Incidental Mutation 'R4643:Zfp663'
| ID |
351715 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp663
|
| Ensembl Gene |
ENSMUSG00000056824 |
| Gene Name |
zinc finger protein 663 |
| Synonyms |
LOC381405, Gm1008 |
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.059)
|
| Stock # |
R4643 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
165193217-165210649 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 165194925 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Histidine to Glutamine
at position 431
(H431Q)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000099374
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000073062]
[ENSMUST00000103085]
[ENSMUST00000141140]
|
| AlphaFold |
Q6NXM6 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000073062
AA Change: H431Q
PolyPhen 2
Score 0.243 (Sensitivity: 0.91; Specificity: 0.88)
|
| SMART Domains |
Protein: ENSMUSP00000072813
Gene: ENSMUSG00000056824
AA Change: H431Q
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
8 |
68 |
1.97e-31 |
SMART |
|
ZnF_C2H2
|
205 |
227 |
3.47e1 |
SMART |
|
ZnF_C2H2
|
472 |
494 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
500 |
522 |
2.99e-4 |
SMART |
|
ZnF_C2H2
|
528 |
550 |
2.43e-4 |
SMART |
|
ZnF_C2H2
|
556 |
578 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
584 |
606 |
3.95e-4 |
SMART |
|
ZnF_C2H2
|
612 |
635 |
8.6e-5 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000103085
AA Change: H431Q
PolyPhen 2
Score 0.243 (Sensitivity: 0.91; Specificity: 0.88)
|
| SMART Domains |
Protein: ENSMUSP00000099374
Gene: ENSMUSG00000056824
AA Change: H431Q
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
8 |
68 |
1.97e-31 |
SMART |
|
ZnF_C2H2
|
205 |
227 |
3.47e1 |
SMART |
|
ZnF_C2H2
|
472 |
494 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
500 |
522 |
2.99e-4 |
SMART |
|
ZnF_C2H2
|
528 |
550 |
2.43e-4 |
SMART |
|
ZnF_C2H2
|
556 |
578 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
584 |
606 |
3.95e-4 |
SMART |
|
ZnF_C2H2
|
612 |
635 |
8.6e-5 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000136482
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000141140
|
| SMART Domains |
Protein: ENSMUSP00000115254
Gene: ENSMUSG00000056824
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
8 |
68 |
1.97e-31 |
SMART |
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.2%
- 20x: 95.1%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 41 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4833420G17Rik |
A |
G |
13: 119,611,396 (GRCm39) |
N398D |
probably damaging |
Het |
| Adamtsl4 |
G |
A |
3: 95,591,929 (GRCm39) |
A58V |
possibly damaging |
Het |
| Anapc2 |
T |
A |
2: 25,166,406 (GRCm39) |
V105E |
probably benign |
Het |
| C3ar1 |
A |
T |
6: 122,827,933 (GRCm39) |
C95S |
probably damaging |
Het |
| Ccdc87 |
G |
A |
19: 4,891,877 (GRCm39) |
G790R |
probably damaging |
Het |
| Cenpf |
A |
T |
1: 189,391,786 (GRCm39) |
M682K |
probably benign |
Het |
| Cyp2j13 |
T |
C |
4: 95,945,161 (GRCm39) |
Q289R |
possibly damaging |
Het |
| Dclk2 |
A |
T |
3: 86,713,487 (GRCm39) |
M453K |
possibly damaging |
Het |
| Dscam |
G |
A |
16: 96,486,501 (GRCm39) |
T1058M |
probably damaging |
Het |
| Gas2l3 |
CACTCGTCATACT |
CACT |
10: 89,266,820 (GRCm39) |
|
probably benign |
Het |
| Grip1 |
A |
G |
10: 119,856,006 (GRCm39) |
N659S |
probably damaging |
Het |
| Hectd4 |
A |
G |
5: 121,487,118 (GRCm39) |
K3371R |
possibly damaging |
Het |
| Il1a |
T |
A |
2: 129,146,623 (GRCm39) |
T157S |
probably benign |
Het |
| Il23r |
A |
G |
6: 67,400,977 (GRCm39) |
V451A |
probably benign |
Het |
| Iqch |
T |
C |
9: 63,502,084 (GRCm39) |
T40A |
probably benign |
Het |
| Irag2 |
A |
T |
6: 145,113,786 (GRCm39) |
D318V |
probably benign |
Het |
| Kazald1 |
G |
T |
19: 45,066,788 (GRCm39) |
V196L |
probably benign |
Het |
| L1td1 |
T |
C |
4: 98,626,120 (GRCm39) |
S772P |
probably damaging |
Het |
| Lgi1 |
A |
T |
19: 38,289,158 (GRCm39) |
D145V |
probably damaging |
Het |
| Lrp12 |
A |
T |
15: 39,735,418 (GRCm39) |
L838Q |
probably damaging |
Het |
| Mrgprf |
C |
A |
7: 144,862,242 (GRCm39) |
P268Q |
probably benign |
Het |
| Myef2 |
T |
C |
2: 124,958,731 (GRCm39) |
K66R |
possibly damaging |
Het |
| Myo3b |
A |
G |
2: 70,069,186 (GRCm39) |
D475G |
possibly damaging |
Het |
| Numa1 |
C |
G |
7: 101,649,872 (GRCm39) |
|
probably null |
Het |
| Or6c214 |
T |
C |
10: 129,590,824 (GRCm39) |
E165G |
probably damaging |
Het |
| Prdm8 |
T |
C |
5: 98,332,446 (GRCm39) |
S116P |
possibly damaging |
Het |
| Pyroxd1 |
C |
G |
6: 142,300,467 (GRCm39) |
S199* |
probably null |
Het |
| R3hcc1l |
G |
T |
19: 42,551,239 (GRCm39) |
V79F |
probably benign |
Het |
| Rasal1 |
C |
T |
5: 120,817,029 (GRCm39) |
T779I |
probably benign |
Het |
| Scaper |
A |
C |
9: 55,745,463 (GRCm39) |
F596V |
probably damaging |
Het |
| Slco2b1 |
C |
T |
7: 99,316,214 (GRCm39) |
V439M |
probably benign |
Het |
| Slco6c1 |
A |
T |
1: 96,990,149 (GRCm39) |
D680E |
probably benign |
Het |
| Smurf1 |
A |
T |
5: 144,816,179 (GRCm39) |
F725L |
probably damaging |
Het |
| Snd1 |
A |
G |
6: 28,880,248 (GRCm39) |
E674G |
probably benign |
Het |
| Srcap |
C |
T |
7: 127,140,948 (GRCm39) |
P1515L |
probably damaging |
Het |
| Sycp2l |
A |
G |
13: 41,296,941 (GRCm39) |
M341V |
probably benign |
Het |
| Tmprss6 |
T |
A |
15: 78,329,556 (GRCm39) |
I492F |
probably damaging |
Het |
| Trip10 |
G |
T |
17: 57,568,658 (GRCm39) |
E416* |
probably null |
Het |
| Tstd2 |
T |
C |
4: 46,129,297 (GRCm39) |
D177G |
possibly damaging |
Het |
| Ugt1a5 |
A |
T |
1: 88,094,147 (GRCm39) |
N125I |
possibly damaging |
Het |
| Vmn2r7 |
G |
T |
3: 64,623,825 (GRCm39) |
S165Y |
probably damaging |
Het |
|
| Other mutations in Zfp663 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00720:Zfp663
|
APN |
2 |
165,194,525 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01382:Zfp663
|
APN |
2 |
165,200,935 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02007:Zfp663
|
APN |
2 |
165,200,993 (GRCm39) |
missense |
probably benign |
0.12 |
|
IGL02164:Zfp663
|
APN |
2 |
165,200,968 (GRCm39) |
nonsense |
probably null |
|
|
IGL02506:Zfp663
|
APN |
2 |
165,195,871 (GRCm39) |
missense |
probably benign |
0.35 |
|
IGL03173:Zfp663
|
APN |
2 |
165,194,419 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0735:Zfp663
|
UTSW |
2 |
165,200,995 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R1395:Zfp663
|
UTSW |
2 |
165,194,492 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1402:Zfp663
|
UTSW |
2 |
165,195,890 (GRCm39) |
missense |
probably benign |
0.14 |
|
R1402:Zfp663
|
UTSW |
2 |
165,195,890 (GRCm39) |
missense |
probably benign |
0.14 |
|
R1503:Zfp663
|
UTSW |
2 |
165,194,573 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1587:Zfp663
|
UTSW |
2 |
165,195,437 (GRCm39) |
missense |
probably benign |
|
|
R1854:Zfp663
|
UTSW |
2 |
165,195,211 (GRCm39) |
missense |
probably benign |
0.18 |
|
R1867:Zfp663
|
UTSW |
2 |
165,194,651 (GRCm39) |
missense |
possibly damaging |
0.74 |
|
R3031:Zfp663
|
UTSW |
2 |
165,195,616 (GRCm39) |
nonsense |
probably null |
|
|
R4691:Zfp663
|
UTSW |
2 |
165,201,050 (GRCm39) |
intron |
probably benign |
|
|
R4977:Zfp663
|
UTSW |
2 |
165,195,731 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R5135:Zfp663
|
UTSW |
2 |
165,195,590 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R5151:Zfp663
|
UTSW |
2 |
165,195,113 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5639:Zfp663
|
UTSW |
2 |
165,194,929 (GRCm39) |
missense |
probably benign |
0.03 |
|
R5763:Zfp663
|
UTSW |
2 |
165,200,355 (GRCm39) |
nonsense |
probably null |
|
|
R6776:Zfp663
|
UTSW |
2 |
165,200,935 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6929:Zfp663
|
UTSW |
2 |
165,195,178 (GRCm39) |
missense |
probably benign |
|
|
R6998:Zfp663
|
UTSW |
2 |
165,195,922 (GRCm39) |
missense |
possibly damaging |
0.74 |
|
R7035:Zfp663
|
UTSW |
2 |
165,195,023 (GRCm39) |
missense |
probably benign |
0.36 |
|
R7169:Zfp663
|
UTSW |
2 |
165,194,359 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7529:Zfp663
|
UTSW |
2 |
165,194,728 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7790:Zfp663
|
UTSW |
2 |
165,194,453 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8087:Zfp663
|
UTSW |
2 |
165,195,679 (GRCm39) |
missense |
probably benign |
0.20 |
|
R8715:Zfp663
|
UTSW |
2 |
165,194,644 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8934:Zfp663
|
UTSW |
2 |
165,194,714 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8966:Zfp663
|
UTSW |
2 |
165,194,958 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9257:Zfp663
|
UTSW |
2 |
165,195,974 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9278:Zfp663
|
UTSW |
2 |
165,202,010 (GRCm39) |
critical splice acceptor site |
probably null |
|
|
R9524:Zfp663
|
UTSW |
2 |
165,195,607 (GRCm39) |
missense |
probably damaging |
0.99 |
|
RF004:Zfp663
|
UTSW |
2 |
165,200,363 (GRCm39) |
missense |
probably benign |
0.00 |
|
Z1177:Zfp663
|
UTSW |
2 |
165,195,033 (GRCm39) |
missense |
probably damaging |
0.99 |
|
| Predicted Primers |
PCR Primer
(F):5'- TGGACTTGACACAGAACGAC -3'
(R):5'- CCGGAAAACTTGCACAGCAG -3'
Sequencing Primer
(F):5'- GCAGAAAGTTTTCCCACATTCG -3'
(R):5'- GCAGAAAAACCCAAGTGTGC -3'
|
| Posted On |
2015-10-08 |
Incidental Mutation