Mutagenetix > Incidental Mutation

Incidental Mutation 'R4664:Col16a1'

353076

Institutional Source

Beutler Lab

Gene Symbol

Col16a1

Ensembl Gene

ENSMUSG00000040690

Gene Name

collagen, type XVI, alpha 1

Synonyms

2700007F12Rik, A530052M23Rik, [a]1 (XVI) collagen

MMRRC Submission

041922-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.110) question?

Stock #

R4664 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

129941638-129993070 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

A to C at 129955883 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000121415 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044565] [ENSMUST00000123617] [ENSMUST00000143432] [ENSMUST00000143577]

AlphaFold

Q8BLX7

Predicted Effect

unknown
Transcript: ENSMUST00000044565
AA Change: K607Q

SMART Domains

Protein: ENSMUSP00000035802
Gene: ENSMUSG00000040690
AA Change: K607Q

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
TSPN	50	231	1.07e-68	SMART
internal_repeat_4	330	355	2.35e-7	PROSPERO
Pfam:Collagen	372	431	1.6e-8	PFAM
low complexity region	441	507	N/A	INTRINSIC
low complexity region	525	542	N/A	INTRINSIC
internal_repeat_2	546	562	2.68e-9	PROSPERO
internal_repeat_1	547	580	9.92e-10	PROSPERO
Pfam:Collagen	584	646	1.5e-9	PFAM
internal_repeat_5	662	689	6.35e-7	PROSPERO
internal_repeat_3	662	731	1.96e-8	PROSPERO
internal_repeat_7	679	695	2.06e-5	PROSPERO
internal_repeat_6	682	730	7.63e-6	PROSPERO
internal_repeat_1	685	742	9.92e-10	PROSPERO
Pfam:Collagen	796	850	3.4e-9	PFAM
internal_repeat_5	859	889	6.35e-7	PROSPERO
low complexity region	891	922	N/A	INTRINSIC
low complexity region	990	1000	N/A	INTRINSIC
Pfam:Collagen	1001	1064	1.4e-10	PFAM
low complexity region	1090	1112	N/A	INTRINSIC
internal_repeat_7	1114	1130	2.06e-5	PROSPERO
low complexity region	1132	1162	N/A	INTRINSIC
low complexity region	1171	1222	N/A	INTRINSIC
low complexity region	1230	1282	N/A	INTRINSIC
internal_repeat_2	1283	1299	2.68e-9	PROSPERO
internal_repeat_6	1287	1335	7.63e-6	PROSPERO
Pfam:Collagen	1350	1411	1.8e-9	PFAM
Pfam:Collagen	1446	1503	5.3e-10	PFAM
low complexity region	1505	1525	N/A	INTRINSIC
low complexity region	1528	1549	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000097867

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000106001

Predicted Effect

probably benign
Transcript: ENSMUST00000123617

SMART Domains

Protein: ENSMUSP00000121415
Gene: ENSMUSG00000040690

Domain	Start	End	E-Value	Type
Pfam:Collagen	60	97	1.2e-7	PFAM
low complexity region	117	127	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000143432
AA Change: K607Q

SMART Domains

Protein: ENSMUSP00000120384
Gene: ENSMUSG00000040690
AA Change: K607Q

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
TSPN	50	231	1.07e-68	SMART
internal_repeat_1	330	353	5.41e-8	PROSPERO
Pfam:Collagen	372	426	2.1e-9	PFAM
low complexity region	441	507	N/A	INTRINSIC
low complexity region	525	542	N/A	INTRINSIC
internal_repeat_1	546	569	5.41e-8	PROSPERO
internal_repeat_2	547	580	5.41e-8	PROSPERO
Pfam:Collagen	584	646	2.7e-10	PFAM
Pfam:Collagen	659	736	8.6e-8	PFAM
Pfam:Collagen	745	797	1.6e-7	PFAM
Pfam:Collagen	796	850	5.9e-10	PFAM
Pfam:Collagen	848	923	1.6e-7	PFAM
low complexity region	974	984	N/A	INTRINSIC
Pfam:Collagen	987	1045	1e-11	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000143577
AA Change: K292Q

SMART Domains

Protein: ENSMUSP00000120339
Gene: ENSMUSG00000040690
AA Change: K292Q

Domain	Start	End	E-Value	Type
internal_repeat_7	1	43	5.7e-5	PROSPERO
Pfam:Collagen	57	112	2e-9	PFAM
low complexity region	126	192	N/A	INTRINSIC
low complexity region	210	227	N/A	INTRINSIC
internal_repeat_2	231	247	1.5e-10	PROSPERO
internal_repeat_1	232	265	5.16e-11	PROSPERO
Pfam:Collagen	269	331	3.4e-10	PFAM
Pfam:Collagen	360	421	7e-11	PFAM
Pfam:Collagen	430	482	1.9e-7	PFAM
Pfam:Collagen	481	535	7.8e-10	PFAM
Pfam:Collagen	560	623	1.4e-7	PFAM
internal_repeat_9	640	665	9.73e-5	PROSPERO
low complexity region	675	685	N/A	INTRINSIC
Pfam:Collagen	686	747	2.5e-11	PFAM
Pfam:Collagen	730	802	5.2e-9	PFAM
Pfam:Collagen	783	860	9.2e-9	PFAM
low complexity region	871	922	N/A	INTRINSIC
low complexity region	930	985	N/A	INTRINSIC
internal_repeat_2	986	1002	1.5e-10	PROSPERO
internal_repeat_5	990	1038	7.88e-7	PROSPERO
low complexity region	1041	1110	N/A	INTRINSIC
Pfam:Collagen	1149	1205	1.8e-10	PFAM
Pfam:Collagen	1203	1260	1.1e-7	PFAM

Meta Mutation Damage Score

0.1708

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.2%

Validation Efficiency

96% (110/115)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the alpha chain of type XVI collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Members of this collagen family are found in association with fibril-forming collagens such as type I and II, and serve to maintain the integrity of the extracellular matrix. High levels of type XVI collagen have been found in fibroblasts and keratinocytes, and in smooth muscle and amnion. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 106 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ano2	A	G	6: 125,840,501 (GRCm39)	I391V	probably benign	Het
Apc	T	A	18: 34,431,647 (GRCm39)	L349M	probably damaging	Het
Atmin	A	G	8: 117,684,698 (GRCm39)	D786G	probably damaging	Het
Atp8a1	T	A	5: 67,919,929 (GRCm39)	D379V	possibly damaging	Het
Aurkb	A	G	11: 68,939,435 (GRCm39)	K173E	probably damaging	Het
Bak1	T	C	17: 27,241,510 (GRCm39)	I83V	possibly damaging	Het
Btbd17	A	C	11: 114,684,832 (GRCm39)	V69G	probably damaging	Het
Cacna1a	T	A	8: 85,328,396 (GRCm39)	Y1597*	probably null	Het
Camk2a	C	A	18: 61,088,696 (GRCm39)	Q167K	possibly damaging	Het
Capn1	A	T	19: 6,061,045 (GRCm39)	N253K	probably benign	Het
Ccer2	A	G	7: 28,455,928 (GRCm39)	E40G	probably benign	Het
Cdc42bpa	A	G	1: 179,972,130 (GRCm39)	T527A	probably damaging	Het
Cdkl2	T	C	5: 92,185,124 (GRCm39)	D89G	probably damaging	Het
Cep128	G	T	12: 91,263,027 (GRCm39)	R291S	probably damaging	Het
Cert1	T	C	13: 96,735,965 (GRCm39)	V175A	probably benign	Het
Chd4	A	G	6: 125,078,465 (GRCm39)	M203V	possibly damaging	Het
Chrna4	A	G	2: 180,679,286 (GRCm39)	S54P	probably damaging	Het
Cic	TGTTGCCCTC	T	7: 24,990,099 (GRCm39)		probably benign	Het
Cntn5	T	A	9: 10,144,214 (GRCm39)	I152L	possibly damaging	Het
Cntn6	A	G	6: 104,705,245 (GRCm39)	E154G	probably benign	Het
Cpvl	C	T	6: 53,908,918 (GRCm39)	E282K	probably benign	Het
Cyth2	T	C	7: 45,460,143 (GRCm39)	D183G	probably damaging	Het
Ddx47	A	T	6: 134,989,319 (GRCm39)	T48S	possibly damaging	Het
Dgkk	A	G	X: 6,794,751 (GRCm39)	D685G	probably benign	Het
Dis3l	C	T	9: 64,238,080 (GRCm39)	S29N	unknown	Het
Dlg5	T	C	14: 24,187,249 (GRCm39)	H1834R	possibly damaging	Het
Dnah10	T	C	5: 124,905,536 (GRCm39)	M4060T	possibly damaging	Het
Dock3	T	A	9: 106,870,743 (GRCm39)	N557I	possibly damaging	Het
Dync2i1	T	C	12: 116,219,831 (GRCm39)	E37G	probably damaging	Het
Eprs1	A	T	1: 185,105,273 (GRCm39)		probably benign	Het
Faim2	C	A	15: 99,422,581 (GRCm39)		probably null	Het
Faim2	T	G	15: 99,422,582 (GRCm39)	S72R	probably benign	Het
Fanca	T	C	8: 123,995,711 (GRCm39)	T1364A	probably damaging	Het
Fance	T	C	17: 28,534,636 (GRCm39)		probably benign	Het
Farsb	A	T	1: 78,420,402 (GRCm39)	H496Q	possibly damaging	Het
Fryl	C	T	5: 73,248,022 (GRCm39)	E1032K	possibly damaging	Het
Galnt14	A	T	17: 73,814,808 (GRCm39)		probably benign	Het
Gba2	A	T	4: 43,568,619 (GRCm39)		probably benign	Het
Gjb4	C	A	4: 127,245,571 (GRCm39)	K123N	probably damaging	Het
Gmcl1	G	A	6: 86,709,980 (GRCm39)	T56I	probably benign	Het
Gtf2ird1	T	A	5: 134,412,756 (GRCm39)	E55V	probably damaging	Het
Gtpbp2	T	C	17: 46,472,080 (GRCm39)	V5A	probably benign	Het
Hnrnpr	A	G	4: 136,044,486 (GRCm39)		probably benign	Het
Homer3	G	A	8: 70,742,793 (GRCm39)		probably null	Het
Hsd3b3	T	C	3: 98,649,532 (GRCm39)	S264G	probably damaging	Het
Ints8	T	C	4: 11,227,152 (GRCm39)	M574V	probably benign	Het
Kif4-ps	G	A	12: 101,115,477 (GRCm39)		noncoding transcript	Het
Klhl14	A	T	18: 21,687,765 (GRCm39)	N552K	probably benign	Het
Klhl40	A	G	9: 121,609,799 (GRCm39)	E528G	probably damaging	Het
Lactb2	T	C	1: 13,717,624 (GRCm39)	E133G	probably damaging	Het
Lratd2	A	T	15: 60,695,478 (GRCm39)	D89E	probably benign	Het
Lrrtm2	T	C	18: 35,347,310 (GRCm39)		probably null	Het
Mab21l2	T	A	3: 86,454,811 (GRCm39)	Y63F	probably benign	Het
Mbd1	T	A	18: 74,402,597 (GRCm39)	I33N	possibly damaging	Het
Mertk	G	A	2: 128,643,132 (GRCm39)	V844M	probably benign	Het
Myh11	T	C	16: 14,044,448 (GRCm39)	T652A	possibly damaging	Het
Nlrp4a	C	A	7: 26,148,943 (GRCm39)	Y183*	probably null	Het
Noa1	T	A	5: 77,447,600 (GRCm39)	T558S	probably benign	Het
Nol11	A	T	11: 107,071,826 (GRCm39)	S256T	possibly damaging	Het
Nr1d1	G	T	11: 98,662,086 (GRCm39)	R183S	possibly damaging	Het
Nrg2	G	T	18: 36,185,948 (GRCm39)	Q264K	possibly damaging	Het
Nsd3	T	A	8: 26,188,894 (GRCm39)	F1027I	probably damaging	Het
Ntrk3	T	A	7: 78,110,847 (GRCm39)	I285F	probably damaging	Het
Obox8	T	C	7: 14,066,771 (GRCm39)	N91S	possibly damaging	Het
Oga	T	C	19: 45,760,384 (GRCm39)	E258G	probably benign	Het
Orc5	C	T	5: 22,751,520 (GRCm39)	S63N	probably benign	Het
Osbpl6	C	T	2: 76,398,552 (GRCm39)	T412I	probably benign	Het
P2ry14	C	T	3: 59,022,563 (GRCm39)	C308Y	probably damaging	Het
Pacsin1	T	C	17: 27,926,038 (GRCm39)	F127L	probably damaging	Het
Pex11b	T	C	3: 96,551,151 (GRCm39)	L198P	possibly damaging	Het
Pla2g4e	A	T	2: 120,001,669 (GRCm39)	V660E	probably damaging	Het
Plxna4	C	A	6: 32,493,885 (GRCm39)	V244F	possibly damaging	Het
Polr3b	A	G	10: 84,550,233 (GRCm39)	Y981C	probably damaging	Het
Popdc2	T	A	16: 38,194,649 (GRCm39)	S357T	probably damaging	Het
Prr29	A	G	11: 106,267,159 (GRCm39)	H58R	probably damaging	Het
Pyy	T	A	11: 101,998,178 (GRCm39)	M1L	possibly damaging	Het
Rasd2	T	C	8: 75,948,556 (GRCm39)	S161P	possibly damaging	Het
Ryr3	C	T	2: 112,826,900 (GRCm39)		probably benign	Het
Sectm1a	G	A	11: 120,960,552 (GRCm39)	R88C	possibly damaging	Het
Serpinb13	C	T	1: 106,910,574 (GRCm39)	S66L	probably damaging	Het
Siglecf	A	T	7: 43,005,837 (GRCm39)	I465F	possibly damaging	Het
Sorl1	A	T	9: 41,915,347 (GRCm39)	M1294K	probably damaging	Het
Spam1	A	T	6: 24,796,661 (GRCm39)	H204L	probably benign	Het
Sspo	A	T	6: 48,450,468 (GRCm39)	N2586Y	possibly damaging	Het
Tbc1d4	A	G	14: 101,700,263 (GRCm39)		probably benign	Het
Tceanc2	A	T	4: 107,022,757 (GRCm39)	S77T	probably damaging	Het
Tedc2	A	G	17: 24,439,114 (GRCm39)		probably benign	Het
Tgm6	A	G	2: 129,979,314 (GRCm39)	D148G	probably benign	Het
Tgm6	A	T	2: 129,983,128 (GRCm39)	Q239L	probably benign	Het
Thsd7a	T	A	6: 12,337,313 (GRCm39)	T1235S	possibly damaging	Het
Thsd7a	T	A	6: 12,504,012 (GRCm39)	I381F	possibly damaging	Het
Tmc4	T	C	7: 3,674,270 (GRCm39)		probably null	Het
Tmem54	A	T	4: 129,004,704 (GRCm39)	E186D	possibly damaging	Het
Tpk1	A	T	6: 43,588,269 (GRCm39)	F32I	probably benign	Het
Trpc4ap	A	G	2: 155,514,917 (GRCm39)	I97T	probably benign	Het
Tut7	G	A	13: 59,948,413 (GRCm39)	T636I	possibly damaging	Het
Txlnb	A	G	10: 17,718,942 (GRCm39)	E591G	probably damaging	Het
Tyk2	C	T	9: 21,025,503 (GRCm39)	A741T	probably damaging	Het
Ubr4	G	A	4: 139,133,829 (GRCm39)	E742K	possibly damaging	Het
Uckl1	A	T	2: 181,216,661 (GRCm39)	S95T	possibly damaging	Het
Uevld	T	A	7: 46,587,734 (GRCm39)	D322V	probably damaging	Het
Ugt1a1	CAGAGAGAGAGAGA	CAGAGAGAGAGA	1: 88,139,706 (GRCm39)		probably benign	Het
Vmn1r238	G	T	18: 3,123,300 (GRCm39)	T38K	probably damaging	Het
Vps8	T	A	16: 21,262,938 (GRCm39)		probably null	Het
Wdr83	T	C	8: 85,806,680 (GRCm39)		probably benign	Het
Zfp619	A	G	7: 39,183,559 (GRCm39)	T51A	probably benign	Het

Other mutations in Col16a1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00501:Col16a1	APN	4	129,988,345 (GRCm39)	splice site	probably null
IGL00885:Col16a1	APN	4	129,990,703 (GRCm39)	missense	probably damaging	1.00
IGL01931:Col16a1	APN	4	129,966,634 (GRCm39)	missense	possibly damaging	0.47
IGL02142:Col16a1	APN	4	129,945,440 (GRCm39)	splice site	probably null
IGL02307:Col16a1	APN	4	129,952,802 (GRCm39)	missense	probably damaging	1.00
IGL02731:Col16a1	APN	4	129,947,323 (GRCm39)	unclassified	probably benign
IGL02742:Col16a1	APN	4	129,955,172 (GRCm39)	unclassified	probably benign
PIT4520001:Col16a1	UTSW	4	129,945,456 (GRCm39)	missense	unknown
R0127:Col16a1	UTSW	4	129,946,650 (GRCm39)	missense	probably damaging	1.00
R0131:Col16a1	UTSW	4	129,960,889 (GRCm39)	missense	unknown
R0131:Col16a1	UTSW	4	129,960,889 (GRCm39)	missense	unknown
R0132:Col16a1	UTSW	4	129,960,889 (GRCm39)	missense	unknown
R0299:Col16a1	UTSW	4	129,952,111 (GRCm39)	frame shift	probably null
R0355:Col16a1	UTSW	4	129,952,206 (GRCm39)	splice site	probably benign
R0395:Col16a1	UTSW	4	129,966,902 (GRCm39)	missense	probably damaging	1.00
R0485:Col16a1	UTSW	4	129,984,290 (GRCm39)	splice site	probably benign
R0573:Col16a1	UTSW	4	129,962,268 (GRCm39)	splice site	probably benign
R1274:Col16a1	UTSW	4	129,991,594 (GRCm39)	missense	probably damaging	0.98
R1619:Col16a1	UTSW	4	129,992,733 (GRCm39)	missense	probably damaging	1.00
R1759:Col16a1	UTSW	4	129,978,062 (GRCm39)	missense	probably damaging	1.00
R1832:Col16a1	UTSW	4	129,970,850 (GRCm39)	splice site	probably null
R1861:Col16a1	UTSW	4	129,955,517 (GRCm39)	unclassified	probably benign
R1862:Col16a1	UTSW	4	129,986,575 (GRCm39)	critical splice donor site	probably null
R1981:Col16a1	UTSW	4	129,959,236 (GRCm39)	missense	unknown
R2265:Col16a1	UTSW	4	129,946,711 (GRCm39)	missense	probably benign	0.02
R2269:Col16a1	UTSW	4	129,946,711 (GRCm39)	missense	probably benign	0.02
R2291:Col16a1	UTSW	4	129,960,833 (GRCm39)	missense	unknown
R3176:Col16a1	UTSW	4	129,951,792 (GRCm39)	missense	probably damaging	0.99
R3276:Col16a1	UTSW	4	129,951,792 (GRCm39)	missense	probably damaging	0.99
R3552:Col16a1	UTSW	4	129,970,834 (GRCm39)	missense	probably benign	0.10
R4049:Col16a1	UTSW	4	129,962,545 (GRCm39)	missense	probably damaging	1.00
R4241:Col16a1	UTSW	4	129,992,843 (GRCm39)	missense	probably damaging	0.98
R4327:Col16a1	UTSW	4	129,988,344 (GRCm39)	critical splice donor site	probably null
R4591:Col16a1	UTSW	4	129,955,592 (GRCm39)	splice site	probably null
R4803:Col16a1	UTSW	4	129,948,901 (GRCm39)	unclassified	probably benign
R4925:Col16a1	UTSW	4	129,947,969 (GRCm39)	missense	probably damaging	1.00
R4961:Col16a1	UTSW	4	129,948,272 (GRCm39)	splice site	probably null
R5016:Col16a1	UTSW	4	129,972,988 (GRCm39)	missense	probably benign	0.31
R5027:Col16a1	UTSW	4	129,972,988 (GRCm39)	missense	probably benign	0.31
R5085:Col16a1	UTSW	4	129,947,964 (GRCm39)	missense	probably damaging	1.00
R5088:Col16a1	UTSW	4	129,972,988 (GRCm39)	missense	probably benign	0.31
R5089:Col16a1	UTSW	4	129,972,988 (GRCm39)	missense	probably benign	0.31
R5408:Col16a1	UTSW	4	129,986,898 (GRCm39)	utr 3 prime	probably benign
R5472:Col16a1	UTSW	4	129,986,564 (GRCm39)	utr 3 prime	probably benign
R5564:Col16a1	UTSW	4	129,947,151 (GRCm39)	missense	probably damaging	1.00
R5597:Col16a1	UTSW	4	129,952,097 (GRCm39)	missense	probably damaging	1.00
R5703:Col16a1	UTSW	4	129,947,092 (GRCm39)	missense	probably damaging	0.96
R6054:Col16a1	UTSW	4	129,955,515 (GRCm39)	unclassified	probably benign
R6226:Col16a1	UTSW	4	129,948,882 (GRCm39)	unclassified	probably benign
R6362:Col16a1	UTSW	4	129,959,983 (GRCm39)	missense	unknown
R6448:Col16a1	UTSW	4	129,952,781 (GRCm39)	missense	probably damaging	1.00
R6449:Col16a1	UTSW	4	129,960,486 (GRCm39)	missense	unknown
R6502:Col16a1	UTSW	4	129,949,787 (GRCm39)	missense	probably damaging	1.00
R6949:Col16a1	UTSW	4	129,953,116 (GRCm39)	missense	probably damaging	1.00
R6969:Col16a1	UTSW	4	129,986,880 (GRCm39)	utr 3 prime	probably benign
R7086:Col16a1	UTSW	4	129,946,773 (GRCm39)	splice site	probably null
R7375:Col16a1	UTSW	4	129,959,294 (GRCm39)	missense	unknown
R7703:Col16a1	UTSW	4	129,990,295 (GRCm39)	missense	unknown
R7808:Col16a1	UTSW	4	129,967,057 (GRCm39)	missense	unknown
R7904:Col16a1	UTSW	4	129,948,001 (GRCm39)	nonsense	probably null
R7936:Col16a1	UTSW	4	129,990,664 (GRCm39)	critical splice acceptor site	probably null
R7981:Col16a1	UTSW	4	129,980,347 (GRCm39)	critical splice donor site	probably null
R8161:Col16a1	UTSW	4	129,954,262 (GRCm39)	missense	unknown
R8178:Col16a1	UTSW	4	129,947,270 (GRCm39)	missense	unknown
R8266:Col16a1	UTSW	4	129,959,224 (GRCm39)	missense	unknown
R8312:Col16a1	UTSW	4	129,948,244 (GRCm39)	missense	unknown
R8714:Col16a1	UTSW	4	129,947,961 (GRCm39)	missense	unknown
R9011:Col16a1	UTSW	4	129,946,652 (GRCm39)	missense	unknown
R9088:Col16a1	UTSW	4	129,971,016 (GRCm39)	missense	unknown
RF014:Col16a1	UTSW	4	129,986,860 (GRCm39)	critical splice acceptor site	probably benign
Z1176:Col16a1	UTSW	4	129,966,671 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGCTAGGCTGCTACTTGTAAAG -3'
(R):5'- CAAATGTGAACATGGGCATCC -3'

Sequencing Primer
(F):5'- AAGTAGTAACTGTCTAGCGGTCCC -3'
(R):5'- TAGACTCTGGAGCCAGACTTC -3'

Posted On

2015-10-08