Mutagenetix > Incidental Mutation

Incidental Mutation 'R4667:F5'

351993

Institutional Source

Beutler Lab

Gene Symbol

Ensembl Gene

ENSMUSG00000026579

Gene Name

coagulation factor V

Synonyms

Cf-5, Cf5

MMRRC Submission

042012-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4667 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

164151838-164220277 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 164174186 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 153 (V153I)

Ref Sequence

ENSEMBL: ENSMUSP00000083204 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086040]

AlphaFold

O88783

Predicted Effect

probably benign
Transcript: ENSMUST00000086040
AA Change: V153I

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000083204
Gene: ENSMUSG00000026579
AA Change: V153I

Domain	Start	End	E-Value	Type
low complexity region	2	14	N/A	INTRINSIC
Pfam:Cu-oxidase_3	67	196	4.4e-10	PFAM
low complexity region	282	300	N/A	INTRINSIC
Pfam:Cu-oxidase_3	397	527	1.5e-7	PFAM
low complexity region	1013	1019	N/A	INTRINSIC
low complexity region	1045	1058	N/A	INTRINSIC
low complexity region	1156	1173	N/A	INTRINSIC
low complexity region	1352	1366	N/A	INTRINSIC
low complexity region	1368	1382	N/A	INTRINSIC
low complexity region	1440	1464	N/A	INTRINSIC
Pfam:Cu-oxidase_3	1600	1714	9.1e-8	PFAM
FA58C	1865	2020	8.03e-36	SMART
FA58C	2024	2180	1.96e-30	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a glycoprotein coagulation factor that plays a critical role in the process of blood coagulation and hemostasis. The encoded protein is activated by thrombin, to generate a heterodimer containing heavy and light chains held together by calcium ions. About half of the mice lacking the encoded protein die at an embryonic stage possible due to abnormal yolk-sac vasculature while the remaining animals succumbed to massive hemorrhage immediately after birth. A point mutation in this gene has been shown to cause disseminated intravascular thrombosis in the perinatal period, resulting in frequent deaths of newborn mice. [provided by RefSeq, Apr 2015]
PHENOTYPE: Half of mice homozygous for a null allele die at E9-E10 with defects in yolk-sac vasculature and somite formation; the remaining half develop to term but die of massive hemorrhage within hours of birth. Mice homozygous for a knock-in (F5 Leiden) allele develop strain-specific perinatal thrombosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 109 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad8	A	G	9: 26,990,627 (GRCm38)	L147P	probably damaging	Het
Adgra3	T	A	5: 49,978,956 (GRCm38)	Y729F	possibly damaging	Het
Ago2	A	G	15: 73,146,416 (GRCm38)	Y58H	probably damaging	Het
Akap13	A	G	7: 75,729,094 (GRCm38)	T2128A	probably damaging	Het
Akap2	A	T	4: 57,855,655 (GRCm38)	D328V	possibly damaging	Het
Ankhd1	C	A	18: 36,648,021 (GRCm38)	P2042Q	possibly damaging	Het
Arhgef15	T	C	11: 68,954,561 (GRCm38)	K155R	probably benign	Het
Atp10b	T	C	11: 43,247,518 (GRCm38)	F1209L	probably damaging	Het
B130006D01Rik	A	T	11: 95,726,509 (GRCm38)		probably benign	Het
Bmpr2	T	C	1: 59,867,716 (GRCm38)	L656S	probably damaging	Het
Bnip5	G	T	17: 28,908,313 (GRCm38)	Q241K	possibly damaging	Het
Btbd17	A	G	11: 114,793,857 (GRCm38)	F119L	possibly damaging	Het
Ccdc191	G	T	16: 43,931,283 (GRCm38)	K267N	probably damaging	Het
Cd200l1	A	G	16: 45,444,163 (GRCm38)	S11P	probably benign	Het
Ceacam20	T	C	7: 19,986,027 (GRCm38)	Y495H	probably damaging	Het
Celf2	T	C	2: 6,721,528 (GRCm38)	I47V	probably benign	Het
Cemip2	C	T	19: 21,844,781 (GRCm38)	A1180V	probably benign	Het
Cemip2	G	A	19: 21,797,351 (GRCm38)	R119H	probably benign	Het
Chd9	T	C	8: 91,033,800 (GRCm38)	S2058P	possibly damaging	Het
Clcn6	T	C	4: 148,024,167 (GRCm38)	E135G	possibly damaging	Het
Cntn1	T	A	15: 92,295,079 (GRCm38)	N687K	probably damaging	Het
Col1a2	A	T	6: 4,512,412 (GRCm38)	M99L	unknown	Het
Cpeb2	T	C	5: 43,233,892 (GRCm38)		probably benign	Het
Csn1s2b	A	G	5: 87,822,311 (GRCm38)	T134A	possibly damaging	Het
Cst13	A	T	2: 148,823,081 (GRCm38)		probably benign	Het
Cyp2c66	T	A	19: 39,176,656 (GRCm38)	D360E	probably damaging	Het
Dhx8	A	G	11: 101,738,161 (GRCm38)	S179G	unknown	Het
Dip2b	A	G	15: 100,151,360 (GRCm38)	I212V	probably benign	Het
Dnah9	G	A	11: 66,155,531 (GRCm38)	H64Y	probably benign	Het
Dnal1	T	C	12: 84,136,700 (GRCm38)		probably benign	Het
Dse	T	G	10: 34,153,012 (GRCm38)	Y694S	probably damaging	Het
Dync2h1	T	C	9: 7,051,411 (GRCm38)	I3175V	probably benign	Het
Elf5	A	G	2: 103,449,060 (GRCm38)	N209D	probably damaging	Het
Elovl1	A	G	4: 118,430,787 (GRCm38)	Y40C	probably damaging	Het
Erp27	T	C	6: 136,908,152 (GRCm38)	E216G	possibly damaging	Het
Fam186a	G	A	15: 99,944,532 (GRCm38)	T1277I	possibly damaging	Het
Fam90a1a	A	T	8: 21,963,346 (GRCm38)	H239L	possibly damaging	Het
Fchsd2	G	T	7: 101,250,449 (GRCm38)	R334L	probably damaging	Het
Fermt3	T	C	19: 7,002,920 (GRCm38)	Y369C	probably damaging	Het
Fhod3	C	T	18: 25,066,338 (GRCm38)	P689S	probably benign	Het
Fnbp1l	G	T	3: 122,556,567 (GRCm38)	Q332K	probably benign	Het
Frem3	T	C	8: 80,663,420 (GRCm38)	S1767P	probably damaging	Het
Ggt5	T	C	10: 75,603,031 (GRCm38)	L121P	probably damaging	Het
Gphn	T	C	12: 78,454,817 (GRCm38)	S119P	probably damaging	Het
Herc2	A	G	7: 56,131,253 (GRCm38)	D1222G	probably damaging	Het
Hmx3	T	C	7: 131,544,382 (GRCm38)	I273T	possibly damaging	Het
Hnrnpu	A	G	1: 178,332,181 (GRCm38)		probably benign	Het
Hspg2	C	T	4: 137,539,645 (GRCm38)	T1987I	possibly damaging	Het
Ighv1-22	T	A	12: 114,746,451 (GRCm38)	Q58L	probably damaging	Het
Ighv14-3	T	A	12: 114,060,255 (GRCm38)	I7F	probably benign	Het
Kcns3	C	A	12: 11,091,783 (GRCm38)	R305L	probably damaging	Het
Kcnu1	C	T	8: 25,910,921 (GRCm38)	A699V	possibly damaging	Het
Kif22	A	C	7: 127,033,328 (GRCm38)	L270W	probably damaging	Het
Lrp2	G	T	2: 69,489,298 (GRCm38)	H1960Q	probably benign	Het
Marchf7	C	T	2: 60,241,050 (GRCm38)	Q94*	probably null	Het
Mcoln3	A	T	3: 146,131,204 (GRCm38)	I264F	probably benign	Het
Mdn1	A	C	4: 32,679,572 (GRCm38)	T706P	probably damaging	Het
Mfsd2b	A	G	12: 4,867,636 (GRCm38)	C137R	probably benign	Het
Mmp25	A	G	17: 23,644,607 (GRCm38)	V83A	probably benign	Het
Mocos	T	C	18: 24,666,434 (GRCm38)	Y242H	probably benign	Het
Msh6	T	C	17: 87,984,806 (GRCm38)	S330P	possibly damaging	Het
Mtus2	T	C	5: 148,298,260 (GRCm38)	S1156P	possibly damaging	Het
Muc5b	G	A	7: 141,842,379 (GRCm38)	R124H	unknown	Het
Mybbp1a	G	A	11: 72,447,971 (GRCm38)	E775K	possibly damaging	Het
Myo10	A	G	15: 25,793,153 (GRCm38)	E1272G	possibly damaging	Het
Nars1	A	G	18: 64,505,231 (GRCm38)	S254P	possibly damaging	Het
Ncapd2	A	G	6: 125,184,518 (GRCm38)	I211T	possibly damaging	Het
Ncoa7	A	T	10: 30,690,790 (GRCm38)	W582R	probably damaging	Het
Npr3	T	A	15: 11,905,467 (GRCm38)	D58V	possibly damaging	Het
Nr3c1	G	T	18: 39,428,727 (GRCm38)	T430K	probably benign	Het
Odf2l	A	G	3: 145,128,040 (GRCm38)	T111A	probably benign	Het
Ogdh	G	T	11: 6,340,600 (GRCm38)	C406F	probably benign	Het
Olfml2a	T	G	2: 38,949,010 (GRCm38)	S190A	probably damaging	Het
Optn	T	C	2: 5,033,139 (GRCm38)	K415E	probably benign	Het
Or10n1	T	C	9: 39,613,738 (GRCm38)	M57T	probably damaging	Het
Or52a20	A	G	7: 103,716,638 (GRCm38)	T15A	probably benign	Het
Or8b12i	T	C	9: 20,171,098 (GRCm38)	I158V	probably benign	Het
Or8g52	G	T	9: 39,719,709 (GRCm38)	V161F	probably benign	Het
Perm1	C	A	4: 156,220,206 (GRCm38)	S803*	probably null	Het
Pex14	T	C	4: 148,984,085 (GRCm38)	T84A	probably benign	Het
Pih1d2	T	A	9: 50,620,952 (GRCm38)	Y103*	probably null	Het
Pikfyve	T	A	1: 65,250,273 (GRCm38)	C1235S	probably damaging	Het
Polr1a	A	G	6: 71,917,821 (GRCm38)	N171S	probably benign	Het
Prrx1	A	G	1: 163,254,047 (GRCm38)	S201P	probably benign	Het
Psme2b	A	T	11: 48,945,666 (GRCm38)	N151K	probably benign	Het
Serpinb5	A	T	1: 106,872,295 (GRCm38)	T72S	probably benign	Het
Sgsm1	A	G	5: 113,260,047 (GRCm38)		probably null	Het
Sipa1l2	T	C	8: 125,453,470 (GRCm38)	R1063G	possibly damaging	Het
Slc19a3	T	C	1: 83,022,799 (GRCm38)	T166A	probably benign	Het
Slc5a4b	T	C	10: 76,075,045 (GRCm38)	Y319C	possibly damaging	Het
Stard3nl	T	A	13: 19,376,519 (GRCm38)	N29Y	probably damaging	Het
Sult6b2	G	T	6: 142,801,695 (GRCm38)	C109*	probably null	Het
Tcf25	A	G	8: 123,397,025 (GRCm38)	E467G	possibly damaging	Het
Tmem177	A	T	1: 119,910,220 (GRCm38)	V243D	probably benign	Het
Top2b	T	G	14: 16,409,189 (GRCm38)	I777M	probably damaging	Het
Tspan11	T	A	6: 127,943,715 (GRCm38)	C208*	probably null	Het
Ttc1	A	G	11: 43,745,317 (GRCm38)	V33A	probably benign	Het
Tut4	G	A	4: 108,495,159 (GRCm38)	E357K	probably damaging	Het
Uck1	T	A	2: 32,256,034 (GRCm38)	H283L	probably damaging	Het
Utrn	A	C	10: 12,698,053 (GRCm38)	V1091G	probably benign	Het
Vmn1r11	A	T	6: 57,137,498 (GRCm38)	H49L	probably damaging	Het
Vmn1r160	G	T	7: 22,872,053 (GRCm38)	S277I	probably benign	Het
Vmn1r18	A	T	6: 57,390,084 (GRCm38)	S162T	probably benign	Het
Vps37b	A	G	5: 124,010,732 (GRCm38)	L80P	probably damaging	Het
Wfdc3	T	C	2: 164,743,086 (GRCm38)	M1V	probably null	Het
Wrn	A	T	8: 33,324,338 (GRCm38)	N116K	probably benign	Het
Wscd2	G	T	5: 113,577,272 (GRCm38)	G391V	probably damaging	Het
Zfp286	A	G	11: 62,780,602 (GRCm38)	V215A	probably benign	Het
Zfp568	A	G	7: 30,023,277 (GRCm38)	H549R	probably damaging	Het

Other mutations in F5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00840:F5	APN	1	164,179,524 (GRCm38)	missense	probably benign	0.15
IGL00843:F5	APN	1	164,211,791 (GRCm38)	missense	probably benign	0.00
IGL00904:F5	APN	1	164,194,009 (GRCm38)	missense	probably benign
IGL00913:F5	APN	1	164,204,896 (GRCm38)	missense	probably damaging	1.00
IGL01099:F5	APN	1	164,194,334 (GRCm38)	missense	probably damaging	0.99
IGL01134:F5	APN	1	164,191,979 (GRCm38)	missense	possibly damaging	0.87
IGL01313:F5	APN	1	164,193,612 (GRCm38)	missense	probably benign	0.01
IGL01635:F5	APN	1	164,207,858 (GRCm38)	missense	probably benign	0.00
IGL01697:F5	APN	1	164,194,052 (GRCm38)	missense	probably benign	0.04
IGL01768:F5	APN	1	164,176,345 (GRCm38)	missense	probably benign	0.22
IGL01795:F5	APN	1	164,194,390 (GRCm38)	missense	probably benign	0.00
IGL01835:F5	APN	1	164,194,368 (GRCm38)	missense	probably benign	0.12
IGL01843:F5	APN	1	164,211,826 (GRCm38)	missense	probably benign	0.05
IGL01989:F5	APN	1	164,176,307 (GRCm38)	missense	probably benign	0.39
IGL02036:F5	APN	1	164,183,002 (GRCm38)	splice site	probably benign
IGL02065:F5	APN	1	164,190,126 (GRCm38)	missense	probably damaging	1.00
IGL02077:F5	APN	1	164,198,866 (GRCm38)	missense	probably damaging	1.00
IGL02139:F5	APN	1	164,192,674 (GRCm38)	missense	possibly damaging	0.89
IGL02210:F5	APN	1	164,190,141 (GRCm38)	missense	probably benign	0.00
IGL02415:F5	APN	1	164,191,929 (GRCm38)	missense	probably damaging	1.00
IGL02440:F5	APN	1	164,207,066 (GRCm38)	missense	possibly damaging	0.79
IGL02471:F5	APN	1	164,174,291 (GRCm38)	missense	probably damaging	1.00
IGL02535:F5	APN	1	164,198,733 (GRCm38)	missense	probably damaging	0.98
IGL02537:F5	APN	1	164,193,117 (GRCm38)	missense	probably benign	0.26
IGL02628:F5	APN	1	164,194,075 (GRCm38)	missense	probably damaging	0.99
IGL02638:F5	APN	1	164,184,608 (GRCm38)	critical splice donor site	probably null
IGL02824:F5	APN	1	164,194,347 (GRCm38)	missense	probably benign	0.00
IGL02977:F5	APN	1	164,194,021 (GRCm38)	missense	probably damaging	1.00
IGL03028:F5	APN	1	164,193,000 (GRCm38)	nonsense	probably null
IGL03064:F5	APN	1	164,195,594 (GRCm38)	missense	probably benign	0.04
IGL03127:F5	APN	1	164,193,538 (GRCm38)	missense	probably benign	0.45
IGL03131:F5	APN	1	164,161,819 (GRCm38)	missense	possibly damaging	0.62
IGL03348:F5	APN	1	164,194,152 (GRCm38)	missense	possibly damaging	0.49
IGL03387:F5	APN	1	164,193,232 (GRCm38)	missense	probably damaging	1.00
James_dean	UTSW	1	164,204,820 (GRCm38)	missense	probably benign	0.43
BB002:F5	UTSW	1	164,176,366 (GRCm38)	critical splice donor site	probably null
BB012:F5	UTSW	1	164,176,366 (GRCm38)	critical splice donor site	probably null
R0002:F5	UTSW	1	164,201,631 (GRCm38)	missense	probably damaging	1.00
R0095:F5	UTSW	1	164,191,968 (GRCm38)	nonsense	probably null
R0116:F5	UTSW	1	164,184,914 (GRCm38)	missense	probably benign	0.01
R0359:F5	UTSW	1	164,179,449 (GRCm38)	missense	probably damaging	1.00
R0426:F5	UTSW	1	164,182,840 (GRCm38)	missense	probably damaging	0.99
R0452:F5	UTSW	1	164,185,107 (GRCm38)	missense	probably damaging	0.99
R0457:F5	UTSW	1	164,194,200 (GRCm38)	missense	probably benign	0.00
R0520:F5	UTSW	1	164,209,587 (GRCm38)	missense	probably benign	0.15
R0522:F5	UTSW	1	164,211,763 (GRCm38)	missense	probably damaging	1.00
R0554:F5	UTSW	1	164,179,449 (GRCm38)	missense	probably damaging	1.00
R0575:F5	UTSW	1	164,176,244 (GRCm38)	missense	probably damaging	1.00
R0734:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R0739:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1062:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1063:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1149:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1149:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1150:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1151:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1152:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1221:F5	UTSW	1	164,161,799 (GRCm38)	missense	probably damaging	1.00
R1284:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1286:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1358:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1360:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1362:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1383:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1465:F5	UTSW	1	164,198,833 (GRCm38)	missense	probably benign	0.02
R1465:F5	UTSW	1	164,198,833 (GRCm38)	missense	probably benign	0.02
R1545:F5	UTSW	1	164,208,960 (GRCm38)	nonsense	probably null
R1561:F5	UTSW	1	164,186,903 (GRCm38)	nonsense	probably null
R1623:F5	UTSW	1	164,195,622 (GRCm38)	missense	probably damaging	1.00
R1662:F5	UTSW	1	164,207,888 (GRCm38)	missense	probably damaging	1.00
R1673:F5	UTSW	1	164,179,520 (GRCm38)	missense	probably damaging	1.00
R1689:F5	UTSW	1	164,198,917 (GRCm38)	missense	probably damaging	1.00
R1705:F5	UTSW	1	164,217,490 (GRCm38)	missense	possibly damaging	0.92
R1732:F5	UTSW	1	164,174,150 (GRCm38)	missense	probably damaging	1.00
R1763:F5	UTSW	1	164,192,535 (GRCm38)	missense	probably benign	0.04
R1774:F5	UTSW	1	164,192,535 (GRCm38)	missense	probably benign	0.04
R1799:F5	UTSW	1	164,193,531 (GRCm38)	missense	possibly damaging	0.58
R1800:F5	UTSW	1	164,182,834 (GRCm38)	missense	probably damaging	1.00
R1842:F5	UTSW	1	164,184,560 (GRCm38)	missense	probably damaging	0.99
R1915:F5	UTSW	1	164,182,917 (GRCm38)	missense	probably damaging	0.97
R1926:F5	UTSW	1	164,179,508 (GRCm38)	missense	probably damaging	1.00
R2025:F5	UTSW	1	164,209,475 (GRCm38)	missense	probably benign	0.05
R2198:F5	UTSW	1	164,207,034 (GRCm38)	missense	probably damaging	1.00
R2258:F5	UTSW	1	164,192,181 (GRCm38)	missense	probably damaging	1.00
R2264:F5	UTSW	1	164,194,402 (GRCm38)	missense	probably benign	0.32
R2281:F5	UTSW	1	164,195,720 (GRCm38)	missense	possibly damaging	0.80
R2407:F5	UTSW	1	164,211,872 (GRCm38)	missense	probably damaging	1.00
R2445:F5	UTSW	1	164,190,226 (GRCm38)	missense	probably damaging	1.00
R2860:F5	UTSW	1	164,184,964 (GRCm38)	missense	probably damaging	1.00
R2861:F5	UTSW	1	164,184,964 (GRCm38)	missense	probably damaging	1.00
R2862:F5	UTSW	1	164,184,964 (GRCm38)	missense	probably damaging	1.00
R2899:F5	UTSW	1	164,186,900 (GRCm38)	missense	possibly damaging	0.88
R2910:F5	UTSW	1	164,204,820 (GRCm38)	missense	probably benign	0.43
R2912:F5	UTSW	1	164,193,919 (GRCm38)	missense	probably damaging	0.98
R2996:F5	UTSW	1	164,182,917 (GRCm38)	missense	probably damaging	0.97
R3745:F5	UTSW	1	164,186,779 (GRCm38)	missense	possibly damaging	0.79
R3901:F5	UTSW	1	164,176,229 (GRCm38)	missense	probably benign	0.08
R3902:F5	UTSW	1	164,176,229 (GRCm38)	missense	probably benign	0.08
R4365:F5	UTSW	1	164,184,950 (GRCm38)	missense	probably damaging	0.98
R4448:F5	UTSW	1	164,198,899 (GRCm38)	missense	possibly damaging	0.52
R4490:F5	UTSW	1	164,217,395 (GRCm38)	missense	probably benign	0.40
R4514:F5	UTSW	1	164,151,997 (GRCm38)	unclassified	probably benign
R4598:F5	UTSW	1	164,204,797 (GRCm38)	missense	probably benign	0.05
R4608:F5	UTSW	1	164,209,029 (GRCm38)	missense	probably benign	0.12
R4661:F5	UTSW	1	164,184,920 (GRCm38)	missense	probably damaging	1.00
R4689:F5	UTSW	1	164,151,973 (GRCm38)	unclassified	probably benign
R4716:F5	UTSW	1	164,193,919 (GRCm38)	missense	probably damaging	0.98
R4732:F5	UTSW	1	164,181,657 (GRCm38)	missense	probably damaging	1.00
R4733:F5	UTSW	1	164,181,657 (GRCm38)	missense	probably damaging	1.00
R4854:F5	UTSW	1	164,192,146 (GRCm38)	missense	probably damaging	1.00
R4908:F5	UTSW	1	164,211,820 (GRCm38)	missense	probably damaging	1.00
R4971:F5	UTSW	1	164,194,186 (GRCm38)	missense	probably benign
R5001:F5	UTSW	1	164,195,570 (GRCm38)	missense	probably benign	0.00
R5042:F5	UTSW	1	164,219,451 (GRCm38)	missense	probably damaging	1.00
R5056:F5	UTSW	1	164,192,032 (GRCm38)	missense	possibly damaging	0.60
R5061:F5	UTSW	1	164,194,180 (GRCm38)	missense	probably benign	0.00
R5143:F5	UTSW	1	164,211,828 (GRCm38)	missense	probably damaging	0.98
R5622:F5	UTSW	1	164,192,565 (GRCm38)	missense	probably benign	0.09
R5626:F5	UTSW	1	164,209,035 (GRCm38)	missense	probably damaging	0.98
R5658:F5	UTSW	1	164,192,338 (GRCm38)	missense	probably damaging	0.96
R5702:F5	UTSW	1	164,194,547 (GRCm38)	nonsense	probably null
R5795:F5	UTSW	1	164,152,009 (GRCm38)	missense	probably benign	0.09
R5884:F5	UTSW	1	164,195,646 (GRCm38)	missense	probably benign	0.01
R6036:F5	UTSW	1	164,184,996 (GRCm38)	missense	probably damaging	0.99
R6036:F5	UTSW	1	164,184,996 (GRCm38)	missense	probably damaging	0.99
R6151:F5	UTSW	1	164,190,187 (GRCm38)	missense	probably damaging	1.00
R6151:F5	UTSW	1	164,181,635 (GRCm38)	missense	probably damaging	1.00
R6345:F5	UTSW	1	164,191,951 (GRCm38)	missense	probably benign	0.13
R6391:F5	UTSW	1	164,193,493 (GRCm38)	missense	probably damaging	0.99
R6542:F5	UTSW	1	164,194,468 (GRCm38)	missense	probably benign	0.32
R6620:F5	UTSW	1	164,186,806 (GRCm38)	missense	probably damaging	1.00
R6750:F5	UTSW	1	164,193,507 (GRCm38)	missense	possibly damaging	0.58
R6754:F5	UTSW	1	164,193,763 (GRCm38)	missense	probably damaging	1.00
R6774:F5	UTSW	1	164,186,878 (GRCm38)	missense	probably damaging	1.00
R6802:F5	UTSW	1	164,179,356 (GRCm38)	missense	probably damaging	0.98
R6810:F5	UTSW	1	164,186,902 (GRCm38)	missense	probably damaging	1.00
R6983:F5	UTSW	1	164,194,129 (GRCm38)	missense	probably damaging	1.00
R7000:F5	UTSW	1	164,179,506 (GRCm38)	missense	probably damaging	1.00
R7151:F5	UTSW	1	164,201,661 (GRCm38)	missense	probably damaging	1.00
R7193:F5	UTSW	1	164,219,397 (GRCm38)	missense	probably damaging	1.00
R7230:F5	UTSW	1	164,184,953 (GRCm38)	missense	probably benign
R7324:F5	UTSW	1	164,193,581 (GRCm38)	small deletion	probably benign
R7350:F5	UTSW	1	164,192,708 (GRCm38)	missense	probably benign	0.08
R7466:F5	UTSW	1	164,193,328 (GRCm38)	missense	possibly damaging	0.61
R7503:F5	UTSW	1	164,192,210 (GRCm38)	missense	probably damaging	1.00
R7626:F5	UTSW	1	164,186,912 (GRCm38)	missense	possibly damaging	0.95
R7742:F5	UTSW	1	164,207,884 (GRCm38)	missense	possibly damaging	0.51
R7837:F5	UTSW	1	164,186,794 (GRCm38)	missense	probably damaging	1.00
R7848:F5	UTSW	1	164,161,877 (GRCm38)	missense	possibly damaging	0.94
R7925:F5	UTSW	1	164,176,366 (GRCm38)	critical splice donor site	probably null
R8053:F5	UTSW	1	164,192,769 (GRCm38)	missense	probably benign	0.26
R8094:F5	UTSW	1	164,208,940 (GRCm38)	missense	probably benign	0.06
R8175:F5	UTSW	1	164,192,265 (GRCm38)	nonsense	probably null
R8209:F5	UTSW	1	164,194,390 (GRCm38)	missense	probably benign	0.00
R8226:F5	UTSW	1	164,194,390 (GRCm38)	missense	probably benign	0.00
R8266:F5	UTSW	1	164,185,124 (GRCm38)	critical splice donor site	probably null
R8517:F5	UTSW	1	164,176,253 (GRCm38)	missense	probably damaging	0.99
R8684:F5	UTSW	1	164,217,542 (GRCm38)	missense	probably benign	0.01
R8941:F5	UTSW	1	164,198,871 (GRCm38)	missense	probably benign	0.19
R9130:F5	UTSW	1	164,174,261 (GRCm38)	missense	probably benign	0.37
R9181:F5	UTSW	1	164,192,326 (GRCm38)	missense	probably benign	0.00
R9186:F5	UTSW	1	164,193,901 (GRCm38)	missense	probably benign
R9233:F5	UTSW	1	164,219,451 (GRCm38)	missense	probably damaging	1.00
R9314:F5	UTSW	1	164,201,577 (GRCm38)	missense	probably benign	0.01
R9631:F5	UTSW	1	164,186,854 (GRCm38)	missense	probably damaging	1.00
R9655:F5	UTSW	1	164,194,161 (GRCm38)	missense	probably benign	0.15
X0024:F5	UTSW	1	164,192,988 (GRCm38)	missense	probably damaging	1.00
Z1088:F5	UTSW	1	164,154,385 (GRCm38)	missense	probably benign	0.04
Z1176:F5	UTSW	1	164,184,516 (GRCm38)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- GCAAGTGATCTGCAGAGAGC -3'
(R):5'- GAAGGTATCAAGGTTCTCCCAAGC -3'

Sequencing Primer
(F):5'- TGATCTGCAGAGAGCTAGATCTC -3'
(R):5'- AGCTTCAGCCCGATATAATCTC -3'

Posted On

2015-10-08