Mutagenetix > Incidental Mutation

Incidental Mutation 'R4658:Fryl'

352586

Institutional Source

Beutler Lab

Gene Symbol

Fryl

Ensembl Gene

ENSMUSG00000070733

Gene Name

FRY like transcription coactivator

Synonyms

9030227G01Rik, 2310004H21Rik, 2510002A14Rik

MMRRC Submission

041918-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.787) question?

Stock #

R4658 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

73177534-73414042 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 73238396 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 1450 (T1450K)

Ref Sequence

ENSEMBL: ENSMUSP00000098687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094700] [ENSMUST00000101127]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000094700
AA Change: T1450K

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000092289
Gene: ENSMUSG00000070733
AA Change: T1450K

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	117	649	5.7e-176	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	896	1141	1.3e-5	PFAM
Pfam:MOR2-PAG1_mid	1145	1331	2e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1450	1.2e-5	PFAM
low complexity region	1476	1487	N/A	INTRINSIC
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1590	1660	1.1e-5	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	3.2e-15	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2002	2255	9.9e-78	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000101127
AA Change: T1450K

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000098687
Gene: ENSMUSG00000070733
AA Change: T1450K

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	116	649	3e-172	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	898	1115	7.8e-6	PFAM
Pfam:MOR2-PAG1_mid	1147	1331	9.5e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1503	1.1e-5	PFAM
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1589	1664	6e-6	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	1.6e-14	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	1998	2260	4.4e-76	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000175890

Predicted Effect

unknown
Transcript: ENSMUST00000202381
AA Change: T319K

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.5%

Validation Efficiency

99% (88/89)

MGI Phenotype

PHENOTYPE: Most mice homozygous for a knock-out allele exhibit postnatal lethality and defects in kidney development; rare survivors display growth retardation, decreased body weight, and premature death associated with chronic hydronephrosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	A	T	5: 114,338,625 (GRCm39)	R778S	probably damaging	Het
Adam17	A	G	12: 21,382,161 (GRCm39)	C567R	probably damaging	Het
Ankrd28	G	A	14: 31,432,825 (GRCm39)	A758V	probably damaging	Het
Atrn	T	C	2: 130,775,349 (GRCm39)	Y151H	probably damaging	Het
B3gat3	A	G	19: 8,902,996 (GRCm39)	T118A	possibly damaging	Het
Camta1	A	G	4: 151,228,367 (GRCm39)	C822R	probably damaging	Het
Capn15	G	T	17: 26,179,742 (GRCm39)	Q807K	probably benign	Het
Clec12a	T	C	6: 129,331,493 (GRCm39)	Y145H	probably damaging	Het
Clk1	T	C	1: 58,452,146 (GRCm39)	I393V	probably benign	Het
Cpm	A	G	10: 117,503,956 (GRCm39)	I121V	probably benign	Het
Cux1	A	G	5: 136,279,448 (GRCm39)	I405T	possibly damaging	Het
Dnah3	A	G	7: 119,549,874 (GRCm39)	S3471P	probably damaging	Het
Dok6	A	G	18: 89,491,971 (GRCm39)		probably benign	Het
Eif4g1	A	T	16: 20,504,684 (GRCm39)	D1124V	possibly damaging	Het
Eif4g3	A	G	4: 137,933,443 (GRCm39)	E1756G	probably damaging	Het
Exo5	A	G	4: 120,779,748 (GRCm39)	V39A	probably benign	Het
Fmnl1	A	T	11: 103,088,520 (GRCm39)	I90F	probably damaging	Het
Gde1	T	C	7: 118,293,751 (GRCm39)	M91V	probably benign	Het
Gimd1	T	C	3: 132,350,343 (GRCm39)	I84T	probably damaging	Het
Gm13889	C	T	2: 93,787,453 (GRCm39)		probably benign	Het
Gm6445	T	A	19: 9,585,561 (GRCm39)		noncoding transcript	Het
Gm8113	T	C	14: 44,169,867 (GRCm39)	S483P	probably damaging	Het
Grik2	T	C	10: 49,399,888 (GRCm39)	I281V	possibly damaging	Het
Grik5	T	C	7: 24,760,152 (GRCm39)		probably benign	Het
Herc1	A	T	9: 66,386,773 (GRCm39)	I3796F	possibly damaging	Het
Hoxb13	A	T	11: 96,085,309 (GRCm39)	D14V	probably benign	Het
Hspg2	A	G	4: 137,261,041 (GRCm39)	Y1645C	probably damaging	Het
Igkv8-16	G	T	6: 70,363,762 (GRCm39)	R87S	probably damaging	Het
Ints1	A	T	5: 139,760,054 (GRCm39)	V140E	possibly damaging	Het
Kbtbd11	C	A	8: 15,078,917 (GRCm39)	D505E	possibly damaging	Het
Kcnu1	G	A	8: 26,427,583 (GRCm39)	C300Y	probably damaging	Het
Kmt2d	G	C	15: 98,750,410 (GRCm39)		probably benign	Het
Lats1	T	C	10: 7,578,493 (GRCm39)	V539A	probably benign	Het
Lipo5	C	T	19: 33,441,922 (GRCm39)	G200D	unknown	Het
Lmo7	C	A	14: 102,124,393 (GRCm39)	A284D	probably damaging	Het
Lyst	G	A	13: 13,809,968 (GRCm39)	R546H	probably damaging	Het
Mcpt8	T	C	14: 56,321,285 (GRCm39)	M60V	possibly damaging	Het
Mdn1	A	G	4: 32,730,749 (GRCm39)		probably null	Het
Mphosph10	A	G	7: 64,038,722 (GRCm39)		probably null	Het
Muc5b	G	A	7: 141,395,135 (GRCm39)	S47N	unknown	Het
Notch3	A	T	17: 32,373,737 (GRCm39)	N490K	probably damaging	Het
Nr1d1	G	A	11: 98,662,738 (GRCm39)	S85L	possibly damaging	Het
Obscn	T	A	11: 58,945,114 (GRCm39)	R4635*	probably null	Het
Or10al5	G	T	17: 38,063,054 (GRCm39)	C103F	probably damaging	Het
Or13f5	T	C	4: 52,826,240 (GRCm39)	L281P	probably damaging	Het
Or9i16	T	C	19: 13,864,912 (GRCm39)	I221V	probably benign	Het
Pappa	G	A	4: 65,233,033 (GRCm39)		probably null	Het
Pcdhb17	G	A	18: 37,619,652 (GRCm39)	G481S	probably damaging	Het
Pde1a	TCC	TC	2: 79,728,525 (GRCm39)		probably benign	Het
Phf3	A	T	1: 30,902,169 (GRCm39)	M48K	probably damaging	Het
Pira2	A	T	7: 3,843,933 (GRCm39)	V613E	probably damaging	Het
Poc1a	T	C	9: 106,226,887 (GRCm39)	S327P	possibly damaging	Het
Ptpn9	A	G	9: 56,927,321 (GRCm39)	H66R	probably benign	Het
Rabgap1	C	T	2: 37,377,561 (GRCm39)	R353*	probably null	Het
Rcc1l	G	T	5: 134,200,729 (GRCm39)	N134K	probably damaging	Het
Rims1	G	A	1: 22,497,793 (GRCm39)	T787I	probably damaging	Het
Rreb1	T	G	13: 38,132,777 (GRCm39)	S1651A	probably damaging	Het
Rsl1d1	T	C	16: 11,019,238 (GRCm39)	D100G	probably damaging	Het
Samd4	C	T	14: 47,301,703 (GRCm39)	R147C	probably damaging	Het
Serpinb6e	T	C	13: 34,025,299 (GRCm39)		probably benign	Het
Ska1	T	C	18: 74,330,111 (GRCm39)	I210V	probably benign	Het
Slc17a1	A	G	13: 24,062,543 (GRCm39)	I237V	probably benign	Het
Slc22a4	A	T	11: 53,888,336 (GRCm39)	S231T	probably benign	Het
Slc7a4	T	A	16: 17,393,797 (GRCm39)	M66L	probably damaging	Het
Snapc1	A	G	12: 74,030,642 (GRCm39)	T381A	possibly damaging	Het
St6galnac2	C	T	11: 116,575,351 (GRCm39)		probably benign	Het
Taar2	C	T	10: 23,817,401 (GRCm39)	L314F	probably benign	Het
Tmem74b	A	G	2: 151,548,561 (GRCm39)	D96G	probably damaging	Het
Tnfrsf17	T	C	16: 11,131,833 (GRCm39)	F6S	probably benign	Het
Tpp2	G	T	1: 43,993,870 (GRCm39)	G252W	probably damaging	Het
Trf	A	G	9: 103,100,807 (GRCm39)	F209L	probably damaging	Het
Ttn	T	C	2: 76,728,935 (GRCm39)		probably benign	Het
Uhmk1	A	T	1: 170,034,774 (GRCm39)	H311Q	probably damaging	Het
Unc13c	G	T	9: 73,840,108 (GRCm39)	Q248K	probably damaging	Het
Uqcrc2	A	G	7: 120,250,144 (GRCm39)	Y253C	probably damaging	Het
Vmn2r117	A	G	17: 23,697,390 (GRCm39)	F101L	probably benign	Het
Vmn2r43	T	C	7: 8,258,070 (GRCm39)	N381S	probably benign	Het
Zfp879	T	A	11: 50,724,024 (GRCm39)	Y271F	probably damaging	Het

Other mutations in Fryl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00819:Fryl	APN	5	73,305,451 (GRCm39)	missense	possibly damaging	0.92
IGL01518:Fryl	APN	5	73,244,305 (GRCm39)	missense	possibly damaging	0.76
IGL01545:Fryl	APN	5	73,211,940 (GRCm39)	missense	probably damaging	1.00
IGL01646:Fryl	APN	5	73,179,844 (GRCm39)	critical splice donor site	probably null
IGL01938:Fryl	APN	5	73,279,707 (GRCm39)	missense	probably damaging	0.98
IGL01962:Fryl	APN	5	73,190,134 (GRCm39)	missense	possibly damaging	0.62
IGL02064:Fryl	APN	5	73,282,112 (GRCm39)	unclassified	probably benign
IGL02148:Fryl	APN	5	73,233,302 (GRCm39)	missense	probably benign	0.35
IGL02418:Fryl	APN	5	73,267,519 (GRCm39)	splice site	probably benign
IGL02431:Fryl	APN	5	73,255,651 (GRCm39)	missense	probably benign	0.02
IGL02513:Fryl	APN	5	73,222,636 (GRCm39)	missense	probably damaging	1.00
IGL02557:Fryl	APN	5	73,255,736 (GRCm39)	missense	probably damaging	1.00
IGL02625:Fryl	APN	5	73,227,220 (GRCm39)	intron	probably benign
IGL02642:Fryl	APN	5	73,252,809 (GRCm39)	missense	probably benign
IGL02657:Fryl	APN	5	73,212,203 (GRCm39)	missense	probably benign	0.01
IGL02706:Fryl	APN	5	73,250,506 (GRCm39)	missense	probably benign	0.45
IGL03022:Fryl	APN	5	73,216,726 (GRCm39)	missense	possibly damaging	0.82
IGL03144:Fryl	APN	5	73,258,798 (GRCm39)	missense	probably null	0.22
IGL03155:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03183:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03275:Fryl	APN	5	73,305,376 (GRCm39)	missense	possibly damaging	0.47
IGL03310:Fryl	APN	5	73,293,659 (GRCm39)	splice site	probably benign
IGL03341:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03343:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03350:Fryl	APN	5	73,290,649 (GRCm39)	missense	probably damaging	0.99
IGL03357:Fryl	APN	5	73,211,402 (GRCm39)	missense	probably damaging	1.00
IGL03374:Fryl	APN	5	73,267,624 (GRCm39)	splice site	probably benign
IGL03375:Fryl	APN	5	73,245,792 (GRCm39)	missense	possibly damaging	0.91
bedeviled	UTSW	5	73,216,843 (GRCm39)	missense	probably damaging	1.00
Besotted	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R0062:Fryl	UTSW	5	73,179,621 (GRCm39)	missense	probably benign	0.02
R0062:Fryl	UTSW	5	73,179,621 (GRCm39)	missense	probably benign	0.02
R0308:Fryl	UTSW	5	73,198,947 (GRCm39)	splice site	probably benign
R0312:Fryl	UTSW	5	73,230,231 (GRCm39)	missense	probably damaging	1.00
R0415:Fryl	UTSW	5	73,255,757 (GRCm39)	missense	probably damaging	0.99
R0440:Fryl	UTSW	5	73,244,315 (GRCm39)	missense	possibly damaging	0.91
R0446:Fryl	UTSW	5	73,254,760 (GRCm39)	missense	possibly damaging	0.91
R0566:Fryl	UTSW	5	73,221,840 (GRCm39)	splice site	probably benign
R0567:Fryl	UTSW	5	73,222,734 (GRCm39)	missense	possibly damaging	0.50
R0606:Fryl	UTSW	5	73,282,077 (GRCm39)	missense	probably benign	0.15
R0619:Fryl	UTSW	5	73,226,074 (GRCm39)	missense	probably benign	0.22
R0654:Fryl	UTSW	5	73,240,715 (GRCm39)	missense	probably benign	0.17
R0658:Fryl	UTSW	5	73,222,702 (GRCm39)	missense	probably damaging	1.00
R0707:Fryl	UTSW	5	73,240,715 (GRCm39)	missense	probably benign	0.17
R0744:Fryl	UTSW	5	73,246,424 (GRCm39)	unclassified	probably benign
R0745:Fryl	UTSW	5	73,228,469 (GRCm39)	missense	probably damaging	0.96
R0833:Fryl	UTSW	5	73,246,424 (GRCm39)	unclassified	probably benign
R0885:Fryl	UTSW	5	73,246,539 (GRCm39)	missense	probably damaging	0.97
R0894:Fryl	UTSW	5	73,198,675 (GRCm39)	splice site	probably benign
R1076:Fryl	UTSW	5	73,282,016 (GRCm39)	unclassified	probably benign
R1241:Fryl	UTSW	5	73,267,614 (GRCm39)	missense	probably damaging	1.00
R1241:Fryl	UTSW	5	73,222,268 (GRCm39)	splice site	probably benign
R1394:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R1395:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R1608:Fryl	UTSW	5	73,232,094 (GRCm39)	nonsense	probably null
R1664:Fryl	UTSW	5	73,216,778 (GRCm39)	missense	probably damaging	1.00
R1745:Fryl	UTSW	5	73,190,204 (GRCm39)	splice site	probably benign
R1937:Fryl	UTSW	5	73,290,710 (GRCm39)	missense	probably damaging	1.00
R1969:Fryl	UTSW	5	73,255,609 (GRCm39)	missense	probably benign	0.18
R1993:Fryl	UTSW	5	73,265,836 (GRCm39)	missense	probably damaging	1.00
R1994:Fryl	UTSW	5	73,265,836 (GRCm39)	missense	probably damaging	1.00
R2029:Fryl	UTSW	5	73,179,465 (GRCm39)	nonsense	probably null
R2036:Fryl	UTSW	5	73,265,305 (GRCm39)	critical splice donor site	probably null
R2036:Fryl	UTSW	5	73,179,887 (GRCm39)	missense	probably benign
R2088:Fryl	UTSW	5	73,222,804 (GRCm39)	missense	probably benign	0.02
R2105:Fryl	UTSW	5	73,279,642 (GRCm39)	missense	probably benign
R2106:Fryl	UTSW	5	73,255,674 (GRCm39)	missense	probably damaging	1.00
R2186:Fryl	UTSW	5	73,222,318 (GRCm39)	missense	probably damaging	1.00
R2239:Fryl	UTSW	5	73,265,890 (GRCm39)	missense	probably damaging	0.99
R2256:Fryl	UTSW	5	73,230,187 (GRCm39)	missense	possibly damaging	0.47
R2257:Fryl	UTSW	5	73,230,187 (GRCm39)	missense	possibly damaging	0.47
R2280:Fryl	UTSW	5	73,198,707 (GRCm39)	missense	possibly damaging	0.47
R2281:Fryl	UTSW	5	73,198,707 (GRCm39)	missense	possibly damaging	0.47
R2911:Fryl	UTSW	5	73,207,799 (GRCm39)	missense	probably damaging	0.99
R3019:Fryl	UTSW	5	73,240,193 (GRCm39)	missense	probably benign	0.01
R3416:Fryl	UTSW	5	73,265,417 (GRCm39)	missense	possibly damaging	0.84
R3783:Fryl	UTSW	5	73,258,819 (GRCm39)	missense	probably benign
R3787:Fryl	UTSW	5	73,258,819 (GRCm39)	missense	probably benign
R3837:Fryl	UTSW	5	73,228,608 (GRCm39)	missense	probably benign	0.03
R3969:Fryl	UTSW	5	73,269,766 (GRCm39)	missense	probably damaging	0.97
R4387:Fryl	UTSW	5	73,243,903 (GRCm39)	missense	possibly damaging	0.91
R4502:Fryl	UTSW	5	73,245,740 (GRCm39)	missense	probably damaging	1.00
R4664:Fryl	UTSW	5	73,248,022 (GRCm39)	missense	possibly damaging	0.80
R4690:Fryl	UTSW	5	73,257,636 (GRCm39)	missense	probably benign
R4700:Fryl	UTSW	5	73,222,881 (GRCm39)	missense	possibly damaging	0.88
R4709:Fryl	UTSW	5	73,238,315 (GRCm39)	missense	probably benign	0.03
R4807:Fryl	UTSW	5	73,198,705 (GRCm39)	missense	probably benign	0.00
R4912:Fryl	UTSW	5	73,226,125 (GRCm39)	frame shift	probably null
R4948:Fryl	UTSW	5	73,246,473 (GRCm39)	missense	probably benign	0.08
R4959:Fryl	UTSW	5	73,192,401 (GRCm39)	missense	probably benign	0.00
R5062:Fryl	UTSW	5	73,233,236 (GRCm39)	missense	possibly damaging	0.89
R5067:Fryl	UTSW	5	73,215,098 (GRCm39)	missense	probably benign	0.13
R5071:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5072:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5073:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5074:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5139:Fryl	UTSW	5	73,248,061 (GRCm39)	missense	probably damaging	1.00
R5172:Fryl	UTSW	5	73,259,016 (GRCm39)	missense	possibly damaging	0.95
R5187:Fryl	UTSW	5	73,243,943 (GRCm39)	missense	possibly damaging	0.95
R5272:Fryl	UTSW	5	73,222,479 (GRCm39)	nonsense	probably null
R5275:Fryl	UTSW	5	73,270,134 (GRCm39)	missense	probably damaging	1.00
R5295:Fryl	UTSW	5	73,270,134 (GRCm39)	missense	probably damaging	1.00
R5344:Fryl	UTSW	5	73,262,117 (GRCm39)	missense	probably damaging	1.00
R5355:Fryl	UTSW	5	73,231,247 (GRCm39)	missense	probably damaging	1.00
R5716:Fryl	UTSW	5	73,257,808 (GRCm39)	missense	probably benign
R5778:Fryl	UTSW	5	73,230,121 (GRCm39)	missense	probably damaging	1.00
R5810:Fryl	UTSW	5	73,248,098 (GRCm39)	missense	probably benign	0.06
R5934:Fryl	UTSW	5	73,248,060 (GRCm39)	missense	probably damaging	1.00
R5948:Fryl	UTSW	5	73,254,715 (GRCm39)	critical splice donor site	probably null
R6005:Fryl	UTSW	5	73,240,638 (GRCm39)	missense	probably damaging	1.00
R6026:Fryl	UTSW	5	73,257,340 (GRCm39)	missense	probably benign	0.04
R6045:Fryl	UTSW	5	73,275,894 (GRCm39)	missense	probably damaging	0.99
R6185:Fryl	UTSW	5	73,270,131 (GRCm39)	missense	probably benign	0.43
R6247:Fryl	UTSW	5	73,222,824 (GRCm39)	missense	probably damaging	0.98
R6294:Fryl	UTSW	5	73,349,102 (GRCm39)	intron	probably benign
R6310:Fryl	UTSW	5	73,349,104 (GRCm39)	intron	probably benign
R6429:Fryl	UTSW	5	73,248,094 (GRCm39)	missense	possibly damaging	0.84
R6568:Fryl	UTSW	5	73,216,859 (GRCm39)	missense	probably damaging	1.00
R6636:Fryl	UTSW	5	73,290,655 (GRCm39)	missense	probably benign	0.01
R6664:Fryl	UTSW	5	73,289,824 (GRCm39)	missense	probably damaging	1.00
R6732:Fryl	UTSW	5	73,212,124 (GRCm39)	missense	probably damaging	1.00
R6750:Fryl	UTSW	5	73,179,575 (GRCm39)	missense	probably damaging	1.00
R6805:Fryl	UTSW	5	73,222,437 (GRCm39)	missense	probably benign	0.03
R6823:Fryl	UTSW	5	73,222,560 (GRCm39)	missense	probably damaging	0.99
R6855:Fryl	UTSW	5	73,216,843 (GRCm39)	missense	probably damaging	1.00
R6858:Fryl	UTSW	5	73,222,375 (GRCm39)	missense	probably damaging	1.00
R6868:Fryl	UTSW	5	73,226,146 (GRCm39)	missense	probably damaging	1.00
R6898:Fryl	UTSW	5	73,179,485 (GRCm39)	missense	probably damaging	0.96
R6908:Fryl	UTSW	5	73,179,554 (GRCm39)	missense	probably damaging	1.00
R6958:Fryl	UTSW	5	73,231,272 (GRCm39)	missense	possibly damaging	0.89
R6980:Fryl	UTSW	5	73,207,773 (GRCm39)	missense	probably benign	0.06
R7036:Fryl	UTSW	5	73,212,951 (GRCm39)	missense	probably benign	0.03
R7065:Fryl	UTSW	5	73,248,099 (GRCm39)	missense	probably damaging	0.96
R7097:Fryl	UTSW	5	73,231,251 (GRCm39)	missense	probably benign	0.31
R7171:Fryl	UTSW	5	73,279,653 (GRCm39)	missense	probably damaging	0.97
R7191:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R7207:Fryl	UTSW	5	73,222,438 (GRCm39)	missense	probably benign
R7236:Fryl	UTSW	5	73,265,821 (GRCm39)	missense	possibly damaging	0.66
R7334:Fryl	UTSW	5	73,204,839 (GRCm39)	splice site	probably null
R7425:Fryl	UTSW	5	73,262,091 (GRCm39)	missense	probably damaging	1.00
R7452:Fryl	UTSW	5	73,181,331 (GRCm39)	missense	probably damaging	1.00
R7479:Fryl	UTSW	5	73,254,904 (GRCm39)	missense	possibly damaging	0.71
R7535:Fryl	UTSW	5	73,255,539 (GRCm39)	missense	probably benign	0.15
R7538:Fryl	UTSW	5	73,180,019 (GRCm39)	missense	probably benign	0.09
R7544:Fryl	UTSW	5	73,238,382 (GRCm39)	missense	probably benign
R7548:Fryl	UTSW	5	73,349,105 (GRCm39)	missense	unknown
R7565:Fryl	UTSW	5	73,191,063 (GRCm39)	missense	probably benign	0.18
R7572:Fryl	UTSW	5	73,245,739 (GRCm39)	missense	possibly damaging	0.91
R7582:Fryl	UTSW	5	73,179,843 (GRCm39)	critical splice donor site	probably null
R7630:Fryl	UTSW	5	73,267,588 (GRCm39)	missense	possibly damaging	0.62
R7774:Fryl	UTSW	5	73,240,727 (GRCm39)	missense	probably benign	0.12
R7777:Fryl	UTSW	5	73,228,641 (GRCm39)	missense	probably damaging	0.98
R7917:Fryl	UTSW	5	73,211,875 (GRCm39)	missense	probably damaging	1.00
R7920:Fryl	UTSW	5	73,259,150 (GRCm39)	splice site	probably null
R8110:Fryl	UTSW	5	73,290,620 (GRCm39)	missense	probably benign	0.10
R8120:Fryl	UTSW	5	73,228,527 (GRCm39)	missense	probably benign	0.01
R8143:Fryl	UTSW	5	73,207,682 (GRCm39)	missense	probably benign	0.00
R8207:Fryl	UTSW	5	73,257,843 (GRCm39)	splice site	probably null
R8263:Fryl	UTSW	5	73,238,348 (GRCm39)	missense	probably damaging	1.00
R8350:Fryl	UTSW	5	73,226,073 (GRCm39)	missense	probably benign
R8359:Fryl	UTSW	5	73,233,276 (GRCm39)	missense	probably benign	0.39
R8387:Fryl	UTSW	5	73,293,663 (GRCm39)	critical splice donor site	probably null
R8403:Fryl	UTSW	5	73,275,790 (GRCm39)	makesense	probably null
R8450:Fryl	UTSW	5	73,226,073 (GRCm39)	missense	probably benign
R8514:Fryl	UTSW	5	73,242,699 (GRCm39)	missense	probably benign
R8536:Fryl	UTSW	5	73,257,696 (GRCm39)	missense	probably damaging	0.99
R8703:Fryl	UTSW	5	73,247,997 (GRCm39)	missense	probably damaging	0.99
R8708:Fryl	UTSW	5	73,289,905 (GRCm39)	missense	probably benign	0.01
R8783:Fryl	UTSW	5	73,226,185 (GRCm39)	missense	probably benign	0.45
R9028:Fryl	UTSW	5	73,255,609 (GRCm39)	missense	probably benign	0.18
R9045:Fryl	UTSW	5	73,182,118 (GRCm39)	missense
R9063:Fryl	UTSW	5	73,238,346 (GRCm39)	missense	possibly damaging	0.70
R9096:Fryl	UTSW	5	73,265,920 (GRCm39)	missense	probably benign	0.01
R9244:Fryl	UTSW	5	73,348,862 (GRCm39)	intron	probably benign
R9345:Fryl	UTSW	5	73,207,754 (GRCm39)	missense	probably benign
R9381:Fryl	UTSW	5	73,240,637 (GRCm39)	missense	probably benign	0.24
R9386:Fryl	UTSW	5	73,349,152 (GRCm39)	missense	unknown
R9401:Fryl	UTSW	5	73,222,563 (GRCm39)	nonsense	probably null
R9497:Fryl	UTSW	5	73,215,134 (GRCm39)	missense
R9514:Fryl	UTSW	5	73,262,115 (GRCm39)	missense	probably damaging	1.00
R9570:Fryl	UTSW	5	73,179,498 (GRCm39)	missense	probably benign	0.02
R9654:Fryl	UTSW	5	73,275,801 (GRCm39)	missense	probably benign
R9665:Fryl	UTSW	5	73,222,299 (GRCm39)	missense	probably damaging	1.00
R9685:Fryl	UTSW	5	73,216,879 (GRCm39)	missense	probably damaging	0.99
R9798:Fryl	UTSW	5	73,192,402 (GRCm39)	missense	probably benign
Z1088:Fryl	UTSW	5	73,248,081 (GRCm39)	missense	probably damaging	1.00
Z1088:Fryl	UTSW	5	73,248,052 (GRCm39)	missense	probably damaging	0.99
Z1176:Fryl	UTSW	5	73,230,180 (GRCm39)	missense	probably benign
Z1177:Fryl	UTSW	5	73,198,938 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- AGATCAATCGCCACACGTG -3'
(R):5'- TGCCCTACTCTTCTACAAAGAC -3'

Sequencing Primer
(F):5'- ACACGTGCCCACTGTTCG -3'
(R):5'- CAAATCTGTCTCGATGGGAACTG -3'

Posted On

2015-10-08