Mutagenetix > Incidental Mutation

Incidental Mutation 'R4707:Fras1'

355310

Institutional Source

Beutler Lab

Gene Symbol

Fras1

Ensembl Gene

ENSMUSG00000034687

Gene Name

Fraser extracellular matrix complex subunit 1

Synonyms

bl, E130113P14Rik

MMRRC Submission

041955-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4707 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

96373955-96784728 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 96735238 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 2543 (N2543S)

Ref Sequence

ENSEMBL: ENSMUSP00000043250 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036019]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000036019
AA Change: N2543S

PolyPhen 2 Score 0.964 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000043250
Gene: ENSMUSG00000034687
AA Change: N2543S

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
VWC	27	86	9.23e-9	SMART
VWC	94	151	9.37e-10	SMART
VWC	158	215	8.61e-9	SMART
VWC	220	277	7.1e-10	SMART
VWC	284	341	7.8e-7	SMART
VWC	365	415	1.93e-1	SMART
FU	408	459	3.33e-1	SMART
FU	461	504	9.12e-8	SMART
EGF_like	466	495	6.67e1	SMART
FU	506	552	6.01e-8	SMART
FU	554	598	2.21e-6	SMART
FU	601	646	1.28e-11	SMART
FU	648	704	4.19e-7	SMART
FU	707	752	9.12e-8	SMART
FU	754	799	1.11e-6	SMART
EGF_like	759	790	7.23e1	SMART
FU	802	851	5.44e-6	SMART
EGF_like	807	842	4.55e1	SMART
FU	853	899	7.4e-8	SMART
FU	902	947	4.78e-2	SMART
FU	951	996	4.52e-12	SMART
EGF_like	956	987	2.75e1	SMART
FU	998	1041	1.38e-7	SMART
FU	1045	1088	9.7e-3	SMART
EGF_like	1057	1096	3.16e1	SMART
Pfam:Cadherin_3	1098	1198	5.2e-12	PFAM
Pfam:Cadherin_3	1167	1309	6.5e-27	PFAM
Pfam:Cadherin_3	1278	1442	7e-24	PFAM
Pfam:Cadherin_3	1411	1560	1.3e-23	PFAM
Pfam:Cadherin_3	1561	1693	6.4e-15	PFAM
Pfam:Cadherin_3	1695	1814	1.1e-10	PFAM
Pfam:Cadherin_3	1780	1940	1.6e-18	PFAM
Pfam:Cadherin_3	1906	2061	2.8e-22	PFAM
Pfam:Cadherin_3	2063	2181	3.3e-18	PFAM
Pfam:Cadherin_3	2172	2295	1.4e-26	PFAM
Pfam:Cadherin_3	2296	2408	2.3e-31	PFAM
Pfam:Cadherin_3	2413	2540	7.9e-23	PFAM
Calx_beta	2544	2648	1.23e-10	SMART
Calx_beta	2661	2772	3.3e-11	SMART
Calx_beta	2787	2892	1.21e-9	SMART
Calx_beta	2907	3009	4.45e-3	SMART
Calx_beta	3027	3131	1.5e-14	SMART
Blast:Calx_beta	3162	3187	1e-5	BLAST
transmembrane domain	3902	3924	N/A	INTRINSIC
low complexity region	3927	3935	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200077

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein that appears to function in the regulation of epidermal-basement membrane adhesion and organogenesis during development. Mutations in this gene cause Fraser syndrome, a multisystem malformation that can include craniofacial, urogenital and respiratory system abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Survival is variable on genetic backgrounds. Kidney development is severely affected and syndactyly is common. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 115 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010I14Rik	A	G	17: 9,005,712 (GRCm38)	K450R	probably damaging	Het
Abcd2	T	C	15: 91,159,182 (GRCm38)	D601G	probably benign	Het
Abcf3	A	G	16: 20,549,058 (GRCm38)	K56E	possibly damaging	Het
Acaca	T	C	11: 84,312,854 (GRCm38)	V1477A	probably damaging	Het
Adamts20	G	A	15: 94,333,647 (GRCm38)	P887L	possibly damaging	Het
Ahnak	T	G	19: 9,016,735 (GRCm38)	S5128A	probably benign	Het
Ahsa2	C	A	11: 23,493,162 (GRCm38)	V197F	probably benign	Het
Ak9	C	A	10: 41,345,460 (GRCm38)	H402N	probably benign	Het
Alpk1	G	T	3: 127,687,592 (GRCm38)	N175K	possibly damaging	Het
Als2	A	G	1: 59,215,313 (GRCm38)	V295A	probably benign	Het
Ankrd16	A	G	2: 11,778,797 (GRCm38)	D70G	probably damaging	Het
Apob	A	T	12: 8,006,205 (GRCm38)	K1562N	probably damaging	Het
Arfgap2	C	A	2: 91,269,971 (GRCm38)	S250R	probably damaging	Het
Arhgef10l	C	T	4: 140,536,883 (GRCm38)	M671I	possibly damaging	Het
Atrnl1	A	G	19: 57,629,158 (GRCm38)	I122V	probably damaging	Het
B4galnt2	C	T	11: 95,876,097 (GRCm38)		probably null	Het
C8a	A	G	4: 104,856,421 (GRCm38)	Y171H	probably damaging	Het
Cacnb4	A	G	2: 52,474,915 (GRCm38)	V112A	probably benign	Het
Capn9	G	T	8: 124,613,456 (GRCm38)	C566F	possibly damaging	Het
Ccdc88a	T	A	11: 29,447,956 (GRCm38)	S230T	probably benign	Het
Cd300c2	A	T	11: 114,996,985 (GRCm38)	F197Y	probably benign	Het
Chd5	A	G	4: 152,360,582 (GRCm38)	Y340C	probably damaging	Het
Chrd	A	T	16: 20,738,808 (GRCm38)	I726F	possibly damaging	Het
Chrna10	G	A	7: 102,113,219 (GRCm38)	P255S	possibly damaging	Het
Clasp1	T	A	1: 118,543,197 (GRCm38)	Y197*	probably null	Het
Cyp2c69	C	G	19: 39,849,408 (GRCm38)	G410A	probably benign	Het
Cyp2e1	G	A	7: 140,763,908 (GRCm38)	V20I	possibly damaging	Het
Dapp1	T	C	3: 137,933,167 (GRCm38)	D225G	probably benign	Het
Dnah5	A	T	15: 28,372,375 (GRCm38)	D2924V	probably damaging	Het
Efcab12	A	T	6: 115,814,549 (GRCm38)	L554Q	possibly damaging	Het
Emsy	T	C	7: 98,597,104 (GRCm38)	T228A	possibly damaging	Het
Evc2	G	A	5: 37,421,860 (GRCm38)	V1106I	probably benign	Het
Exoc8	G	T	8: 124,897,470 (GRCm38)	Q53K	possibly damaging	Het
Fam160a1	G	T	3: 85,688,570 (GRCm38)	T115K	probably damaging	Het
Fbn2	A	T	18: 58,056,272 (GRCm38)	V1594D	probably damaging	Het
Fer1l4	T	C	2: 156,045,623 (GRCm38)	Y551C	possibly damaging	Het
Fmnl3	A	G	15: 99,323,481 (GRCm38)	M481T	probably benign	Het
Fsd2	T	C	7: 81,559,680 (GRCm38)	D138G	probably damaging	Het
Gda	C	T	19: 21,428,628 (GRCm38)	V5I	probably benign	Het
Glt8d2	A	T	10: 82,660,749 (GRCm38)	D158E	probably damaging	Het
Gpx6	C	T	13: 21,312,264 (GRCm38)	Q3*	probably null	Het
Greb1l	A	G	18: 10,532,922 (GRCm38)	M830V	probably benign	Het
Hnrnpul1	A	C	7: 25,726,833 (GRCm38)	V531G	probably damaging	Het
Ifi206	A	T	1: 173,480,866 (GRCm38)	H521Q	probably benign	Het
Igsf3	G	C	3: 101,458,094 (GRCm38)	R1127P	probably benign	Het
Il1rl1	A	G	1: 40,450,188 (GRCm38)	R367G	probably damaging	Het
Islr	T	C	9: 58,157,687 (GRCm38)	D179G	possibly damaging	Het
Jcad	G	T	18: 4,649,338 (GRCm38)	E70*	probably null	Het
Kcnd2	T	C	6: 21,723,212 (GRCm38)	I467T	probably benign	Het
Lrrc27	A	G	7: 139,242,698 (GRCm38)	T502A	probably benign	Het
Lrrtm3	T	A	10: 64,088,002 (GRCm38)	H462L	probably benign	Het
Mbd5	T	C	2: 49,250,156 (GRCm38)	L44S	probably damaging	Het
Mccc1	C	A	3: 35,975,873 (GRCm38)	M429I	probably damaging	Het
Mgam	A	T	6: 40,714,632 (GRCm38)		probably null	Het
Mipep	T	A	14: 60,872,103 (GRCm38)	I643N	probably damaging	Het
Mmrn1	A	T	6: 60,988,473 (GRCm38)	I1162L	probably benign	Het
Mrc2	G	A	11: 105,348,431 (GRCm38)		probably null	Het
Mug1	G	A	6: 121,884,641 (GRCm38)	C1354Y	probably damaging	Het
Nbeal2	A	T	9: 110,632,055 (GRCm38)	S1647T	probably benign	Het
Ndufaf2	C	G	13: 108,052,780 (GRCm38)	A145P	probably damaging	Het
Nedd9	A	T	13: 41,338,575 (GRCm38)		probably null	Het
Nr4a2	T	A	2: 57,112,093 (GRCm38)	H116L	probably benign	Het
Nwd2	A	G	5: 63,794,322 (GRCm38)	Y232C	probably damaging	Het
Odf4	A	G	11: 68,926,688 (GRCm38)	L58P	probably damaging	Het
Olfr1197	T	A	2: 88,728,712 (GRCm38)	M296L	possibly damaging	Het
Olfr1221	A	T	2: 89,112,232 (GRCm38)	F93L	probably damaging	Het
Olfr1284	T	A	2: 111,379,645 (GRCm38)	L215H	probably damaging	Het
Olfr284	G	T	15: 98,340,778 (GRCm38)	H70Q	possibly damaging	Het
Olfr434	T	A	6: 43,216,949 (GRCm38)	V12D	probably benign	Het
Olfr58	C	T	9: 19,783,300 (GRCm38)	H18Y	probably damaging	Het
Olfr938	A	G	9: 39,078,262 (GRCm38)	V161A	probably benign	Het
Orc6	T	A	8: 85,302,950 (GRCm38)	I41K	probably damaging	Het
Pikfyve	A	G	1: 65,267,846 (GRCm38)	T1798A	probably benign	Het
Prdx6b	T	C	2: 80,293,060 (GRCm38)	L71P	probably damaging	Het
Prmt2	A	G	10: 76,226,221 (GRCm38)	I50T	probably damaging	Het
Ptdss1	T	C	13: 66,995,418 (GRCm38)		probably null	Het
Pygl	T	C	12: 70,207,758 (GRCm38)	T138A	possibly damaging	Het
Rab3c	T	C	13: 110,061,900 (GRCm38)	E198G	probably benign	Het
Rbl2	A	G	8: 91,085,568 (GRCm38)	Y255C	probably damaging	Het
Rev3l	T	A	10: 39,823,397 (GRCm38)	S1297T	probably damaging	Het
Rgma	C	A	7: 73,417,816 (GRCm38)	T367K	probably damaging	Het
Rps6ka5	C	T	12: 100,597,885 (GRCm38)		probably null	Het
Rslcan18	A	T	13: 67,098,526 (GRCm38)	C217S	probably damaging	Het
Rtp3	A	G	9: 110,986,211 (GRCm38)		probably benign	Het
Ryr1	A	T	7: 29,045,662 (GRCm38)	N3848K	probably damaging	Het
Sema6a	T	A	18: 47,248,712 (GRCm38)	T923S	probably benign	Het
Serpinb6d	A	T	13: 33,671,353 (GRCm38)	T337S	possibly damaging	Het
Sf3b1	G	A	1: 54,990,507 (GRCm38)	T1112M	probably damaging	Het
Shroom3	G	T	5: 92,943,086 (GRCm38)	V1151F	probably damaging	Het
Sidt1	A	T	16: 44,269,858 (GRCm38)	Y369*	probably null	Het
Slc16a6	A	G	11: 109,463,367 (GRCm38)	S59P	probably benign	Het
Slc25a17	A	C	15: 81,327,326 (GRCm38)	L163W	probably damaging	Het
Slc44a3	T	C	3: 121,527,074 (GRCm38)	T93A	possibly damaging	Het
Sptbn1	T	C	11: 30,137,197 (GRCm38)	T1081A	possibly damaging	Het
Sptbn4	T	C	7: 27,417,006 (GRCm38)	D456G	probably benign	Het
Sycp1	C	A	3: 102,853,489 (GRCm38)	A703S	possibly damaging	Het
Tas2r118	T	A	6: 23,969,226 (GRCm38)	M279L	probably benign	Het
Tc2n	T	G	12: 101,694,573 (GRCm38)	Q133H	probably benign	Het
Tctn1	A	G	5: 122,261,405 (GRCm38)		probably null	Het
Tenm4	A	C	7: 96,774,046 (GRCm38)	K683Q	probably damaging	Het
Tex10	A	T	4: 48,468,984 (GRCm38)	S64T	probably benign	Het
Tex15	A	G	8: 33,582,497 (GRCm38)	T2691A	probably benign	Het
Tmem175	A	T	5: 108,642,150 (GRCm38)	T123S	probably damaging	Het
Tsc1	C	A	2: 28,672,407 (GRCm38)	S348R	probably damaging	Het
Ttll4	A	T	1: 74,679,007 (GRCm38)	T6S	possibly damaging	Het
Ttll8	A	T	15: 88,917,090 (GRCm38)	I465N	probably damaging	Het
Ubr3	A	G	2: 69,938,370 (GRCm38)		probably benign	Het
Ugdh	A	T	5: 65,423,352 (GRCm38)		probably null	Het
Usp34	T	C	11: 23,487,215 (GRCm38)	L3326S	probably damaging	Het
Vmn2r125	A	T	4: 156,349,981 (GRCm38)	I21F	probably damaging	Het
Vps9d1	G	A	8: 123,248,612 (GRCm38)		probably benign	Het
Xab2	G	A	8: 3,618,117 (GRCm38)	R154C	possibly damaging	Het
Zbtb12	T	C	17: 34,895,499 (GRCm38)	S87P	probably damaging	Het
Zc3h12a	A	G	4: 125,120,893 (GRCm38)	M266T	probably damaging	Het
Zfp108	G	A	7: 24,260,412 (GRCm38)	A143T	probably benign	Het

Other mutations in Fras1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Fras1	APN	5	96,739,358 (GRCm38)	missense	possibly damaging	0.55
IGL00507:Fras1	APN	5	96,778,189 (GRCm38)	missense	probably damaging	1.00
IGL00672:Fras1	APN	5	96,759,450 (GRCm38)	splice site	probably benign
IGL00772:Fras1	APN	5	96,636,112 (GRCm38)	missense	probably benign	0.42
IGL00844:Fras1	APN	5	96,534,853 (GRCm38)	splice site	probably benign
IGL00913:Fras1	APN	5	96,695,076 (GRCm38)	missense	probably damaging	0.99
IGL00959:Fras1	APN	5	96,781,281 (GRCm38)	missense	probably damaging	0.96
IGL00966:Fras1	APN	5	96,555,221 (GRCm38)	missense	probably benign	0.00
IGL01296:Fras1	APN	5	96,673,698 (GRCm38)	missense	probably null	0.58
IGL01307:Fras1	APN	5	96,781,692 (GRCm38)	missense	probably benign
IGL01481:Fras1	APN	5	96,657,241 (GRCm38)	missense	probably damaging	1.00
IGL01525:Fras1	APN	5	96,739,336 (GRCm38)	missense	probably damaging	0.99
IGL01599:Fras1	APN	5	96,709,891 (GRCm38)	missense	possibly damaging	0.94
IGL01646:Fras1	APN	5	96,758,148 (GRCm38)	missense	probably benign	0.29
IGL01795:Fras1	APN	5	96,778,045 (GRCm38)	missense	probably damaging	1.00
IGL01867:Fras1	APN	5	96,588,131 (GRCm38)	missense	probably benign
IGL01869:Fras1	APN	5	96,708,783 (GRCm38)	splice site	probably benign
IGL01923:Fras1	APN	5	96,735,280 (GRCm38)	missense	probably damaging	1.00
IGL01982:Fras1	APN	5	96,739,248 (GRCm38)	missense	possibly damaging	0.46
IGL02109:Fras1	APN	5	96,700,523 (GRCm38)	missense	probably benign
IGL02132:Fras1	APN	5	96,781,637 (GRCm38)	nonsense	probably null
IGL02171:Fras1	APN	5	96,735,181 (GRCm38)	missense	probably benign	0.15
IGL02213:Fras1	APN	5	96,645,871 (GRCm38)	nonsense	probably null
IGL02277:Fras1	APN	5	96,588,118 (GRCm38)	missense	probably benign	0.00
IGL02507:Fras1	APN	5	96,657,408 (GRCm38)	missense	possibly damaging	0.95
IGL02589:Fras1	APN	5	96,769,513 (GRCm38)	missense	probably damaging	1.00
IGL02671:Fras1	APN	5	96,728,616 (GRCm38)	missense	possibly damaging	0.91
IGL02677:Fras1	APN	5	96,545,024 (GRCm38)	missense	probably damaging	1.00
IGL02691:Fras1	APN	5	96,744,705 (GRCm38)	missense	possibly damaging	0.68
IGL02741:Fras1	APN	5	96,691,371 (GRCm38)	missense	probably benign	0.35
IGL02836:Fras1	APN	5	96,534,866 (GRCm38)	missense	possibly damaging	0.67
IGL02850:Fras1	APN	5	96,778,175 (GRCm38)	missense	probably damaging	1.00
IGL02998:Fras1	APN	5	96,702,181 (GRCm38)	missense	possibly damaging	0.82
IGL03040:Fras1	APN	5	96,710,101 (GRCm38)	missense	probably benign
IGL03078:Fras1	APN	5	96,636,135 (GRCm38)	missense	probably damaging	1.00
IGL03096:Fras1	APN	5	96,764,901 (GRCm38)	missense	probably damaging	1.00
IGL03102:Fras1	APN	5	96,726,535 (GRCm38)	missense	probably benign	0.11
IGL03183:Fras1	APN	5	96,733,781 (GRCm38)	splice site	probably benign
IGL03189:Fras1	APN	5	96,743,071 (GRCm38)	missense	probably benign	0.00
IGL03193:Fras1	APN	5	96,778,106 (GRCm38)	missense	probably damaging	0.99
IGL03292:Fras1	APN	5	96,707,491 (GRCm38)	missense	probably damaging	1.00
IGL03328:Fras1	APN	5	96,781,760 (GRCm38)	missense	probably damaging	0.96
IGL03335:Fras1	APN	5	96,733,944 (GRCm38)	splice site	probably benign
IGL03394:Fras1	APN	5	96,667,477 (GRCm38)	missense	probably damaging	0.98
IGL03404:Fras1	APN	5	96,728,581 (GRCm38)	missense	probably damaging	0.99
baby_ruth	UTSW	5	96,708,758 (GRCm38)	missense	probably benign	0.01
BB002:Fras1	UTSW	5	96,781,584 (GRCm38)	missense	probably damaging	0.96
BB012:Fras1	UTSW	5	96,781,584 (GRCm38)	missense	probably damaging	0.96
G1patch:Fras1	UTSW	5	96,781,340 (GRCm38)	missense	possibly damaging	0.91
I0000:Fras1	UTSW	5	96,740,829 (GRCm38)	missense	probably damaging	0.99
PIT4581001:Fras1	UTSW	5	96,555,301 (GRCm38)	missense	probably benign	0.01
R0028:Fras1	UTSW	5	96,677,316 (GRCm38)	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96,776,622 (GRCm38)	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96,776,622 (GRCm38)	missense	probably benign	0.07
R0099:Fras1	UTSW	5	96,614,917 (GRCm38)	critical splice donor site	probably null
R0109:Fras1	UTSW	5	96,710,077 (GRCm38)	missense	probably benign	0.01
R0158:Fras1	UTSW	5	96,776,634 (GRCm38)	missense	possibly damaging	0.83
R0268:Fras1	UTSW	5	96,737,009 (GRCm38)	missense	probably damaging	0.99
R0305:Fras1	UTSW	5	96,596,888 (GRCm38)	missense	probably benign
R0352:Fras1	UTSW	5	96,726,540 (GRCm38)	missense	probably damaging	0.97
R0359:Fras1	UTSW	5	96,762,590 (GRCm38)	missense	probably damaging	0.98
R0371:Fras1	UTSW	5	96,555,331 (GRCm38)	missense	possibly damaging	0.90
R0379:Fras1	UTSW	5	96,755,509 (GRCm38)	nonsense	probably null
R0395:Fras1	UTSW	5	96,769,653 (GRCm38)	missense	possibly damaging	0.50
R0417:Fras1	UTSW	5	96,691,372 (GRCm38)	missense	probably benign	0.18
R0454:Fras1	UTSW	5	96,762,665 (GRCm38)	missense	probably damaging	0.96
R0456:Fras1	UTSW	5	96,714,343 (GRCm38)	splice site	probably null
R0456:Fras1	UTSW	5	96,554,788 (GRCm38)	missense	probably damaging	1.00
R0464:Fras1	UTSW	5	96,636,803 (GRCm38)	missense	probably damaging	0.98
R0613:Fras1	UTSW	5	96,700,488 (GRCm38)	splice site	probably benign
R0652:Fras1	UTSW	5	96,781,340 (GRCm38)	missense	possibly damaging	0.91
R0675:Fras1	UTSW	5	96,667,387 (GRCm38)	splice site	probably benign
R0765:Fras1	UTSW	5	96,552,796 (GRCm38)	missense	probably benign	0.00
R0783:Fras1	UTSW	5	96,768,430 (GRCm38)	missense	probably damaging	1.00
R0811:Fras1	UTSW	5	96,752,998 (GRCm38)	missense	probably benign	0.35
R0812:Fras1	UTSW	5	96,752,998 (GRCm38)	missense	probably benign	0.35
R0943:Fras1	UTSW	5	96,726,543 (GRCm38)	missense	probably benign	0.00
R1037:Fras1	UTSW	5	96,714,463 (GRCm38)	missense	probably damaging	0.97
R1104:Fras1	UTSW	5	96,708,671 (GRCm38)	missense	probably benign	0.00
R1108:Fras1	UTSW	5	96,642,629 (GRCm38)	missense	probably damaging	0.99
R1332:Fras1	UTSW	5	96,707,308 (GRCm38)	missense	probably benign	0.00
R1336:Fras1	UTSW	5	96,707,308 (GRCm38)	missense	probably benign	0.00
R1458:Fras1	UTSW	5	96,600,733 (GRCm38)	missense	probably benign	0.00
R1495:Fras1	UTSW	5	96,528,586 (GRCm38)	missense	possibly damaging	0.49
R1499:Fras1	UTSW	5	96,743,187 (GRCm38)	missense	probably benign	0.31
R1528:Fras1	UTSW	5	96,636,819 (GRCm38)	missense	probably damaging	0.99
R1532:Fras1	UTSW	5	96,713,996 (GRCm38)	missense	probably damaging	1.00
R1556:Fras1	UTSW	5	96,743,062 (GRCm38)	missense	possibly damaging	0.88
R1625:Fras1	UTSW	5	96,709,978 (GRCm38)	missense	possibly damaging	0.94
R1625:Fras1	UTSW	5	96,713,990 (GRCm38)	missense	probably damaging	1.00
R1645:Fras1	UTSW	5	96,700,586 (GRCm38)	missense	possibly damaging	0.90
R1647:Fras1	UTSW	5	96,726,613 (GRCm38)	critical splice donor site	probably null
R1648:Fras1	UTSW	5	96,726,613 (GRCm38)	critical splice donor site	probably null
R1661:Fras1	UTSW	5	96,598,909 (GRCm38)	missense	probably damaging	1.00
R1665:Fras1	UTSW	5	96,598,909 (GRCm38)	missense	probably damaging	1.00
R1682:Fras1	UTSW	5	96,645,873 (GRCm38)	missense	probably benign	0.00
R1701:Fras1	UTSW	5	96,600,784 (GRCm38)	missense	probably benign	0.00
R1716:Fras1	UTSW	5	96,552,725 (GRCm38)	missense	probably benign	0.10
R1718:Fras1	UTSW	5	96,554,889 (GRCm38)	splice site	probably null
R1800:Fras1	UTSW	5	96,709,882 (GRCm38)	missense	probably benign
R1806:Fras1	UTSW	5	96,764,976 (GRCm38)	missense	possibly damaging	0.88
R1806:Fras1	UTSW	5	96,713,970 (GRCm38)	splice site	probably benign
R1822:Fras1	UTSW	5	96,770,688 (GRCm38)	missense	probably damaging	1.00
R1823:Fras1	UTSW	5	96,770,688 (GRCm38)	missense	probably damaging	1.00
R1824:Fras1	UTSW	5	96,770,688 (GRCm38)	missense	probably damaging	1.00
R1847:Fras1	UTSW	5	96,749,423 (GRCm38)	splice site	probably null
R1929:Fras1	UTSW	5	96,667,437 (GRCm38)	missense	probably benign	0.24
R1951:Fras1	UTSW	5	96,712,383 (GRCm38)	missense	probably benign	0.38
R2093:Fras1	UTSW	5	96,781,203 (GRCm38)	missense	probably damaging	1.00
R2283:Fras1	UTSW	5	96,654,305 (GRCm38)	missense	probably benign	0.10
R2884:Fras1	UTSW	5	96,700,268 (GRCm38)	missense	probably benign	0.07
R2913:Fras1	UTSW	5	96,733,915 (GRCm38)	missense	probably benign
R2914:Fras1	UTSW	5	96,733,915 (GRCm38)	missense	probably benign
R3054:Fras1	UTSW	5	96,764,943 (GRCm38)	missense	probably damaging	0.99
R3117:Fras1	UTSW	5	96,771,712 (GRCm38)	missense	probably damaging	1.00
R3118:Fras1	UTSW	5	96,771,712 (GRCm38)	missense	probably damaging	1.00
R3691:Fras1	UTSW	5	96,781,512 (GRCm38)	missense	probably benign	0.02
R3714:Fras1	UTSW	5	96,645,970 (GRCm38)	critical splice donor site	probably null
R3715:Fras1	UTSW	5	96,645,970 (GRCm38)	critical splice donor site	probably null
R3801:Fras1	UTSW	5	96,733,932 (GRCm38)	missense	probably benign	0.26
R3961:Fras1	UTSW	5	96,677,385 (GRCm38)	critical splice donor site	probably null
R4065:Fras1	UTSW	5	96,770,683 (GRCm38)	missense	possibly damaging	0.64
R4066:Fras1	UTSW	5	96,770,683 (GRCm38)	missense	possibly damaging	0.64
R4076:Fras1	UTSW	5	96,743,158 (GRCm38)	missense	probably damaging	1.00
R4124:Fras1	UTSW	5	96,770,653 (GRCm38)	missense	probably benign	0.05
R4127:Fras1	UTSW	5	96,770,653 (GRCm38)	missense	probably benign	0.05
R4153:Fras1	UTSW	5	96,776,735 (GRCm38)	missense	probably benign	0.17
R4233:Fras1	UTSW	5	96,714,376 (GRCm38)	missense	possibly damaging	0.91
R4273:Fras1	UTSW	5	96,614,904 (GRCm38)	missense	probably benign	0.00
R4355:Fras1	UTSW	5	96,700,242 (GRCm38)	missense	probably benign
R4401:Fras1	UTSW	5	96,642,620 (GRCm38)	missense	probably damaging	0.97
R4402:Fras1	UTSW	5	96,642,620 (GRCm38)	missense	probably damaging	0.97
R4403:Fras1	UTSW	5	96,642,620 (GRCm38)	missense	probably damaging	0.97
R4505:Fras1	UTSW	5	96,781,348 (GRCm38)	missense	probably damaging	1.00
R4548:Fras1	UTSW	5	96,709,895 (GRCm38)	missense	probably benign	0.00
R4559:Fras1	UTSW	5	96,781,289 (GRCm38)	missense	probably damaging	1.00
R4629:Fras1	UTSW	5	96,776,734 (GRCm38)	missense	probably benign	0.00
R4637:Fras1	UTSW	5	96,778,088 (GRCm38)	missense	probably damaging	1.00
R4678:Fras1	UTSW	5	96,700,568 (GRCm38)	missense	probably benign	0.13
R4735:Fras1	UTSW	5	96,588,163 (GRCm38)	missense	probably benign	0.00
R4756:Fras1	UTSW	5	96,781,659 (GRCm38)	missense	probably benign	0.00
R4762:Fras1	UTSW	5	96,731,618 (GRCm38)	missense	probably benign
R4820:Fras1	UTSW	5	96,728,653 (GRCm38)	missense	probably benign	0.00
R4847:Fras1	UTSW	5	96,544,992 (GRCm38)	missense	possibly damaging	0.94
R4857:Fras1	UTSW	5	96,778,159 (GRCm38)	missense	probably benign	0.00
R4909:Fras1	UTSW	5	96,708,758 (GRCm38)	missense	probably benign	0.01
R4931:Fras1	UTSW	5	96,636,840 (GRCm38)	missense	probably benign	0.02
R4938:Fras1	UTSW	5	96,776,724 (GRCm38)	missense	probably damaging	0.99
R4952:Fras1	UTSW	5	96,647,498 (GRCm38)	missense	probably benign	0.01
R4965:Fras1	UTSW	5	96,726,580 (GRCm38)	missense	possibly damaging	0.95
R4989:Fras1	UTSW	5	96,650,682 (GRCm38)	missense	possibly damaging	0.75
R5151:Fras1	UTSW	5	96,645,110 (GRCm38)	missense	probably damaging	1.00
R5168:Fras1	UTSW	5	96,708,757 (GRCm38)	missense	probably benign	0.00
R5182:Fras1	UTSW	5	96,636,173 (GRCm38)	nonsense	probably null
R5214:Fras1	UTSW	5	96,769,593 (GRCm38)	missense	probably damaging	1.00
R5220:Fras1	UTSW	5	96,768,363 (GRCm38)	missense	probably damaging	1.00
R5235:Fras1	UTSW	5	96,600,750 (GRCm38)	missense	probably benign	0.02
R5242:Fras1	UTSW	5	96,657,250 (GRCm38)	missense	probably benign	0.11
R5253:Fras1	UTSW	5	96,741,025 (GRCm38)	missense	probably damaging	0.99
R5260:Fras1	UTSW	5	96,735,187 (GRCm38)	missense	possibly damaging	0.79
R5301:Fras1	UTSW	5	96,657,266 (GRCm38)	missense	possibly damaging	0.88
R5411:Fras1	UTSW	5	96,645,160 (GRCm38)	missense	probably benign	0.00
R5467:Fras1	UTSW	5	96,780,053 (GRCm38)	missense	probably benign	0.04
R5543:Fras1	UTSW	5	96,528,535 (GRCm38)	missense	probably benign	0.01
R5555:Fras1	UTSW	5	96,677,377 (GRCm38)	missense	probably benign	0.34
R5602:Fras1	UTSW	5	96,737,021 (GRCm38)	missense	probably damaging	1.00
R5664:Fras1	UTSW	5	96,728,535 (GRCm38)	missense	possibly damaging	0.91
R5695:Fras1	UTSW	5	96,781,344 (GRCm38)	missense	probably damaging	1.00
R5717:Fras1	UTSW	5	96,781,737 (GRCm38)	missense	possibly damaging	0.93
R5742:Fras1	UTSW	5	96,768,381 (GRCm38)	missense	possibly damaging	0.50
R5759:Fras1	UTSW	5	96,709,916 (GRCm38)	missense	probably benign	0.02
R5766:Fras1	UTSW	5	96,731,689 (GRCm38)	missense	possibly damaging	0.91
R5890:Fras1	UTSW	5	96,645,948 (GRCm38)	missense	probably benign
R6052:Fras1	UTSW	5	96,764,866 (GRCm38)	missense	probably damaging	1.00
R6058:Fras1	UTSW	5	96,709,985 (GRCm38)	missense	probably benign
R6256:Fras1	UTSW	5	96,733,843 (GRCm38)	missense	possibly damaging	0.84
R6306:Fras1	UTSW	5	96,764,946 (GRCm38)	missense	probably damaging	1.00
R6494:Fras1	UTSW	5	96,759,564 (GRCm38)	missense	possibly damaging	0.59
R6638:Fras1	UTSW	5	96,758,094 (GRCm38)	missense	possibly damaging	0.94
R6647:Fras1	UTSW	5	96,735,202 (GRCm38)	missense	probably damaging	1.00
R6725:Fras1	UTSW	5	96,781,340 (GRCm38)	missense	possibly damaging	0.91
R6769:Fras1	UTSW	5	96,598,941 (GRCm38)	missense	possibly damaging	0.60
R6771:Fras1	UTSW	5	96,598,941 (GRCm38)	missense	possibly damaging	0.60
R6837:Fras1	UTSW	5	96,726,973 (GRCm38)	missense	probably damaging	0.99
R6841:Fras1	UTSW	5	96,728,551 (GRCm38)	missense	probably damaging	0.99
R6863:Fras1	UTSW	5	96,543,306 (GRCm38)	missense	probably benign	0.19
R6868:Fras1	UTSW	5	96,682,378 (GRCm38)	missense	probably benign	0.38
R6936:Fras1	UTSW	5	96,768,352 (GRCm38)	missense	possibly damaging	0.92
R6997:Fras1	UTSW	5	96,614,873 (GRCm38)	nonsense	probably null
R7023:Fras1	UTSW	5	96,710,084 (GRCm38)	missense	probably benign	0.00
R7091:Fras1	UTSW	5	96,708,676 (GRCm38)	missense	probably benign
R7102:Fras1	UTSW	5	96,571,041 (GRCm38)	missense	probably benign
R7120:Fras1	UTSW	5	96,752,960 (GRCm38)	nonsense	probably null
R7124:Fras1	UTSW	5	96,714,401 (GRCm38)	missense	probably damaging	1.00
R7129:Fras1	UTSW	5	96,781,284 (GRCm38)	missense	probably benign	0.00
R7173:Fras1	UTSW	5	96,778,078 (GRCm38)	missense	probably damaging	1.00
R7174:Fras1	UTSW	5	96,755,577 (GRCm38)	critical splice donor site	probably null
R7185:Fras1	UTSW	5	96,636,776 (GRCm38)	missense	probably damaging	1.00
R7191:Fras1	UTSW	5	96,614,912 (GRCm38)	missense	probably benign	0.05
R7216:Fras1	UTSW	5	96,739,314 (GRCm38)	missense	probably damaging	1.00
R7222:Fras1	UTSW	5	96,636,809 (GRCm38)	missense	probably benign	0.00
R7222:Fras1	UTSW	5	96,636,186 (GRCm38)	missense	probably damaging	1.00
R7320:Fras1	UTSW	5	96,709,886 (GRCm38)	missense	probably benign	0.03
R7335:Fras1	UTSW	5	96,736,970 (GRCm38)	missense	possibly damaging	0.82
R7378:Fras1	UTSW	5	96,596,785 (GRCm38)	missense	probably damaging	0.98
R7394:Fras1	UTSW	5	96,712,450 (GRCm38)	nonsense	probably null
R7412:Fras1	UTSW	5	96,614,889 (GRCm38)	missense	probably benign	0.06
R7422:Fras1	UTSW	5	96,673,599 (GRCm38)	missense	probably benign	0.21
R7552:Fras1	UTSW	5	96,768,438 (GRCm38)	missense	probably damaging	1.00
R7559:Fras1	UTSW	5	96,740,854 (GRCm38)	missense	possibly damaging	0.82
R7575:Fras1	UTSW	5	96,543,314 (GRCm38)	missense	probably benign	0.02
R7578:Fras1	UTSW	5	96,684,437 (GRCm38)	missense	probably damaging	1.00
R7600:Fras1	UTSW	5	96,684,436 (GRCm38)	missense	probably damaging	1.00
R7669:Fras1	UTSW	5	96,692,624 (GRCm38)	missense	probably benign	0.01
R7710:Fras1	UTSW	5	96,645,103 (GRCm38)	nonsense	probably null
R7722:Fras1	UTSW	5	96,769,554 (GRCm38)	missense	probably damaging	1.00
R7726:Fras1	UTSW	5	96,712,451 (GRCm38)	missense	probably benign	0.41
R7745:Fras1	UTSW	5	96,726,895 (GRCm38)	missense	probably benign	0.11
R7777:Fras1	UTSW	5	96,752,904 (GRCm38)	missense	probably damaging	1.00
R7923:Fras1	UTSW	5	96,739,318 (GRCm38)	missense	probably damaging	1.00
R7925:Fras1	UTSW	5	96,781,584 (GRCm38)	missense	probably damaging	0.96
R8000:Fras1	UTSW	5	96,762,677 (GRCm38)	missense	probably damaging	0.96
R8056:Fras1	UTSW	5	96,744,774 (GRCm38)	missense	probably damaging	1.00
R8058:Fras1	UTSW	5	96,694,919 (GRCm38)	missense	probably benign
R8117:Fras1	UTSW	5	96,707,386 (GRCm38)	missense	probably damaging	1.00
R8157:Fras1	UTSW	5	96,554,855 (GRCm38)	missense	probably benign	0.00
R8312:Fras1	UTSW	5	96,588,191 (GRCm38)	missense	probably benign
R8315:Fras1	UTSW	5	96,743,182 (GRCm38)	missense	probably damaging	0.97
R8412:Fras1	UTSW	5	96,596,852 (GRCm38)	missense	probably benign	0.00
R8546:Fras1	UTSW	5	96,709,966 (GRCm38)	missense	probably benign	0.00
R8705:Fras1	UTSW	5	96,691,401 (GRCm38)	missense	probably benign	0.01
R8790:Fras1	UTSW	5	96,755,377 (GRCm38)	missense	probably damaging	1.00
R8848:Fras1	UTSW	5	96,781,348 (GRCm38)	missense	probably damaging	1.00
R8855:Fras1	UTSW	5	96,769,606 (GRCm38)	missense
R8871:Fras1	UTSW	5	96,707,398 (GRCm38)	missense	probably benign	0.34
R8894:Fras1	UTSW	5	96,759,543 (GRCm38)	missense	probably damaging	1.00
R8904:Fras1	UTSW	5	96,781,279 (GRCm38)	missense	probably benign	0.16
R8910:Fras1	UTSW	5	96,567,996 (GRCm38)	missense	probably benign	0.01
R8916:Fras1	UTSW	5	96,752,915 (GRCm38)	missense	probably damaging	1.00
R8929:Fras1	UTSW	5	96,769,507 (GRCm38)	missense	probably damaging	0.98
R8995:Fras1	UTSW	5	96,712,556 (GRCm38)	missense	possibly damaging	0.83
R9016:Fras1	UTSW	5	96,636,064 (GRCm38)	missense	probably damaging	0.96
R9021:Fras1	UTSW	5	96,740,750 (GRCm38)	missense	probably damaging	1.00
R9038:Fras1	UTSW	5	96,726,883 (GRCm38)	missense	probably benign	0.25
R9204:Fras1	UTSW	5	96,735,163 (GRCm38)	missense	probably damaging	1.00
R9222:Fras1	UTSW	5	96,657,228 (GRCm38)	missense	probably benign	0.15
R9231:Fras1	UTSW	5	96,545,045 (GRCm38)	missense	probably damaging	1.00
R9238:Fras1	UTSW	5	96,684,361 (GRCm38)	missense	possibly damaging	0.90
R9257:Fras1	UTSW	5	96,762,500 (GRCm38)	missense	probably damaging	1.00
R9302:Fras1	UTSW	5	96,534,892 (GRCm38)	missense	probably damaging	1.00
R9329:Fras1	UTSW	5	96,736,954 (GRCm38)	missense	probably damaging	1.00
R9361:Fras1	UTSW	5	96,776,698 (GRCm38)	missense	probably damaging	1.00
R9430:Fras1	UTSW	5	96,781,392 (GRCm38)	missense	probably benign	0.00
R9431:Fras1	UTSW	5	96,753,014 (GRCm38)	missense	possibly damaging	0.71
R9474:Fras1	UTSW	5	96,739,265 (GRCm38)	missense	probably benign	0.00
R9475:Fras1	UTSW	5	96,780,070 (GRCm38)	missense	probably damaging	0.96
R9497:Fras1	UTSW	5	96,737,036 (GRCm38)	missense	probably damaging	1.00
R9597:Fras1	UTSW	5	96,740,692 (GRCm38)	missense	probably damaging	1.00
R9650:Fras1	UTSW	5	96,762,528 (GRCm38)	missense	probably damaging	1.00
R9694:Fras1	UTSW	5	96,781,686 (GRCm38)	missense	probably benign	0.03
R9779:Fras1	UTSW	5	96,569,494 (GRCm38)	missense	probably damaging	0.99
Z1088:Fras1	UTSW	5	96,743,211 (GRCm38)	missense	probably damaging	0.97
Z1088:Fras1	UTSW	5	96,758,142 (GRCm38)	missense	probably benign
Z1176:Fras1	UTSW	5	96,758,142 (GRCm38)	missense	probably benign
Z1177:Fras1	UTSW	5	96,700,251 (GRCm38)	missense	possibly damaging	0.71
Z1177:Fras1	UTSW	5	96,691,457 (GRCm38)	missense	probably damaging	1.00
Z1177:Fras1	UTSW	5	96,758,142 (GRCm38)	missense	probably benign
Z1177:Fras1	UTSW	5	96,740,983 (GRCm38)	missense	possibly damaging	0.95
Z1177:Fras1	UTSW	5	96,740,811 (GRCm38)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- AGAACTCTGAGAGTGTCTTGCAC -3'
(R):5'- TCTGCTACTGTAGGGACAAGAAG -3'

Sequencing Primer
(F):5'- TGAGAGTGTCTTGCACCTTCTCAC -3'
(R):5'- AATCGCTGTTATCATCACTACCATC -3'

Posted On

2015-10-21