Incidental Mutation 'R4713:Nefh'
ID 353384
Institutional Source Beutler Lab
Gene Symbol Nefh
Ensembl Gene ENSMUSG00000020396
Gene Name neurofilament, heavy polypeptide
Synonyms NF200, NF-H, NEFH
MMRRC Submission 041601-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R4713 (G1)
Quality Score 225
Status Not validated
Chromosome 11
Chromosomal Location 4888754-4898064 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 4889656 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 988 (T988A)
Ref Sequence ENSEMBL: ENSMUSP00000091061 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000093369]
AlphaFold P19246
Predicted Effect unknown
Transcript: ENSMUST00000093369
AA Change: T988A
SMART Domains Protein: ENSMUSP00000091061
Gene: ENSMUSG00000020396
AA Change: T988A

DomainStartEndE-ValueType
low complexity region 32 46 N/A INTRINSIC
low complexity region 49 64 N/A INTRINSIC
Filament 94 410 1.45e-109 SMART
low complexity region 470 515 N/A INTRINSIC
Pfam:DUF1388 519 545 1.8e-14 PFAM
Pfam:DUF1388 536 562 5.8e-15 PFAM
Pfam:DUF1388 542 569 2.7e-12 PFAM
Pfam:DUF1388 578 611 9.7e-10 PFAM
Pfam:DUF1388 602 629 4.9e-14 PFAM
Pfam:DUF1388 608 635 4.7e-14 PFAM
Pfam:DUF1388 626 653 1.4e-13 PFAM
Pfam:DUF1388 632 659 2.5e-13 PFAM
Pfam:DUF1388 656 683 4.4e-14 PFAM
Pfam:DUF1388 680 706 1.5e-12 PFAM
Pfam:DUF1388 700 730 5e-12 PFAM
Pfam:DUF1388 728 755 7.9e-14 PFAM
Pfam:DUF1388 752 779 4.7e-14 PFAM
Pfam:DUF1388 779 800 1.9e-9 PFAM
low complexity region 816 829 N/A INTRINSIC
low complexity region 858 948 N/A INTRINSIC
low complexity region 949 968 N/A INTRINSIC
low complexity region 976 1039 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 96.7%
  • 20x: 94.0%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit decreased axon diameter and transport. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 60 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adgrb3 G T 1: 25,586,613 (GRCm39) T360K probably damaging Het
AW551984 T C 9: 39,508,449 (GRCm39) K356E probably benign Het
Bpifb2 T A 2: 153,723,113 (GRCm39) V123E probably damaging Het
Cacna1a T G 8: 85,276,143 (GRCm39) F532V probably damaging Het
Cct8 C A 16: 87,284,576 (GRCm39) E204* probably null Het
Cd163 T A 6: 124,294,577 (GRCm39) probably null Het
Cep152 C A 2: 125,429,868 (GRCm39) A685S possibly damaging Het
Chdh A G 14: 29,758,798 (GRCm39) D581G probably benign Het
Cnpy3 A C 17: 47,058,391 (GRCm39) Y77* probably null Het
Col5a3 T C 9: 20,704,870 (GRCm39) E762G unknown Het
Creb3 A G 4: 43,563,247 (GRCm39) T115A probably benign Het
Dlat G T 9: 50,555,781 (GRCm39) A412E probably benign Het
Dnah2 T C 11: 69,367,514 (GRCm39) N1789S probably damaging Het
Dzank1 C T 2: 144,333,724 (GRCm39) E370K probably benign Het
Eif3m A T 2: 104,837,184 (GRCm39) probably null Het
Gimap8 A T 6: 48,635,920 (GRCm39) M562L probably benign Het
Gprc6a T A 10: 51,507,553 (GRCm39) probably benign Het
Gsr T G 8: 34,170,347 (GRCm39) probably null Het
Gstcd A G 3: 132,688,860 (GRCm39) V630A probably damaging Het
Hip1r T C 5: 124,128,043 (GRCm39) I116T probably benign Het
Hivep3 A G 4: 119,989,000 (GRCm39) E1817G probably damaging Het
Inpp5f A C 7: 128,265,449 (GRCm39) T135P probably damaging Het
Ism2 A G 12: 87,331,801 (GRCm39) silent Het
Itga11 A G 9: 62,673,070 (GRCm39) D784G probably damaging Het
Itpr2 A G 6: 146,274,671 (GRCm39) F837S probably damaging Het
Itpr2 T C 6: 146,298,456 (GRCm39) E10G probably damaging Het
Knl1 A T 2: 118,899,618 (GRCm39) K440* probably null Het
Lonp2 T C 8: 87,439,943 (GRCm39) S648P probably damaging Het
Lrba T C 3: 86,267,175 (GRCm39) S1622P probably benign Het
Lrp2 G T 2: 69,318,310 (GRCm39) A2047D probably damaging Het
Mcm3 G A 1: 20,873,801 (GRCm39) T773I probably benign Het
Mki67 A G 7: 135,297,198 (GRCm39) V2612A probably benign Het
Mnx1 C A 5: 29,683,129 (GRCm39) G49W probably damaging Het
Muc5b T A 7: 141,402,816 (GRCm39) Y673* probably null Het
Myo15a A G 11: 60,370,756 (GRCm39) H1172R probably benign Het
Myo1g T C 11: 6,466,080 (GRCm39) K363R probably null Het
Ncoa4 T A 14: 31,898,598 (GRCm39) C473S probably benign Het
Nwd2 T A 5: 63,961,803 (GRCm39) D462E probably benign Het
Or2av9 T C 11: 58,380,913 (GRCm39) T223A probably benign Het
Pira13 G T 7: 3,825,680 (GRCm39) Y396* probably null Het
Plec T C 15: 76,065,267 (GRCm39) E1466G unknown Het
Prl3d2 G T 13: 27,306,379 (GRCm39) M35I probably benign Het
Reln T A 5: 22,357,461 (GRCm39) I202F probably benign Het
Rhot1 T A 11: 80,116,428 (GRCm39) D78E probably benign Het
Rsph3b T C 17: 7,172,528 (GRCm39) probably null Het
Scn10a C T 9: 119,438,717 (GRCm39) M1717I probably damaging Het
Sema6a T A 18: 47,382,363 (GRCm39) H728L possibly damaging Het
Slc26a3 G T 12: 31,507,079 (GRCm39) A345S possibly damaging Het
Slc35d2 A G 13: 64,247,097 (GRCm39) V261A possibly damaging Het
Slc49a3 T C 5: 108,589,945 (GRCm39) T486A probably damaging Het
Ssmem1 A G 6: 30,519,513 (GRCm39) D66G probably damaging Het
Sult2a8 A T 7: 14,159,402 (GRCm39) N72K probably benign Het
Tbx10 T C 19: 4,046,921 (GRCm39) L108P probably damaging Het
Tex14 T C 11: 87,427,691 (GRCm39) S48P probably damaging Het
Tmie A G 9: 110,696,596 (GRCm39) L95P probably damaging Het
Tom1l2 C G 11: 60,161,259 (GRCm39) R84P probably damaging Het
Trpm3 T A 19: 22,866,799 (GRCm39) D543E possibly damaging Het
Vipr2 A C 12: 116,043,751 (GRCm39) R49S probably benign Het
Vps8 A T 16: 21,261,189 (GRCm39) S110C probably damaging Het
Zfp791 T A 8: 85,837,597 (GRCm39) N89I probably damaging Het
Other mutations in Nefh
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02931:Nefh APN 11 4,891,356 (GRCm39) missense possibly damaging 0.71
IGL03025:Nefh APN 11 4,895,289 (GRCm39) missense probably damaging 0.99
FR4340:Nefh UTSW 11 4,891,040 (GRCm39) small insertion probably benign
FR4340:Nefh UTSW 11 4,891,038 (GRCm39) small insertion probably benign
FR4340:Nefh UTSW 11 4,891,033 (GRCm39) small insertion probably benign
R0041:Nefh UTSW 11 4,895,184 (GRCm39) missense possibly damaging 0.92
R0149:Nefh UTSW 11 4,890,799 (GRCm39) missense probably benign 0.39
R0361:Nefh UTSW 11 4,890,799 (GRCm39) missense probably benign 0.39
R0531:Nefh UTSW 11 4,890,240 (GRCm39) missense probably damaging 1.00
R1340:Nefh UTSW 11 4,891,002 (GRCm39) small insertion probably benign
R1349:Nefh UTSW 11 4,891,010 (GRCm39) small insertion probably benign
R1469:Nefh UTSW 11 4,890,066 (GRCm39) missense probably benign 0.20
R1469:Nefh UTSW 11 4,890,066 (GRCm39) missense probably benign 0.20
R1564:Nefh UTSW 11 4,889,878 (GRCm39) missense unknown
R2165:Nefh UTSW 11 4,893,872 (GRCm39) missense probably damaging 1.00
R2417:Nefh UTSW 11 4,889,479 (GRCm39) missense unknown
R2906:Nefh UTSW 11 4,890,216 (GRCm39) missense probably benign 0.15
R3750:Nefh UTSW 11 4,889,937 (GRCm39) missense probably benign 0.33
R4298:Nefh UTSW 11 4,890,066 (GRCm39) missense probably benign
R4462:Nefh UTSW 11 4,891,015 (GRCm39) missense probably damaging 0.98
R4878:Nefh UTSW 11 4,891,333 (GRCm39) missense probably damaging 0.98
R5423:Nefh UTSW 11 4,890,985 (GRCm39) missense possibly damaging 0.59
R5648:Nefh UTSW 11 4,895,233 (GRCm39) missense probably damaging 1.00
R5893:Nefh UTSW 11 4,891,323 (GRCm39) missense probably damaging 1.00
R6459:Nefh UTSW 11 4,889,551 (GRCm39) missense unknown
R7583:Nefh UTSW 11 4,891,089 (GRCm39) missense probably damaging 0.96
R8557:Nefh UTSW 11 4,891,233 (GRCm39) missense probably damaging 0.98
R8925:Nefh UTSW 11 4,890,530 (GRCm39) small deletion probably benign
R8982:Nefh UTSW 11 4,897,549 (GRCm39) missense probably damaging 1.00
R9101:Nefh UTSW 11 4,890,925 (GRCm39) missense probably damaging 0.97
R9291:Nefh UTSW 11 4,890,871 (GRCm39) missense probably benign 0.39
R9576:Nefh UTSW 11 4,891,222 (GRCm39) missense possibly damaging 0.91
R9616:Nefh UTSW 11 4,889,443 (GRCm39) nonsense probably null
R9709:Nefh UTSW 11 4,890,042 (GRCm39) missense probably benign 0.44
R9781:Nefh UTSW 11 4,895,271 (GRCm39) missense probably damaging 1.00
RF001:Nefh UTSW 11 4,891,030 (GRCm39) small insertion probably benign
RF002:Nefh UTSW 11 4,891,050 (GRCm39) small insertion probably benign
RF002:Nefh UTSW 11 4,891,047 (GRCm39) small insertion probably benign
RF009:Nefh UTSW 11 4,890,997 (GRCm39) small insertion probably benign
RF012:Nefh UTSW 11 4,891,055 (GRCm39) small insertion probably benign
RF012:Nefh UTSW 11 4,891,030 (GRCm39) small insertion probably benign
RF012:Nefh UTSW 11 4,891,032 (GRCm39) small insertion probably benign
RF013:Nefh UTSW 11 4,891,032 (GRCm39) small insertion probably benign
RF016:Nefh UTSW 11 4,891,023 (GRCm39) small insertion probably benign
RF016:Nefh UTSW 11 4,891,022 (GRCm39) small insertion probably benign
RF025:Nefh UTSW 11 4,891,029 (GRCm39) small insertion probably benign
RF025:Nefh UTSW 11 4,891,003 (GRCm39) small insertion probably benign
RF028:Nefh UTSW 11 4,891,029 (GRCm39) small insertion probably benign
RF028:Nefh UTSW 11 4,891,012 (GRCm39) small insertion probably benign
RF033:Nefh UTSW 11 4,891,039 (GRCm39) small insertion probably benign
RF033:Nefh UTSW 11 4,891,029 (GRCm39) frame shift probably null
RF035:Nefh UTSW 11 4,891,039 (GRCm39) small insertion probably benign
RF036:Nefh UTSW 11 4,891,048 (GRCm39) small insertion probably benign
RF036:Nefh UTSW 11 4,891,036 (GRCm39) small insertion probably benign
RF036:Nefh UTSW 11 4,891,016 (GRCm39) small insertion probably benign
RF036:Nefh UTSW 11 4,891,010 (GRCm39) small insertion probably benign
RF037:Nefh UTSW 11 4,891,054 (GRCm39) small insertion probably benign
RF037:Nefh UTSW 11 4,891,046 (GRCm39) small insertion probably benign
RF037:Nefh UTSW 11 4,890,999 (GRCm39) small insertion probably benign
RF038:Nefh UTSW 11 4,891,019 (GRCm39) small insertion probably benign
RF038:Nefh UTSW 11 4,891,018 (GRCm39) small insertion probably benign
RF038:Nefh UTSW 11 4,891,012 (GRCm39) small insertion probably benign
RF038:Nefh UTSW 11 4,891,040 (GRCm39) small insertion probably benign
RF038:Nefh UTSW 11 4,891,029 (GRCm39) small insertion probably benign
RF038:Nefh UTSW 11 4,891,027 (GRCm39) small insertion probably benign
RF039:Nefh UTSW 11 4,891,007 (GRCm39) small insertion probably benign
RF041:Nefh UTSW 11 4,891,039 (GRCm39) small insertion probably benign
RF043:Nefh UTSW 11 4,891,016 (GRCm39) small insertion probably benign
RF044:Nefh UTSW 11 4,891,023 (GRCm39) small insertion probably benign
RF044:Nefh UTSW 11 4,891,021 (GRCm39) small insertion probably benign
RF044:Nefh UTSW 11 4,891,016 (GRCm39) small insertion probably benign
RF047:Nefh UTSW 11 4,891,038 (GRCm39) small insertion probably benign
RF048:Nefh UTSW 11 4,891,007 (GRCm39) small insertion probably benign
RF048:Nefh UTSW 11 4,891,003 (GRCm39) small insertion probably benign
RF049:Nefh UTSW 11 4,890,997 (GRCm39) small insertion probably benign
RF051:Nefh UTSW 11 4,891,054 (GRCm39) small insertion probably benign
RF053:Nefh UTSW 11 4,891,014 (GRCm39) nonsense probably null
RF054:Nefh UTSW 11 4,891,048 (GRCm39) small insertion probably benign
RF055:Nefh UTSW 11 4,891,004 (GRCm39) small insertion probably benign
RF058:Nefh UTSW 11 4,891,021 (GRCm39) small insertion probably benign
RF060:Nefh UTSW 11 4,891,052 (GRCm39) small insertion probably benign
RF060:Nefh UTSW 11 4,891,050 (GRCm39) small insertion probably benign
RF062:Nefh UTSW 11 4,891,028 (GRCm39) small insertion probably benign
T0975:Nefh UTSW 11 4,890,151 (GRCm39) missense probably benign 0.00
Z1186:Nefh UTSW 11 4,890,530 (GRCm39) small deletion probably benign
Predicted Primers PCR Primer
(F):5'- ACCTTGGCCTCCATTTTGGG -3'
(R):5'- AGGTGAAGTCCCCTGTGAAG -3'

Sequencing Primer
(F):5'- TGGGCTTCTCCTCAGGC -3'
(R):5'- TCCCCTGTGAAGGAGGAG -3'
Posted On 2015-10-21