Mutagenetix > Incidental Mutation

Incidental Mutation 'R4656:Herc1'

352446

Institutional Source

Beutler Lab

Gene Symbol

Herc1

Ensembl Gene

ENSMUSG00000038664

Gene Name

HECT and RLD domain containing E3 ubiquitin protein ligase family member 1

Synonyms

tbl, D130015N03Rik, 2810449H11Rik

MMRRC Submission

041916-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4656 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

66350450-66508775 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 66394711 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 652 (T652A)

Ref Sequence

ENSEMBL: ENSMUSP00000044801 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042824]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000042824
AA Change: T652A

PolyPhen 2 Score 0.969 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000044801
Gene: ENSMUSG00000038664
AA Change: T652A

Domain	Start	End	E-Value	Type
low complexity region	79	90	N/A	INTRINSIC
low complexity region	136	147	N/A	INTRINSIC
Pfam:RCC1	476	526	5.4e-15	PFAM
Pfam:RCC1_2	513	542	1.3e-9	PFAM
Pfam:RCC1	529	576	5.5e-16	PFAM
Pfam:RCC1	579	629	1.5e-10	PFAM
Pfam:RCC1	632	680	3.6e-9	PFAM
Pfam:RCC1_2	667	696	2.2e-11	PFAM
Pfam:RCC1	683	733	1.2e-14	PFAM
low complexity region	787	807	N/A	INTRINSIC
low complexity region	852	864	N/A	INTRINSIC
low complexity region	1014	1025	N/A	INTRINSIC
low complexity region	1080	1100	N/A	INTRINSIC
low complexity region	1348	1378	N/A	INTRINSIC
low complexity region	1659	1676	N/A	INTRINSIC
low complexity region	1865	1874	N/A	INTRINSIC
low complexity region	2002	2030	N/A	INTRINSIC
SPRY	2067	2188	1.8e-30	SMART
coiled coil region	2251	2280	N/A	INTRINSIC
low complexity region	2410	2423	N/A	INTRINSIC
low complexity region	2613	2629	N/A	INTRINSIC
low complexity region	2633	2648	N/A	INTRINSIC
low complexity region	2650	2667	N/A	INTRINSIC
low complexity region	2736	2749	N/A	INTRINSIC
low complexity region	2882	2896	N/A	INTRINSIC
low complexity region	2924	2935	N/A	INTRINSIC
low complexity region	2971	2987	N/A	INTRINSIC
low complexity region	3045	3051	N/A	INTRINSIC
low complexity region	3168	3186	N/A	INTRINSIC
low complexity region	3191	3213	N/A	INTRINSIC
low complexity region	3364	3379	N/A	INTRINSIC
WD40	3415	3454	1.68e-6	SMART
WD40	3570	3608	3.68e1	SMART
WD40	3613	3652	4.3e-1	SMART
WD40	3657	3702	3.17e-2	SMART
WD40	3734	3773	8.29e-6	SMART
low complexity region	3950	3964	N/A	INTRINSIC
Pfam:RCC1_2	4079	4111	7.3e-9	PFAM
Pfam:RCC1	4098	4147	3.4e-16	PFAM
Pfam:RCC1_2	4134	4163	1.8e-7	PFAM
Pfam:RCC1	4150	4199	7.2e-16	PFAM
Pfam:RCC1	4204	4252	6.1e-12	PFAM
Pfam:RCC1	4255	4304	2.4e-7	PFAM
Pfam:RCC1_2	4291	4320	5.8e-12	PFAM
Pfam:RCC1	4307	4356	8.9e-16	PFAM
Blast:HECTc	4389	4423	2e-11	BLAST
HECTc	4497	4846	8.2e-148	SMART

Meta Mutation Damage Score

0.1347

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

96% (80/83)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gen encodes a member of the HERC protein family. This protein stimulates guanine nucleotide exchange on ARF1 and Rab proteins. This protein may be involved in membrane transport processes. [provided by RefSeq, Mar 2012]
PHENOTYPE: Homozygotes for this spontaneous mutation exhibit an abnormal cerebellar Purkinje cell layer and Purkinje cell degeneration. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810043G02Rik	G	A	10: 77,981,616 (GRCm38)	R59H	probably benign	Het
2310003L06Rik	G	A	5: 87,964,675 (GRCm38)		probably benign	Het
4931406B18Rik	T	A	7: 43,501,141 (GRCm38)	H69L	probably benign	Het
Ace2	T	C	X: 164,153,114 (GRCm38)	S84P	probably benign	Het
Adgb	A	C	10: 10,405,306 (GRCm38)	N656K	probably damaging	Het
Ago3	G	T	4: 126,363,752 (GRCm38)	Y495*	probably null	Het
Ahnak	G	A	19: 9,004,855 (GRCm38)	V1168M	possibly damaging	Het
Armc10	A	G	5: 21,661,550 (GRCm38)	R271G	probably benign	Het
Atp4a	T	G	7: 30,719,948 (GRCm38)		probably benign	Het
C87499	T	C	4: 88,629,965 (GRCm38)	T68A	probably benign	Het
Casp1	C	T	9: 5,304,324 (GRCm38)	P333S	probably damaging	Het
Ceacam9	C	T	7: 16,723,649 (GRCm38)	A34V	probably benign	Het
Ces1b	C	G	8: 93,057,414 (GRCm38)	E488Q	probably damaging	Het
Ces2h	A	T	8: 105,014,639 (GRCm38)	T88S	possibly damaging	Het
Col2a1	C	T	15: 97,976,176 (GRCm38)	G1375D	unknown	Het
Cyp1a1	T	C	9: 57,702,610 (GRCm38)	F436L	probably damaging	Het
Dcaf7	T	C	11: 106,053,798 (GRCm38)	V269A	probably damaging	Het
Disp2	T	C	2: 118,790,563 (GRCm38)	L592P	probably damaging	Het
Eda2r	T	A	X: 97,341,633 (GRCm38)	Q171L	probably damaging	Het
Egln2	A	G	7: 27,159,193 (GRCm38)	V408A	probably benign	Het
Gabpb2	A	G	3: 95,188,941 (GRCm38)	L325P	probably damaging	Het
Gigyf1	C	A	5: 137,525,215 (GRCm38)	Y936*	probably null	Het
Gm14149	A	T	2: 151,230,764 (GRCm38)		noncoding transcript	Het
Gnl2	C	T	4: 125,040,997 (GRCm38)	Q149*	probably null	Het
Gpr107	C	T	2: 31,214,249 (GRCm38)	T522M	probably damaging	Het
Grpr	T	A	X: 163,514,996 (GRCm38)	S351C	probably damaging	Het
Gsdma	T	C	11: 98,673,081 (GRCm38)	L287P	probably damaging	Het
Ifi204	C	A	1: 173,760,361 (GRCm38)		probably benign	Het
Irx2	T	C	13: 72,631,298 (GRCm38)	S234P	probably damaging	Het
Itgal	T	A	7: 127,322,553 (GRCm38)	D808E	probably damaging	Het
Krt13	C	A	11: 100,119,363 (GRCm38)	R264L	probably damaging	Het
March1	T	A	8: 66,386,419 (GRCm38)	L38I	probably benign	Het
Megf9	T	C	4: 70,448,767 (GRCm38)	H326R	probably damaging	Het
Mif4gd	G	T	11: 115,608,337 (GRCm38)		probably benign	Het
Mroh9	A	T	1: 163,066,024 (GRCm38)	M194K	probably damaging	Het
Nhlrc3	A	G	3: 53,463,080 (GRCm38)	S22P	probably damaging	Het
Nipal2	A	T	15: 34,577,568 (GRCm38)		probably null	Het
Nr1h4	A	G	10: 89,498,253 (GRCm38)	S78P	probably benign	Het
Ofcc1	G	A	13: 40,015,388 (GRCm38)	T841I	probably damaging	Het
Olfr390	A	T	11: 73,787,511 (GRCm38)	D191V	probably damaging	Het
Olfr607	C	T	7: 103,460,488 (GRCm38)	R235Q	probably benign	Het
Olfr908	A	T	9: 38,516,556 (GRCm38)	N175Y	probably damaging	Het
Pdzd2	A	G	15: 12,385,711 (GRCm38)	V991A	probably benign	Het
Pex1	G	A	5: 3,604,880 (GRCm38)		probably null	Het
Plch1	G	T	3: 63,704,177 (GRCm38)	A859E	probably damaging	Het
Ranbp2	A	G	10: 58,453,422 (GRCm38)	K84R	possibly damaging	Het
Rbm14	T	C	19: 4,811,435 (GRCm38)	Y25C	probably damaging	Het
Rmi2	A	G	16: 10,835,322 (GRCm38)	D78G	probably damaging	Het
Serpina3k	C	A	12: 104,345,273 (GRCm38)	T370K	probably damaging	Het
Shc2	A	T	10: 79,621,169 (GRCm38)	L538M	probably damaging	Het
Skint1	A	G	4: 112,021,477 (GRCm38)	K202R	probably damaging	Het
Slc22a29	C	A	19: 8,218,300 (GRCm38)	S125I	possibly damaging	Het
Slc5a9	C	A	4: 111,891,744 (GRCm38)		probably null	Het
Slco4c1	A	T	1: 96,841,245 (GRCm38)	D297E	probably benign	Het
Slco6d1	T	C	1: 98,423,203 (GRCm38)	F136S	probably benign	Het
Smg1	A	T	7: 118,212,951 (GRCm38)	V39E	probably benign	Het
Spns1	G	T	7: 126,374,302 (GRCm38)		probably benign	Het
Spsb1	A	G	4: 149,906,410 (GRCm38)		probably null	Het
Sspo	T	G	6: 48,454,076 (GRCm38)	I631S	possibly damaging	Het
Syne2	T	C	12: 76,031,373 (GRCm38)	L4694P	probably damaging	Het
Taf5l	T	C	8: 123,998,105 (GRCm38)	E325G	probably benign	Het
Tars	A	T	15: 11,394,264 (GRCm38)	S96T	probably damaging	Het
Tdrd12	T	C	7: 35,485,254 (GRCm38)	K745E	probably damaging	Het
Tenm3	A	G	8: 48,293,726 (GRCm38)	Y1015H	probably damaging	Het
Trpv3	T	A	11: 73,295,414 (GRCm38)	M677K	probably damaging	Het
Txlnb	A	T	10: 17,815,276 (GRCm38)	K191N	probably damaging	Het
Ubr1	A	G	2: 120,926,013 (GRCm38)	V711A	probably benign	Het
Uqcrb	T	C	13: 66,901,539 (GRCm38)	T41A	probably benign	Het
Usp6nl	A	G	2: 6,441,162 (GRCm38)	Y627C	probably damaging	Het
Vezf1	A	G	11: 88,074,667 (GRCm38)	D245G	probably damaging	Het
Vmn2r66	A	G	7: 85,011,996 (GRCm38)	W9R	possibly damaging	Het
Wdr17	C	T	8: 54,681,399 (GRCm38)	G349R	probably damaging	Het
Wdr35	T	A	12: 9,016,619 (GRCm38)	M749K	probably benign	Het
Wrn	A	T	8: 33,335,991 (GRCm38)		probably null	Het
Zfy1	T	G	Y: 729,626 (GRCm38)	T339P	unknown	Het

Other mutations in Herc1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00092:Herc1	APN	9	66,483,966 (GRCm38)	missense	probably benign	0.02
IGL00159:Herc1	APN	9	66,437,682 (GRCm38)	missense	possibly damaging	0.94
IGL00486:Herc1	APN	9	66,476,120 (GRCm38)	missense	probably benign
IGL00717:Herc1	APN	9	66,485,002 (GRCm38)	missense	probably damaging	1.00
IGL00766:Herc1	APN	9	66,450,741 (GRCm38)	missense	probably damaging	1.00
IGL00776:Herc1	APN	9	66,421,038 (GRCm38)	missense	probably benign
IGL00987:Herc1	APN	9	66,408,052 (GRCm38)	missense	probably benign	0.07
IGL01090:Herc1	APN	9	66,469,175 (GRCm38)	nonsense	probably null
IGL01098:Herc1	APN	9	66,461,922 (GRCm38)	critical splice donor site	probably null
IGL01106:Herc1	APN	9	66,476,438 (GRCm38)	splice site	probably benign
IGL01120:Herc1	APN	9	66,428,880 (GRCm38)	missense	probably benign
IGL01359:Herc1	APN	9	66,439,268 (GRCm38)	missense	probably benign	0.01
IGL01360:Herc1	APN	9	66,483,699 (GRCm38)	missense	probably benign
IGL01364:Herc1	APN	9	66,399,361 (GRCm38)	missense	probably benign	0.00
IGL01470:Herc1	APN	9	66,497,636 (GRCm38)	missense	possibly damaging	0.94
IGL01670:Herc1	APN	9	66,487,060 (GRCm38)	missense	probably damaging	1.00
IGL01825:Herc1	APN	9	66,399,807 (GRCm38)	missense	probably benign	0.00
IGL01903:Herc1	APN	9	66,386,872 (GRCm38)	nonsense	probably null
IGL01988:Herc1	APN	9	66,488,075 (GRCm38)	splice site	probably benign
IGL02074:Herc1	APN	9	66,450,983 (GRCm38)	missense	probably benign
IGL02089:Herc1	APN	9	66,480,869 (GRCm38)	missense	probably damaging	1.00
IGL02177:Herc1	APN	9	66,434,511 (GRCm38)	missense	probably benign
IGL02300:Herc1	APN	9	66,476,363 (GRCm38)	missense	probably benign	0.01
IGL02304:Herc1	APN	9	66,476,414 (GRCm38)	missense	probably benign	0.06
IGL02369:Herc1	APN	9	66,492,011 (GRCm38)	nonsense	probably null
IGL02445:Herc1	APN	9	66,433,482 (GRCm38)	missense	possibly damaging	0.95
IGL02447:Herc1	APN	9	66,497,328 (GRCm38)	missense	possibly damaging	0.59
IGL02549:Herc1	APN	9	66,399,901 (GRCm38)	missense	probably damaging	0.98
IGL02571:Herc1	APN	9	66,434,605 (GRCm38)	splice site	probably benign
IGL02709:Herc1	APN	9	66,497,680 (GRCm38)	missense	probably damaging	0.97
IGL02717:Herc1	APN	9	66,371,921 (GRCm38)	nonsense	probably null
IGL02726:Herc1	APN	9	66,441,988 (GRCm38)	missense	probably benign	0.37
IGL02733:Herc1	APN	9	66,450,992 (GRCm38)	missense	probably benign
IGL02963:Herc1	APN	9	66,388,823 (GRCm38)	missense	probably damaging	0.99
IGL03101:Herc1	APN	9	66,487,997 (GRCm38)	missense	probably benign
IGL03193:Herc1	APN	9	66,402,680 (GRCm38)	missense	probably benign
IGL03203:Herc1	APN	9	66,388,900 (GRCm38)	critical splice donor site	probably null
IGL03216:Herc1	APN	9	66,478,946 (GRCm38)	missense	probably benign	0.06
IGL03282:Herc1	APN	9	66,451,459 (GRCm38)	missense	probably benign	0.05
IGL03295:Herc1	APN	9	66,396,703 (GRCm38)	missense	possibly damaging	0.56
cradle	UTSW	9	66,483,866 (GRCm38)	splice site	probably null
miracles	UTSW	9	66,462,837 (GRCm38)	nonsense	probably null
newton	UTSW	9	66,467,803 (GRCm38)	missense	probably damaging	1.00
R0907_Herc1_362	UTSW	9	66,433,428 (GRCm38)	missense	possibly damaging	0.94
R4427_Herc1_231	UTSW	9	66,496,005 (GRCm38)	missense	probably damaging	1.00
R5026_Herc1_363	UTSW	9	66,486,126 (GRCm38)	missense	probably benign	0.03
stables	UTSW	9	66,479,453 (GRCm38)	missense	probably benign	0.13
strangle	UTSW	9	66,501,188 (GRCm38)	frame shift	probably null
IGL03134:Herc1	UTSW	9	66,434,063 (GRCm38)	critical splice acceptor site	probably benign
PIT4243001:Herc1	UTSW	9	66,372,207 (GRCm38)	missense	probably benign	0.00
PIT4486001:Herc1	UTSW	9	66,372,389 (GRCm38)	missense	probably damaging	1.00
PIT4696001:Herc1	UTSW	9	66,479,009 (GRCm38)	missense	probably damaging	1.00
R0044:Herc1	UTSW	9	66,448,175 (GRCm38)	missense	probably benign	0.04
R0044:Herc1	UTSW	9	66,448,175 (GRCm38)	missense	probably benign	0.04
R0052:Herc1	UTSW	9	66,400,156 (GRCm38)	missense	probably damaging	0.99
R0114:Herc1	UTSW	9	66,461,846 (GRCm38)	missense	probably damaging	0.99
R0129:Herc1	UTSW	9	66,448,075 (GRCm38)	missense	probably damaging	1.00
R0131:Herc1	UTSW	9	66,480,910 (GRCm38)	missense	probably benign	0.00
R0131:Herc1	UTSW	9	66,480,910 (GRCm38)	missense	probably benign	0.00
R0132:Herc1	UTSW	9	66,480,910 (GRCm38)	missense	probably benign	0.00
R0158:Herc1	UTSW	9	66,495,921 (GRCm38)	nonsense	probably null
R0333:Herc1	UTSW	9	66,464,699 (GRCm38)	splice site	probably null
R0384:Herc1	UTSW	9	66,481,050 (GRCm38)	splice site	probably benign
R0419:Herc1	UTSW	9	66,446,074 (GRCm38)	splice site	probably benign
R0453:Herc1	UTSW	9	66,399,772 (GRCm38)	missense	probably benign	0.20
R0458:Herc1	UTSW	9	66,476,381 (GRCm38)	missense	probably benign	0.12
R0490:Herc1	UTSW	9	66,484,999 (GRCm38)	missense	probably damaging	1.00
R0506:Herc1	UTSW	9	66,448,159 (GRCm38)	missense	probably damaging	0.99
R0513:Herc1	UTSW	9	66,445,645 (GRCm38)	missense	possibly damaging	0.96
R0628:Herc1	UTSW	9	66,450,881 (GRCm38)	missense	probably benign	0.35
R0666:Herc1	UTSW	9	66,484,888 (GRCm38)	splice site	probably benign
R0674:Herc1	UTSW	9	66,501,192 (GRCm38)	missense	probably damaging	0.99
R0682:Herc1	UTSW	9	66,481,981 (GRCm38)	missense	possibly damaging	0.95
R0690:Herc1	UTSW	9	66,386,838 (GRCm38)	nonsense	probably null
R0701:Herc1	UTSW	9	66,487,950 (GRCm38)	missense	probably damaging	1.00
R0766:Herc1	UTSW	9	66,504,840 (GRCm38)	missense	probably damaging	1.00
R0850:Herc1	UTSW	9	66,466,670 (GRCm38)	missense	probably damaging	1.00
R0907:Herc1	UTSW	9	66,433,428 (GRCm38)	missense	possibly damaging	0.94
R0972:Herc1	UTSW	9	66,372,145 (GRCm38)	missense	probably damaging	1.00
R0976:Herc1	UTSW	9	66,439,878 (GRCm38)	missense	possibly damaging	0.74
R1027:Herc1	UTSW	9	66,455,968 (GRCm38)	missense	probably benign
R1200:Herc1	UTSW	9	66,486,124 (GRCm38)	missense	probably damaging	1.00
R1226:Herc1	UTSW	9	66,416,263 (GRCm38)	missense	probably benign	0.00
R1364:Herc1	UTSW	9	66,400,093 (GRCm38)	missense	probably damaging	1.00
R1395:Herc1	UTSW	9	66,439,181 (GRCm38)	missense	probably benign	0.13
R1432:Herc1	UTSW	9	66,465,469 (GRCm38)	missense	probably benign	0.13
R1440:Herc1	UTSW	9	66,467,803 (GRCm38)	missense	probably damaging	1.00
R1476:Herc1	UTSW	9	66,508,266 (GRCm38)	missense	probably damaging	1.00
R1590:Herc1	UTSW	9	66,491,953 (GRCm38)	splice site	probably benign
R1634:Herc1	UTSW	9	66,473,538 (GRCm38)	missense	possibly damaging	0.51
R1700:Herc1	UTSW	9	66,450,678 (GRCm38)	splice site	probably null
R1753:Herc1	UTSW	9	66,502,084 (GRCm38)	critical splice donor site	probably null
R1753:Herc1	UTSW	9	66,469,010 (GRCm38)	missense	probably damaging	1.00
R1796:Herc1	UTSW	9	66,388,856 (GRCm38)	nonsense	probably null
R1830:Herc1	UTSW	9	66,497,599 (GRCm38)	missense	possibly damaging	0.95
R1855:Herc1	UTSW	9	66,391,426 (GRCm38)	missense	possibly damaging	0.95
R1866:Herc1	UTSW	9	66,450,791 (GRCm38)	missense	probably damaging	1.00
R1894:Herc1	UTSW	9	66,479,461 (GRCm38)	missense	probably damaging	1.00
R1918:Herc1	UTSW	9	66,476,126 (GRCm38)	splice site	probably null
R1999:Herc1	UTSW	9	66,486,078 (GRCm38)	missense	probably benign	0.07
R2034:Herc1	UTSW	9	66,441,972 (GRCm38)	missense	probably benign	0.01
R2138:Herc1	UTSW	9	66,470,307 (GRCm38)	missense	possibly damaging	0.94
R2186:Herc1	UTSW	9	66,439,901 (GRCm38)	missense	probably benign	0.45
R2192:Herc1	UTSW	9	66,465,406 (GRCm38)	missense	probably damaging	0.99
R2312:Herc1	UTSW	9	66,508,281 (GRCm38)	nonsense	probably null
R2338:Herc1	UTSW	9	66,428,969 (GRCm38)	missense	possibly damaging	0.69
R3035:Herc1	UTSW	9	66,483,935 (GRCm38)	missense	possibly damaging	0.89
R3732:Herc1	UTSW	9	66,445,640 (GRCm38)	missense	probably damaging	1.00
R3732:Herc1	UTSW	9	66,445,640 (GRCm38)	missense	probably damaging	1.00
R3733:Herc1	UTSW	9	66,445,640 (GRCm38)	missense	probably damaging	1.00
R3917:Herc1	UTSW	9	66,434,466 (GRCm38)	missense	possibly damaging	0.94
R3953:Herc1	UTSW	9	66,433,793 (GRCm38)	nonsense	probably null
R4073:Herc1	UTSW	9	66,418,492 (GRCm38)	missense	probably benign	0.12
R4075:Herc1	UTSW	9	66,418,492 (GRCm38)	missense	probably benign	0.12
R4241:Herc1	UTSW	9	66,448,348 (GRCm38)	frame shift	probably null
R4260:Herc1	UTSW	9	66,448,348 (GRCm38)	frame shift	probably null
R4261:Herc1	UTSW	9	66,448,348 (GRCm38)	frame shift	probably null
R4300:Herc1	UTSW	9	66,489,406 (GRCm38)	missense	probably damaging	1.00
R4398:Herc1	UTSW	9	66,479,453 (GRCm38)	missense	probably benign	0.13
R4426:Herc1	UTSW	9	66,496,005 (GRCm38)	missense	probably damaging	1.00
R4427:Herc1	UTSW	9	66,496,005 (GRCm38)	missense	probably damaging	1.00
R4590:Herc1	UTSW	9	66,437,664 (GRCm38)	missense	probably damaging	0.97
R4630:Herc1	UTSW	9	66,433,714 (GRCm38)	splice site	probably null
R4658:Herc1	UTSW	9	66,479,491 (GRCm38)	missense	possibly damaging	0.50
R4663:Herc1	UTSW	9	66,433,378 (GRCm38)	missense	probably damaging	0.98
R4675:Herc1	UTSW	9	66,391,458 (GRCm38)	missense	probably damaging	1.00
R4678:Herc1	UTSW	9	66,416,269 (GRCm38)	missense	probably benign	0.00
R4754:Herc1	UTSW	9	66,501,206 (GRCm38)	missense	probably benign	0.00
R4766:Herc1	UTSW	9	66,441,929 (GRCm38)	missense	probably benign	0.00
R4792:Herc1	UTSW	9	66,495,984 (GRCm38)	missense	possibly damaging	0.67
R4828:Herc1	UTSW	9	66,497,343 (GRCm38)	splice site	probably null
R4832:Herc1	UTSW	9	66,495,971 (GRCm38)	missense	probably benign	0.11
R4879:Herc1	UTSW	9	66,462,837 (GRCm38)	nonsense	probably null
R4948:Herc1	UTSW	9	66,484,902 (GRCm38)	missense	probably benign
R5021:Herc1	UTSW	9	66,470,326 (GRCm38)	missense	possibly damaging	0.48
R5022:Herc1	UTSW	9	66,470,326 (GRCm38)	missense	possibly damaging	0.48
R5023:Herc1	UTSW	9	66,470,326 (GRCm38)	missense	possibly damaging	0.48
R5024:Herc1	UTSW	9	66,470,326 (GRCm38)	missense	possibly damaging	0.48
R5025:Herc1	UTSW	9	66,470,326 (GRCm38)	missense	possibly damaging	0.48
R5026:Herc1	UTSW	9	66,486,126 (GRCm38)	missense	probably benign	0.03
R5027:Herc1	UTSW	9	66,473,529 (GRCm38)	missense	probably benign	0.01
R5027:Herc1	UTSW	9	66,504,618 (GRCm38)	missense	probably damaging	0.98
R5038:Herc1	UTSW	9	66,476,460 (GRCm38)	intron	probably benign
R5041:Herc1	UTSW	9	66,429,045 (GRCm38)	missense	possibly damaging	0.86
R5053:Herc1	UTSW	9	66,470,326 (GRCm38)	missense	possibly damaging	0.48
R5137:Herc1	UTSW	9	66,448,223 (GRCm38)	missense	probably benign
R5197:Herc1	UTSW	9	66,448,504 (GRCm38)	missense	probably damaging	0.99
R5207:Herc1	UTSW	9	66,399,869 (GRCm38)	nonsense	probably null
R5247:Herc1	UTSW	9	66,434,551 (GRCm38)	missense	probably benign	0.01
R5267:Herc1	UTSW	9	66,461,809 (GRCm38)	missense	probably damaging	1.00
R5274:Herc1	UTSW	9	66,399,409 (GRCm38)	missense	probably benign
R5375:Herc1	UTSW	9	66,467,887 (GRCm38)	missense	probably damaging	0.99
R5401:Herc1	UTSW	9	66,502,056 (GRCm38)	missense	probably damaging	1.00
R5560:Herc1	UTSW	9	66,451,119 (GRCm38)	missense	probably benign	0.02
R5566:Herc1	UTSW	9	66,465,537 (GRCm38)	missense	possibly damaging	0.95
R5577:Herc1	UTSW	9	66,481,981 (GRCm38)	missense	probably damaging	0.99
R5596:Herc1	UTSW	9	66,434,063 (GRCm38)	critical splice acceptor site	probably benign
R5665:Herc1	UTSW	9	66,465,435 (GRCm38)	missense	probably damaging	1.00
R5744:Herc1	UTSW	9	66,508,193 (GRCm38)	missense	probably damaging	1.00
R5802:Herc1	UTSW	9	66,462,878 (GRCm38)	missense	probably damaging	1.00
R5822:Herc1	UTSW	9	66,445,612 (GRCm38)	missense	probably benign	0.00
R5954:Herc1	UTSW	9	66,451,492 (GRCm38)	splice site	probably benign
R5977:Herc1	UTSW	9	66,433,322 (GRCm38)	missense	possibly damaging	0.77
R6022:Herc1	UTSW	9	66,483,685 (GRCm38)	missense	probably damaging	1.00
R6043:Herc1	UTSW	9	66,408,154 (GRCm38)	missense	probably benign
R6046:Herc1	UTSW	9	66,445,549 (GRCm38)	missense	probably damaging	0.99
R6089:Herc1	UTSW	9	66,445,532 (GRCm38)	missense	probably damaging	1.00
R6123:Herc1	UTSW	9	66,497,250 (GRCm38)	missense	probably damaging	0.97
R6155:Herc1	UTSW	9	66,433,423 (GRCm38)	missense	possibly damaging	0.95
R6190:Herc1	UTSW	9	66,376,381 (GRCm38)	missense	possibly damaging	0.56
R6220:Herc1	UTSW	9	66,433,788 (GRCm38)	missense	probably damaging	1.00
R6265:Herc1	UTSW	9	66,372,016 (GRCm38)	missense	probably benign	0.05
R6348:Herc1	UTSW	9	66,487,976 (GRCm38)	missense	possibly damaging	0.77
R6362:Herc1	UTSW	9	66,471,908 (GRCm38)	missense	probably damaging	1.00
R6394:Herc1	UTSW	9	66,395,059 (GRCm38)	missense	probably damaging	0.99
R6434:Herc1	UTSW	9	66,486,182 (GRCm38)	missense	probably damaging	0.99
R6483:Herc1	UTSW	9	66,448,529 (GRCm38)	missense	possibly damaging	0.64
R6607:Herc1	UTSW	9	66,418,567 (GRCm38)	missense	probably benign	0.02
R6633:Herc1	UTSW	9	66,439,252 (GRCm38)	nonsense	probably null
R6634:Herc1	UTSW	9	66,437,744 (GRCm38)	missense	probably benign
R6693:Herc1	UTSW	9	66,478,976 (GRCm38)	missense	probably damaging	0.99
R6695:Herc1	UTSW	9	66,483,866 (GRCm38)	splice site	probably null
R6748:Herc1	UTSW	9	66,501,188 (GRCm38)	frame shift	probably null
R6750:Herc1	UTSW	9	66,501,188 (GRCm38)	frame shift	probably null
R6751:Herc1	UTSW	9	66,501,188 (GRCm38)	frame shift	probably null
R6774:Herc1	UTSW	9	66,501,188 (GRCm38)	frame shift	probably null
R6785:Herc1	UTSW	9	66,501,188 (GRCm38)	frame shift	probably null
R6786:Herc1	UTSW	9	66,501,188 (GRCm38)	frame shift	probably null
R6856:Herc1	UTSW	9	66,397,898 (GRCm38)	missense	probably benign	0.05
R6966:Herc1	UTSW	9	66,411,065 (GRCm38)	missense	probably benign	0.07
R7020:Herc1	UTSW	9	66,486,078 (GRCm38)	missense	probably benign	0.07
R7109:Herc1	UTSW	9	66,481,889 (GRCm38)	missense	probably benign	0.03
R7122:Herc1	UTSW	9	66,399,774 (GRCm38)	missense	possibly damaging	0.69
R7209:Herc1	UTSW	9	66,385,032 (GRCm38)	missense	possibly damaging	0.95
R7222:Herc1	UTSW	9	66,467,499 (GRCm38)	missense	probably damaging	0.98
R7303:Herc1	UTSW	9	66,450,816 (GRCm38)	missense	possibly damaging	0.93
R7305:Herc1	UTSW	9	66,461,868 (GRCm38)	missense
R7438:Herc1	UTSW	9	66,394,756 (GRCm38)	missense	probably benign	0.00
R7535:Herc1	UTSW	9	66,474,853 (GRCm38)	missense	probably damaging	1.00
R7585:Herc1	UTSW	9	66,445,547 (GRCm38)	missense	probably damaging	1.00
R7603:Herc1	UTSW	9	66,451,383 (GRCm38)	nonsense	probably null
R7670:Herc1	UTSW	9	66,416,347 (GRCm38)	missense	probably damaging	0.99
R7705:Herc1	UTSW	9	66,439,834 (GRCm38)	missense	possibly damaging	0.86
R7723:Herc1	UTSW	9	66,371,876 (GRCm38)	missense	probably benign	0.24
R7730:Herc1	UTSW	9	66,493,190 (GRCm38)	small deletion	probably benign
R7880:Herc1	UTSW	9	66,508,224 (GRCm38)	missense	probably damaging	0.99
R7958:Herc1	UTSW	9	66,486,193 (GRCm38)	missense	probably damaging	1.00
R7976:Herc1	UTSW	9	66,434,270 (GRCm38)	missense	possibly damaging	0.94
R8006:Herc1	UTSW	9	66,445,560 (GRCm38)	nonsense	probably null
R8084:Herc1	UTSW	9	66,475,935 (GRCm38)	missense	probably benign	0.45
R8094:Herc1	UTSW	9	66,493,180 (GRCm38)	missense	probably damaging	0.98
R8099:Herc1	UTSW	9	66,372,140 (GRCm38)	missense	probably damaging	1.00
R8151:Herc1	UTSW	9	66,433,791 (GRCm38)	missense	probably damaging	0.98
R8159:Herc1	UTSW	9	66,461,721 (GRCm38)	missense	probably null
R8190:Herc1	UTSW	9	66,418,451 (GRCm38)	missense	probably benign	0.00
R8213:Herc1	UTSW	9	66,450,888 (GRCm38)	missense	probably damaging	0.99
R8230:Herc1	UTSW	9	66,470,316 (GRCm38)	missense	probably damaging	0.99
R8265:Herc1	UTSW	9	66,386,704 (GRCm38)	nonsense	probably null
R8270:Herc1	UTSW	9	66,487,950 (GRCm38)	missense	probably damaging	1.00
R8353:Herc1	UTSW	9	66,508,289 (GRCm38)	missense	possibly damaging	0.88
R8423:Herc1	UTSW	9	66,508,160 (GRCm38)	missense	probably damaging	0.99
R8506:Herc1	UTSW	9	66,473,581 (GRCm38)	missense	possibly damaging	0.52
R8523:Herc1	UTSW	9	66,450,942 (GRCm38)	missense	probably benign
R8530:Herc1	UTSW	9	66,418,628 (GRCm38)	missense	probably benign
R8545:Herc1	UTSW	9	66,371,975 (GRCm38)	nonsense	probably null
R8682:Herc1	UTSW	9	66,462,848 (GRCm38)	missense
R8720:Herc1	UTSW	9	66,481,823 (GRCm38)	missense	probably benign	0.38
R8792:Herc1	UTSW	9	66,465,486 (GRCm38)	missense	probably damaging	1.00
R8915:Herc1	UTSW	9	66,411,174 (GRCm38)	missense	probably damaging	1.00
R8964:Herc1	UTSW	9	66,445,590 (GRCm38)	missense	probably damaging	1.00
R9056:Herc1	UTSW	9	66,473,500 (GRCm38)	missense	probably benign	0.10
R9158:Herc1	UTSW	9	66,469,118 (GRCm38)	missense	probably benign	0.00
R9167:Herc1	UTSW	9	66,504,618 (GRCm38)	missense	possibly damaging	0.75
R9192:Herc1	UTSW	9	66,414,131 (GRCm38)	missense	probably benign	0.35
R9252:Herc1	UTSW	9	66,402,552 (GRCm38)	missense	probably damaging	1.00
R9260:Herc1	UTSW	9	66,418,409 (GRCm38)	nonsense	probably null
R9261:Herc1	UTSW	9	66,504,847 (GRCm38)	missense	probably damaging	0.98
R9430:Herc1	UTSW	9	66,418,503 (GRCm38)	nonsense	probably null
R9519:Herc1	UTSW	9	66,400,074 (GRCm38)	missense	probably damaging	0.97
R9563:Herc1	UTSW	9	66,386,911 (GRCm38)	critical splice donor site	probably null
R9589:Herc1	UTSW	9	66,465,558 (GRCm38)	missense	possibly damaging	0.95
R9600:Herc1	UTSW	9	66,397,312 (GRCm38)	missense	possibly damaging	0.95
R9659:Herc1	UTSW	9	66,399,903 (GRCm38)	missense	probably benign	0.03
R9740:Herc1	UTSW	9	66,448,514 (GRCm38)	missense	probably damaging	1.00
R9774:Herc1	UTSW	9	66,464,750 (GRCm38)	missense	probably null
R9781:Herc1	UTSW	9	66,372,722 (GRCm38)	missense	probably benign
R9788:Herc1	UTSW	9	66,399,903 (GRCm38)	missense	probably benign	0.03
RF023:Herc1	UTSW	9	66,458,334 (GRCm38)	missense
X0011:Herc1	UTSW	9	66,400,159 (GRCm38)	missense	probably benign	0.28
X0067:Herc1	UTSW	9	66,448,524 (GRCm38)	missense	probably benign	0.03
Z1176:Herc1	UTSW	9	66,434,576 (GRCm38)	missense	probably benign
Z1177:Herc1	UTSW	9	66,471,911 (GRCm38)	missense	probably damaging	0.99
Z1177:Herc1	UTSW	9	66,458,425 (GRCm38)	missense	probably null

Predicted Primers

PCR Primer
(F):5'- AGTCTTCTGAGAGTACAATGACCATC -3'
(R):5'- CATGGAGTTATTGCCCCAGG -3'

Sequencing Primer
(F):5'- GGAAGGTACCACAACTTGAT -3'
(R):5'- CCCCAGGCATAAACTTCATTATC -3'

Posted On

2015-10-08