Incidental Mutation 'R4705:Pla2g15'
ID 355129
Institutional Source Beutler Lab
Gene Symbol Pla2g15
Ensembl Gene ENSMUSG00000031903
Gene Name phospholipase A2, group XV
Synonyms Lpla2, LLPL, Lypla3, C87498
MMRRC Submission 041953-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.155) question?
Stock # R4705 (G1)
Quality Score 225
Status Validated
Chromosome 8
Chromosomal Location 106150399-106164715 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to A at 106163059 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Methionine to Lysine at position 321 (M321K)
Ref Sequence ENSEMBL: ENSMUSP00000034377 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000034377] [ENSMUST00000212963]
AlphaFold Q8VEB4
Predicted Effect probably benign
Transcript: ENSMUST00000034377
AA Change: M321K

PolyPhen 2 Score 0.084 (Sensitivity: 0.93; Specificity: 0.85)
SMART Domains Protein: ENSMUSP00000034377
Gene: ENSMUSG00000031903
AA Change: M321K

signal peptide 1 33 N/A INTRINSIC
Pfam:LCAT 72 399 6.2e-84 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000212059
Predicted Effect noncoding transcript
Transcript: ENSMUST00000212116
Predicted Effect probably benign
Transcript: ENSMUST00000212963
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 96.8%
  • 20x: 94.2%
Validation Efficiency 96% (113/118)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice do not develop atherosclerotic lesions even when fed an atherogenic diet. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 102 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1190002N15Rik T C 9: 94,520,635 (GRCm38) N325D possibly damaging Het
1700056E22Rik A G 1: 184,033,172 (GRCm38) V230A possibly damaging Het
2010315B03Rik T C 9: 124,294,001 (GRCm38) T123A possibly damaging Het
4932438A13Rik C T 3: 37,041,889 (GRCm38) T1108I probably benign Het
Abca4 T C 3: 122,105,370 (GRCm38) V667A probably damaging Het
Abcb5 A G 12: 118,965,305 (GRCm38) S4P possibly damaging Het
Adam25 G T 8: 40,754,126 (GRCm38) C143F probably damaging Het
Ahnak C A 19: 9,016,906 (GRCm38) H5185N probably benign Het
Apobec4 A G 1: 152,756,250 (GRCm38) T10A probably benign Het
Ascc1 A G 10: 60,049,802 (GRCm38) Y225C probably damaging Het
Aspscr1 A T 11: 120,688,945 (GRCm38) K39N possibly damaging Het
Atf7ip C T 6: 136,561,194 (GRCm38) P483L probably damaging Het
Atp11a C G 8: 12,813,118 (GRCm38) P99R probably damaging Het
B4galnt1 G T 10: 127,167,525 (GRCm38) V172F possibly damaging Het
Bag6 C A 17: 35,142,343 (GRCm38) P476H probably damaging Het
BC049730 T A 7: 24,713,509 (GRCm38) L114Q probably damaging Het
C2cd3 A G 7: 100,395,188 (GRCm38) K326E possibly damaging Het
Casp1 A G 9: 5,306,204 (GRCm38) D363G probably damaging Het
Ccdc33 G T 9: 58,117,557 (GRCm38) Q129K probably benign Het
Ccdc88a T C 11: 29,422,586 (GRCm38) I107T probably benign Het
Cela2a T C 4: 141,821,411 (GRCm38) N138S probably benign Het
Cfap61 A C 2: 146,035,202 (GRCm38) R460S probably damaging Het
Clstn2 T C 9: 97,463,559 (GRCm38) N579D possibly damaging Het
Col13a1 C A 10: 61,850,165 (GRCm38) G683W unknown Het
Col4a2 G A 8: 11,313,504 (GRCm38) R14Q possibly damaging Het
Cpa6 A G 1: 10,481,058 (GRCm38) S164P probably benign Het
Cpq A G 15: 33,497,338 (GRCm38) N408S probably benign Het
Ctnnal1 T C 4: 56,812,579 (GRCm38) T690A probably benign Het
Cx3cl1 A T 8: 94,780,207 (GRCm38) N280I probably benign Het
Cyp2b19 C T 7: 26,757,292 (GRCm38) R36C probably benign Het
Ddx51 T C 5: 110,655,308 (GRCm38) V269A probably damaging Het
Dlst G T 12: 85,118,842 (GRCm38) probably null Het
Dmkn G C 7: 30,763,981 (GRCm38) A20P probably damaging Het
Dnhd1 T C 7: 105,655,741 (GRCm38) I330T probably damaging Het
Dock3 G A 9: 107,025,336 (GRCm38) H292Y probably damaging Het
Ell A G 8: 70,578,934 (GRCm38) D94G possibly damaging Het
Enam T A 5: 88,503,791 (GRCm38) L1053* probably null Het
Fcgbp A G 7: 28,107,296 (GRCm38) K2230E probably benign Het
Frmd5 G T 2: 121,562,863 (GRCm38) probably benign Het
Gas2l1 G A 11: 5,060,867 (GRCm38) S654L possibly damaging Het
Gltpd2 G T 11: 70,520,140 (GRCm38) E86* probably null Het
Glyat T C 19: 12,651,297 (GRCm38) L152P possibly damaging Het
Gm17330 T C 12: 23,968,782 (GRCm38) T22A probably damaging Het
Gm9931 T A 1: 147,281,853 (GRCm38) noncoding transcript Het
Gpatch1 A T 7: 35,299,305 (GRCm38) probably null Het
Gpr4 T C 7: 19,222,894 (GRCm38) L247P probably damaging Het
Gtpbp3 G A 8: 71,491,114 (GRCm38) E214K probably benign Het
Hdac7 G T 15: 97,811,587 (GRCm38) Q21K probably damaging Het
Hivep3 A G 4: 119,872,050 (GRCm38) probably benign Het
Hk2 C T 6: 82,739,650 (GRCm38) M300I possibly damaging Het
Ighv1-61 T C 12: 115,359,279 (GRCm38) Y71C probably damaging Het
Il1f8 T C 2: 24,154,618 (GRCm38) V10A probably benign Het
Inpp5f G T 7: 128,663,987 (GRCm38) S152I probably damaging Het
Jag1 C A 2: 137,096,309 (GRCm38) W257L probably damaging Het
Jak2 T A 19: 29,294,915 (GRCm38) N612K possibly damaging Het
Kalrn T C 16: 34,203,957 (GRCm38) D610G probably damaging Het
Kbtbd6 A G 14: 79,452,606 (GRCm38) D247G probably benign Het
Kif15 A G 9: 122,959,993 (GRCm38) probably null Het
Kndc1 A G 7: 139,930,123 (GRCm38) T1293A possibly damaging Het
Lpar5 T C 6: 125,082,207 (GRCm38) I297T possibly damaging Het
Lpin2 T A 17: 71,232,143 (GRCm38) probably benign Het
Mfsd4a A T 1: 132,053,571 (GRCm38) L230Q probably damaging Het
Mmp8 T C 9: 7,565,549 (GRCm38) V313A probably benign Het
Mrpl19 A T 6: 81,964,285 (GRCm38) D98E probably damaging Het
Mybl1 A G 1: 9,690,115 (GRCm38) I86T probably damaging Het
Nadk C A 4: 155,585,227 (GRCm38) P157T probably benign Het
Necab1 T C 4: 15,052,628 (GRCm38) T117A probably damaging Het
Nol11 A G 11: 107,184,718 (GRCm38) probably benign Het
Nucb2 G A 7: 116,540,027 (GRCm38) probably null Het
Nupl1 G T 14: 60,251,215 (GRCm38) P19T unknown Het
Odf2 T A 2: 29,904,034 (GRCm38) L301Q probably damaging Het
Oit1 T C 14: 8,349,347 (GRCm38) E201G probably benign Het
Olfr374 T A 8: 72,110,200 (GRCm38) F211L probably damaging Het
Olfr736 A G 14: 50,392,800 (GRCm38) I15V probably benign Het
Oog4 T C 4: 143,438,875 (GRCm38) Y234C probably benign Het
Papln T C 12: 83,777,208 (GRCm38) probably null Het
Paqr6 C T 3: 88,365,929 (GRCm38) A76V probably benign Het
Pclo A G 5: 14,676,480 (GRCm38) probably benign Het
Pdzd8 T C 19: 59,345,311 (GRCm38) T93A possibly damaging Het
Pkdrej A T 15: 85,821,167 (GRCm38) Y189* probably null Het
Pknox2 A T 9: 36,923,638 (GRCm38) N178K possibly damaging Het
Plxnd1 A C 6: 115,958,620 (GRCm38) L1735R probably damaging Het
Polm T A 11: 5,837,663 (GRCm38) D30V possibly damaging Het
Rap1gap2 T A 11: 74,437,439 (GRCm38) I100F probably damaging Het
Rasgef1c T A 11: 49,978,467 (GRCm38) W414R probably benign Het
Rassf1 A G 9: 107,557,867 (GRCm38) D187G probably benign Het
Rhag T C 17: 40,836,438 (GRCm38) I397T probably benign Het
Rnft2 A G 5: 118,228,863 (GRCm38) F269S probably damaging Het
Rnmt T C 18: 68,314,125 (GRCm38) F360S probably damaging Het
Ror2 C T 13: 53,117,297 (GRCm38) A329T probably benign Het
Slc4a1 T A 11: 102,356,258 (GRCm38) N501I possibly damaging Het
Slc4a7 T A 14: 14,733,856 (GRCm38) S89T probably damaging Het
Sptbn1 G A 11: 30,100,660 (GRCm38) H2310Y probably benign Het
Tbc1d9b C A 11: 50,140,462 (GRCm38) N103K probably benign Het
Tbxas1 T C 6: 39,083,857 (GRCm38) probably null Het
Tmem100 C T 11: 90,035,563 (GRCm38) T72I probably damaging Het
Ttc38 A G 15: 85,852,963 (GRCm38) T350A probably benign Het
Ubr4 C T 4: 139,450,529 (GRCm38) T3241M probably damaging Het
Unc13d A T 11: 116,073,388 (GRCm38) M350K possibly damaging Het
Vit T C 17: 78,625,114 (GRCm38) I550T probably damaging Het
Vmn1r31 C A 6: 58,471,968 (GRCm38) *304L probably null Het
Zbtb12 T A 17: 34,896,401 (GRCm38) H387Q possibly damaging Het
Other mutations in Pla2g15
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01522:Pla2g15 APN 8 106,163,116 (GRCm38) missense probably benign 0.00
IGL02719:Pla2g15 APN 8 106,160,196 (GRCm38) missense probably benign 0.01
IGL03065:Pla2g15 APN 8 106,160,219 (GRCm38) missense probably benign 0.20
R0125:Pla2g15 UTSW 8 106,163,124 (GRCm38) missense probably benign 0.00
R1691:Pla2g15 UTSW 8 106,154,949 (GRCm38) missense possibly damaging 0.93
R1939:Pla2g15 UTSW 8 106,163,295 (GRCm38) missense probably damaging 0.96
R3887:Pla2g15 UTSW 8 106,161,135 (GRCm38) missense probably damaging 0.99
R4633:Pla2g15 UTSW 8 106,160,255 (GRCm38) missense probably damaging 1.00
R4702:Pla2g15 UTSW 8 106,163,059 (GRCm38) missense probably benign 0.08
R4703:Pla2g15 UTSW 8 106,163,059 (GRCm38) missense probably benign 0.08
R4766:Pla2g15 UTSW 8 106,163,071 (GRCm38) missense probably damaging 1.00
R4928:Pla2g15 UTSW 8 106,163,218 (GRCm38) nonsense probably null
R5543:Pla2g15 UTSW 8 106,161,143 (GRCm38) missense probably damaging 0.99
R6489:Pla2g15 UTSW 8 106,163,194 (GRCm38) missense probably benign 0.10
R6802:Pla2g15 UTSW 8 106,150,581 (GRCm38) missense probably damaging 0.99
R7381:Pla2g15 UTSW 8 106,162,944 (GRCm38) missense probably benign 0.03
R9245:Pla2g15 UTSW 8 106,162,907 (GRCm38) missense possibly damaging 0.81
R9302:Pla2g15 UTSW 8 106,150,569 (GRCm38) missense probably benign 0.04
R9623:Pla2g15 UTSW 8 106,160,643 (GRCm38) missense possibly damaging 0.90
Z1177:Pla2g15 UTSW 8 106,162,987 (GRCm38) missense probably benign 0.19
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2015-10-21