Mutagenetix > Incidental Mutation

Incidental Mutation 'R4664:Fryl'

353081

Institutional Source

Beutler Lab

Gene Symbol

Fryl

Ensembl Gene

ENSMUSG00000070733

Gene Name

FRY like transcription coactivator

Synonyms

2510002A14Rik, 2310004H21Rik, 9030227G01Rik

MMRRC Submission

041922-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.758) question?

Stock #

R4664 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

73019987-73256619 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 73090679 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Lysine at position 1032 (E1032K)

Ref Sequence

ENSEMBL: ENSMUSP00000098687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094700] [ENSMUST00000101127]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094700
AA Change: E1032K

PolyPhen 2 Score 0.803 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000092289
Gene: ENSMUSG00000070733
AA Change: E1032K

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	117	649	5.7e-176	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	896	1141	1.3e-5	PFAM
Pfam:MOR2-PAG1_mid	1145	1331	2e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1450	1.2e-5	PFAM
low complexity region	1476	1487	N/A	INTRINSIC
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1590	1660	1.1e-5	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	3.2e-15	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2002	2255	9.9e-78	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000101127
AA Change: E1032K

PolyPhen 2 Score 0.803 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000098687
Gene: ENSMUSG00000070733
AA Change: E1032K

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	116	649	3e-172	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	898	1115	7.8e-6	PFAM
Pfam:MOR2-PAG1_mid	1147	1331	9.5e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1503	1.1e-5	PFAM
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1589	1664	6e-6	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	1.6e-14	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	1998	2260	4.4e-76	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000202381

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.2%

Validation Efficiency

96% (110/115)

MGI Phenotype

PHENOTYPE: Most mice homozygous for a knock-out allele exhibit postnatal lethality and defects in kidney development; rare survivors display growth retardation, decreased body weight, and premature death associated with chronic hydronephrosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 106 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ano2	A	G	6: 125,863,538 (GRCm38)	I391V	probably benign	Het
Apc	T	A	18: 34,298,594 (GRCm38)	L349M	probably damaging	Het
Atmin	A	G	8: 116,957,959 (GRCm38)	D786G	probably damaging	Het
Atp8a1	T	A	5: 67,762,586 (GRCm38)	D379V	possibly damaging	Het
Aurkb	A	G	11: 69,048,609 (GRCm38)	K173E	probably damaging	Het
Bak1	T	C	17: 27,022,536 (GRCm38)	I83V	possibly damaging	Het
Btbd17	A	C	11: 114,794,006 (GRCm38)	V69G	probably damaging	Het
Cacna1a	T	A	8: 84,601,767 (GRCm38)	Y1597*	probably null	Het
Camk2a	C	A	18: 60,955,624 (GRCm38)	Q167K	possibly damaging	Het
Capn1	A	T	19: 6,011,015 (GRCm38)	N253K	probably benign	Het
Ccer2	A	G	7: 28,756,503 (GRCm38)	E40G	probably benign	Het
Cdc42bpa	A	G	1: 180,144,565 (GRCm38)	T527A	probably damaging	Het
Cdkl2	T	C	5: 92,037,265 (GRCm38)	D89G	probably damaging	Het
Cep128	G	T	12: 91,296,253 (GRCm38)	R291S	probably damaging	Het
Chd4	A	G	6: 125,101,502 (GRCm38)	M203V	possibly damaging	Het
Chrna4	A	G	2: 181,037,493 (GRCm38)	S54P	probably damaging	Het
Cic	TGTTGCCCTC	T	7: 25,290,674 (GRCm38)		probably benign	Het
Cntn5	T	A	9: 10,144,209 (GRCm38)	I152L	possibly damaging	Het
Cntn6	A	G	6: 104,728,284 (GRCm38)	E154G	probably benign	Het
Col16a1	A	C	4: 130,062,090 (GRCm38)		probably benign	Het
Col4a3bp	T	C	13: 96,599,457 (GRCm38)	V175A	probably benign	Het
Cpvl	C	T	6: 53,931,933 (GRCm38)	E282K	probably benign	Het
Cyth2	T	C	7: 45,810,719 (GRCm38)	D183G	probably damaging	Het
Ddx47	A	T	6: 135,012,356 (GRCm38)	T48S	possibly damaging	Het
Dgkk	A	G	X: 6,928,512 (GRCm38)	D685G	probably benign	Het
Dis3l	C	T	9: 64,330,798 (GRCm38)	S29N	unknown	Het
Dlg5	T	C	14: 24,137,181 (GRCm38)	H1834R	possibly damaging	Het
Dnah10	T	C	5: 124,828,472 (GRCm38)	M4060T	possibly damaging	Het
Dock3	T	A	9: 106,993,544 (GRCm38)	N557I	possibly damaging	Het
Eprs	A	T	1: 185,373,076 (GRCm38)		probably benign	Het
Faim2	C	A	15: 99,524,700 (GRCm38)		probably null	Het
Faim2	T	G	15: 99,524,701 (GRCm38)	S72R	probably benign	Het
Fam84b	A	T	15: 60,823,629 (GRCm38)	D89E	probably benign	Het
Fanca	T	C	8: 123,268,972 (GRCm38)	T1364A	probably damaging	Het
Fance	T	C	17: 28,315,662 (GRCm38)		probably benign	Het
Farsb	A	T	1: 78,443,765 (GRCm38)	H496Q	possibly damaging	Het
Galnt14	A	T	17: 73,507,813 (GRCm38)		probably benign	Het
Gba2	A	T	4: 43,568,619 (GRCm38)		probably benign	Het
Gjb4	C	A	4: 127,351,778 (GRCm38)	K123N	probably damaging	Het
Gmcl1	G	A	6: 86,732,998 (GRCm38)	T56I	probably benign	Het
Gtf2ird1	T	A	5: 134,383,902 (GRCm38)	E55V	probably damaging	Het
Gtpbp2	T	C	17: 46,161,154 (GRCm38)	V5A	probably benign	Het
Hnrnpr	A	G	4: 136,317,175 (GRCm38)		probably benign	Het
Homer3	G	A	8: 70,290,143 (GRCm38)		probably null	Het
Hsd3b3	T	C	3: 98,742,216 (GRCm38)	S264G	probably damaging	Het
Ints8	T	C	4: 11,227,152 (GRCm38)	M574V	probably benign	Het
Kif4-ps	G	A	12: 101,149,218 (GRCm38)		noncoding transcript	Het
Klhl14	A	T	18: 21,554,708 (GRCm38)	N552K	probably benign	Het
Klhl40	A	G	9: 121,780,733 (GRCm38)	E528G	probably damaging	Het
Lactb2	T	C	1: 13,647,400 (GRCm38)	E133G	probably damaging	Het
Lrrtm2	T	C	18: 35,214,257 (GRCm38)		probably null	Het
Mab21l2	T	A	3: 86,547,504 (GRCm38)	Y63F	probably benign	Het
Mbd1	T	A	18: 74,269,526 (GRCm38)	I33N	possibly damaging	Het
Mertk	G	A	2: 128,801,212 (GRCm38)	V844M	probably benign	Het
Mgea5	T	C	19: 45,771,945 (GRCm38)	E258G	probably benign	Het
Myh11	T	C	16: 14,226,584 (GRCm38)	T652A	possibly damaging	Het
Nlrp4a	C	A	7: 26,449,518 (GRCm38)	Y183*	probably null	Het
Noa1	T	A	5: 77,299,753 (GRCm38)	T558S	probably benign	Het
Nol11	A	T	11: 107,181,000 (GRCm38)	S256T	possibly damaging	Het
Nr1d1	G	T	11: 98,771,260 (GRCm38)	R183S	possibly damaging	Het
Nrg2	G	T	18: 36,052,895 (GRCm38)	Q264K	possibly damaging	Het
Nsd3	T	A	8: 25,698,866 (GRCm38)	F1027I	probably damaging	Het
Ntrk3	T	A	7: 78,461,099 (GRCm38)	I285F	probably damaging	Het
Obox8	T	C	7: 14,332,846 (GRCm38)	N91S	possibly damaging	Het
Orc5	C	T	5: 22,546,522 (GRCm38)	S63N	probably benign	Het
Osbpl6	C	T	2: 76,568,208 (GRCm38)	T412I	probably benign	Het
P2ry14	C	T	3: 59,115,142 (GRCm38)	C308Y	probably damaging	Het
Pacsin1	T	C	17: 27,707,064 (GRCm38)	F127L	probably damaging	Het
Pex11b	T	C	3: 96,643,835 (GRCm38)	L198P	possibly damaging	Het
Pla2g4e	A	T	2: 120,171,188 (GRCm38)	V660E	probably damaging	Het
Plxna4	C	A	6: 32,516,950 (GRCm38)	V244F	possibly damaging	Het
Polr3b	A	G	10: 84,714,369 (GRCm38)	Y981C	probably damaging	Het
Popdc2	T	A	16: 38,374,287 (GRCm38)	S357T	probably damaging	Het
Prr29	A	G	11: 106,376,333 (GRCm38)	H58R	probably damaging	Het
Pyy	T	A	11: 102,107,352 (GRCm38)	M1L	possibly damaging	Het
Rasd2	T	C	8: 75,221,928 (GRCm38)	S161P	possibly damaging	Het
Ryr3	C	T	2: 112,996,555 (GRCm38)		probably benign	Het
Sectm1a	G	A	11: 121,069,726 (GRCm38)	R88C	possibly damaging	Het
Serpinb13	C	T	1: 106,982,844 (GRCm38)	S66L	probably damaging	Het
Siglecf	A	T	7: 43,356,413 (GRCm38)	I465F	possibly damaging	Het
Sorl1	A	T	9: 42,004,051 (GRCm38)	M1294K	probably damaging	Het
Spam1	A	T	6: 24,796,662 (GRCm38)	H204L	probably benign	Het
Sspo	A	T	6: 48,473,534 (GRCm38)	N2586Y	possibly damaging	Het
Tbc1d4	A	G	14: 101,462,827 (GRCm38)		probably benign	Het
Tceanc2	A	T	4: 107,165,560 (GRCm38)	S77T	probably damaging	Het
Tedc2	A	G	17: 24,220,140 (GRCm38)		probably benign	Het
Tgm6	A	T	2: 130,141,208 (GRCm38)	Q239L	probably benign	Het
Tgm6	A	G	2: 130,137,394 (GRCm38)	D148G	probably benign	Het
Thsd7a	T	A	6: 12,337,314 (GRCm38)	T1235S	possibly damaging	Het
Thsd7a	T	A	6: 12,504,013 (GRCm38)	I381F	possibly damaging	Het
Tmc4	T	C	7: 3,671,271 (GRCm38)		probably null	Het
Tmem54	A	T	4: 129,110,911 (GRCm38)	E186D	possibly damaging	Het
Tpk1	A	T	6: 43,611,335 (GRCm38)	F32I	probably benign	Het
Trpc4ap	A	G	2: 155,672,997 (GRCm38)	I97T	probably benign	Het
Txlnb	A	G	10: 17,843,194 (GRCm38)	E591G	probably damaging	Het
Tyk2	C	T	9: 21,114,207 (GRCm38)	A741T	probably damaging	Het
Ubr4	G	A	4: 139,406,518 (GRCm38)	E742K	possibly damaging	Het
Uckl1	A	T	2: 181,574,868 (GRCm38)	S95T	possibly damaging	Het
Uevld	T	A	7: 46,937,986 (GRCm38)	D322V	probably damaging	Het
Ugt1a1	CAGAGAGAGAGAGA	CAGAGAGAGAGA	1: 88,211,984 (GRCm38)		probably benign	Het
Vmn1r238	G	T	18: 3,123,300 (GRCm38)	T38K	probably damaging	Het
Vps8	T	A	16: 21,444,188 (GRCm38)		probably null	Het
Wdr60	T	C	12: 116,256,211 (GRCm38)	E37G	probably damaging	Het
Wdr83	T	C	8: 85,080,051 (GRCm38)		probably benign	Het
Zcchc6	G	A	13: 59,800,599 (GRCm38)	T636I	possibly damaging	Het
Zfp619	A	G	7: 39,534,135 (GRCm38)	T51A	probably benign	Het

Other mutations in Fryl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00819:Fryl	APN	5	73,148,108 (GRCm38)	missense	possibly damaging	0.92
IGL01518:Fryl	APN	5	73,086,962 (GRCm38)	missense	possibly damaging	0.76
IGL01545:Fryl	APN	5	73,054,597 (GRCm38)	missense	probably damaging	1.00
IGL01646:Fryl	APN	5	73,022,501 (GRCm38)	critical splice donor site	probably null
IGL01938:Fryl	APN	5	73,122,364 (GRCm38)	missense	probably damaging	0.98
IGL01962:Fryl	APN	5	73,032,791 (GRCm38)	missense	possibly damaging	0.62
IGL02064:Fryl	APN	5	73,124,769 (GRCm38)	unclassified	probably benign
IGL02148:Fryl	APN	5	73,075,959 (GRCm38)	missense	probably benign	0.35
IGL02418:Fryl	APN	5	73,110,176 (GRCm38)	splice site	probably benign
IGL02431:Fryl	APN	5	73,098,308 (GRCm38)	missense	probably benign	0.02
IGL02513:Fryl	APN	5	73,065,293 (GRCm38)	missense	probably damaging	1.00
IGL02557:Fryl	APN	5	73,098,393 (GRCm38)	missense	probably damaging	1.00
IGL02625:Fryl	APN	5	73,069,877 (GRCm38)	intron	probably benign
IGL02642:Fryl	APN	5	73,095,466 (GRCm38)	missense	probably benign
IGL02657:Fryl	APN	5	73,054,860 (GRCm38)	missense	probably benign	0.01
IGL02706:Fryl	APN	5	73,093,163 (GRCm38)	missense	probably benign	0.45
IGL03022:Fryl	APN	5	73,059,383 (GRCm38)	missense	possibly damaging	0.82
IGL03144:Fryl	APN	5	73,101,455 (GRCm38)	missense	probably null	0.22
IGL03155:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03183:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03275:Fryl	APN	5	73,148,033 (GRCm38)	missense	possibly damaging	0.47
IGL03310:Fryl	APN	5	73,136,316 (GRCm38)	splice site	probably benign
IGL03341:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03343:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03350:Fryl	APN	5	73,133,306 (GRCm38)	missense	probably damaging	0.99
IGL03357:Fryl	APN	5	73,054,059 (GRCm38)	missense	probably damaging	1.00
IGL03374:Fryl	APN	5	73,110,281 (GRCm38)	splice site	probably benign
IGL03375:Fryl	APN	5	73,088,449 (GRCm38)	missense	possibly damaging	0.91
bedeviled	UTSW	5	73,059,500 (GRCm38)	missense	probably damaging	1.00
Besotted	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R0062:Fryl	UTSW	5	73,022,278 (GRCm38)	missense	probably benign	0.02
R0062:Fryl	UTSW	5	73,022,278 (GRCm38)	missense	probably benign	0.02
R0308:Fryl	UTSW	5	73,041,604 (GRCm38)	splice site	probably benign
R0312:Fryl	UTSW	5	73,072,888 (GRCm38)	missense	probably damaging	1.00
R0415:Fryl	UTSW	5	73,098,414 (GRCm38)	missense	probably damaging	0.99
R0440:Fryl	UTSW	5	73,086,972 (GRCm38)	missense	possibly damaging	0.91
R0446:Fryl	UTSW	5	73,097,417 (GRCm38)	missense	possibly damaging	0.91
R0566:Fryl	UTSW	5	73,064,497 (GRCm38)	splice site	probably benign
R0567:Fryl	UTSW	5	73,065,391 (GRCm38)	missense	possibly damaging	0.50
R0606:Fryl	UTSW	5	73,124,734 (GRCm38)	missense	probably benign	0.15
R0619:Fryl	UTSW	5	73,068,731 (GRCm38)	missense	probably benign	0.22
R0654:Fryl	UTSW	5	73,083,372 (GRCm38)	missense	probably benign	0.17
R0658:Fryl	UTSW	5	73,065,359 (GRCm38)	missense	probably damaging	1.00
R0707:Fryl	UTSW	5	73,083,372 (GRCm38)	missense	probably benign	0.17
R0744:Fryl	UTSW	5	73,089,081 (GRCm38)	unclassified	probably benign
R0745:Fryl	UTSW	5	73,071,126 (GRCm38)	missense	probably damaging	0.96
R0833:Fryl	UTSW	5	73,089,081 (GRCm38)	unclassified	probably benign
R0885:Fryl	UTSW	5	73,089,196 (GRCm38)	missense	probably damaging	0.97
R0894:Fryl	UTSW	5	73,041,332 (GRCm38)	splice site	probably benign
R1076:Fryl	UTSW	5	73,124,673 (GRCm38)	unclassified	probably benign
R1241:Fryl	UTSW	5	73,110,271 (GRCm38)	missense	probably damaging	1.00
R1241:Fryl	UTSW	5	73,064,925 (GRCm38)	splice site	probably benign
R1394:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R1395:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R1608:Fryl	UTSW	5	73,074,751 (GRCm38)	nonsense	probably null
R1664:Fryl	UTSW	5	73,059,435 (GRCm38)	missense	probably damaging	1.00
R1745:Fryl	UTSW	5	73,032,861 (GRCm38)	splice site	probably benign
R1937:Fryl	UTSW	5	73,133,367 (GRCm38)	missense	probably damaging	1.00
R1969:Fryl	UTSW	5	73,098,266 (GRCm38)	missense	probably benign	0.18
R1993:Fryl	UTSW	5	73,108,493 (GRCm38)	missense	probably damaging	1.00
R1994:Fryl	UTSW	5	73,108,493 (GRCm38)	missense	probably damaging	1.00
R2029:Fryl	UTSW	5	73,022,122 (GRCm38)	nonsense	probably null
R2036:Fryl	UTSW	5	73,107,962 (GRCm38)	critical splice donor site	probably null
R2036:Fryl	UTSW	5	73,022,544 (GRCm38)	missense	probably benign
R2088:Fryl	UTSW	5	73,065,461 (GRCm38)	missense	probably benign	0.02
R2105:Fryl	UTSW	5	73,122,299 (GRCm38)	missense	probably benign
R2106:Fryl	UTSW	5	73,098,331 (GRCm38)	missense	probably damaging	1.00
R2186:Fryl	UTSW	5	73,064,975 (GRCm38)	missense	probably damaging	1.00
R2239:Fryl	UTSW	5	73,108,547 (GRCm38)	missense	probably damaging	0.99
R2256:Fryl	UTSW	5	73,072,844 (GRCm38)	missense	possibly damaging	0.47
R2257:Fryl	UTSW	5	73,072,844 (GRCm38)	missense	possibly damaging	0.47
R2280:Fryl	UTSW	5	73,041,364 (GRCm38)	missense	possibly damaging	0.47
R2281:Fryl	UTSW	5	73,041,364 (GRCm38)	missense	possibly damaging	0.47
R2911:Fryl	UTSW	5	73,050,456 (GRCm38)	missense	probably damaging	0.99
R3019:Fryl	UTSW	5	73,082,850 (GRCm38)	missense	probably benign	0.01
R3416:Fryl	UTSW	5	73,108,074 (GRCm38)	missense	possibly damaging	0.84
R3783:Fryl	UTSW	5	73,101,476 (GRCm38)	missense	probably benign
R3787:Fryl	UTSW	5	73,101,476 (GRCm38)	missense	probably benign
R3837:Fryl	UTSW	5	73,071,265 (GRCm38)	missense	probably benign	0.03
R3969:Fryl	UTSW	5	73,112,423 (GRCm38)	missense	probably damaging	0.97
R4387:Fryl	UTSW	5	73,086,560 (GRCm38)	missense	possibly damaging	0.91
R4502:Fryl	UTSW	5	73,088,397 (GRCm38)	missense	probably damaging	1.00
R4658:Fryl	UTSW	5	73,081,053 (GRCm38)	missense	probably damaging	1.00
R4690:Fryl	UTSW	5	73,100,293 (GRCm38)	missense	probably benign
R4700:Fryl	UTSW	5	73,065,538 (GRCm38)	missense	possibly damaging	0.88
R4709:Fryl	UTSW	5	73,080,972 (GRCm38)	missense	probably benign	0.03
R4807:Fryl	UTSW	5	73,041,362 (GRCm38)	missense	probably benign	0.00
R4912:Fryl	UTSW	5	73,068,782 (GRCm38)	frame shift	probably null
R4948:Fryl	UTSW	5	73,089,130 (GRCm38)	missense	probably benign	0.08
R4959:Fryl	UTSW	5	73,035,058 (GRCm38)	missense	probably benign	0.00
R5062:Fryl	UTSW	5	73,075,893 (GRCm38)	missense	possibly damaging	0.89
R5067:Fryl	UTSW	5	73,057,755 (GRCm38)	missense	probably benign	0.13
R5071:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5072:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5073:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5074:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5139:Fryl	UTSW	5	73,090,718 (GRCm38)	missense	probably damaging	1.00
R5172:Fryl	UTSW	5	73,101,673 (GRCm38)	missense	possibly damaging	0.95
R5187:Fryl	UTSW	5	73,086,600 (GRCm38)	missense	possibly damaging	0.95
R5272:Fryl	UTSW	5	73,065,136 (GRCm38)	nonsense	probably null
R5275:Fryl	UTSW	5	73,112,791 (GRCm38)	missense	probably damaging	1.00
R5295:Fryl	UTSW	5	73,112,791 (GRCm38)	missense	probably damaging	1.00
R5344:Fryl	UTSW	5	73,104,774 (GRCm38)	missense	probably damaging	1.00
R5355:Fryl	UTSW	5	73,073,904 (GRCm38)	missense	probably damaging	1.00
R5716:Fryl	UTSW	5	73,100,465 (GRCm38)	missense	probably benign
R5778:Fryl	UTSW	5	73,072,778 (GRCm38)	missense	probably damaging	1.00
R5810:Fryl	UTSW	5	73,090,755 (GRCm38)	missense	probably benign	0.06
R5934:Fryl	UTSW	5	73,090,717 (GRCm38)	missense	probably damaging	1.00
R5948:Fryl	UTSW	5	73,097,372 (GRCm38)	critical splice donor site	probably null
R6005:Fryl	UTSW	5	73,083,295 (GRCm38)	missense	probably damaging	1.00
R6026:Fryl	UTSW	5	73,099,997 (GRCm38)	missense	probably benign	0.04
R6045:Fryl	UTSW	5	73,118,551 (GRCm38)	missense	probably damaging	0.99
R6185:Fryl	UTSW	5	73,112,788 (GRCm38)	missense	probably benign	0.43
R6247:Fryl	UTSW	5	73,065,481 (GRCm38)	missense	probably damaging	0.98
R6294:Fryl	UTSW	5	73,191,759 (GRCm38)	intron	probably benign
R6310:Fryl	UTSW	5	73,191,761 (GRCm38)	intron	probably benign
R6429:Fryl	UTSW	5	73,090,751 (GRCm38)	missense	possibly damaging	0.84
R6568:Fryl	UTSW	5	73,059,516 (GRCm38)	missense	probably damaging	1.00
R6636:Fryl	UTSW	5	73,133,312 (GRCm38)	missense	probably benign	0.01
R6664:Fryl	UTSW	5	73,132,481 (GRCm38)	missense	probably damaging	1.00
R6732:Fryl	UTSW	5	73,054,781 (GRCm38)	missense	probably damaging	1.00
R6750:Fryl	UTSW	5	73,022,232 (GRCm38)	missense	probably damaging	1.00
R6805:Fryl	UTSW	5	73,065,094 (GRCm38)	missense	probably benign	0.03
R6823:Fryl	UTSW	5	73,065,217 (GRCm38)	missense	probably damaging	0.99
R6855:Fryl	UTSW	5	73,059,500 (GRCm38)	missense	probably damaging	1.00
R6858:Fryl	UTSW	5	73,065,032 (GRCm38)	missense	probably damaging	1.00
R6868:Fryl	UTSW	5	73,068,803 (GRCm38)	missense	probably damaging	1.00
R6898:Fryl	UTSW	5	73,022,142 (GRCm38)	missense	probably damaging	0.96
R6908:Fryl	UTSW	5	73,022,211 (GRCm38)	missense	probably damaging	1.00
R6958:Fryl	UTSW	5	73,073,929 (GRCm38)	missense	possibly damaging	0.89
R6980:Fryl	UTSW	5	73,050,430 (GRCm38)	missense	probably benign	0.06
R7036:Fryl	UTSW	5	73,055,608 (GRCm38)	missense	probably benign	0.03
R7065:Fryl	UTSW	5	73,090,756 (GRCm38)	missense	probably damaging	0.96
R7097:Fryl	UTSW	5	73,073,908 (GRCm38)	missense	probably benign	0.31
R7171:Fryl	UTSW	5	73,122,310 (GRCm38)	missense	probably damaging	0.97
R7191:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R7207:Fryl	UTSW	5	73,065,095 (GRCm38)	missense	probably benign
R7236:Fryl	UTSW	5	73,108,478 (GRCm38)	missense	possibly damaging	0.66
R7334:Fryl	UTSW	5	73,047,496 (GRCm38)	splice site	probably null
R7425:Fryl	UTSW	5	73,104,748 (GRCm38)	missense	probably damaging	1.00
R7452:Fryl	UTSW	5	73,023,988 (GRCm38)	missense	probably damaging	1.00
R7479:Fryl	UTSW	5	73,097,561 (GRCm38)	missense	possibly damaging	0.71
R7535:Fryl	UTSW	5	73,098,196 (GRCm38)	missense	probably benign	0.15
R7538:Fryl	UTSW	5	73,022,676 (GRCm38)	missense	probably benign	0.09
R7544:Fryl	UTSW	5	73,081,039 (GRCm38)	missense	probably benign
R7548:Fryl	UTSW	5	73,191,762 (GRCm38)	missense	unknown
R7565:Fryl	UTSW	5	73,033,720 (GRCm38)	missense	probably benign	0.18
R7572:Fryl	UTSW	5	73,088,396 (GRCm38)	missense	possibly damaging	0.91
R7582:Fryl	UTSW	5	73,022,500 (GRCm38)	critical splice donor site	probably null
R7630:Fryl	UTSW	5	73,110,245 (GRCm38)	missense	possibly damaging	0.62
R7774:Fryl	UTSW	5	73,083,384 (GRCm38)	missense	probably benign	0.12
R7777:Fryl	UTSW	5	73,071,298 (GRCm38)	missense	probably damaging	0.98
R7917:Fryl	UTSW	5	73,054,532 (GRCm38)	missense	probably damaging	1.00
R7920:Fryl	UTSW	5	73,101,807 (GRCm38)	splice site	probably null
R8110:Fryl	UTSW	5	73,133,277 (GRCm38)	missense	probably benign	0.10
R8120:Fryl	UTSW	5	73,071,184 (GRCm38)	missense	probably benign	0.01
R8143:Fryl	UTSW	5	73,050,339 (GRCm38)	missense	probably benign	0.00
R8207:Fryl	UTSW	5	73,100,500 (GRCm38)	splice site	probably null
R8263:Fryl	UTSW	5	73,081,005 (GRCm38)	missense	probably damaging	1.00
R8350:Fryl	UTSW	5	73,068,730 (GRCm38)	missense	probably benign
R8359:Fryl	UTSW	5	73,075,933 (GRCm38)	missense	probably benign	0.39
R8387:Fryl	UTSW	5	73,136,320 (GRCm38)	critical splice donor site	probably null
R8403:Fryl	UTSW	5	73,118,447 (GRCm38)	makesense	probably null
R8450:Fryl	UTSW	5	73,068,730 (GRCm38)	missense	probably benign
R8514:Fryl	UTSW	5	73,085,356 (GRCm38)	missense	probably benign
R8536:Fryl	UTSW	5	73,100,353 (GRCm38)	missense	probably damaging	0.99
R8703:Fryl	UTSW	5	73,090,654 (GRCm38)	missense	probably damaging	0.99
R8708:Fryl	UTSW	5	73,132,562 (GRCm38)	missense	probably benign	0.01
R8783:Fryl	UTSW	5	73,068,842 (GRCm38)	missense	probably benign	0.45
R9028:Fryl	UTSW	5	73,098,266 (GRCm38)	missense	probably benign	0.18
R9045:Fryl	UTSW	5	73,024,775 (GRCm38)	missense
R9063:Fryl	UTSW	5	73,081,003 (GRCm38)	missense	possibly damaging	0.70
R9096:Fryl	UTSW	5	73,108,577 (GRCm38)	missense	probably benign	0.01
R9244:Fryl	UTSW	5	73,191,519 (GRCm38)	intron	probably benign
R9345:Fryl	UTSW	5	73,050,411 (GRCm38)	missense	probably benign
R9381:Fryl	UTSW	5	73,083,294 (GRCm38)	missense	probably benign	0.24
R9386:Fryl	UTSW	5	73,191,809 (GRCm38)	missense	unknown
R9401:Fryl	UTSW	5	73,065,220 (GRCm38)	nonsense	probably null
R9497:Fryl	UTSW	5	73,057,791 (GRCm38)	missense
R9514:Fryl	UTSW	5	73,104,772 (GRCm38)	missense	probably damaging	1.00
R9570:Fryl	UTSW	5	73,022,155 (GRCm38)	missense	probably benign	0.02
R9654:Fryl	UTSW	5	73,118,458 (GRCm38)	missense	probably benign
R9665:Fryl	UTSW	5	73,064,956 (GRCm38)	missense	probably damaging	1.00
R9685:Fryl	UTSW	5	73,059,536 (GRCm38)	missense	probably damaging	0.99
R9798:Fryl	UTSW	5	73,035,059 (GRCm38)	missense	probably benign
Z1088:Fryl	UTSW	5	73,090,738 (GRCm38)	missense	probably damaging	1.00
Z1088:Fryl	UTSW	5	73,090,709 (GRCm38)	missense	probably damaging	0.99
Z1176:Fryl	UTSW	5	73,072,837 (GRCm38)	missense	probably benign
Z1177:Fryl	UTSW	5	73,041,595 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- CACATTTATTTATGTGCGGGCAC -3'
(R):5'- ACAAGCACACCAGGTTTGC -3'

Sequencing Primer
(F):5'- CATGTGTGAACATGCCTCAG -3'
(R):5'- CCGCGTGTCCTTCTGATGG -3'

Posted On

2015-10-08