Mutagenetix > Incidental Mutation

Incidental Mutation 'R4663:Olfr727'

353044

Institutional Source

Beutler Lab

Gene Symbol

Olfr727

Ensembl Gene

ENSMUSG00000059488

Gene Name

olfactory receptor 727

Synonyms

GA_x6K02T2PMLR-5817082-5818056, MOR246-2

MMRRC Submission

041921-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R4663 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

50123186-50128746 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 50127482 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glycine at position 302 (R302G)

Ref Sequence

ENSEMBL: ENSMUSP00000149886 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079142] [ENSMUST00000215317]

AlphaFold

A2RTN7

Predicted Effect

probably benign
Transcript: ENSMUST00000079142
AA Change: R302G

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000078145
Gene: ENSMUSG00000059488
AA Change: R302G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	304	8.5e-49	PFAM
Pfam:7TM_GPCR_Srsx	36	290	1.5e-7	PFAM
Pfam:7tm_1	41	287	1.1e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000205947

Predicted Effect

probably benign
Transcript: ENSMUST00000215317
AA Change: R302G

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410089E03Rik	T	C	15: 8,218,455	V1496A	probably benign	Het
Aqr	A	T	2: 114,161,666	Y76*	probably null	Het
Armc5	C	A	7: 128,238,545	A140E	probably benign	Het
Auts2	T	C	5: 131,439,638	H947R	probably damaging	Het
Bag2	T	C	1: 33,746,993	T83A	probably damaging	Het
Bag4	C	T	8: 25,769,488	A228T	probably benign	Het
Bean1	A	G	8: 104,211,167	Y126C	probably damaging	Het
Cars	T	C	7: 143,575,960	E330G	probably damaging	Het
Ccdc40	A	G	11: 119,231,506	I45V	probably benign	Het
Cd320	G	A	17: 33,848,178	G214R	probably null	Het
Ckm	G	A	7: 19,419,494	V237M	probably damaging	Het
Cspg4	T	C	9: 56,886,676	V565A	possibly damaging	Het
Ephb6	A	G	6: 41,617,865	Y638C	probably damaging	Het
Epp13	T	G	7: 6,258,625	I35S	possibly damaging	Het
Fam196a	A	T	7: 134,899,148	Y409*	probably null	Het
Fat2	G	C	11: 55,296,213	S1269*	probably null	Het
Fbxo3	T	C	2: 104,053,475	V348A	probably damaging	Het
Gas6	G	A	8: 13,470,254	P478L	probably damaging	Het
Gm11492	T	A	11: 87,567,603	Y268N	probably damaging	Het
Herc1	T	C	9: 66,433,378	S1670P	probably damaging	Het
Hnrnpk	C	A	13: 58,394,517	R281L	probably damaging	Het
Ifih1	C	A	2: 62,609,219	C488F	probably benign	Het
Ift172	T	C	5: 31,284,215	K192E	probably benign	Het
Ighv5-9	T	A	12: 113,661,820	Q101L	probably benign	Het
Igkv3-2	G	T	6: 70,698,879	M57I	probably benign	Het
Itpr2	A	G	6: 146,373,173	F837S	probably damaging	Het
L3mbtl3	T	C	10: 26,337,817	Y237C	unknown	Het
Lats1	G	A	10: 7,712,583	C988Y	probably damaging	Het
Lgals3	T	A	14: 47,381,622		probably null	Het
Lrrc3b	G	T	14: 15,358,220	H129N	probably benign	Het
Lrriq1	T	C	10: 103,063,412	H1656R	possibly damaging	Het
Lypd6	T	G	2: 50,173,611	Y43*	probably null	Het
Mfsd7a	T	C	5: 108,442,145	M464V	probably benign	Het
Mical2	A	G	7: 112,328,677	D674G	possibly damaging	Het
Msi1	G	A	5: 115,450,275	R284Q	probably damaging	Het
Mybpc2	T	C	7: 44,505,642	E947G	probably damaging	Het
Nat14	T	A	7: 4,924,447	L206Q	probably damaging	Het
Nup88	A	T	11: 70,965,846		probably null	Het
Olfr27	A	T	9: 39,144,849	I250F	probably damaging	Het
Olfr743	A	T	14: 50,533,604	Y64F	probably damaging	Het
Pdcl	T	C	2: 37,355,766	E75G	probably damaging	Het
Phf14	A	G	6: 11,953,422	I387V	possibly damaging	Het
Phf3	G	A	1: 30,821,215	R845W	probably damaging	Het
Pm20d1	T	C	1: 131,798,602	I59T	probably damaging	Het
Prkd1	C	T	12: 50,419,848		probably null	Het
Psmb2	T	C	4: 126,677,765	L4P	probably damaging	Het
Pttg1	T	A	11: 43,424,850	K46*	probably null	Het
Rrnad1	A	G	3: 87,927,748	S82P	probably damaging	Het
Ryr2	T	C	13: 11,749,509	H1401R	possibly damaging	Het
Sh3d19	G	A	3: 86,123,263	D696N	probably benign	Het
Slc16a2	T	C	X: 103,707,979	T274A	probably benign	Het
Slc26a6	G	A	9: 108,857,907	A335T	probably damaging	Het
Slc6a5	C	A	7: 49,938,398	Y493*	probably null	Het
Slf1	A	T	13: 77,126,604	S37R	probably damaging	Het
Smoc1	G	A	12: 81,167,602	G264S	probably damaging	Het
Snapc4	T	C	2: 26,374,181	E280G	possibly damaging	Het
Snx25	G	A	8: 46,035,579	T913M	probably damaging	Het
Snx7	T	C	3: 117,800,879	T408A	probably benign	Het
Spdya	T	A	17: 71,578,344	S264R	probably benign	Het
Spg11	A	T	2: 122,098,099		probably null	Het
Suz12	T	A	11: 80,013,524	L230Q	probably damaging	Het
Szt2	A	G	4: 118,377,684		probably benign	Het
Tenm3	C	A	8: 48,235,970	R2194L	probably damaging	Het
Tmed8	T	A	12: 87,174,231	I194F	probably damaging	Het
Tmem79	T	A	3: 88,333,444	T66S	probably damaging	Het
Trappc1	T	A	11: 69,325,511	S118T	probably benign	Het
Ttn	C	T	2: 76,738,881	V27223I	probably benign	Het
Ttn	T	C	2: 76,776,495	T18024A	probably damaging	Het
Vmn2r15	C	T	5: 109,294,074	M164I	probably benign	Het
Vmn2r53	T	A	7: 12,600,974	Y253F	probably benign	Het
Wdr92	T	C	11: 17,232,853	V338A	probably benign	Het
Zfand6	C	T	7: 84,617,885	R163H	probably benign	Het

Other mutations in Olfr727

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00906:Olfr727	APN	14	50126757	missense	probably damaging	1.00
IGL01306:Olfr727	APN	14	50126582	missense	probably benign	0.00
ANU23:Olfr727	UTSW	14	50126582	missense	probably benign	0.00
R0498:Olfr727	UTSW	14	50127293	missense	probably damaging	1.00
R0574:Olfr727	UTSW	14	50126682	missense	probably damaging	1.00
R1201:Olfr727	UTSW	14	50127356	missense	probably damaging	1.00
R2112:Olfr727	UTSW	14	50126623	missense	probably damaging	1.00
R2435:Olfr727	UTSW	14	50126754	missense	probably damaging	1.00
R4238:Olfr727	UTSW	14	50127432	missense	probably benign
R4611:Olfr727	UTSW	14	50127073	missense	probably benign	0.12
R4672:Olfr727	UTSW	14	50127257	missense	probably benign	0.02
R5022:Olfr727	UTSW	14	50127012	missense	possibly damaging	0.78
R5062:Olfr727	UTSW	14	50127437	missense	probably damaging	1.00
R5924:Olfr727	UTSW	14	50126682	missense	probably damaging	1.00
R6702:Olfr727	UTSW	14	50127231	missense	probably damaging	1.00
R6703:Olfr727	UTSW	14	50127231	missense	probably damaging	1.00
R7497:Olfr727	UTSW	14	50127495	missense	probably benign	0.20
R7615:Olfr727	UTSW	14	50126989	missense	probably benign	0.07
R7798:Olfr727	UTSW	14	50127438	missense	probably damaging	1.00
R8413:Olfr727	UTSW	14	50127370	missense	probably benign	0.19
R8439:Olfr727	UTSW	14	50127147	missense	probably benign
R8841:Olfr727	UTSW	14	50127209	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATCGTTCCTCTGCTAGCATG -3'
(R):5'- GTAGTAAGCCTTTAAACAGCCCC -3'

Sequencing Primer
(F):5'- TCTGCTAGCATGGCCAAG -3'
(R):5'- ACAGCCCCACATATTAAATAACTAAC -3'

Posted On

2015-10-08