Incidental Mutation 'R4643:Tstd2'
ID351719
Institutional Source Beutler Lab
Gene Symbol Tstd2
Ensembl Gene ENSMUSG00000035495
Gene Namethiosulfate sulfurtransferase (rhodanese)-like domain containing 2
Synonyms
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.136) question?
Stock #R4643 (G1)
Quality Score225
Status Not validated
Chromosome4
Chromosomal Location46114746-46138694 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 46129297 bp
ZygosityHeterozygous
Amino Acid Change Aspartic acid to Glycine at position 177 (D177G)
Ref Sequence ENSEMBL: ENSMUSP00000103401 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000107770] [ENSMUST00000107772] [ENSMUST00000129929] [ENSMUST00000144495] [ENSMUST00000147837]
Predicted Effect possibly damaging
Transcript: ENSMUST00000107770
AA Change: D177G

PolyPhen 2 Score 0.917 (Sensitivity: 0.81; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000103399
Gene: ENSMUSG00000035495
AA Change: D177G

DomainStartEndE-ValueType
low complexity region 44 64 N/A INTRINSIC
RHOD 290 392 2.25e-11 SMART
Pfam:Rhodanese_C 396 459 2.4e-21 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000107772
AA Change: D177G

PolyPhen 2 Score 0.917 (Sensitivity: 0.81; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000103401
Gene: ENSMUSG00000035495
AA Change: D177G

DomainStartEndE-ValueType
low complexity region 44 64 N/A INTRINSIC
RHOD 290 392 2.25e-11 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000129929
SMART Domains Protein: ENSMUSP00000125707
Gene: ENSMUSG00000035495

DomainStartEndE-ValueType
low complexity region 46 57 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000137399
Predicted Effect probably benign
Transcript: ENSMUST00000144495
SMART Domains Protein: ENSMUSP00000117990
Gene: ENSMUSG00000035495

DomainStartEndE-ValueType
low complexity region 44 64 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000147837
SMART Domains Protein: ENSMUSP00000115473
Gene: ENSMUSG00000035495

DomainStartEndE-ValueType
low complexity region 44 64 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000160008
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.2%
  • 20x: 95.1%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 41 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4833420G17Rik A G 13: 119,474,860 N398D probably damaging Het
Adamtsl4 G A 3: 95,684,619 A58V possibly damaging Het
Anapc2 T A 2: 25,276,394 V105E probably benign Het
C3ar1 A T 6: 122,850,974 C95S probably damaging Het
Ccdc87 G A 19: 4,841,849 G790R probably damaging Het
Cenpf A T 1: 189,659,589 M682K probably benign Het
Cyp2j13 T C 4: 96,056,924 Q289R possibly damaging Het
Dclk2 A T 3: 86,806,180 M453K possibly damaging Het
Dscam G A 16: 96,685,301 T1058M probably damaging Het
Gas2l3 CACTCGTCATACT CACT 10: 89,430,958 probably benign Het
Grip1 A G 10: 120,020,101 N659S probably damaging Het
Hectd4 A G 5: 121,349,055 K3371R possibly damaging Het
Il1a T A 2: 129,304,703 T157S probably benign Het
Il23r A G 6: 67,423,993 V451A probably benign Het
Iqch T C 9: 63,594,802 T40A probably benign Het
Kazald1 G T 19: 45,078,349 V196L probably benign Het
L1td1 T C 4: 98,737,883 S772P probably damaging Het
Lgi1 A T 19: 38,300,710 D145V probably damaging Het
Lrmp A T 6: 145,168,060 D318V probably benign Het
Lrp12 A T 15: 39,872,022 L838Q probably damaging Het
Mrgprf C A 7: 145,308,505 P268Q probably benign Het
Myef2 T C 2: 125,116,811 K66R possibly damaging Het
Myo3b A G 2: 70,238,842 D475G possibly damaging Het
Numa1 C G 7: 102,000,665 probably null Het
Olfr807 T C 10: 129,754,955 E165G probably damaging Het
Prdm8 T C 5: 98,184,587 S116P possibly damaging Het
Pyroxd1 C G 6: 142,354,741 S199* probably null Het
R3hcc1l G T 19: 42,562,800 V79F probably benign Het
Rasal1 C T 5: 120,678,964 T779I probably benign Het
Scaper A C 9: 55,838,179 F596V probably damaging Het
Slco2b1 C T 7: 99,667,007 V439M probably benign Het
Slco6c1 A T 1: 97,062,424 D680E probably benign Het
Smurf1 A T 5: 144,879,369 F725L probably damaging Het
Snd1 A G 6: 28,880,249 E674G probably benign Het
Srcap C T 7: 127,541,776 P1515L probably damaging Het
Sycp2l A G 13: 41,143,465 M341V probably benign Het
Tmprss6 T A 15: 78,445,356 I492F probably damaging Het
Trip10 G T 17: 57,261,658 E416* probably null Het
Ugt1a5 A T 1: 88,166,425 N125I possibly damaging Het
Vmn2r7 G T 3: 64,716,404 S165Y probably damaging Het
Zfp663 A T 2: 165,353,005 H431Q probably benign Het
Other mutations in Tstd2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00232:Tstd2 APN 4 46120002 unclassified probably benign
IGL02832:Tstd2 APN 4 46124949 missense probably damaging 1.00
curative UTSW 4 46116960 missense probably damaging 1.00
R1991:Tstd2 UTSW 4 46120563 missense probably benign 0.00
R2154:Tstd2 UTSW 4 46129235 missense probably damaging 0.98
R4381:Tstd2 UTSW 4 46119933 missense probably benign 0.01
R4961:Tstd2 UTSW 4 46120467 missense probably damaging 1.00
R5282:Tstd2 UTSW 4 46120461 missense probably damaging 1.00
R5396:Tstd2 UTSW 4 46135542 missense probably benign 0.00
R7196:Tstd2 UTSW 4 46119955 missense probably damaging 1.00
R7535:Tstd2 UTSW 4 46116960 missense probably damaging 1.00
R7991:Tstd2 UTSW 4 46133646 missense unknown
Predicted Primers PCR Primer
(F):5'- GGGTTTCCCTCCTTTCGAGAAC -3'
(R):5'- GAACTGTAGCTCCTTGTGGCTC -3'

Sequencing Primer
(F):5'- TTTCGAGAACCCCCATGTGAAG -3'
(R):5'- ACTGGGAACTGTGACTCCCTG -3'
Posted On2015-10-08