Mutagenetix > Incidental Mutations

Incidental Mutation 'R4710:Coro7'

353233

Institutional Source

Beutler Lab

Gene Symbol

Coro7

Ensembl Gene

ENSMUSG00000039637

Gene Name

coronin 7

Synonyms

0610011B16Rik

MMRRC Submission

042019-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R4710 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

4626133-4679777 bp(-) (GRCm38)

Type of Mutation

intron

DNA Base Change (assembly)

T to G at 4634933 bp

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000118310 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038552] [ENSMUST00000135823]

Predicted Effect

probably benign
Transcript: ENSMUST00000038552

SMART Domains

Protein: ENSMUSP00000048489
Gene: ENSMUSG00000039637

Domain	Start	End	E-Value	Type
DUF1899	3	64	6.41e-15	SMART
WD40	66	106	1.83e-7	SMART
WD40	115	154	4.13e0	SMART
WD40	157	196	1.78e-5	SMART
DUF1900	251	385	4.49e-60	SMART
low complexity region	427	456	N/A	INTRINSIC
DUF1899	463	528	1.2e-19	SMART
WD40	531	570	3.64e-2	SMART
WD40	580	620	8.55e-8	SMART
WD40	623	662	1.16e-9	SMART
low complexity region	667	679	N/A	INTRINSIC
DUF1900	718	854	6.69e-68	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127125

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130125

Predicted Effect

probably benign
Transcript: ENSMUST00000135823

SMART Domains

Protein: ENSMUSP00000118310
Gene: ENSMUSG00000039637

Domain	Start	End	E-Value	Type
DUF1899	3	64	6.41e-15	SMART
WD40	66	106	1.83e-7	SMART
WD40	115	154	4.13e0	SMART
WD40	157	196	1.78e-5	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139072

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143723

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151156

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.9%
20x: 94.5%

Validation Efficiency

99% (75/76)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele are viable and overtly normal but exhibit disruption of the Golgi apparatus. Mutant fibroblasts show increased cell spreading and cellular F-actin content, increased cell polarization and migration, and enhanced wound healing in a scratch-wound assay. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	A	T	6: 121,641,303	Q185L	probably benign	Het
Acaca	T	A	11: 84,392,337	I2243N	possibly damaging	Het
Adamts18	A	G	8: 113,706,926	S1059P	probably damaging	Het
Aox4	A	T	1: 58,255,638	K1002M	probably damaging	Het
Ap1b1	A	G	11: 5,031,664	Y524C	probably damaging	Het
Bag4	C	T	8: 25,769,488	A228T	probably benign	Het
Bpifb6	T	A	2: 153,908,516	I309N	possibly damaging	Het
Cd209b	T	C	8: 3,924,215	E99G	probably damaging	Het
Cntn2	T	A	1: 132,528,225	H185L	possibly damaging	Het
Col4a2	C	A	8: 11,409,462	P299Q	probably benign	Het
Commd3	A	G	2: 18,674,282	N106S	probably benign	Het
Ctf1	C	A	7: 127,717,080	P66Q	probably damaging	Het
Dclre1c	T	C	2: 3,440,861		probably null	Het
Dnaaf3	A	T	7: 4,526,494	L317Q	probably damaging	Het
Dnah2	A	G	11: 69,478,077	L1667P	probably damaging	Het
Dpp4	A	G	2: 62,360,315	I399T	probably benign	Het
Dysf	C	A	6: 84,097,715	D499E	probably damaging	Het
Erg	T	C	16: 95,390,034	D90G	possibly damaging	Het
F11	T	C	8: 45,250,146	Y169C	probably damaging	Het
Fabp3	C	T	4: 130,312,387	T57I	probably benign	Het
Fcgbp	A	T	7: 28,094,961	M1197L	probably benign	Het
Gimd1	T	C	3: 132,634,848	S42P	possibly damaging	Het
Gm12666	T	C	4: 92,190,975	E203G	possibly damaging	Het
Gm5526	T	A	1: 45,857,419		noncoding transcript	Het
Gnl2	T	A	4: 125,053,459	S625T	probably benign	Het
H2-Q2	A	G	17: 35,343,302	E175G	probably damaging	Het
Hmgxb3	G	A	18: 61,137,475	P926S	probably damaging	Het
Ifi213	G	A	1: 173,567,172		probably benign	Het
Inhba	T	C	13: 16,026,483	V210A	probably benign	Het
Kansl1l	G	A	1: 66,801,496	A215V	possibly damaging	Het
Kcnk1	T	A	8: 126,029,528	V263D	probably damaging	Het
Kcnk9	A	G	15: 72,512,975	I118T	probably damaging	Het
Lamb1	A	G	12: 31,282,583	I283V	probably benign	Het
Lias	A	G	5: 65,397,727	D88G	probably benign	Het
Lrrk2	G	A	15: 91,699,927	V297M	possibly damaging	Het
Maea	C	T	5: 33,368,690	R237C	probably benign	Het
Mdfi	T	A	17: 47,824,586	N73I	probably damaging	Het
Mphosph6	T	A	8: 117,801,902	M1L	probably damaging	Het
Mta2	T	A	19: 8,949,153	I486N	probably damaging	Het
Nbr1	C	T	11: 101,575,275	P769L	probably damaging	Het
Ncam2	T	A	16: 81,465,706		probably null	Het
Ndel1	A	G	11: 68,845,337	Y26H	probably damaging	Het
Neb	T	C	2: 52,260,598	R2473G	probably benign	Het
Olfr1115	T	A	2: 87,252,040	D34E	possibly damaging	Het
Olfr140	T	C	2: 90,052,150	Y58C	probably damaging	Het
Olfr187	T	A	16: 59,036,275	H154L	possibly damaging	Het
Olfr213	T	C	6: 116,540,650	Y66H	possibly damaging	Het
Olfr325	A	T	11: 58,581,722	I293F	probably damaging	Het
Olfr459	G	T	6: 41,771,508	Q264K	probably benign	Het
Park2	A	C	17: 11,854,833	Q346P	possibly damaging	Het
Prrc2b	T	A	2: 32,193,857	S236R	possibly damaging	Het
Rae1	G	T	2: 173,015,392		probably benign	Het
Rnf112	T	C	11: 61,449,831	D491G	probably damaging	Het
Rnf220	C	T	4: 117,289,214		probably benign	Het
Ryr3	G	A	2: 112,766,301	L2483F	probably damaging	Het
Sema6a	T	C	18: 47,270,683	N624S	probably benign	Het
Sgms1	C	T	19: 32,160,137	V10M	probably damaging	Het
Slc23a2	T	C	2: 132,056,709	N636S	probably benign	Het
Slc25a48	C	T	13: 56,463,566	T162I	probably damaging	Het
Smtn	G	A	11: 3,524,663	S716F	probably damaging	Het
Snrnp200	T	C	2: 127,226,133	L850P	probably damaging	Het
Sost	G	A	11: 101,966,844	P44S	probably damaging	Het
Sox18	A	G	2: 181,670,895	Y148H	probably damaging	Het
Stk-ps1	A	G	17: 36,397,670		noncoding transcript	Het
Tas2r104	T	A	6: 131,685,444	T101S	probably damaging	Het
Traf3ip2	C	T	10: 39,639,260	P345S	possibly damaging	Het
Ttn	A	G	2: 76,752,084	Y22822H	probably damaging	Het
Vmn2r4	A	G	3: 64,409,780		probably null	Het
Vmn2r53	T	A	7: 12,601,202	H177L	probably benign	Het
Zbtb20	G	T	16: 43,610,676	A517S	probably damaging	Het
Zfat	A	T	15: 68,180,282	D554E	probably benign	Het
Zfp719	C	T	7: 43,590,232	H415Y	probably damaging	Het

Other mutations in Coro7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00428:Coro7	APN	16	4634636	missense	possibly damaging	0.83
IGL00885:Coro7	APN	16	4635026	missense	probably benign	0.00
IGL02944:Coro7	APN	16	4635412	missense	probably benign	0.14
IGL03104:Coro7	APN	16	4629126	missense	probably damaging	1.00
IGL03153:Coro7	APN	16	4635382	critical splice donor site	probably null
R0022:Coro7	UTSW	16	4633304	missense	probably benign	0.01
R0022:Coro7	UTSW	16	4633304	missense	probably benign	0.01
R0071:Coro7	UTSW	16	4670527	missense	probably damaging	1.00
R0071:Coro7	UTSW	16	4670527	missense	probably damaging	1.00
R0080:Coro7	UTSW	16	4630464	missense	probably damaging	1.00
R0193:Coro7	UTSW	16	4627504	unclassified	probably benign
R0242:Coro7	UTSW	16	4630178	splice site	probably benign
R0318:Coro7	UTSW	16	4675807	missense	probably benign	0.09
R0554:Coro7	UTSW	16	4632257	missense	possibly damaging	0.63
R0666:Coro7	UTSW	16	4631911	missense	possibly damaging	0.70
R0835:Coro7	UTSW	16	4632254	missense	probably benign	0.12
R0968:Coro7	UTSW	16	4670055	splice site	probably benign
R1670:Coro7	UTSW	16	4628233	missense	possibly damaging	0.76
R1709:Coro7	UTSW	16	4634441	splice site	probably null
R1848:Coro7	UTSW	16	4630434	missense	probably damaging	0.99
R1884:Coro7	UTSW	16	4628819	unclassified	probably benign
R1935:Coro7	UTSW	16	4628732	missense	probably benign
R1937:Coro7	UTSW	16	4628732	missense	probably benign
R1939:Coro7	UTSW	16	4628732	missense	probably benign
R1967:Coro7	UTSW	16	4634889	missense	probably damaging	1.00
R1969:Coro7	UTSW	16	4633756	missense	probably benign	0.19
R1970:Coro7	UTSW	16	4633756	missense	probably benign	0.19
R3034:Coro7	UTSW	16	4632291	missense	probably damaging	0.99
R4638:Coro7	UTSW	16	4632287	missense	probably damaging	0.96
R4723:Coro7	UTSW	16	4631994	missense	probably benign	0.00
R4789:Coro7	UTSW	16	4628221	missense	probably damaging	1.00
R5493:Coro7	UTSW	16	4632487	missense	probably damaging	0.99
R5619:Coro7	UTSW	16	4676935	critical splice donor site	probably null
R5756:Coro7	UTSW	16	4632284	missense	probably damaging	0.97
R5974:Coro7	UTSW	16	4631889	missense	possibly damaging	0.83
R6010:Coro7	UTSW	16	4669956	missense	possibly damaging	0.68
R6038:Coro7	UTSW	16	4679550	critical splice donor site	probably null
R6038:Coro7	UTSW	16	4679550	critical splice donor site	probably null
R6906:Coro7	UTSW	16	4633304	missense	probably benign	0.00
R6925:Coro7	UTSW	16	4628674	critical splice donor site	probably null
R7069:Coro7	UTSW	16	4679611	start codon destroyed	probably damaging	0.99
R7326:Coro7	UTSW	16	4632048	missense	probably damaging	0.96
R7421:Coro7	UTSW	16	4668751	missense	probably benign	0.19
R7521:Coro7	UTSW	16	4631482	missense	probably benign	0.00
R7773:Coro7	UTSW	16	4632006	missense	probably damaging	1.00
R7846:Coro7	UTSW	16	4670536	missense	probably damaging	1.00
R8240:Coro7	UTSW	16	4668796	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- CTCATGGAACTCTACAGCCTG -3'
(R):5'- TGGTTGCTGCAGGTCTACAG -3'

Sequencing Primer
(F):5'- CATGGAACTCTACAGCCTGGTGAG -3'
(R):5'- TCTACAGCACATGGTCAGGTG -3'

Posted On

2015-10-21