Incidental Mutation 'R4688:Lrrc74a'
ID353889
Institutional Source Beutler Lab
Gene Symbol Lrrc74a
Ensembl Gene ENSMUSG00000059114
Gene Nameleucine rich repeat containing 74A
SynonymsLrrc74, Gm6772
MMRRC Submission 041939-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.060) question?
Stock #R4688 (G1)
Quality Score225
Status Not validated
Chromosome12
Chromosomal Location86734369-86763797 bp(+) (GRCm38)
Type of Mutationnonsense
DNA Base Change (assembly) C to T at 86737698 bp
ZygosityHeterozygous
Amino Acid Change Glutamine to Stop codon at position 67 (Q67*)
Ref Sequence ENSEMBL: ENSMUSP00000152661 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000095527] [ENSMUST00000222180] [ENSMUST00000223308]
Predicted Effect probably null
Transcript: ENSMUST00000095527
AA Change: Q67*
SMART Domains Protein: ENSMUSP00000093183
Gene: ENSMUSG00000059114
AA Change: Q67*

DomainStartEndE-ValueType
Blast:LRR 87 116 9e-7 BLAST
LRR 117 144 4.17e-3 SMART
LRR 145 172 3.16e-3 SMART
LRR 174 201 1.92e-2 SMART
LRR 202 229 3.07e-1 SMART
LRR 230 257 1.03e-2 SMART
LRR 258 285 1.64e-1 SMART
LRR 286 313 2.03e0 SMART
LRR 314 341 1.11e1 SMART
Predicted Effect probably null
Transcript: ENSMUST00000222180
AA Change: Q67*
Predicted Effect probably benign
Transcript: ENSMUST00000223197
Predicted Effect probably null
Transcript: ENSMUST00000223308
AA Change: Q67*
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 94.3%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 96 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
0610010F05Rik A G 11: 23,593,449 S530P probably benign Het
1700003E16Rik A G 6: 83,162,698 N535S probably damaging Het
2810403A07Rik T A 3: 88,686,517 M71K probably damaging Het
9930021J03Rik A G 19: 29,717,101 I1664T probably benign Het
Abcc12 T C 8: 86,548,694 S452G possibly damaging Het
Acacb C A 5: 114,204,763 Q897K probably benign Het
Acot3 C A 12: 84,053,917 R145S probably damaging Het
AI314180 A G 4: 58,840,757 V667A probably damaging Het
Ankrd54 A T 15: 79,054,582 Y247N probably damaging Het
Arl11 G A 14: 61,311,097 V119I probably benign Het
Atxn7 T A 14: 14,089,288 M268K probably benign Het
Bms1 G A 6: 118,392,706 R934C probably damaging Het
Ccdc129 G A 6: 55,967,147 probably null Het
Chrnb3 T C 8: 27,394,119 S295P probably damaging Het
Cic TCCCCC TCCCCCCC 7: 25,291,670 probably null Het
Cnr1 A T 4: 33,944,571 I320F probably benign Het
Cntn4 C T 6: 106,437,949 P147L probably damaging Het
Col24a1 G A 3: 145,314,383 V172I probably benign Het
Col9a3 A G 2: 180,607,631 D262G probably damaging Het
Csrnp2 A G 15: 100,482,360 V350A probably damaging Het
D630045J12Rik A G 6: 38,196,657 V192A possibly damaging Het
Deptor A G 15: 55,208,781 M219V probably benign Het
Dmrtb1 A T 4: 107,684,050 L38Q probably damaging Het
Dvl2 G A 11: 70,007,518 R367Q possibly damaging Het
Dync1h1 T G 12: 110,655,528 I3435S probably damaging Het
Eif2b3 A G 4: 117,058,849 N218D probably benign Het
Epha2 A G 4: 141,318,981 D497G probably benign Het
Epha7 G T 4: 28,821,367 L177F probably damaging Het
Fam214b A G 4: 43,034,663 F352S probably damaging Het
Fam98c C T 7: 29,155,241 E147K probably damaging Het
Fbxo17 A G 7: 28,732,554 T19A probably benign Het
Fbxo47 A G 11: 97,856,223 F339S probably damaging Het
Frmd4a G T 2: 4,537,311 V234L possibly damaging Het
Gal3st2 A G 1: 93,872,523 D32G probably damaging Het
Gpr135 T C 12: 72,070,946 T16A probably benign Het
Gpr160 A T 3: 30,896,686 R302S probably benign Het
Hrh2 C A 13: 54,214,801 N265K probably benign Het
Htatip2 C A 7: 49,773,423 A242E probably damaging Het
Igfbp7 T C 5: 77,407,635 Y127C probably damaging Het
Igkv16-104 A G 6: 68,425,894 Q57R possibly damaging Het
Ino80c A G 18: 24,108,846 S161P probably damaging Het
Kcnc1 A G 7: 46,397,835 D53G probably benign Het
Lce1h G T 3: 92,763,567 R93S unknown Het
Lce1k T C 3: 92,806,644 S78G unknown Het
Lhcgr T A 17: 88,765,152 I156F probably damaging Het
Lpl T C 8: 68,899,425 Y343H probably damaging Het
Lrp6 G T 6: 134,479,743 R853S probably damaging Het
Lrrc7 A G 3: 158,148,605 V1322A probably damaging Het
Megf6 A T 4: 154,253,814 D447V probably damaging Het
Mep1a T C 17: 43,482,248 D355G possibly damaging Het
Ncoa1 T C 12: 4,315,781 D95G probably benign Het
Npepl1 A T 2: 174,114,442 I139F possibly damaging Het
Nrcam T C 12: 44,547,237 S262P probably benign Het
Nrp1 A G 8: 128,502,566 N842D probably benign Het
Olfml3 A G 3: 103,732,181 probably benign Het
Olfr1375 A G 11: 51,048,988 R294G probably damaging Het
Olfr1505 A T 19: 13,919,241 T74S probably benign Het
Olfr32 T A 2: 90,138,999 N47Y possibly damaging Het
Olfr421-ps1 T C 1: 174,151,596 Y27H possibly damaging Het
Olfr711 T A 7: 106,971,861 Y161F probably benign Het
Olfr773 G A 10: 129,186,645 P259S probably damaging Het
Olfr918 A G 9: 38,673,363 L27P probably damaging Het
Pde2a A G 7: 101,502,834 N316S probably benign Het
Pde4dip T A 3: 97,843,677 R74* probably null Het
Pex13 A T 11: 23,655,472 W253R possibly damaging Het
Piezo1 A T 8: 122,488,539 W1444R probably damaging Het
Pla2g4e T C 2: 120,167,933 K843R possibly damaging Het
Plxna2 C T 1: 194,644,445 P229L probably damaging Het
Prelid3b G T 2: 174,466,799 T131K probably benign Het
Pros1 T C 16: 62,889,007 probably null Het
Prrc2c G T 1: 162,697,687 P450Q unknown Het
Ptbp1 G T 10: 79,856,508 V5F possibly damaging Het
Ptk2 T A 15: 73,206,225 L997F probably damaging Het
Rims1 G T 1: 22,479,447 S525* probably null Het
Sh2b3 T A 5: 121,818,634 D318V probably benign Het
Slc16a13 A T 11: 70,220,275 I88N probably damaging Het
Slit2 T A 5: 48,257,003 probably null Het
Snx10 A G 6: 51,579,938 N67S probably damaging Het
Stil A G 4: 115,041,308 Y1045C probably damaging Het
Stra6 A G 9: 58,135,076 probably null Het
Sympk A G 7: 19,054,410 S1254G probably benign Het
Syt15 G T 14: 34,228,054 G377V probably damaging Het
Taar4 A T 10: 23,960,833 I114F probably damaging Het
Tcaf3 G A 6: 42,593,366 probably null Het
Tgm7 A T 2: 121,094,021 N558K probably benign Het
Tln2 T G 9: 67,397,653 M1L probably benign Het
Trim50 C T 5: 135,367,140 T314I probably damaging Het
Trp53rka A T 2: 165,491,392 Y192* probably null Het
Ube3b T C 5: 114,393,078 V211A probably benign Het
Ush2a A G 1: 188,399,941 S787G probably benign Het
Vmn1r189 T C 13: 22,102,119 M183V probably damaging Het
Vps13d G T 4: 145,178,212 Q115K probably benign Het
Zfp358 A G 8: 3,495,493 D25G probably damaging Het
Zfp521 T G 18: 13,844,590 K922T probably damaging Het
Zfp521 T A 18: 13,844,591 K922* probably null Het
Zfp68 T A 5: 138,616,481 K4* probably null Het
Other mutations in Lrrc74a
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01370:Lrrc74a APN 12 86754430 missense probably damaging 1.00
IGL01380:Lrrc74a APN 12 86761722 missense possibly damaging 0.62
IGL01715:Lrrc74a APN 12 86754415 missense probably benign 0.05
IGL01832:Lrrc74a APN 12 86761714 missense probably benign 0.00
IGL01953:Lrrc74a APN 12 86741720 missense probably damaging 1.00
IGL02218:Lrrc74a APN 12 86749048 missense probably benign 0.15
IGL02637:Lrrc74a APN 12 86741747 nonsense probably null
IGL03397:Lrrc74a APN 12 86758538 missense probably benign 0.39
R0201:Lrrc74a UTSW 12 86761773 splice site probably benign
R0360:Lrrc74a UTSW 12 86737795 missense probably damaging 1.00
R0403:Lrrc74a UTSW 12 86740979 missense probably damaging 1.00
R0729:Lrrc74a UTSW 12 86745579 nonsense probably null
R1675:Lrrc74a UTSW 12 86741026 missense probably damaging 1.00
R1774:Lrrc74a UTSW 12 86749053 missense probably damaging 1.00
R1818:Lrrc74a UTSW 12 86737710 missense probably damaging 1.00
R6023:Lrrc74a UTSW 12 86758606 missense probably damaging 1.00
R6190:Lrrc74a UTSW 12 86736489 missense probably benign 0.01
R6226:Lrrc74a UTSW 12 86748457 missense possibly damaging 0.87
R6247:Lrrc74a UTSW 12 86758556 missense probably damaging 1.00
R7275:Lrrc74a UTSW 12 86740979 missense probably damaging 1.00
R7631:Lrrc74a UTSW 12 86749110 missense probably damaging 1.00
R7857:Lrrc74a UTSW 12 86741711 missense probably benign 0.00
R8172:Lrrc74a UTSW 12 86741756 missense probably damaging 1.00
X0024:Lrrc74a UTSW 12 86749045 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ACACATGGAAAAGCTGGGTTC -3'
(R):5'- GTTCCCTCTGAATCTCAAACCAGC -3'

Sequencing Primer
(F):5'- GCTGGGTTCTAGAGCACATAC -3'
(R):5'- AATCCTCTTCTGTCAGGTTCGATGTG -3'
Posted On2015-10-21