Mutagenetix > Incidental Mutation

Incidental Mutation 'R4684:Ros1'

353528

Institutional Source

Beutler Lab

Gene Symbol

Ros1

Ensembl Gene

ENSMUSG00000019893

Gene Name

Ros1 proto-oncogene

Synonyms

Ros-1, c-ros

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.165) question?

Stock #

R4684 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

52045721-52195244 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 52129096 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 914 (N914S)

Ref Sequence

ENSEMBL: ENSMUSP00000151720 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020045] [ENSMUST00000218452] [ENSMUST00000219173] [ENSMUST00000219692]

AlphaFold

Q78DX7

Predicted Effect

probably damaging
Transcript: ENSMUST00000020045
AA Change: N935S

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000020045
Gene: ENSMUSG00000019893
AA Change: N935S

Domain	Start	End	E-Value	Type
transmembrane domain	7	26	N/A	INTRINSIC
FN3	109	187	1.05e-4	SMART
FN3	205	282	7.45e-10	SMART
LY	369	409	9.17e0	SMART
FN3	568	654	2.24e-4	SMART
LY	734	776	2.28e1	SMART
LY	777	815	4.61e0	SMART
FN3	944	1023	5.53e-4	SMART
FN3	1037	1133	1.07e1	SMART
FN3	1440	1532	1.19e1	SMART
FN3	1551	1637	2.11e0	SMART
FN3	1649	1731	6.8e-4	SMART
FN3	1746	1832	2.7e1	SMART
TyrKc	1938	2208	1.3e-145	SMART
low complexity region	2294	2307	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000117992
AA Change: N914S

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000112873
Gene: ENSMUSG00000019893
AA Change: N914S

Domain	Start	End	E-Value	Type
transmembrane domain	7	26	N/A	INTRINSIC
FN3	109	187	1.05e-4	SMART
FN3	205	282	7.45e-10	SMART
LY	369	409	9.17e0	SMART
FN3	547	633	2.24e-4	SMART
LY	713	755	2.28e1	SMART
LY	756	794	4.61e0	SMART
FN3	923	1002	5.53e-4	SMART
FN3	1016	1112	1.07e1	SMART
FN3	1419	1511	1.19e1	SMART
FN3	1530	1616	2.11e0	SMART
FN3	1628	1710	6.8e-4	SMART
FN3	1725	1811	2.7e1	SMART
TyrKc	1917	2187	1.3e-145	SMART
low complexity region	2273	2286	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000177378
AA Change: N914S

PolyPhen 2 Score 0.528 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000134905
Gene: ENSMUSG00000019893
AA Change: N914S

Domain	Start	End	E-Value	Type
transmembrane domain	7	26	N/A	INTRINSIC
FN3	109	187	1.05e-4	SMART
FN3	205	282	7.45e-10	SMART
LY	369	409	9.17e0	SMART
FN3	547	633	2.24e-4	SMART
LY	713	755	2.28e1	SMART
LY	756	794	4.61e0	SMART
FN3	923	1002	5.53e-4	SMART
FN3	1016	1112	1.07e1	SMART
Blast:LY	1190	1236	2e-18	BLAST
FN3	1419	1511	1.19e1	SMART
FN3	1530	1616	2.11e0	SMART
FN3	1628	1710	6.8e-4	SMART
FN3	1725	1811	2.7e1	SMART
transmembrane domain	1832	1854	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000218452
AA Change: N914S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000219173
AA Change: N914S

PolyPhen 2 Score 0.659 (Sensitivity: 0.86; Specificity: 0.91)

Predicted Effect

probably benign
Transcript: ENSMUST00000219692

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.0%
20x: 94.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations exhibit male infertility due to impaired sperm maturation in the epididymis. Mutant sperm are capable of fertilization in vitro but not in vivo. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310039H08Rik	T	C	17: 46,772,946 (GRCm38)	V45A	probably benign	Het
4921509C19Rik	A	G	2: 151,471,871 (GRCm38)	I629T	unknown	Het
4933402N03Rik	T	C	7: 131,138,684 (GRCm38)	R268G	probably damaging	Het
Abca13	A	T	11: 9,434,193 (GRCm38)	R3882*	probably null	Het
Adamts3	T	G	5: 89,703,007 (GRCm38)	T558P	probably damaging	Het
Ano2	A	G	6: 125,790,341 (GRCm38)	N214S	probably benign	Het
Arhgef4	A	T	1: 34,811,785 (GRCm38)		probably null	Het
Boc	C	T	16: 44,500,380 (GRCm38)	A306T	probably benign	Het
Capn10	T	C	1: 92,943,781 (GRCm38)	F367S	probably damaging	Het
Ccdc6	T	C	10: 70,189,256 (GRCm38)		probably benign	Het
Cobll1	G	T	2: 65,099,028 (GRCm38)	S688R	possibly damaging	Het
Cpxm2	G	T	7: 132,049,038 (GRCm38)	P631Q	possibly damaging	Het
Cyp2c68	A	G	19: 39,699,335 (GRCm38)	V406A	possibly damaging	Het
Cyp4a30b	T	A	4: 115,455,003 (GRCm38)	Y118N	probably damaging	Het
Dgki	A	T	6: 37,299,846 (GRCm38)		probably benign	Het
Disp2	A	G	2: 118,792,756 (GRCm38)	N1323S	probably damaging	Het
Dock1	T	A	7: 134,724,409 (GRCm38)	Y42*	probably null	Het
Eps8l1	C	A	7: 4,473,945 (GRCm38)	P471Q	probably damaging	Het
Fam20a	A	C	11: 109,721,687 (GRCm38)	L10R	unknown	Het
Fpr-rs4	T	A	17: 18,022,184 (GRCm38)	I151K	probably damaging	Het
Gga1	C	A	15: 78,885,309 (GRCm38)	P161T	probably damaging	Het
Gm8909	A	T	17: 36,165,858 (GRCm38)	H241Q	possibly damaging	Het
Gm9923	T	A	10: 72,309,476 (GRCm38)	Y52*	probably null	Het
Gucy2g	A	G	19: 55,206,256 (GRCm38)	F910L	probably damaging	Het
Helz	T	C	11: 107,649,145 (GRCm38)	V315A	probably damaging	Het
Hk2	T	C	6: 82,739,648 (GRCm38)	Y301C	probably damaging	Het
Htt	C	T	5: 34,852,765 (GRCm38)	P1521S	probably damaging	Het
Iah1	T	C	12: 21,316,433 (GRCm38)	M1T	probably null	Het
Ik	T	C	18: 36,752,414 (GRCm38)	S287P	probably damaging	Het
Itga1	T	A	13: 115,049,370 (GRCm38)	D32V	probably damaging	Het
Itpr2	A	G	6: 146,373,173 (GRCm38)	F837S	probably damaging	Het
Klk14	A	G	7: 43,691,968 (GRCm38)	I15V	probably benign	Het
Kng2	T	C	16: 22,987,641 (GRCm38)	I603V	possibly damaging	Het
Lama1	T	C	17: 67,773,778 (GRCm38)	I1267T	possibly damaging	Het
Lrp1b	A	C	2: 40,922,304 (GRCm38)	L2430V	probably benign	Het
Lrrn3	T	G	12: 41,454,244 (GRCm38)	K25Q	possibly damaging	Het
Lta4h	T	A	10: 93,468,816 (GRCm38)	N233K	probably benign	Het
Mapk13	T	C	17: 28,770,049 (GRCm38)	I53T	probably damaging	Het
Mdn1	T	C	4: 32,666,430 (GRCm38)	F123L	probably damaging	Het
Mettl7b	G	T	10: 128,960,702 (GRCm38)	C79*	probably null	Het
Myh4	G	C	11: 67,245,811 (GRCm38)	D472H	probably damaging	Het
Nipa2	A	T	7: 55,935,826 (GRCm38)	N121K	probably benign	Het
Nostrin	C	T	2: 69,183,924 (GRCm38)	T408M	probably benign	Het
Olfr347	A	G	2: 36,734,674 (GRCm38)	M118V	probably damaging	Het
Olfr725	T	C	14: 50,034,830 (GRCm38)	D191G	probably damaging	Het
Oosp2	C	T	19: 11,649,653 (GRCm38)	R102H	probably damaging	Het
Osgin2	T	A	4: 16,001,946 (GRCm38)	I202L	probably benign	Het
Pbld2	C	A	10: 63,057,697 (GRCm38)	R271S	probably damaging	Het
Pex6	C	T	17: 46,712,101 (GRCm38)	T201I	probably benign	Het
Pilra	T	C	5: 137,835,515 (GRCm38)	I96M	probably damaging	Het
Pllp	T	A	8: 94,677,278 (GRCm38)	D47V	possibly damaging	Het
Plxna2	A	G	1: 194,762,594 (GRCm38)	S765G	probably benign	Het
Prkca	A	T	11: 107,961,608 (GRCm38)	Y100N	probably damaging	Het
Prkg1	T	A	19: 31,664,179 (GRCm38)	K35*	probably null	Het
Psmc2	A	G	5: 21,803,265 (GRCm38)	D389G	possibly damaging	Het
Rnf213	A	G	11: 119,441,125 (GRCm38)	T2387A	probably damaging	Het
Ruvbl1	C	A	6: 88,491,599 (GRCm38)	T367K	probably benign	Het
Scube2	C	T	7: 109,810,713 (GRCm38)	R525H	probably damaging	Het
Sec14l4	T	C	11: 4,035,200 (GRCm38)		probably null	Het
Secisbp2l	T	C	2: 125,745,942 (GRCm38)	D751G	probably damaging	Het
Setd3	T	C	12: 108,108,690 (GRCm38)	D402G	probably benign	Het
Slc15a2	T	A	16: 36,757,849 (GRCm38)	K359N	probably damaging	Het
Slc25a21	A	G	12: 57,196,936 (GRCm38)	S2P	probably benign	Het
Slfn8	A	T	11: 83,017,506 (GRCm38)	H70Q	probably benign	Het
Spef2	T	C	15: 9,647,490 (GRCm38)	I944V	probably benign	Het
Spg11	A	G	2: 122,065,076 (GRCm38)	F1887S	probably damaging	Het
Sptbn4	T	C	7: 27,364,419 (GRCm38)	E879G	probably damaging	Het
Sptbn4	A	T	7: 27,366,735 (GRCm38)	D649E	possibly damaging	Het
Stx5a	C	A	19: 8,743,361 (GRCm38)	R121S	probably damaging	Het
Tbcd	T	C	11: 121,493,771 (GRCm38)	L26P	probably damaging	Het
Tecpr1	T	C	5: 144,207,437 (GRCm38)	D649G	probably benign	Het
Tfam	A	G	10: 71,237,847 (GRCm38)	S32P	probably benign	Het
Trmt44	C	T	5: 35,558,043 (GRCm38)	R642H	probably benign	Het
Trpm3	G	A	19: 22,987,781 (GRCm38)	A1547T	probably benign	Het
Ttll6	T	C	11: 96,153,177 (GRCm38)	V519A	probably benign	Het
Umodl1	T	C	17: 30,998,114 (GRCm38)	F1107L	probably benign	Het
Usp5	A	T	6: 124,817,956 (GRCm38)	V677E	probably damaging	Het
Utp20	A	T	10: 88,807,445 (GRCm38)	L605*	probably null	Het
Utrn	T	C	10: 12,745,240 (GRCm38)	D229G	probably damaging	Het
Uty	A	T	Y: 1,176,502 (GRCm38)	L178*	probably null	Het
Vmn2r88	A	T	14: 51,413,334 (GRCm38)	D168V	possibly damaging	Het
Vps13b	T	C	15: 35,646,178 (GRCm38)	V1476A	probably damaging	Het
Vps13b	C	T	15: 35,841,341 (GRCm38)	H2506Y	probably benign	Het
Vps13b	C	A	15: 35,879,821 (GRCm38)	T3014K	probably benign	Het
Vps37c	T	C	19: 10,712,768 (GRCm38)	V198A	probably benign	Het
Zfc3h1	T	C	10: 115,423,385 (GRCm38)	Y1621H	probably benign	Het
Zfp251	T	C	15: 76,854,407 (GRCm38)	D162G	possibly damaging	Het
Zfp292	T	C	4: 34,807,078 (GRCm38)	T1994A	probably benign	Het
Zfp791	A	G	8: 85,110,930 (GRCm38)	Y102H	probably benign	Het

Other mutations in Ros1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00236:Ros1	APN	10	52,194,890 (GRCm38)	missense	probably benign	0.01
IGL00338:Ros1	APN	10	52,125,811 (GRCm38)	missense	probably benign
IGL00419:Ros1	APN	10	52,091,054 (GRCm38)	missense	probably damaging	0.97
IGL00840:Ros1	APN	10	52,144,873 (GRCm38)	missense	possibly damaging	0.92
IGL00841:Ros1	APN	10	52,144,873 (GRCm38)	missense	possibly damaging	0.92
IGL00951:Ros1	APN	10	52,143,252 (GRCm38)	missense	probably damaging	0.99
IGL01123:Ros1	APN	10	52,120,809 (GRCm38)	missense	probably damaging	1.00
IGL01128:Ros1	APN	10	52,142,328 (GRCm38)	nonsense	probably null
IGL01300:Ros1	APN	10	52,101,713 (GRCm38)	missense	probably benign	0.01
IGL01316:Ros1	APN	10	52,087,879 (GRCm38)	critical splice donor site	probably null
IGL01349:Ros1	APN	10	52,051,026 (GRCm38)	missense	probably damaging	0.99
IGL01363:Ros1	APN	10	52,166,142 (GRCm38)	missense	probably damaging	1.00
IGL01457:Ros1	APN	10	52,046,330 (GRCm38)	splice site	probably benign
IGL01532:Ros1	APN	10	52,090,938 (GRCm38)	splice site	probably benign
IGL01585:Ros1	APN	10	52,155,102 (GRCm38)	missense	probably damaging	1.00
IGL01650:Ros1	APN	10	52,154,979 (GRCm38)	missense	probably damaging	0.99
IGL01672:Ros1	APN	10	52,101,803 (GRCm38)	missense	possibly damaging	0.92
IGL01904:Ros1	APN	10	52,077,911 (GRCm38)	missense	probably damaging	0.97
IGL02040:Ros1	APN	10	52,115,922 (GRCm38)	missense	probably damaging	0.99
IGL02053:Ros1	APN	10	52,162,720 (GRCm38)	missense	probably damaging	1.00
IGL02147:Ros1	APN	10	52,120,895 (GRCm38)	missense	probably damaging	1.00
IGL02169:Ros1	APN	10	52,081,957 (GRCm38)	critical splice donor site	probably null
IGL02247:Ros1	APN	10	52,129,581 (GRCm38)	missense	probably damaging	0.99
IGL02262:Ros1	APN	10	52,178,969 (GRCm38)	missense	probably damaging	0.96
IGL02307:Ros1	APN	10	52,128,438 (GRCm38)	missense	possibly damaging	0.53
IGL02398:Ros1	APN	10	52,144,884 (GRCm38)	splice site	probably benign
IGL02525:Ros1	APN	10	52,116,042 (GRCm38)	missense	possibly damaging	0.66
IGL02718:Ros1	APN	10	52,118,232 (GRCm38)	missense	probably damaging	1.00
IGL02721:Ros1	APN	10	52,172,831 (GRCm38)	splice site	probably benign
IGL02808:Ros1	APN	10	52,125,889 (GRCm38)	missense	probably damaging	1.00
IGL03009:Ros1	APN	10	52,145,907 (GRCm38)	missense	probably benign	0.00
IGL03035:Ros1	APN	10	52,075,984 (GRCm38)	splice site	probably benign
IGL03092:Ros1	APN	10	52,098,806 (GRCm38)	missense	probably damaging	0.99
IGL03309:Ros1	APN	10	52,118,261 (GRCm38)	missense	possibly damaging	0.83
IGL03333:Ros1	APN	10	52,155,171 (GRCm38)	missense	probably damaging	1.00
boss	UTSW	10	52,090,995 (GRCm38)	nonsense	probably null
Chuckwagon	UTSW	10	52,118,203 (GRCm38)	missense	probably damaging	1.00
R1005_Ros1_648	UTSW	10	52,128,405 (GRCm38)	splice site	probably benign
R1220_Ros1_012	UTSW	10	52,098,870 (GRCm38)	missense	probably damaging	0.97
R3423_Ros1_122	UTSW	10	52,128,416 (GRCm38)	splice site	probably null
trail	UTSW	10	52,161,895 (GRCm38)	nonsense	probably null
R0049:Ros1	UTSW	10	52,101,761 (GRCm38)	missense	possibly damaging	0.66
R0049:Ros1	UTSW	10	52,101,761 (GRCm38)	missense	possibly damaging	0.66
R0050:Ros1	UTSW	10	52,101,803 (GRCm38)	missense	probably damaging	0.97
R0050:Ros1	UTSW	10	52,101,803 (GRCm38)	missense	probably damaging	0.97
R0057:Ros1	UTSW	10	52,180,191 (GRCm38)	missense	probably benign	0.00
R0057:Ros1	UTSW	10	52,180,191 (GRCm38)	missense	probably benign	0.00
R0106:Ros1	UTSW	10	52,142,267 (GRCm38)	missense	possibly damaging	0.85
R0106:Ros1	UTSW	10	52,142,267 (GRCm38)	missense	possibly damaging	0.85
R0125:Ros1	UTSW	10	52,125,789 (GRCm38)	missense	probably benign	0.38
R0403:Ros1	UTSW	10	52,143,438 (GRCm38)	splice site	probably benign
R0487:Ros1	UTSW	10	52,155,108 (GRCm38)	missense	possibly damaging	0.69
R0502:Ros1	UTSW	10	52,194,823 (GRCm38)	splice site	probably benign
R0557:Ros1	UTSW	10	52,085,263 (GRCm38)	missense	possibly damaging	0.82
R0599:Ros1	UTSW	10	52,123,300 (GRCm38)	missense	probably damaging	1.00
R0620:Ros1	UTSW	10	52,118,348 (GRCm38)	missense	probably damaging	1.00
R0679:Ros1	UTSW	10	52,066,295 (GRCm38)	missense	possibly damaging	0.95
R1005:Ros1	UTSW	10	52,128,405 (GRCm38)	splice site	probably benign
R1073:Ros1	UTSW	10	52,046,125 (GRCm38)	missense	probably damaging	1.00
R1220:Ros1	UTSW	10	52,098,870 (GRCm38)	missense	probably damaging	0.97
R1279:Ros1	UTSW	10	52,142,166 (GRCm38)	missense	possibly damaging	0.81
R1295:Ros1	UTSW	10	52,087,932 (GRCm38)	missense	possibly damaging	0.92
R1336:Ros1	UTSW	10	52,168,662 (GRCm38)	missense	probably damaging	1.00
R1371:Ros1	UTSW	10	52,087,945 (GRCm38)	missense	probably damaging	0.98
R1447:Ros1	UTSW	10	52,098,858 (GRCm38)	missense	possibly damaging	0.66
R1486:Ros1	UTSW	10	52,172,858 (GRCm38)	missense	probably damaging	1.00
R1499:Ros1	UTSW	10	52,098,677 (GRCm38)	missense	possibly damaging	0.92
R1669:Ros1	UTSW	10	52,161,811 (GRCm38)	missense	probably damaging	1.00
R1744:Ros1	UTSW	10	52,123,379 (GRCm38)	missense	probably damaging	0.99
R1759:Ros1	UTSW	10	52,120,826 (GRCm38)	missense	probably damaging	1.00
R1791:Ros1	UTSW	10	52,100,087 (GRCm38)	missense	probably benign	0.00
R1794:Ros1	UTSW	10	52,124,103 (GRCm38)	nonsense	probably null
R2031:Ros1	UTSW	10	52,067,068 (GRCm38)	missense	possibly damaging	0.88
R2115:Ros1	UTSW	10	52,128,555 (GRCm38)	missense	probably benign	0.00
R2219:Ros1	UTSW	10	52,166,079 (GRCm38)	missense	probably damaging	1.00
R2290:Ros1	UTSW	10	52,118,381 (GRCm38)	missense	probably damaging	0.96
R2329:Ros1	UTSW	10	52,162,887 (GRCm38)	missense	probably damaging	1.00
R2371:Ros1	UTSW	10	52,163,895 (GRCm38)	missense	possibly damaging	0.66
R2879:Ros1	UTSW	10	52,172,840 (GRCm38)	critical splice donor site	probably null
R3154:Ros1	UTSW	10	52,050,981 (GRCm38)	missense	probably benign
R3423:Ros1	UTSW	10	52,128,416 (GRCm38)	splice site	probably null
R3424:Ros1	UTSW	10	52,128,416 (GRCm38)	splice site	probably null
R3425:Ros1	UTSW	10	52,128,416 (GRCm38)	splice site	probably null
R3433:Ros1	UTSW	10	52,091,108 (GRCm38)	missense	probably benign	0.45
R3522:Ros1	UTSW	10	52,090,995 (GRCm38)	nonsense	probably null
R3686:Ros1	UTSW	10	52,145,816 (GRCm38)	missense	probably damaging	1.00
R3710:Ros1	UTSW	10	52,161,895 (GRCm38)	nonsense	probably null
R3771:Ros1	UTSW	10	52,128,991 (GRCm38)	missense	probably damaging	0.97
R3808:Ros1	UTSW	10	52,120,848 (GRCm38)	missense	probably benign	0.08
R3930:Ros1	UTSW	10	52,194,848 (GRCm38)	missense	possibly damaging	0.92
R3950:Ros1	UTSW	10	52,066,388 (GRCm38)	missense	probably damaging	1.00
R3981:Ros1	UTSW	10	52,120,878 (GRCm38)	missense	possibly damaging	0.46
R4007:Ros1	UTSW	10	52,118,232 (GRCm38)	missense	probably damaging	1.00
R4346:Ros1	UTSW	10	52,168,609 (GRCm38)	missense	possibly damaging	0.92
R4382:Ros1	UTSW	10	52,120,959 (GRCm38)	missense	possibly damaging	0.46
R4414:Ros1	UTSW	10	52,162,704 (GRCm38)	critical splice donor site	probably null
R4450:Ros1	UTSW	10	52,077,942 (GRCm38)	missense	probably damaging	0.98
R4468:Ros1	UTSW	10	52,118,356 (GRCm38)	missense	probably damaging	1.00
R4569:Ros1	UTSW	10	52,163,994 (GRCm38)	missense	probably damaging	0.99
R4649:Ros1	UTSW	10	52,129,668 (GRCm38)	missense	possibly damaging	0.66
R4706:Ros1	UTSW	10	52,101,894 (GRCm38)	missense	possibly damaging	0.95
R4731:Ros1	UTSW	10	52,142,229 (GRCm38)	missense	probably damaging	1.00
R4748:Ros1	UTSW	10	52,115,997 (GRCm38)	missense	probably benign	0.00
R4806:Ros1	UTSW	10	52,096,175 (GRCm38)	missense	probably damaging	0.96
R4865:Ros1	UTSW	10	52,172,870 (GRCm38)	missense	probably damaging	0.99
R4973:Ros1	UTSW	10	52,154,991 (GRCm38)	missense	probably damaging	0.98
R5022:Ros1	UTSW	10	52,124,075 (GRCm38)	missense	possibly damaging	0.46
R5033:Ros1	UTSW	10	52,128,416 (GRCm38)	critical splice donor site	probably null
R5082:Ros1	UTSW	10	52,163,941 (GRCm38)	missense	possibly damaging	0.66
R5083:Ros1	UTSW	10	52,163,941 (GRCm38)	missense	possibly damaging	0.66
R5130:Ros1	UTSW	10	52,163,941 (GRCm38)	missense	possibly damaging	0.66
R5269:Ros1	UTSW	10	52,051,008 (GRCm38)	missense	probably damaging	1.00
R5399:Ros1	UTSW	10	52,090,944 (GRCm38)	critical splice donor site	probably null
R5414:Ros1	UTSW	10	52,155,093 (GRCm38)	missense	probably damaging	1.00
R5659:Ros1	UTSW	10	52,143,386 (GRCm38)	missense	possibly damaging	0.92
R5742:Ros1	UTSW	10	52,142,138 (GRCm38)	critical splice donor site	probably null
R5780:Ros1	UTSW	10	52,194,857 (GRCm38)	missense	probably damaging	1.00
R5805:Ros1	UTSW	10	52,123,289 (GRCm38)	missense	probably damaging	1.00
R5843:Ros1	UTSW	10	52,166,197 (GRCm38)	missense	possibly damaging	0.92
R5881:Ros1	UTSW	10	52,181,798 (GRCm38)	missense	probably benign	0.26
R6027:Ros1	UTSW	10	52,163,968 (GRCm38)	missense	possibly damaging	0.82
R6035:Ros1	UTSW	10	52,077,971 (GRCm38)	missense	probably benign
R6035:Ros1	UTSW	10	52,077,971 (GRCm38)	missense	probably benign
R6052:Ros1	UTSW	10	52,163,903 (GRCm38)	missense	probably benign	0.39
R6175:Ros1	UTSW	10	52,101,785 (GRCm38)	missense	probably benign	0.02
R6315:Ros1	UTSW	10	52,118,210 (GRCm38)	missense	probably benign
R6342:Ros1	UTSW	10	52,155,255 (GRCm38)	missense	probably damaging	1.00
R6470:Ros1	UTSW	10	52,166,044 (GRCm38)	critical splice donor site	probably null
R6527:Ros1	UTSW	10	52,143,377 (GRCm38)	missense	possibly damaging	0.66
R6568:Ros1	UTSW	10	52,162,812 (GRCm38)	missense	probably damaging	1.00
R6573:Ros1	UTSW	10	52,155,010 (GRCm38)	missense	possibly damaging	0.84
R6653:Ros1	UTSW	10	52,142,203 (GRCm38)	missense	probably damaging	1.00
R6959:Ros1	UTSW	10	52,163,994 (GRCm38)	missense	probably damaging	0.99
R7011:Ros1	UTSW	10	52,180,176 (GRCm38)	missense	probably damaging	1.00
R7111:Ros1	UTSW	10	52,181,810 (GRCm38)	missense	probably benign	0.02
R7243:Ros1	UTSW	10	52,123,381 (GRCm38)	missense	probably damaging	1.00
R7355:Ros1	UTSW	10	52,166,079 (GRCm38)	missense	probably damaging	1.00
R7385:Ros1	UTSW	10	52,155,126 (GRCm38)	missense	probably benign	0.00
R7460:Ros1	UTSW	10	52,118,203 (GRCm38)	missense	probably damaging	1.00
R7549:Ros1	UTSW	10	52,145,834 (GRCm38)	missense	probably damaging	0.96
R7573:Ros1	UTSW	10	52,169,976 (GRCm38)	missense	probably benign	0.03
R7650:Ros1	UTSW	10	52,046,209 (GRCm38)	missense	probably benign	0.00
R7667:Ros1	UTSW	10	52,163,971 (GRCm38)	missense	probably damaging	1.00
R7696:Ros1	UTSW	10	52,142,283 (GRCm38)	missense	probably damaging	1.00
R7785:Ros1	UTSW	10	52,162,848 (GRCm38)	missense	probably damaging	1.00
R7814:Ros1	UTSW	10	52,096,137 (GRCm38)	missense	probably benign	0.28
R7830:Ros1	UTSW	10	52,154,934 (GRCm38)	missense	probably damaging	0.99
R7832:Ros1	UTSW	10	52,144,861 (GRCm38)	missense	probably damaging	0.99
R7854:Ros1	UTSW	10	52,128,467 (GRCm38)	missense	probably damaging	1.00
R7912:Ros1	UTSW	10	52,168,695 (GRCm38)	missense	probably damaging	1.00
R7972:Ros1	UTSW	10	52,154,830 (GRCm38)	nonsense	probably null
R7993:Ros1	UTSW	10	52,123,347 (GRCm38)	missense	probably benign	0.34
R8036:Ros1	UTSW	10	52,165,343 (GRCm38)	missense	probably benign
R8137:Ros1	UTSW	10	52,125,837 (GRCm38)	missense	possibly damaging	0.87
R8169:Ros1	UTSW	10	52,064,672 (GRCm38)	critical splice donor site	probably null
R8199:Ros1	UTSW	10	52,101,717 (GRCm38)	nonsense	probably null
R8293:Ros1	UTSW	10	52,087,918 (GRCm38)	missense	probably damaging	1.00
R8368:Ros1	UTSW	10	52,064,737 (GRCm38)	missense	probably damaging	1.00
R8406:Ros1	UTSW	10	52,101,845 (GRCm38)	missense	possibly damaging	0.56
R8471:Ros1	UTSW	10	52,120,982 (GRCm38)	missense	probably benign	0.00
R8498:Ros1	UTSW	10	52,178,951 (GRCm38)	missense	probably damaging	0.99
R8532:Ros1	UTSW	10	52,098,756 (GRCm38)	missense	possibly damaging	0.92
R8678:Ros1	UTSW	10	52,087,902 (GRCm38)	missense	probably benign
R8726:Ros1	UTSW	10	52,078,673 (GRCm38)	missense	possibly damaging	0.46
R8789:Ros1	UTSW	10	52,123,232 (GRCm38)	missense	probably damaging	0.99
R8799:Ros1	UTSW	10	52,046,047 (GRCm38)	missense	probably benign	0.08
R8915:Ros1	UTSW	10	52,101,709 (GRCm38)	splice site	probably benign
R8958:Ros1	UTSW	10	52,096,094 (GRCm38)	missense	probably damaging	1.00
R8972:Ros1	UTSW	10	52,123,237 (GRCm38)	missense	probably benign	0.05
R9020:Ros1	UTSW	10	52,154,927 (GRCm38)	missense	probably benign	0.32
R9147:Ros1	UTSW	10	52,050,943 (GRCm38)	missense	probably benign
R9154:Ros1	UTSW	10	52,046,205 (GRCm38)	missense	possibly damaging	0.87
R9189:Ros1	UTSW	10	52,143,406 (GRCm38)	missense	probably damaging	0.99
R9341:Ros1	UTSW	10	52,096,020 (GRCm38)	critical splice donor site	probably null
R9343:Ros1	UTSW	10	52,096,020 (GRCm38)	critical splice donor site	probably null
R9407:Ros1	UTSW	10	52,118,395 (GRCm38)	missense	probably damaging	1.00
R9428:Ros1	UTSW	10	52,081,965 (GRCm38)	missense	probably benign	0.00
R9502:Ros1	UTSW	10	52,124,078 (GRCm38)	missense	probably benign	0.00
R9531:Ros1	UTSW	10	52,130,967 (GRCm38)	missense	probably damaging	1.00
R9546:Ros1	UTSW	10	52,118,119 (GRCm38)	critical splice donor site	probably null
R9562:Ros1	UTSW	10	52,067,074 (GRCm38)	missense	probably damaging	1.00
R9565:Ros1	UTSW	10	52,067,074 (GRCm38)	missense	probably damaging	1.00
R9604:Ros1	UTSW	10	52,118,153 (GRCm38)	missense	probably damaging	1.00
R9645:Ros1	UTSW	10	52,072,052 (GRCm38)	critical splice donor site	probably null
R9658:Ros1	UTSW	10	52,090,973 (GRCm38)	missense	probably damaging	0.99
R9664:Ros1	UTSW	10	52,120,835 (GRCm38)	missense	probably benign	0.18
RF018:Ros1	UTSW	10	52,155,121 (GRCm38)	missense	probably benign
Z1176:Ros1	UTSW	10	52,091,109 (GRCm38)	missense	possibly damaging	0.89
Z1177:Ros1	UTSW	10	52,168,671 (GRCm38)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- ACCAGCAACAGACCTTGGAATG -3'
(R):5'- GACATCTCTCAAGCCTCTGC -3'

Sequencing Primer
(F):5'- GACCTTGGAATGAGTGCTAAATTCC -3'
(R):5'- TCTTGAGTAGCCAAGATTGACG -3'

Posted On

2015-10-21