A	7: 84,882,769 (GRCm39)	Q328*	probably null	Het
Vmn2r7	A	G	3: 64,626,864 (GRCm39)	Y142H	probably benign	Het
Wdr17	C	T	8: 55,134,434 (GRCm39)	G349R	probably damaging	Het
Ybx3	G	T	6: 131,347,290 (GRCm39)	R282S	probably damaging	Het
Zfp566	T	C	7: 29,777,194 (GRCm39)	H329R	probably damaging	Het
Zfp786	T	C	6: 47,797,868 (GRCm39)	I357V	probably benign	Het
Zfr2	C	A	10: 81,087,083 (GRCm39)		probably null	Het

Other mutations in Or5b21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01668:Or5b21	APN	19	12,839,231 (GRCm39)	missense	probably benign	0.00
IGL01963:Or5b21	APN	19	12,839,746 (GRCm39)	missense	probably benign	0.00
IGL02030:Or5b21	APN	19	12,839,799 (GRCm39)	missense	probably benign	0.00
IGL02178:Or5b21	APN	19	12,839,907 (GRCm39)	missense	possibly damaging	0.49
IGL02641:Or5b21	APN	19	12,839,566 (GRCm39)	nonsense	probably null
R0311:Or5b21	UTSW	19	12,839,233 (GRCm39)	missense	probably benign	0.01
R0543:Or5b21	UTSW	19	12,839,252 (GRCm39)	missense	probably benign	0.00
R0815:Or5b21	UTSW	19	12,840,008 (GRCm39)	missense	probably benign	0.00
R2034:Or5b21	UTSW	19	12,839,151 (GRCm39)	missense	possibly damaging	0.82
R2078:Or5b21	UTSW	19	12,839,751 (GRCm39)	missense	probably benign	0.05
R2431:Or5b21	UTSW	19	12,839,970 (GRCm39)	missense	probably damaging	1.00
R3032:Or5b21	UTSW	19	12,839,282 (GRCm39)	missense	probably benign	0.00
R3932:Or5b21	UTSW	19	12,839,994 (GRCm39)	missense	possibly damaging	0.95
R4498:Or5b21	UTSW	19	12,840,033 (GRCm39)	missense	probably damaging	1.00
R4708:Or5b21	UTSW	19	12,839,261 (GRCm39)	missense	probably benign	0.00
R4823:Or5b21	UTSW	19	12,839,180 (GRCm39)	missense	probably benign	0.04
R4938:Or5b21	UTSW	19	12,839,916 (GRCm39)	missense	probably damaging	1.00
R4980:Or5b21	UTSW	19	12,839,384 (GRCm39)	missense	probably benign
R5580:Or5b21	UTSW	19	12,839,168 (GRCm39)	missense	possibly damaging	0.59
R5622:Or5b21	UTSW	19	12,839,663 (GRCm39)	missense	probably benign	0.08
R5671:Or5b21	UTSW	19	12,839,171 (GRCm39)	missense	probably benign	0.02
R6149:Or5b21	UTSW	19	12,839,723 (GRCm39)	missense	probably benign	0.02
R6683:Or5b21	UTSW	19	12,840,014 (GRCm39)	missense	probably damaging	0.98
R7389:Or5b21	UTSW	19	12,839,981 (GRCm39)	missense	probably benign	0.04
R7392:Or5b21	UTSW	19	12,839,951 (GRCm39)	missense	probably benign	0.18
R7461:Or5b21	UTSW	19	12,839,141 (GRCm39)	start codon destroyed	probably benign	0.00
R7613:Or5b21	UTSW	19	12,839,141 (GRCm39)	start codon destroyed	probably benign	0.00
R7698:Or5b21	UTSW	19	12,840,077 (GRCm39)	missense	possibly damaging	0.69
R7717:Or5b21	UTSW	19	12,839,159 (GRCm39)	missense	probably benign	0.07
R7892:Or5b21	UTSW	19	12,839,843 (GRCm39)	nonsense	probably null
R9151:Or5b21	UTSW	19	12,839,976 (GRCm39)	missense	probably damaging	1.00
R9656:Or5b21	UTSW	19	12,839,247 (GRCm39)	missense	probably damaging	1.00
Z1088:Or5b21	UTSW	19	12,839,648 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCTATAATGGGTGTGCAGC -3'
(R):5'- CGTCCCTCAGAAGACTTCATG -3'

Sequencing Primer
(F):5'- ATAATGGGTGTGCAGCTCAATTTTTC -3'
(R):5'- GTCCCTCAGAAGACTTCATGTTTTG -3'

Posted On

2015-10-08