Mutagenetix > Incidental Mutation

Incidental Mutation 'R4711:Psma5-ps'

353275

Institutional Source

Beutler Lab

Gene Symbol

Psma5-ps

Ensembl Gene

ENSMUSG00000050490

Gene Name

proteasome subunit alpha 5, pseudogene

Synonyms

Gm8394

Accession Numbers

Essential gene?

Probably essential (E-score: 0.905) question?

Stock #

R4711 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85149352-85150303 bp(+) (GRCm39)

Type of Mutation

exon

DNA Base Change (assembly)

A to G at 85149667 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000079684

SMART Domains

Protein: ENSMUSP00000078625
Gene: ENSMUSG00000050490

Domain	Start	End	E-Value	Type
Proteasome_A_N	8	30	6.32e-8	SMART
Pfam:Proteasome	31	220	5.6e-57	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atg14	G	A	14: 47,783,298 (GRCm39)	R346C	probably damaging	Het
Bin3	A	G	14: 70,366,288 (GRCm39)		probably null	Het
Ccser1	A	G	6: 61,288,910 (GRCm39)	N358D	possibly damaging	Het
Col20a1	G	C	2: 180,634,284 (GRCm39)	G83A	probably damaging	Het
Copa	T	G	1: 171,947,555 (GRCm39)	F1124V	probably damaging	Het
Cyp2c66	G	A	19: 39,151,843 (GRCm39)	R186H	possibly damaging	Het
Dmxl2	G	A	9: 54,358,208 (GRCm39)	T323M	probably benign	Het
Dner	T	C	1: 84,361,618 (GRCm39)	I664V	possibly damaging	Het
Erp29	A	G	5: 121,583,293 (GRCm39)	I211T	possibly damaging	Het
Exd1	A	G	2: 119,369,232 (GRCm39)	S128P	possibly damaging	Het
Grik4	T	A	9: 42,540,389 (GRCm39)	N264Y	probably damaging	Het
Gsdma2	T	A	11: 98,540,439 (GRCm39)	S119R	probably damaging	Het
H2ac1	C	T	13: 24,118,796 (GRCm39)	P118S	possibly damaging	Het
Ift140	T	A	17: 25,313,691 (GRCm39)		probably null	Het
Ino80c	T	C	18: 24,247,222 (GRCm39)	N59S	probably benign	Het
Maob	T	C	X: 16,582,662 (GRCm39)	T400A	probably benign	Het
Mast4	A	G	13: 103,470,627 (GRCm39)	V25A	probably benign	Het
Mr1	T	C	1: 155,012,336 (GRCm39)	T193A	probably benign	Het
Muc5b	C	A	7: 141,399,770 (GRCm39)	F414L	unknown	Het
Nat10	A	T	2: 103,578,612 (GRCm39)	C197*	probably null	Het
Nt5c1b	A	G	12: 10,420,093 (GRCm39)	K11E	probably damaging	Het
Or5k8	A	T	16: 58,645,069 (GRCm39)	M1K	probably null	Het
Or8i2	T	C	2: 86,852,370 (GRCm39)	I173V	probably damaging	Het
Pak5	A	G	2: 135,929,437 (GRCm39)	I582T	probably damaging	Het
Pcdha12	A	G	18: 37,153,976 (GRCm39)	I232V	probably benign	Het
Pde8b	T	A	13: 95,166,958 (GRCm39)	T664S	probably benign	Het
Pdik1l	G	C	4: 134,006,301 (GRCm39)	R214G	probably benign	Het
Prss37	C	T	6: 40,492,381 (GRCm39)	V157M	probably benign	Het
Ring1	T	C	17: 34,241,333 (GRCm39)	E261G	possibly damaging	Het
Sf3a3	C	A	4: 124,621,974 (GRCm39)	D371E	probably benign	Het
Spag9	T	A	11: 94,005,177 (GRCm39)		probably null	Het
Spred1	T	A	2: 117,005,866 (GRCm39)	S209R	probably benign	Het
Tas2r121	T	C	6: 132,677,853 (GRCm39)	T40A	probably benign	Het
Tbc1d5	T	C	17: 51,242,537 (GRCm39)	T187A	probably damaging	Het
Tenm2	C	T	11: 36,191,039 (GRCm39)	V311I	probably damaging	Het
Thoc2l	T	C	5: 104,667,527 (GRCm39)	V683A	probably damaging	Het
Tnrc6a	A	G	7: 122,770,301 (GRCm39)	D697G	probably damaging	Het
Trappc14	T	C	5: 138,261,167 (GRCm39)		probably benign	Het
Ttn	G	A	2: 76,660,404 (GRCm39)	A7439V	possibly damaging	Het
Wdr64	T	C	1: 175,626,795 (GRCm39)	I838T	probably damaging	Het
Wdr7	A	G	18: 63,861,536 (GRCm39)	T183A	probably benign	Het

Other mutations in Psma5-ps

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL01336:Psma5-ps	APN	10	85,150,028 (GRCm39)	exon	noncoding transcript
IGL01467:Psma5-ps	APN	10	85,149,986 (GRCm39)	exon	noncoding transcript
IGL02638:Psma5-ps	APN	10	85,149,698 (GRCm39)	exon	noncoding transcript
IGL03253:Psma5-ps	APN	10	85,149,556 (GRCm39)	exon	noncoding transcript
R1929:Psma5-ps	UTSW	10	85,149,595 (GRCm39)	exon	noncoding transcript
R2271:Psma5-ps	UTSW	10	85,149,595 (GRCm39)	exon	noncoding transcript
R2893:Psma5-ps	UTSW	10	85,149,848 (GRCm39)	exon	noncoding transcript
R4689:Psma5-ps	UTSW	10	85,150,065 (GRCm39)	exon	noncoding transcript
R5537:Psma5-ps	UTSW	10	85,149,913 (GRCm39)	exon	noncoding transcript
R5934:Psma5-ps	UTSW	10	85,150,145 (GRCm39)	exon	noncoding transcript
T0722:Psma5-ps	UTSW	10	85,149,457 (GRCm39)	exon	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- TTTCAAGTGGAATACGCCATTGAG -3'
(R):5'- GAAACAGTTGGGGTCCTTTCC -3'

Sequencing Primer
(F):5'- TGTCTAGCTGTGGAGAAG -3'
(R):5'- TGCTACTCCAAAGGGACGAGAC -3'

Posted On

2015-10-21