Mutagenetix > Incidental Mutation

Incidental Mutation 'R4666:Vcan'

351963

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, DPEAAE, heart defect, Cspg2, 5430420N07Rik

MMRRC Submission

041924-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4666 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

89655312-89742509 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 89679934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Leucine at position 2171 (W2171L)

Ref Sequence

ENSEMBL: ENSMUSP00000125446 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000109543
AA Change: W432L

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614
AA Change: W432L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109544
AA Change: W1392L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614
AA Change: W1392L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109546
AA Change: W3131L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: W3131L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000159285

SMART Domains

Protein: ENSMUSP00000125674
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
CLECT	3	85	3.48e-1	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000159910
AA Change: W2171L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614
AA Change: W2171L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000160740

SMART Domains

Protein: ENSMUSP00000125694
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
Pfam:Lectin_C	2	62	7.9e-7	PFAM
CCP	67	123	1.04e-8	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410137M14Rik	G	A	17: 36,978,902 (GRCm38)	S12L	probably benign	Het
4921507P07Rik	G	T	6: 50,595,828 (GRCm38)	T35K	possibly damaging	Het
4931406P16Rik	T	C	7: 34,284,773 (GRCm38)	M142V	probably damaging	Het
Abce1	A	G	8: 79,687,486 (GRCm38)	V532A	probably damaging	Het
Adamts12	T	A	15: 11,311,492 (GRCm38)	N1278K	probably benign	Het
Adipor1	T	A	1: 134,424,905 (GRCm38)	I138N	probably damaging	Het
Aox2	C	T	1: 58,304,597 (GRCm38)	Q480*	probably null	Het
Arhgef38	C	T	3: 133,140,772 (GRCm38)		probably null	Het
Atmin	A	G	8: 116,957,959 (GRCm38)	D786G	probably damaging	Het
Capn1	A	T	19: 6,011,015 (GRCm38)	N253K	probably benign	Het
Cdh8	T	C	8: 99,024,902 (GRCm38)	T728A	possibly damaging	Het
Celsr1	A	G	15: 86,030,494 (GRCm38)	S1093P	probably damaging	Het
Cep135	C	A	5: 76,616,854 (GRCm38)	P560T	probably benign	Het
Chfr	C	T	5: 110,144,867 (GRCm38)	Q167*	probably null	Het
Chrna4	A	G	2: 181,037,493 (GRCm38)	S54P	probably damaging	Het
Cntln	A	G	4: 84,971,216 (GRCm38)	N312S	probably benign	Het
Cntn6	A	G	6: 104,728,284 (GRCm38)	E154G	probably benign	Het
Col6a6	T	A	9: 105,767,342 (GRCm38)	Y1249F	possibly damaging	Het
Cpsf2	T	C	12: 101,983,207 (GRCm38)	S61P	probably damaging	Het
Cpvl	C	T	6: 53,931,933 (GRCm38)	E282K	probably benign	Het
Cryba2	C	T	1: 74,890,048 (GRCm38)	D179N	probably benign	Het
Daglb	A	T	5: 143,503,349 (GRCm38)	R654W	probably damaging	Het
Dennd3	A	G	15: 73,570,860 (GRCm38)	D1244G	probably damaging	Het
Dhx57	T	C	17: 80,274,961 (GRCm38)	E405G	probably damaging	Het
Dnah10	T	C	5: 124,828,472 (GRCm38)	M4060T	possibly damaging	Het
Dph1	A	G	11: 75,181,330 (GRCm38)	S238P	probably damaging	Het
Duox1	C	T	2: 122,319,475 (GRCm38)	P116S	probably benign	Het
Ebf1	A	G	11: 44,991,557 (GRCm38)	N447D	probably damaging	Het
Epg5	A	G	18: 78,012,864 (GRCm38)	N1751S	probably benign	Het
Exoc6	A	G	19: 37,570,505 (GRCm38)	D75G	probably damaging	Het
Extl2	T	A	3: 116,024,207 (GRCm38)	I70N	probably damaging	Het
Fam129c	G	T	8: 71,603,825 (GRCm38)	E390*	probably null	Het
Fanca	T	C	8: 123,268,972 (GRCm38)	T1364A	probably damaging	Het
Fbln7	T	A	2: 128,894,910 (GRCm38)		probably null	Het
Foxa3	G	T	7: 19,014,372 (GRCm38)	C275*	probably null	Het
Foxred1	C	T	9: 35,210,855 (GRCm38)		probably benign	Het
Galr2	A	G	11: 116,283,629 (GRCm38)	T362A	probably benign	Het
Garem2	C	A	5: 30,114,667 (GRCm38)	R376S	probably damaging	Het
Gatc	T	A	5: 115,335,547 (GRCm38)	N111I	probably benign	Het
Gjb4	C	A	4: 127,351,778 (GRCm38)	K123N	probably damaging	Het
Gm14025	T	C	2: 129,038,230 (GRCm38)	H592R	probably benign	Het
Gm9894	T	C	13: 67,765,094 (GRCm38)		noncoding transcript	Het
Gtdc1	T	C	2: 44,591,925 (GRCm38)	N301S	probably benign	Het
Gtf2ird1	T	A	5: 134,383,902 (GRCm38)	E55V	probably damaging	Het
Gtsf1	T	C	15: 103,421,205 (GRCm38)	I96V	probably benign	Het
Homer1	T	A	13: 93,402,159 (GRCm38)	I170N	probably damaging	Het
Homer3	G	A	8: 70,290,143 (GRCm38)		probably null	Het
Hoxb9	A	G	11: 96,274,831 (GRCm38)	K242R	possibly damaging	Het
Ifna14	T	C	4: 88,571,336 (GRCm38)	R155G	probably benign	Het
Lrrc7	GAAGTTGTTTGGAGATTCTTATCTTA	GA	3: 158,318,408 (GRCm38)		probably benign	Het
Lsm11	A	G	11: 45,933,813 (GRCm38)	S296P	probably damaging	Het
Macrod2	T	C	2: 142,217,599 (GRCm38)	L265P	probably damaging	Het
Mcu	G	A	10: 59,456,699 (GRCm38)	L53F	probably damaging	Het
Mpv17l2	A	G	8: 70,760,415 (GRCm38)	V104A	possibly damaging	Het
Myh10	G	A	11: 68,801,730 (GRCm38)		probably null	Het
Nemf	T	C	12: 69,312,280 (GRCm38)	E1031G	probably damaging	Het
Nhsl1	G	A	10: 18,531,405 (GRCm38)	S1395N	probably damaging	Het
Nlrp2	T	A	7: 5,319,189 (GRCm38)	I82F	probably benign	Het
Nlrp4e	T	A	7: 23,336,780 (GRCm38)	L686*	probably null	Het
Nudt12os	T	A	17: 59,024,551 (GRCm38)		noncoding transcript	Het
Olfr1122	T	A	2: 87,387,876 (GRCm38)	I57K	probably damaging	Het
Olfr16	G	A	1: 172,957,590 (GRCm38)	S265N	probably benign	Het
Olfr180	T	C	16: 58,916,584 (GRCm38)	D19G	probably benign	Het
Olfr205	A	G	16: 59,329,210 (GRCm38)	Y100H	possibly damaging	Het
Olfr93	A	T	17: 37,151,379 (GRCm38)	S44T	possibly damaging	Het
Olfr944	T	A	9: 39,217,846 (GRCm38)	M163K	probably damaging	Het
Pde7a	T	C	3: 19,260,256 (GRCm38)	T59A	probably damaging	Het
Pde7b	A	C	10: 20,438,750 (GRCm38)	D203E	probably damaging	Het
Phkg2	T	A	7: 127,577,984 (GRCm38)	I94N	possibly damaging	Het
Pik3r2	G	A	8: 70,768,859 (GRCm38)	T667I	possibly damaging	Het
Pitx3	T	A	19: 46,137,101 (GRCm38)	H68L	possibly damaging	Het
Prcd	A	G	11: 116,668,164 (GRCm38)		probably benign	Het
Prune2	C	T	19: 17,120,188 (GRCm38)	R1019*	probably null	Het
Psap	A	G	10: 60,300,545 (GRCm38)	D486G	probably benign	Het
Purb	A	T	11: 6,475,615 (GRCm38)	V91E	probably damaging	Het
Recql	C	A	6: 142,376,841 (GRCm38)	V112F	probably damaging	Het
Rptor	A	T	11: 119,743,882 (GRCm38)	I175F	probably damaging	Het
Sbf1	C	T	15: 89,295,246 (GRCm38)	V1385M	probably damaging	Het
Serpinb13	C	T	1: 106,982,844 (GRCm38)	S66L	probably damaging	Het
Slc35f6	T	C	5: 30,655,613 (GRCm38)	L37P	probably damaging	Het
Slc6a3	T	A	13: 73,538,581 (GRCm38)	N22K	possibly damaging	Het
Sorl1	A	T	9: 42,004,051 (GRCm38)	M1294K	probably damaging	Het
Sp6	C	A	11: 97,021,875 (GRCm38)	A138E	probably benign	Het
Spag8	G	T	4: 43,653,408 (GRCm38)		probably benign	Het
Spon1	T	A	7: 114,028,969 (GRCm38)	M320K	probably benign	Het
Tceanc2	A	T	4: 107,165,560 (GRCm38)	S77T	probably damaging	Het
Thsd7a	T	A	6: 12,504,013 (GRCm38)	I381F	possibly damaging	Het
Thsd7a	T	A	6: 12,337,314 (GRCm38)	T1235S	possibly damaging	Het
Tmc4	T	C	7: 3,671,271 (GRCm38)		probably null	Het
Tmprss11d	T	C	5: 86,309,401 (GRCm38)	D133G	probably damaging	Het
Trav13n-3	T	A	14: 53,337,496 (GRCm38)	V65D	probably damaging	Het
Trpm1	G	T	7: 64,203,034 (GRCm38)	L65F	probably damaging	Het
Tyk2	C	T	9: 21,114,207 (GRCm38)	A741T	probably damaging	Het
Ube2v1	T	A	2: 167,610,377 (GRCm38)	Y102F	probably damaging	Het
Uckl1	A	T	2: 181,574,868 (GRCm38)	S95T	possibly damaging	Het
Uhrf1bp1	G	T	17: 27,893,503 (GRCm38)	W1222L	possibly damaging	Het
Vmn1r64	T	C	7: 5,884,358 (GRCm38)	N62S	probably damaging	Het
Vmn2r67	T	C	7: 85,150,623 (GRCm38)	D469G	probably benign	Het
Vps13b	A	G	15: 35,640,544 (GRCm38)	S1352G	probably benign	Het
Zbtb38	C	T	9: 96,688,383 (GRCm38)	R216H	probably damaging	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89,704,702 (GRCm38)	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89,692,319 (GRCm38)	missense	probably benign
IGL00504:Vcan	APN	13	89,691,275 (GRCm38)	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89,688,979 (GRCm38)	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89,703,726 (GRCm38)	missense	probably benign
IGL00743:Vcan	APN	13	89,725,306 (GRCm38)	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89,662,052 (GRCm38)	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89,679,958 (GRCm38)	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89,691,668 (GRCm38)	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89,703,943 (GRCm38)	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89,704,169 (GRCm38)	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89,688,675 (GRCm38)	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89,690,586 (GRCm38)	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89,689,745 (GRCm38)	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89,725,388 (GRCm38)	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89,689,205 (GRCm38)	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89,689,359 (GRCm38)	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89,684,438 (GRCm38)	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89,725,565 (GRCm38)	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89,684,493 (GRCm38)	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89,703,077 (GRCm38)	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89,704,849 (GRCm38)	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89,690,657 (GRCm38)	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89,688,623 (GRCm38)	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89,703,275 (GRCm38)	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89,661,932 (GRCm38)	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89,705,006 (GRCm38)	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89,661,985 (GRCm38)	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89,703,668 (GRCm38)	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89,703,668 (GRCm38)	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89,678,073 (GRCm38)	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89,691,261 (GRCm38)	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89,712,191 (GRCm38)	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89,691,275 (GRCm38)	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89,703,546 (GRCm38)	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89,703,199 (GRCm38)	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89,678,145 (GRCm38)	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89,704,660 (GRCm38)	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89,703,772 (GRCm38)	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89,731,464 (GRCm38)	missense	possibly damaging	0.86
R0561:Vcan	UTSW	13	89,712,253 (GRCm38)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,712,267 (GRCm38)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,704,706 (GRCm38)	missense	probably damaging	0.99
R0680:Vcan	UTSW	13	89,679,822 (GRCm38)	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89,704,953 (GRCm38)	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89,685,077 (GRCm38)	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89,692,410 (GRCm38)	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89,705,663 (GRCm38)	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89,704,303 (GRCm38)	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89,679,794 (GRCm38)	splice site	probably null
R1219:Vcan	UTSW	13	89,679,904 (GRCm38)	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89,657,556 (GRCm38)	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89,693,055 (GRCm38)	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89,693,055 (GRCm38)	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89,688,484 (GRCm38)	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89,688,484 (GRCm38)	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89,692,956 (GRCm38)	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89,689,661 (GRCm38)	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89,705,663 (GRCm38)	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89,703,667 (GRCm38)	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89,661,946 (GRCm38)	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89,703,547 (GRCm38)	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89,688,483 (GRCm38)	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89,721,775 (GRCm38)	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89,704,735 (GRCm38)	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89,691,681 (GRCm38)	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89,705,212 (GRCm38)	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89,705,392 (GRCm38)	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89,690,871 (GRCm38)	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89,693,015 (GRCm38)	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89,705,534 (GRCm38)	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89,702,926 (GRCm38)	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89,703,424 (GRCm38)	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89,692,742 (GRCm38)	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89,689,038 (GRCm38)	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89,690,985 (GRCm38)	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89,689,737 (GRCm38)	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89,703,529 (GRCm38)	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89,703,410 (GRCm38)	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89,689,449 (GRCm38)	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89,704,675 (GRCm38)	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89,704,237 (GRCm38)	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89,703,301 (GRCm38)	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89,679,887 (GRCm38)	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89,685,158 (GRCm38)	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89,725,547 (GRCm38)	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89,725,486 (GRCm38)	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89,704,028 (GRCm38)	missense	probably damaging	0.97
R4785:Vcan	UTSW	13	89,705,789 (GRCm38)	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89,704,739 (GRCm38)	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89,688,842 (GRCm38)	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89,703,977 (GRCm38)	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89,657,472 (GRCm38)	intron	probably benign
R5124:Vcan	UTSW	13	89,725,517 (GRCm38)	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89,690,240 (GRCm38)	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89,690,872 (GRCm38)	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89,692,532 (GRCm38)	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89,691,600 (GRCm38)	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89,690,286 (GRCm38)	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89,691,810 (GRCm38)	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89,703,112 (GRCm38)	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89,688,671 (GRCm38)	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89,691,503 (GRCm38)	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89,691,135 (GRCm38)	frame shift	probably null
R5687:Vcan	UTSW	13	89,678,134 (GRCm38)	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89,679,950 (GRCm38)	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89,703,952 (GRCm38)	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89,692,691 (GRCm38)	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89,657,536 (GRCm38)	nonsense	probably null
R6135:Vcan	UTSW	13	89,689,926 (GRCm38)	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89,691,220 (GRCm38)	nonsense	probably null
R6280:Vcan	UTSW	13	89,725,373 (GRCm38)	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89,691,597 (GRCm38)	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89,704,832 (GRCm38)	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89,690,687 (GRCm38)	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89,704,731 (GRCm38)	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89,705,182 (GRCm38)	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89,705,125 (GRCm38)	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89,712,381 (GRCm38)	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89,689,431 (GRCm38)	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89,678,133 (GRCm38)	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89,679,956 (GRCm38)	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89,693,407 (GRCm38)	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89,690,618 (GRCm38)	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89,690,241 (GRCm38)	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89,705,686 (GRCm38)	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89,689,110 (GRCm38)	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89,725,591 (GRCm38)	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89,688,936 (GRCm38)	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89,705,270 (GRCm38)	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89,731,457 (GRCm38)	critical splice donor site	probably null
R7250:Vcan	UTSW	13	89,721,686 (GRCm38)	missense	probably damaging	1.00
R7262:Vcan	UTSW	13	89,705,161 (GRCm38)	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89,692,733 (GRCm38)	nonsense	probably null
R7299:Vcan	UTSW	13	89,705,266 (GRCm38)	missense	probably benign
R7301:Vcan	UTSW	13	89,705,266 (GRCm38)	missense	probably benign
R7425:Vcan	UTSW	13	89,689,832 (GRCm38)	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89,704,118 (GRCm38)	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89,692,458 (GRCm38)	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89,692,223 (GRCm38)	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89,685,114 (GRCm38)	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89,685,114 (GRCm38)	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89,691,789 (GRCm38)	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89,704,619 (GRCm38)	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89,689,323 (GRCm38)	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89,692,937 (GRCm38)	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89,688,654 (GRCm38)	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89,688,654 (GRCm38)	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89,691,858 (GRCm38)	missense	probably benign
R7998:Vcan	UTSW	13	89,704,327 (GRCm38)	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89,704,360 (GRCm38)	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89,657,290 (GRCm38)	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89,703,320 (GRCm38)	nonsense	probably null
R8103:Vcan	UTSW	13	89,657,658 (GRCm38)	missense	probably damaging	1.00
R8124:Vcan	UTSW	13	89,704,254 (GRCm38)	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89,704,987 (GRCm38)	nonsense	probably null
R8166:Vcan	UTSW	13	89,692,736 (GRCm38)	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89,704,970 (GRCm38)	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89,704,335 (GRCm38)	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89,688,743 (GRCm38)	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89,691,098 (GRCm38)	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89,692,320 (GRCm38)	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89,692,111 (GRCm38)	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89,704,907 (GRCm38)	missense	probably benign
R9049:Vcan	UTSW	13	89,678,105 (GRCm38)	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89,691,027 (GRCm38)	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89,704,525 (GRCm38)	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89,679,931 (GRCm38)	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89,690,496 (GRCm38)	nonsense	probably null
R9259:Vcan	UTSW	13	89,690,870 (GRCm38)	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89,689,333 (GRCm38)	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89,703,412 (GRCm38)	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89,693,009 (GRCm38)	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89,691,540 (GRCm38)	missense
R9579:Vcan	UTSW	13	89,689,594 (GRCm38)	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89,705,372 (GRCm38)	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89,692,962 (GRCm38)	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89,691,741 (GRCm38)	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89,691,128 (GRCm38)	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89,689,811 (GRCm38)	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89,692,493 (GRCm38)	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89,705,749 (GRCm38)	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89,692,571 (GRCm38)	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89,704,073 (GRCm38)	missense	probably damaging	1.00
Z1177:Vcan	UTSW	13	89,703,788 (GRCm38)	nonsense	probably null
Z1177:Vcan	UTSW	13	89,703,524 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGGGTTCCAAAGAAAGCTACG -3'
(R):5'- GGATCAGTTGGCTTACTTTACAATG -3'

Sequencing Primer
(F):5'- GTTCCAAAGAAAGCTACGATAGTG -3'
(R):5'- GTTGTTTTTGCCACTGTGATCAAAAC -3'

Posted On

2015-10-08