Mutagenetix > Incidental Mutation

Incidental Mutation 'R4643:Mrgprf'

351737

Institutional Source

Beutler Lab

Gene Symbol

Mrgprf

Ensembl Gene

ENSMUSG00000031070

Gene Name

MAS-related GPR, member F

Synonyms

MrgF

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R4643 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

144854646-144863294 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 144862242 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Glutamine at position 268 (P268Q)

Ref Sequence

ENSEMBL: ENSMUSP00000033386 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033386] [ENSMUST00000117718]

AlphaFold

Q8VCJ6

Predicted Effect

probably benign
Transcript: ENSMUST00000033386
AA Change: P268Q

PolyPhen 2 Score 0.389 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000033386
Gene: ENSMUSG00000031070
AA Change: P268Q

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	52	189	4.5e-7	PFAM
Pfam:7tm_1	61	291	2.4e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000117718

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4833420G17Rik	A	G	13: 119,611,396 (GRCm39)	N398D	probably damaging	Het
Adamtsl4	G	A	3: 95,591,929 (GRCm39)	A58V	possibly damaging	Het
Anapc2	T	A	2: 25,166,406 (GRCm39)	V105E	probably benign	Het
C3ar1	A	T	6: 122,827,933 (GRCm39)	C95S	probably damaging	Het
Ccdc87	G	A	19: 4,891,877 (GRCm39)	G790R	probably damaging	Het
Cenpf	A	T	1: 189,391,786 (GRCm39)	M682K	probably benign	Het
Cyp2j13	T	C	4: 95,945,161 (GRCm39)	Q289R	possibly damaging	Het
Dclk2	A	T	3: 86,713,487 (GRCm39)	M453K	possibly damaging	Het
Dscam	G	A	16: 96,486,501 (GRCm39)	T1058M	probably damaging	Het
Gas2l3	CACTCGTCATACT	CACT	10: 89,266,820 (GRCm39)		probably benign	Het
Grip1	A	G	10: 119,856,006 (GRCm39)	N659S	probably damaging	Het
Hectd4	A	G	5: 121,487,118 (GRCm39)	K3371R	possibly damaging	Het
Il1a	T	A	2: 129,146,623 (GRCm39)	T157S	probably benign	Het
Il23r	A	G	6: 67,400,977 (GRCm39)	V451A	probably benign	Het
Iqch	T	C	9: 63,502,084 (GRCm39)	T40A	probably benign	Het
Irag2	A	T	6: 145,113,786 (GRCm39)	D318V	probably benign	Het
Kazald1	G	T	19: 45,066,788 (GRCm39)	V196L	probably benign	Het
L1td1	T	C	4: 98,626,120 (GRCm39)	S772P	probably damaging	Het
Lgi1	A	T	19: 38,289,158 (GRCm39)	D145V	probably damaging	Het
Lrp12	A	T	15: 39,735,418 (GRCm39)	L838Q	probably damaging	Het
Myef2	T	C	2: 124,958,731 (GRCm39)	K66R	possibly damaging	Het
Myo3b	A	G	2: 70,069,186 (GRCm39)	D475G	possibly damaging	Het
Numa1	C	G	7: 101,649,872 (GRCm39)		probably null	Het
Or6c214	T	C	10: 129,590,824 (GRCm39)	E165G	probably damaging	Het
Prdm8	T	C	5: 98,332,446 (GRCm39)	S116P	possibly damaging	Het
Pyroxd1	C	G	6: 142,300,467 (GRCm39)	S199*	probably null	Het
R3hcc1l	G	T	19: 42,551,239 (GRCm39)	V79F	probably benign	Het
Rasal1	C	T	5: 120,817,029 (GRCm39)	T779I	probably benign	Het
Scaper	A	C	9: 55,745,463 (GRCm39)	F596V	probably damaging	Het
Slco2b1	C	T	7: 99,316,214 (GRCm39)	V439M	probably benign	Het
Slco6c1	A	T	1: 96,990,149 (GRCm39)	D680E	probably benign	Het
Smurf1	A	T	5: 144,816,179 (GRCm39)	F725L	probably damaging	Het
Snd1	A	G	6: 28,880,248 (GRCm39)	E674G	probably benign	Het
Srcap	C	T	7: 127,140,948 (GRCm39)	P1515L	probably damaging	Het
Sycp2l	A	G	13: 41,296,941 (GRCm39)	M341V	probably benign	Het
Tmprss6	T	A	15: 78,329,556 (GRCm39)	I492F	probably damaging	Het
Trip10	G	T	17: 57,568,658 (GRCm39)	E416*	probably null	Het
Tstd2	T	C	4: 46,129,297 (GRCm39)	D177G	possibly damaging	Het
Ugt1a5	A	T	1: 88,094,147 (GRCm39)	N125I	possibly damaging	Het
Vmn2r7	G	T	3: 64,623,825 (GRCm39)	S165Y	probably damaging	Het
Zfp663	A	T	2: 165,194,925 (GRCm39)	H431Q	probably benign	Het

Other mutations in Mrgprf

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0973:Mrgprf	UTSW	7	144,861,993 (GRCm39)	missense	probably damaging	0.96
R1709:Mrgprf	UTSW	7	144,861,954 (GRCm39)	missense	probably benign	0.07
R5631:Mrgprf	UTSW	7	144,862,283 (GRCm39)	missense	probably benign	0.02
R5965:Mrgprf	UTSW	7	144,861,168 (GRCm39)	intron	probably benign
R7147:Mrgprf	UTSW	7	144,862,128 (GRCm39)	missense	possibly damaging	0.59
R7291:Mrgprf	UTSW	7	144,861,206 (GRCm39)	missense	unknown
R7755:Mrgprf	UTSW	7	144,862,380 (GRCm39)	missense	probably damaging	1.00
R8826:Mrgprf	UTSW	7	144,862,046 (GRCm39)	missense	probably benign	0.16
R8881:Mrgprf	UTSW	7	144,861,999 (GRCm39)	missense	probably benign
R9112:Mrgprf	UTSW	7	144,861,503 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TCTGCCGCAACATGGACATC -3'
(R):5'- CATTTCCATGGTGACCGTGTTG -3'

Sequencing Primer
(F):5'- AACATGGACATCGCCCTGG -3'
(R):5'- GACCGTGTTGGGTGTGC -3'

Posted On

2015-10-08