Incidental Mutation 'R4684:Eps8l1'
ID |
353515 |
Institutional Source |
Beutler Lab
|
Gene Symbol |
Eps8l1
|
Ensembl Gene |
ENSMUSG00000006154 |
Gene Name |
EPS8-like 1 |
Synonyms |
4632407K17Rik, 2310051G19Rik, DRC3, EPS8R1 |
Accession Numbers |
|
Essential gene? |
Non essential
(E-score: 0.000)
|
Stock # |
R4684 (G1)
|
Quality Score |
225 |
Status
|
Not validated
|
Chromosome |
7 |
Chromosomal Location |
4460674-4480487 bp(+) (GRCm38) |
Type of Mutation |
missense |
DNA Base Change (assembly) |
C to A
at 4473945 bp (GRCm38)
|
Zygosity |
Heterozygous |
Amino Acid Change |
Proline to Glutamine
at position 471
(P471Q)
|
Ref Sequence |
ENSEMBL: ENSMUSP00000133206
(fasta)
|
Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000086372]
[ENSMUST00000163137]
[ENSMUST00000163804]
[ENSMUST00000163893]
[ENSMUST00000164987]
[ENSMUST00000167298]
[ENSMUST00000167810]
[ENSMUST00000169820]
[ENSMUST00000171445]
[ENSMUST00000170635]
|
AlphaFold |
Q8R5F8 |
Predicted Effect |
probably benign
Transcript: ENSMUST00000086372
AA Change: P410Q
PolyPhen 2
Score 0.087 (Sensitivity: 0.93; Specificity: 0.85)
|
SMART Domains |
Protein: ENSMUSP00000083559 Gene: ENSMUSG00000006154 AA Change: P410Q
Domain | Start | End | E-Value | Type |
Pfam:PTB
|
35 |
165 |
2.1e-46 |
PFAM |
low complexity region
|
282 |
304 |
N/A |
INTRINSIC |
SH3
|
480 |
535 |
2.62e-11 |
SMART |
low complexity region
|
554 |
564 |
N/A |
INTRINSIC |
PDB:1WWU|A
|
632 |
698 |
1e-19 |
PDB |
low complexity region
|
701 |
715 |
N/A |
INTRINSIC |
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000146199
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000163137
|
SMART Domains |
Protein: ENSMUSP00000131345 Gene: ENSMUSG00000006154
Domain | Start | End | E-Value | Type |
Pfam:PTB
|
35 |
100 |
1.9e-22 |
PFAM |
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000163804
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000163893
AA Change: P410Q
PolyPhen 2
Score 0.087 (Sensitivity: 0.93; Specificity: 0.85)
|
SMART Domains |
Protein: ENSMUSP00000125840 Gene: ENSMUSG00000006154 AA Change: P410Q
Domain | Start | End | E-Value | Type |
Pfam:PTB
|
35 |
165 |
2.1e-46 |
PFAM |
low complexity region
|
282 |
304 |
N/A |
INTRINSIC |
SH3
|
480 |
535 |
2.62e-11 |
SMART |
low complexity region
|
554 |
564 |
N/A |
INTRINSIC |
PDB:1WWU|A
|
632 |
698 |
1e-19 |
PDB |
low complexity region
|
701 |
715 |
N/A |
INTRINSIC |
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000164987
|
SMART Domains |
Protein: ENSMUSP00000130665 Gene: ENSMUSG00000006154
Domain | Start | End | E-Value | Type |
low complexity region
|
15 |
25 |
N/A |
INTRINSIC |
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000167068
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000167298
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000167599
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000167810
|
SMART Domains |
Protein: ENSMUSP00000126720 Gene: ENSMUSG00000006154
Domain | Start | End | E-Value | Type |
Pfam:PTB
|
35 |
152 |
5e-44 |
PFAM |
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000168924
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000169820
|
SMART Domains |
Protein: ENSMUSP00000131773 Gene: ENSMUSG00000006154
Domain | Start | End | E-Value | Type |
Pfam:PTB
|
35 |
93 |
1.1e-21 |
PFAM |
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000170423
|
Predicted Effect |
probably damaging
Transcript: ENSMUST00000171445
AA Change: P471Q
PolyPhen 2
Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)
|
SMART Domains |
Protein: ENSMUSP00000133206 Gene: ENSMUSG00000006154 AA Change: P471Q
Domain | Start | End | E-Value | Type |
Pfam:PTB
|
96 |
226 |
5.8e-46 |
PFAM |
low complexity region
|
343 |
365 |
N/A |
INTRINSIC |
SH3
|
541 |
596 |
2.62e-11 |
SMART |
low complexity region
|
615 |
625 |
N/A |
INTRINSIC |
PDB:1WWU|A
|
693 |
759 |
1e-19 |
PDB |
low complexity region
|
762 |
776 |
N/A |
INTRINSIC |
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000171665
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000170635
|
SMART Domains |
Protein: ENSMUSP00000127999 Gene: ENSMUSG00000006154
Domain | Start | End | E-Value | Type |
PDB:2CY5|A
|
26 |
52 |
3e-13 |
PDB |
|
Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.0%
- 20x: 94.7%
|
Validation Efficiency |
|
MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that is related to epidermal growth factor receptor pathway substrate 8 (EPS8), a substrate for the epidermal growth factor receptor. The function of this protein is unknown. At least two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
|
Allele List at MGI |
|
Other mutations in this stock |
Total: 89 list
Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
2310039H08Rik |
T |
C |
17: 46,772,946 (GRCm38) |
V45A |
probably benign |
Het |
4921509C19Rik |
A |
G |
2: 151,471,871 (GRCm38) |
I629T |
unknown |
Het |
4933402N03Rik |
T |
C |
7: 131,138,684 (GRCm38) |
R268G |
probably damaging |
Het |
Abca13 |
A |
T |
11: 9,434,193 (GRCm38) |
R3882* |
probably null |
Het |
Adamts3 |
T |
G |
5: 89,703,007 (GRCm38) |
T558P |
probably damaging |
Het |
Ano2 |
A |
G |
6: 125,790,341 (GRCm38) |
N214S |
probably benign |
Het |
Arhgef4 |
A |
T |
1: 34,811,785 (GRCm38) |
|
probably null |
Het |
Boc |
C |
T |
16: 44,500,380 (GRCm38) |
A306T |
probably benign |
Het |
Capn10 |
T |
C |
1: 92,943,781 (GRCm38) |
F367S |
probably damaging |
Het |
Ccdc6 |
T |
C |
10: 70,189,256 (GRCm38) |
|
probably benign |
Het |
Cobll1 |
G |
T |
2: 65,099,028 (GRCm38) |
S688R |
possibly damaging |
Het |
Cpxm2 |
G |
T |
7: 132,049,038 (GRCm38) |
P631Q |
possibly damaging |
Het |
Cyp2c68 |
A |
G |
19: 39,699,335 (GRCm38) |
V406A |
possibly damaging |
Het |
Cyp4a30b |
T |
A |
4: 115,455,003 (GRCm38) |
Y118N |
probably damaging |
Het |
Dgki |
A |
T |
6: 37,299,846 (GRCm38) |
|
probably benign |
Het |
Disp2 |
A |
G |
2: 118,792,756 (GRCm38) |
N1323S |
probably damaging |
Het |
Dock1 |
T |
A |
7: 134,724,409 (GRCm38) |
Y42* |
probably null |
Het |
Fam20a |
A |
C |
11: 109,721,687 (GRCm38) |
L10R |
unknown |
Het |
Fpr-rs4 |
T |
A |
17: 18,022,184 (GRCm38) |
I151K |
probably damaging |
Het |
Gga1 |
C |
A |
15: 78,885,309 (GRCm38) |
P161T |
probably damaging |
Het |
Gm8909 |
A |
T |
17: 36,165,858 (GRCm38) |
H241Q |
possibly damaging |
Het |
Gm9923 |
T |
A |
10: 72,309,476 (GRCm38) |
Y52* |
probably null |
Het |
Gucy2g |
A |
G |
19: 55,206,256 (GRCm38) |
F910L |
probably damaging |
Het |
Helz |
T |
C |
11: 107,649,145 (GRCm38) |
V315A |
probably damaging |
Het |
Hk2 |
T |
C |
6: 82,739,648 (GRCm38) |
Y301C |
probably damaging |
Het |
Htt |
C |
T |
5: 34,852,765 (GRCm38) |
P1521S |
probably damaging |
Het |
Iah1 |
T |
C |
12: 21,316,433 (GRCm38) |
M1T |
probably null |
Het |
Ik |
T |
C |
18: 36,752,414 (GRCm38) |
S287P |
probably damaging |
Het |
Itga1 |
T |
A |
13: 115,049,370 (GRCm38) |
D32V |
probably damaging |
Het |
Itpr2 |
A |
G |
6: 146,373,173 (GRCm38) |
F837S |
probably damaging |
Het |
Klk14 |
A |
G |
7: 43,691,968 (GRCm38) |
I15V |
probably benign |
Het |
Kng2 |
T |
C |
16: 22,987,641 (GRCm38) |
I603V |
possibly damaging |
Het |
Lama1 |
T |
C |
17: 67,773,778 (GRCm38) |
I1267T |
possibly damaging |
Het |
Lrp1b |
A |
C |
2: 40,922,304 (GRCm38) |
L2430V |
probably benign |
Het |
Lrrn3 |
T |
G |
12: 41,454,244 (GRCm38) |
K25Q |
possibly damaging |
Het |
Lta4h |
T |
A |
10: 93,468,816 (GRCm38) |
N233K |
probably benign |
Het |
Mapk13 |
T |
C |
17: 28,770,049 (GRCm38) |
I53T |
probably damaging |
Het |
Mdn1 |
T |
C |
4: 32,666,430 (GRCm38) |
F123L |
probably damaging |
Het |
Myh4 |
G |
C |
11: 67,245,811 (GRCm38) |
D472H |
probably damaging |
Het |
Nipa2 |
A |
T |
7: 55,935,826 (GRCm38) |
N121K |
probably benign |
Het |
Nostrin |
C |
T |
2: 69,183,924 (GRCm38) |
T408M |
probably benign |
Het |
Oosp2 |
C |
T |
19: 11,649,653 (GRCm38) |
R102H |
probably damaging |
Het |
Or1j18 |
A |
G |
2: 36,734,674 (GRCm38) |
M118V |
probably damaging |
Het |
Or4k15b |
T |
C |
14: 50,034,830 (GRCm38) |
D191G |
probably damaging |
Het |
Osgin2 |
T |
A |
4: 16,001,946 (GRCm38) |
I202L |
probably benign |
Het |
Pbld2 |
C |
A |
10: 63,057,697 (GRCm38) |
R271S |
probably damaging |
Het |
Pex6 |
C |
T |
17: 46,712,101 (GRCm38) |
T201I |
probably benign |
Het |
Pilra |
T |
C |
5: 137,835,515 (GRCm38) |
I96M |
probably damaging |
Het |
Pllp |
T |
A |
8: 94,677,278 (GRCm38) |
D47V |
possibly damaging |
Het |
Plxna2 |
A |
G |
1: 194,762,594 (GRCm38) |
S765G |
probably benign |
Het |
Prkca |
A |
T |
11: 107,961,608 (GRCm38) |
Y100N |
probably damaging |
Het |
Prkg1 |
T |
A |
19: 31,664,179 (GRCm38) |
K35* |
probably null |
Het |
Psmc2 |
A |
G |
5: 21,803,265 (GRCm38) |
D389G |
possibly damaging |
Het |
Rnf213 |
A |
G |
11: 119,441,125 (GRCm38) |
T2387A |
probably damaging |
Het |
Ros1 |
T |
C |
10: 52,129,096 (GRCm38) |
N914S |
probably damaging |
Het |
Ruvbl1 |
C |
A |
6: 88,491,599 (GRCm38) |
T367K |
probably benign |
Het |
Scube2 |
C |
T |
7: 109,810,713 (GRCm38) |
R525H |
probably damaging |
Het |
Sec14l4 |
T |
C |
11: 4,035,200 (GRCm38) |
|
probably null |
Het |
Secisbp2l |
T |
C |
2: 125,745,942 (GRCm38) |
D751G |
probably damaging |
Het |
Setd3 |
T |
C |
12: 108,108,690 (GRCm38) |
D402G |
probably benign |
Het |
Slc15a2 |
T |
A |
16: 36,757,849 (GRCm38) |
K359N |
probably damaging |
Het |
Slc25a21 |
A |
G |
12: 57,196,936 (GRCm38) |
S2P |
probably benign |
Het |
Slfn8 |
A |
T |
11: 83,017,506 (GRCm38) |
H70Q |
probably benign |
Het |
Spef2 |
T |
C |
15: 9,647,490 (GRCm38) |
I944V |
probably benign |
Het |
Spg11 |
A |
G |
2: 122,065,076 (GRCm38) |
F1887S |
probably damaging |
Het |
Sptbn4 |
T |
C |
7: 27,364,419 (GRCm38) |
E879G |
probably damaging |
Het |
Sptbn4 |
A |
T |
7: 27,366,735 (GRCm38) |
D649E |
possibly damaging |
Het |
Stx5a |
C |
A |
19: 8,743,361 (GRCm38) |
R121S |
probably damaging |
Het |
Tbcd |
T |
C |
11: 121,493,771 (GRCm38) |
L26P |
probably damaging |
Het |
Tecpr1 |
T |
C |
5: 144,207,437 (GRCm38) |
D649G |
probably benign |
Het |
Tfam |
A |
G |
10: 71,237,847 (GRCm38) |
S32P |
probably benign |
Het |
Tmt1b |
G |
T |
10: 128,960,702 (GRCm38) |
C79* |
probably null |
Het |
Trmt44 |
C |
T |
5: 35,558,043 (GRCm38) |
R642H |
probably benign |
Het |
Trpm3 |
G |
A |
19: 22,987,781 (GRCm38) |
A1547T |
probably benign |
Het |
Ttll6 |
T |
C |
11: 96,153,177 (GRCm38) |
V519A |
probably benign |
Het |
Umodl1 |
T |
C |
17: 30,998,114 (GRCm38) |
F1107L |
probably benign |
Het |
Usp5 |
A |
T |
6: 124,817,956 (GRCm38) |
V677E |
probably damaging |
Het |
Utp20 |
A |
T |
10: 88,807,445 (GRCm38) |
L605* |
probably null |
Het |
Utrn |
T |
C |
10: 12,745,240 (GRCm38) |
D229G |
probably damaging |
Het |
Uty |
A |
T |
Y: 1,176,502 (GRCm38) |
L178* |
probably null |
Het |
Vmn2r88 |
A |
T |
14: 51,413,334 (GRCm38) |
D168V |
possibly damaging |
Het |
Vps13b |
T |
C |
15: 35,646,178 (GRCm38) |
V1476A |
probably damaging |
Het |
Vps13b |
C |
T |
15: 35,841,341 (GRCm38) |
H2506Y |
probably benign |
Het |
Vps13b |
C |
A |
15: 35,879,821 (GRCm38) |
T3014K |
probably benign |
Het |
Vps37c |
T |
C |
19: 10,712,768 (GRCm38) |
V198A |
probably benign |
Het |
Zfc3h1 |
T |
C |
10: 115,423,385 (GRCm38) |
Y1621H |
probably benign |
Het |
Zfp251 |
T |
C |
15: 76,854,407 (GRCm38) |
D162G |
possibly damaging |
Het |
Zfp292 |
T |
C |
4: 34,807,078 (GRCm38) |
T1994A |
probably benign |
Het |
Zfp791 |
A |
G |
8: 85,110,930 (GRCm38) |
Y102H |
probably benign |
Het |
|
Other mutations in Eps8l1 |
Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
IGL01292:Eps8l1
|
APN |
7 |
4,478,920 (GRCm38) |
utr 3 prime |
probably benign |
|
IGL01455:Eps8l1
|
APN |
7 |
4,478,923 (GRCm38) |
utr 3 prime |
probably benign |
|
IGL01872:Eps8l1
|
APN |
7 |
4,472,296 (GRCm38) |
splice site |
probably benign |
|
IGL02343:Eps8l1
|
APN |
7 |
4,472,124 (GRCm38) |
missense |
probably benign |
0.04 |
IGL02585:Eps8l1
|
APN |
7 |
4,469,213 (GRCm38) |
missense |
probably damaging |
1.00 |
IGL02596:Eps8l1
|
APN |
7 |
4,470,872 (GRCm38) |
missense |
probably damaging |
0.99 |
IGL02673:Eps8l1
|
APN |
7 |
4,478,732 (GRCm38) |
missense |
probably damaging |
1.00 |
IGL03117:Eps8l1
|
APN |
7 |
4,470,887 (GRCm38) |
missense |
probably damaging |
1.00 |
Anamnestic
|
UTSW |
7 |
4,470,874 (GRCm38) |
missense |
probably damaging |
0.98 |
souvenir
|
UTSW |
7 |
4,477,896 (GRCm38) |
missense |
possibly damaging |
0.56 |
PIT4142001:Eps8l1
|
UTSW |
7 |
4,471,415 (GRCm38) |
missense |
probably benign |
0.00 |
PIT4151001:Eps8l1
|
UTSW |
7 |
4,471,415 (GRCm38) |
missense |
probably benign |
0.00 |
PIT4480001:Eps8l1
|
UTSW |
7 |
4,471,415 (GRCm38) |
missense |
probably benign |
0.00 |
R0015:Eps8l1
|
UTSW |
7 |
4,477,557 (GRCm38) |
splice site |
probably benign |
|
R0599:Eps8l1
|
UTSW |
7 |
4,477,957 (GRCm38) |
missense |
possibly damaging |
0.90 |
R0686:Eps8l1
|
UTSW |
7 |
4,477,450 (GRCm38) |
missense |
probably benign |
0.36 |
R0827:Eps8l1
|
UTSW |
7 |
4,477,389 (GRCm38) |
missense |
possibly damaging |
0.86 |
R1015:Eps8l1
|
UTSW |
7 |
4,469,933 (GRCm38) |
missense |
probably damaging |
1.00 |
R1447:Eps8l1
|
UTSW |
7 |
4,474,056 (GRCm38) |
missense |
probably damaging |
1.00 |
R1490:Eps8l1
|
UTSW |
7 |
4,470,889 (GRCm38) |
missense |
probably damaging |
1.00 |
R1527:Eps8l1
|
UTSW |
7 |
4,471,394 (GRCm38) |
missense |
probably benign |
|
R1553:Eps8l1
|
UTSW |
7 |
4,477,449 (GRCm38) |
missense |
probably damaging |
0.98 |
R1763:Eps8l1
|
UTSW |
7 |
4,471,823 (GRCm38) |
missense |
probably benign |
0.43 |
R1863:Eps8l1
|
UTSW |
7 |
4,465,360 (GRCm38) |
utr 5 prime |
probably benign |
|
R2357:Eps8l1
|
UTSW |
7 |
4,470,355 (GRCm38) |
missense |
probably benign |
0.06 |
R3153:Eps8l1
|
UTSW |
7 |
4,471,799 (GRCm38) |
missense |
probably damaging |
1.00 |
R4082:Eps8l1
|
UTSW |
7 |
4,470,798 (GRCm38) |
splice site |
probably null |
|
R4539:Eps8l1
|
UTSW |
7 |
4,478,624 (GRCm38) |
missense |
probably damaging |
1.00 |
R4930:Eps8l1
|
UTSW |
7 |
4,460,916 (GRCm38) |
missense |
possibly damaging |
0.66 |
R4931:Eps8l1
|
UTSW |
7 |
4,471,241 (GRCm38) |
missense |
possibly damaging |
0.95 |
R5245:Eps8l1
|
UTSW |
7 |
4,470,874 (GRCm38) |
missense |
probably damaging |
0.98 |
R5247:Eps8l1
|
UTSW |
7 |
4,470,402 (GRCm38) |
missense |
probably damaging |
1.00 |
R5305:Eps8l1
|
UTSW |
7 |
4,477,896 (GRCm38) |
missense |
possibly damaging |
0.56 |
R5420:Eps8l1
|
UTSW |
7 |
4,470,161 (GRCm38) |
splice site |
probably null |
|
R5620:Eps8l1
|
UTSW |
7 |
4,460,946 (GRCm38) |
missense |
possibly damaging |
0.83 |
R5705:Eps8l1
|
UTSW |
7 |
4,470,035 (GRCm38) |
missense |
probably benign |
0.00 |
R6063:Eps8l1
|
UTSW |
7 |
4,471,297 (GRCm38) |
missense |
possibly damaging |
0.56 |
R6909:Eps8l1
|
UTSW |
7 |
4,469,900 (GRCm38) |
nonsense |
probably null |
|
R7096:Eps8l1
|
UTSW |
7 |
4,474,191 (GRCm38) |
missense |
probably benign |
0.01 |
R7136:Eps8l1
|
UTSW |
7 |
4,477,404 (GRCm38) |
missense |
probably damaging |
1.00 |
R7144:Eps8l1
|
UTSW |
7 |
4,472,185 (GRCm38) |
missense |
probably damaging |
1.00 |
R7381:Eps8l1
|
UTSW |
7 |
4,470,438 (GRCm38) |
splice site |
probably null |
|
R7539:Eps8l1
|
UTSW |
7 |
4,470,037 (GRCm38) |
missense |
probably damaging |
1.00 |
R7784:Eps8l1
|
UTSW |
7 |
4,472,122 (GRCm38) |
missense |
probably damaging |
1.00 |
R7833:Eps8l1
|
UTSW |
7 |
4,468,867 (GRCm38) |
missense |
possibly damaging |
0.76 |
R8190:Eps8l1
|
UTSW |
7 |
4,471,298 (GRCm38) |
missense |
probably benign |
0.05 |
R8311:Eps8l1
|
UTSW |
7 |
4,471,818 (GRCm38) |
missense |
probably damaging |
1.00 |
R8549:Eps8l1
|
UTSW |
7 |
4,470,854 (GRCm38) |
missense |
probably damaging |
1.00 |
R8960:Eps8l1
|
UTSW |
7 |
4,478,215 (GRCm38) |
missense |
probably damaging |
1.00 |
R8974:Eps8l1
|
UTSW |
7 |
4,471,818 (GRCm38) |
missense |
probably damaging |
1.00 |
R9003:Eps8l1
|
UTSW |
7 |
4,461,017 (GRCm38) |
missense |
possibly damaging |
0.92 |
R9023:Eps8l1
|
UTSW |
7 |
4,474,043 (GRCm38) |
nonsense |
probably null |
|
R9131:Eps8l1
|
UTSW |
7 |
4,477,574 (GRCm38) |
missense |
|
|
R9517:Eps8l1
|
UTSW |
7 |
4,477,637 (GRCm38) |
missense |
probably damaging |
1.00 |
R9653:Eps8l1
|
UTSW |
7 |
4,478,887 (GRCm38) |
missense |
unknown |
|
X0060:Eps8l1
|
UTSW |
7 |
4,470,851 (GRCm38) |
missense |
probably damaging |
0.96 |
|
Predicted Primers |
PCR Primer
(F):5'- AGTGGACCCAGTGTGTTTAG -3'
(R):5'- CACCCCTGTGATGCAGTTAG -3'
Sequencing Primer
(F):5'- ACTGGCCTTGAACTCAGAGATCTG -3'
(R):5'- AGCTGTTTTTCCACTGGG -3'
|
Posted On |
2015-10-21 |