Incidental Mutation 'R4667:Wfdc3'
ID 352004
Institutional Source Beutler Lab
Gene Symbol Wfdc3
Ensembl Gene ENSMUSG00000076434
Gene Name WAP four-disulfide core domain 3
Synonyms 1700127F16Rik, 1700015L13Rik
MMRRC Submission 042012-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.096) question?
Stock # R4667 (G1)
Quality Score 173
Status Not validated
Chromosome 2
Chromosomal Location 164731180-164745931 bp(-) (GRCm38)
Type of Mutation start codon destroyed
DNA Base Change (assembly) T to C at 164743086 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Methionine to Valine at position 1 (M1V)
Ref Sequence ENSEMBL: ENSMUSP00000099385 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000017443] [ENSMUST00000103096] [ENSMUST00000109326] [ENSMUST00000109327] [ENSMUST00000109328]
AlphaFold Q14AE4
Predicted Effect probably benign
Transcript: ENSMUST00000017443
SMART Domains Protein: ENSMUSP00000017443
Gene: ENSMUSG00000017299

low complexity region 10 30 N/A INTRINSIC
Blast:UBCc 91 157 4e-16 BLAST
low complexity region 158 172 N/A INTRINSIC
Blast:UBCc 248 287 1e-14 BLAST
Predicted Effect probably null
Transcript: ENSMUST00000103096
AA Change: M1V

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
SMART Domains Protein: ENSMUSP00000099385
Gene: ENSMUSG00000076434
AA Change: M1V

signal peptide 1 16 N/A INTRINSIC
WAP 21 61 8.88e-4 SMART
WAP 65 106 4.29e-2 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000109326
SMART Domains Protein: ENSMUSP00000104949
Gene: ENSMUSG00000017299

low complexity region 10 30 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000109327
SMART Domains Protein: ENSMUSP00000104950
Gene: ENSMUSG00000017299

low complexity region 10 30 N/A INTRINSIC
Blast:UBCc 91 157 8e-17 BLAST
low complexity region 158 172 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000109328
Predicted Effect noncoding transcript
Transcript: ENSMUST00000130227
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 97.1%
  • 20x: 95.1%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 109 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930539E08Rik G T 17: 28,908,313 (GRCm38) Q241K possibly damaging Het
Acad8 A G 9: 26,990,627 (GRCm38) L147P probably damaging Het
Adgra3 T A 5: 49,978,956 (GRCm38) Y729F possibly damaging Het
Ago2 A G 15: 73,146,416 (GRCm38) Y58H probably damaging Het
Akap13 A G 7: 75,729,094 (GRCm38) T2128A probably damaging Het
Akap2 A T 4: 57,855,655 (GRCm38) D328V possibly damaging Het
Ankhd1 C A 18: 36,648,021 (GRCm38) P2042Q possibly damaging Het
Arhgef15 T C 11: 68,954,561 (GRCm38) K155R probably benign Het
Atp10b T C 11: 43,247,518 (GRCm38) F1209L probably damaging Het
B130006D01Rik A T 11: 95,726,509 (GRCm38) probably benign Het
Bmpr2 T C 1: 59,867,716 (GRCm38) L656S probably damaging Het
Btbd17 A G 11: 114,793,857 (GRCm38) F119L possibly damaging Het
Ccdc191 G T 16: 43,931,283 (GRCm38) K267N probably damaging Het
Ceacam20 T C 7: 19,986,027 (GRCm38) Y495H probably damaging Het
Celf2 T C 2: 6,721,528 (GRCm38) I47V probably benign Het
Chd9 T C 8: 91,033,800 (GRCm38) S2058P possibly damaging Het
Clcn6 T C 4: 148,024,167 (GRCm38) E135G possibly damaging Het
Cntn1 T A 15: 92,295,079 (GRCm38) N687K probably damaging Het
Col1a2 A T 6: 4,512,412 (GRCm38) M99L unknown Het
Cpeb2 T C 5: 43,233,892 (GRCm38) probably benign Het
Csn1s2b A G 5: 87,822,311 (GRCm38) T134A possibly damaging Het
Cst13 A T 2: 148,823,081 (GRCm38) probably benign Het
Cyp2c66 T A 19: 39,176,656 (GRCm38) D360E probably damaging Het
Dhx8 A G 11: 101,738,161 (GRCm38) S179G unknown Het
Dip2b A G 15: 100,151,360 (GRCm38) I212V probably benign Het
Dnah9 G A 11: 66,155,531 (GRCm38) H64Y probably benign Het
Dnal1 T C 12: 84,136,700 (GRCm38) probably benign Het
Dse T G 10: 34,153,012 (GRCm38) Y694S probably damaging Het
Dync2h1 T C 9: 7,051,411 (GRCm38) I3175V probably benign Het
Elf5 A G 2: 103,449,060 (GRCm38) N209D probably damaging Het
Elovl1 A G 4: 118,430,787 (GRCm38) Y40C probably damaging Het
Erp27 T C 6: 136,908,152 (GRCm38) E216G possibly damaging Het
F5 G A 1: 164,174,186 (GRCm38) V153I probably benign Het
Fam186a G A 15: 99,944,532 (GRCm38) T1277I possibly damaging Het
Fam90a1a A T 8: 21,963,346 (GRCm38) H239L possibly damaging Het
Fchsd2 G T 7: 101,250,449 (GRCm38) R334L probably damaging Het
Fermt3 T C 19: 7,002,920 (GRCm38) Y369C probably damaging Het
Fhod3 C T 18: 25,066,338 (GRCm38) P689S probably benign Het
Fnbp1l G T 3: 122,556,567 (GRCm38) Q332K probably benign Het
Frem3 T C 8: 80,663,420 (GRCm38) S1767P probably damaging Het
Ggt5 T C 10: 75,603,031 (GRCm38) L121P probably damaging Het
Gm609 A G 16: 45,444,163 (GRCm38) S11P probably benign Het
Gphn T C 12: 78,454,817 (GRCm38) S119P probably damaging Het
Herc2 A G 7: 56,131,253 (GRCm38) D1222G probably damaging Het
Hmx3 T C 7: 131,544,382 (GRCm38) I273T possibly damaging Het
Hnrnpu A G 1: 178,332,181 (GRCm38) probably benign Het
Hspg2 C T 4: 137,539,645 (GRCm38) T1987I possibly damaging Het
Ighv1-22 T A 12: 114,746,451 (GRCm38) Q58L probably damaging Het
Ighv14-3 T A 12: 114,060,255 (GRCm38) I7F probably benign Het
Kcns3 C A 12: 11,091,783 (GRCm38) R305L probably damaging Het
Kcnu1 C T 8: 25,910,921 (GRCm38) A699V possibly damaging Het
Kif22 A C 7: 127,033,328 (GRCm38) L270W probably damaging Het
Lrp2 G T 2: 69,489,298 (GRCm38) H1960Q probably benign Het
March7 C T 2: 60,241,050 (GRCm38) Q94* probably null Het
Mcoln3 A T 3: 146,131,204 (GRCm38) I264F probably benign Het
Mdn1 A C 4: 32,679,572 (GRCm38) T706P probably damaging Het
Mfsd2b A G 12: 4,867,636 (GRCm38) C137R probably benign Het
Mmp25 A G 17: 23,644,607 (GRCm38) V83A probably benign Het
Mocos T C 18: 24,666,434 (GRCm38) Y242H probably benign Het
Msh6 T C 17: 87,984,806 (GRCm38) S330P possibly damaging Het
Mtus2 T C 5: 148,298,260 (GRCm38) S1156P possibly damaging Het
Muc5b G A 7: 141,842,379 (GRCm38) R124H unknown Het
Mybbp1a G A 11: 72,447,971 (GRCm38) E775K possibly damaging Het
Myo10 A G 15: 25,793,153 (GRCm38) E1272G possibly damaging Het
Nars A G 18: 64,505,231 (GRCm38) S254P possibly damaging Het
Ncapd2 A G 6: 125,184,518 (GRCm38) I211T possibly damaging Het
Ncoa7 A T 10: 30,690,790 (GRCm38) W582R probably damaging Het
Npr3 T A 15: 11,905,467 (GRCm38) D58V possibly damaging Het
Nr3c1 G T 18: 39,428,727 (GRCm38) T430K probably benign Het
Odf2l A G 3: 145,128,040 (GRCm38) T111A probably benign Het
Ogdh G T 11: 6,340,600 (GRCm38) C406F probably benign Het
Olfml2a T G 2: 38,949,010 (GRCm38) S190A probably damaging Het
Olfr148 T C 9: 39,613,738 (GRCm38) M57T probably damaging Het
Olfr243 A G 7: 103,716,638 (GRCm38) T15A probably benign Het
Olfr870 T C 9: 20,171,098 (GRCm38) I158V probably benign Het
Olfr965 G T 9: 39,719,709 (GRCm38) V161F probably benign Het
Optn T C 2: 5,033,139 (GRCm38) K415E probably benign Het
Perm1 C A 4: 156,220,206 (GRCm38) S803* probably null Het
Pex14 T C 4: 148,984,085 (GRCm38) T84A probably benign Het
Pih1d2 T A 9: 50,620,952 (GRCm38) Y103* probably null Het
Pikfyve T A 1: 65,250,273 (GRCm38) C1235S probably damaging Het
Polr1a A G 6: 71,917,821 (GRCm38) N171S probably benign Het
Prrx1 A G 1: 163,254,047 (GRCm38) S201P probably benign Het
Psme2b A T 11: 48,945,666 (GRCm38) N151K probably benign Het
Serpinb5 A T 1: 106,872,295 (GRCm38) T72S probably benign Het
Sgsm1 A G 5: 113,260,047 (GRCm38) probably null Het
Sipa1l2 T C 8: 125,453,470 (GRCm38) R1063G possibly damaging Het
Slc19a3 T C 1: 83,022,799 (GRCm38) T166A probably benign Het
Slc5a4b T C 10: 76,075,045 (GRCm38) Y319C possibly damaging Het
Stard3nl T A 13: 19,376,519 (GRCm38) N29Y probably damaging Het
Sult6b2 G T 6: 142,801,695 (GRCm38) C109* probably null Het
Tcf25 A G 8: 123,397,025 (GRCm38) E467G possibly damaging Het
Tmem177 A T 1: 119,910,220 (GRCm38) V243D probably benign Het
Tmem2 G A 19: 21,797,351 (GRCm38) R119H probably benign Het
Tmem2 C T 19: 21,844,781 (GRCm38) A1180V probably benign Het
Top2b T G 14: 16,409,189 (GRCm38) I777M probably damaging Het
Tspan11 T A 6: 127,943,715 (GRCm38) C208* probably null Het
Ttc1 A G 11: 43,745,317 (GRCm38) V33A probably benign Het
Uck1 T A 2: 32,256,034 (GRCm38) H283L probably damaging Het
Utrn A C 10: 12,698,053 (GRCm38) V1091G probably benign Het
Vmn1r11 A T 6: 57,137,498 (GRCm38) H49L probably damaging Het
Vmn1r160 G T 7: 22,872,053 (GRCm38) S277I probably benign Het
Vmn1r18 A T 6: 57,390,084 (GRCm38) S162T probably benign Het
Vps37b A G 5: 124,010,732 (GRCm38) L80P probably damaging Het
Wrn A T 8: 33,324,338 (GRCm38) N116K probably benign Het
Wscd2 G T 5: 113,577,272 (GRCm38) G391V probably damaging Het
Zcchc11 G A 4: 108,495,159 (GRCm38) E357K probably damaging Het
Zfp286 A G 11: 62,780,602 (GRCm38) V215A probably benign Het
Zfp568 A G 7: 30,023,277 (GRCm38) H549R probably damaging Het
Other mutations in Wfdc3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00816:Wfdc3 APN 2 164,743,025 (GRCm38) intron probably benign
IGL01150:Wfdc3 APN 2 164,732,203 (GRCm38) splice site probably benign
R1572:Wfdc3 UTSW 2 164,744,194 (GRCm38) intron probably benign
R1689:Wfdc3 UTSW 2 164,734,191 (GRCm38) missense probably damaging 1.00
R6826:Wfdc3 UTSW 2 164,734,258 (GRCm38) missense possibly damaging 0.73
R6827:Wfdc3 UTSW 2 164,734,258 (GRCm38) missense possibly damaging 0.73
R6829:Wfdc3 UTSW 2 164,734,258 (GRCm38) missense possibly damaging 0.73
R6830:Wfdc3 UTSW 2 164,734,258 (GRCm38) missense possibly damaging 0.73
R9498:Wfdc3 UTSW 2 164,743,077 (GRCm38) missense possibly damaging 0.65
X0023:Wfdc3 UTSW 2 164,732,119 (GRCm38) missense probably benign 0.09
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2015-10-08