Mutagenetix > Incidental Mutation

Incidental Mutation 'R4697:Tln2'

355784

Institutional Source

Beutler Lab

Gene Symbol

Tln2

Ensembl Gene

ENSMUSG00000052698

Gene Name

talin 2

Synonyms

MMRRC Submission

041947-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.216) question?

Stock #

R4697 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

67217087-67559703 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 67395461 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glutamine at position 76 (R76Q)

Ref Sequence

ENSEMBL: ENSMUSP00000039633 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039662] [ENSMUST00000040025] [ENSMUST00000213584] [ENSMUST00000215784]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000039662
AA Change: R76Q

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000035272
Gene: ENSMUSG00000052698
AA Change: R76Q

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	3.4e-59	PFAM
Pfam:I_LWEQ	661	765	1.9e-10	PFAM
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	2.6e-67	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2384	2531	2.5e-52	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000040025
AA Change: R76Q

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000039633
Gene: ENSMUSG00000052698
AA Change: R76Q

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	8.5e-78	PFAM
low complexity region	674	693	N/A	INTRINSIC
internal_repeat_2	703	763	2.05e-7	PROSPERO
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	9.9e-72	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2383	2533	4.3e-51	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213584
AA Change: R78Q

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215784
AA Change: R78Q

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

Meta Mutation Damage Score

0.2693

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.5%

Validation Efficiency

96% (75/78)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal muscle morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9030624J02Rik	T	A	7: 118,791,448 (GRCm38)	I455N	probably damaging	Het
A2m	T	C	6: 121,638,284 (GRCm38)	M1T	probably null	Het
Aatf	T	A	11: 84,449,138 (GRCm38)	D449V	probably damaging	Het
Acbd3	T	A	1: 180,721,944 (GRCm38)		probably benign	Het
BC005561	A	G	5: 104,522,240 (GRCm38)	K1543E	probably benign	Het
Bicc1	T	A	10: 70,953,484 (GRCm38)	I366F	possibly damaging	Het
Ccdc74a	T	C	16: 17,649,749 (GRCm38)	S184P	possibly damaging	Het
Cntn4	T	C	6: 106,525,485 (GRCm38)	V401A	probably damaging	Het
Cux2	T	C	5: 121,873,753 (GRCm38)	T540A	probably damaging	Het
Disp2	G	T	2: 118,791,684 (GRCm38)	E966*	probably null	Het
Dpp7	A	G	2: 25,354,919 (GRCm38)	Y209H	probably benign	Het
Dstyk	T	A	1: 132,449,487 (GRCm38)	F277Y	probably damaging	Het
Dtx1	A	T	5: 120,694,408 (GRCm38)		probably null	Het
Ear-ps2	G	A	14: 44,047,060 (GRCm38)		noncoding transcript	Het
Ednra	T	C	8: 77,664,995 (GRCm38)	H422R	probably benign	Het
Erlec1	T	A	11: 30,952,640 (GRCm38)	I67F	probably benign	Het
Fam161b	G	A	12: 84,348,558 (GRCm38)		probably benign	Het
Gata5	A	T	2: 180,327,379 (GRCm38)	C345*	probably null	Het
Glmp	T	C	3: 88,328,274 (GRCm38)	V47A	probably damaging	Het
Gm9762	T	A	3: 78,966,550 (GRCm38)		noncoding transcript	Het
Gnas	A	T	2: 174,298,080 (GRCm38)	D14V	probably damaging	Het
Gnl3	T	C	14: 31,017,329 (GRCm38)	S53G	probably damaging	Het
Grhl3	C	T	4: 135,548,466 (GRCm38)	V527M	probably damaging	Het
Hoxd10	T	A	2: 74,694,187 (GRCm38)	L281*	probably null	Het
Kif16b	T	G	2: 142,690,694 (GRCm38)	Y1175S	probably benign	Het
Kif2b	A	G	11: 91,576,846 (GRCm38)	S204P	probably benign	Het
Klhl40	T	A	9: 121,778,734 (GRCm38)	I320N	probably damaging	Het
Ksr2	G	A	5: 117,708,147 (GRCm38)	R693Q	probably damaging	Het
Mis12	A	G	11: 71,025,326 (GRCm38)	K62E	possibly damaging	Het
Mlc1	A	G	15: 88,974,777 (GRCm38)	C102R	probably damaging	Het
Muc5b	T	C	7: 141,857,361 (GRCm38)	I1348T	unknown	Het
Myh7b	A	G	2: 155,629,322 (GRCm38)	E1130G	probably damaging	Het
Nat8f4	T	C	6: 85,901,386 (GRCm38)	T52A	probably benign	Het
Nxpe2	C	A	9: 48,320,521 (GRCm38)	V379L	probably benign	Het
Olfr1231	C	A	2: 89,302,902 (GRCm38)	S230I	possibly damaging	Het
Olfr1231	T	A	2: 89,302,903 (GRCm38)	S230C	probably damaging	Het
Olfr1448	C	T	19: 12,919,934 (GRCm38)	C125Y	probably damaging	Het
Olfr1465	A	T	19: 13,313,717 (GRCm38)	D189E	probably benign	Het
Olfr288	G	A	15: 98,186,868 (GRCm38)	R310W	probably damaging	Het
Pcdhga7	A	T	18: 37,717,208 (GRCm38)	Y756F	probably damaging	Het
Pcsk6	T	A	7: 65,959,241 (GRCm38)	Y284N	probably damaging	Het
Pdcd11	T	C	19: 47,126,347 (GRCm38)	V1367A	possibly damaging	Het
Postn	T	A	3: 54,375,071 (GRCm38)	N484K	probably damaging	Het
Prkd3	C	T	17: 78,961,171 (GRCm38)	V572I	probably benign	Het
Qser1	T	C	2: 104,787,183 (GRCm38)	S1005G	probably benign	Het
Radil	G	T	5: 142,486,801 (GRCm38)	D951E	probably benign	Het
Ripk1	C	T	13: 34,027,942 (GRCm38)	R352*	probably null	Het
Sacs	T	C	14: 61,212,747 (GRCm38)	F4081L	probably benign	Het
Sbf1	A	G	15: 89,315,085 (GRCm38)	V11A	possibly damaging	Het
Sgip1	T	A	4: 102,934,587 (GRCm38)	F536I	probably damaging	Het
Slc45a1	A	G	4: 150,638,284 (GRCm38)	L381P	probably damaging	Het
Smarcad1	C	T	6: 65,052,641 (GRCm38)	P71L	probably benign	Het
Spns1	T	A	7: 126,377,037 (GRCm38)	D14V	probably damaging	Het
Sv2c	T	A	13: 95,986,018 (GRCm38)	I417F	possibly damaging	Het
Tas2r113	T	A	6: 132,893,516 (GRCm38)	M169K	probably benign	Het
Tgm1	A	T	14: 55,705,681 (GRCm38)	N567K	probably benign	Het
Trpm6	T	C	19: 18,853,791 (GRCm38)	V1340A	probably benign	Het
Tspan18	A	T	2: 93,312,030 (GRCm38)		probably null	Het
Txndc11	C	T	16: 11,084,314 (GRCm38)	V679I	probably damaging	Het
Usf1	G	T	1: 171,416,964 (GRCm38)	G144V	possibly damaging	Het
Vmn1r59	T	C	7: 5,454,452 (GRCm38)	Y103C	probably damaging	Het
Vmn2r23	A	T	6: 123,741,826 (GRCm38)	I713F	probably damaging	Het
Vmn2r79	A	T	7: 87,037,960 (GRCm38)	I850F	probably damaging	Het
Wdr90	C	T	17: 25,855,363 (GRCm38)	R676H	probably benign	Het
Zfp867	G	A	11: 59,463,661 (GRCm38)	R281W	probably damaging	Het
Zfp939	T	C	7: 39,472,942 (GRCm38)		noncoding transcript	Het

Other mutations in Tln2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00468:Tln2	APN	9	67,344,187 (GRCm38)	missense	possibly damaging	0.59
IGL01110:Tln2	APN	9	67,250,582 (GRCm38)	nonsense	probably null
IGL01112:Tln2	APN	9	67,311,811 (GRCm38)	missense	probably damaging	1.00
IGL01307:Tln2	APN	9	67,395,467 (GRCm38)	missense	probably benign	0.25
IGL01374:Tln2	APN	9	67,261,923 (GRCm38)	missense	probably damaging	1.00
IGL01625:Tln2	APN	9	67,370,623 (GRCm38)	missense	probably damaging	1.00
IGL01865:Tln2	APN	9	67,250,614 (GRCm38)	nonsense	probably null
IGL01999:Tln2	APN	9	67,392,505 (GRCm38)	missense	possibly damaging	0.81
IGL02002:Tln2	APN	9	67,356,698 (GRCm38)	missense	probably damaging	0.98
IGL02005:Tln2	APN	9	67,392,505 (GRCm38)	missense	possibly damaging	0.81
IGL02015:Tln2	APN	9	67,361,439 (GRCm38)	splice site	probably benign
IGL02368:Tln2	APN	9	67,240,810 (GRCm38)	splice site	probably benign
IGL02444:Tln2	APN	9	67,258,592 (GRCm38)	splice site	probably benign
IGL02646:Tln2	APN	9	67,255,996 (GRCm38)	missense	probably benign	0.43
IGL02744:Tln2	APN	9	67,229,376 (GRCm38)	nonsense	probably null
IGL02869:Tln2	APN	9	67,221,525 (GRCm38)	splice site	probably benign
IGL02930:Tln2	APN	9	67,393,662 (GRCm38)	nonsense	probably null
IGL03100:Tln2	APN	9	67,295,737 (GRCm38)	missense	probably damaging	1.00
IGL03326:Tln2	APN	9	67,334,257 (GRCm38)	missense	possibly damaging	0.67
Harrier	UTSW	9	67,330,552 (GRCm38)	nonsense	probably null
Marsh	UTSW	9	67,272,654 (GRCm38)	missense	probably benign	0.19
BB008:Tln2	UTSW	9	67,258,460 (GRCm38)	critical splice donor site	probably null
BB018:Tln2	UTSW	9	67,258,460 (GRCm38)	critical splice donor site	probably null
R0047:Tln2	UTSW	9	67,240,672 (GRCm38)	splice site	probably benign
R0047:Tln2	UTSW	9	67,240,672 (GRCm38)	splice site	probably benign
R0107:Tln2	UTSW	9	67,370,706 (GRCm38)	missense	probably damaging	1.00
R0494:Tln2	UTSW	9	67,355,197 (GRCm38)	missense	probably benign	0.22
R0884:Tln2	UTSW	9	67,370,733 (GRCm38)	missense	probably damaging	1.00
R0947:Tln2	UTSW	9	67,295,813 (GRCm38)	missense	probably benign	0.08
R0989:Tln2	UTSW	9	67,229,454 (GRCm38)	missense	probably damaging	1.00
R1195:Tln2	UTSW	9	67,258,566 (GRCm38)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,258,566 (GRCm38)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,258,566 (GRCm38)	missense	probably damaging	0.96
R1486:Tln2	UTSW	9	67,311,839 (GRCm38)	missense	probably damaging	1.00
R1527:Tln2	UTSW	9	67,272,668 (GRCm38)	missense	possibly damaging	0.95
R1584:Tln2	UTSW	9	67,296,414 (GRCm38)	missense	probably damaging	1.00
R1636:Tln2	UTSW	9	67,306,532 (GRCm38)	missense	probably damaging	1.00
R1656:Tln2	UTSW	9	67,227,107 (GRCm38)	missense	possibly damaging	0.81
R1707:Tln2	UTSW	9	67,375,807 (GRCm38)	missense	probably benign	0.00
R1749:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1751:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1761:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1767:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1815:Tln2	UTSW	9	67,229,423 (GRCm38)	missense	probably damaging	1.00
R1840:Tln2	UTSW	9	67,342,043 (GRCm38)	missense	probably damaging	1.00
R1847:Tln2	UTSW	9	67,362,687 (GRCm38)	nonsense	probably null
R1964:Tln2	UTSW	9	67,342,135 (GRCm38)	missense	probably benign	0.00
R1968:Tln2	UTSW	9	67,255,901 (GRCm38)	missense	probably damaging	1.00
R2036:Tln2	UTSW	9	67,272,704 (GRCm38)	missense	possibly damaging	0.76
R2038:Tln2	UTSW	9	67,397,653 (GRCm38)	start codon destroyed	probably benign	0.01
R2152:Tln2	UTSW	9	67,302,560 (GRCm38)	missense	probably damaging	1.00
R2153:Tln2	UTSW	9	67,302,560 (GRCm38)	missense	probably damaging	1.00
R2154:Tln2	UTSW	9	67,302,560 (GRCm38)	missense	probably damaging	1.00
R2191:Tln2	UTSW	9	67,355,221 (GRCm38)	missense	probably damaging	1.00
R2192:Tln2	UTSW	9	67,355,221 (GRCm38)	missense	probably damaging	1.00
R2201:Tln2	UTSW	9	67,375,757 (GRCm38)	missense	probably damaging	1.00
R3116:Tln2	UTSW	9	67,355,139 (GRCm38)	missense	probably benign	0.10
R3151:Tln2	UTSW	9	67,330,547 (GRCm38)	critical splice donor site	probably null
R3795:Tln2	UTSW	9	67,255,915 (GRCm38)	missense	probably damaging	0.97
R3953:Tln2	UTSW	9	67,370,629 (GRCm38)	missense	probably damaging	1.00
R4450:Tln2	UTSW	9	67,344,065 (GRCm38)	critical splice donor site	probably null
R4685:Tln2	UTSW	9	67,302,572 (GRCm38)	missense	probably damaging	1.00
R4688:Tln2	UTSW	9	67,397,653 (GRCm38)	start codon destroyed	probably benign	0.01
R4696:Tln2	UTSW	9	67,395,461 (GRCm38)	missense	probably damaging	1.00
R4700:Tln2	UTSW	9	67,346,527 (GRCm38)	missense	probably benign	0.03
R4701:Tln2	UTSW	9	67,346,527 (GRCm38)	missense	probably benign	0.03
R4741:Tln2	UTSW	9	67,386,555 (GRCm38)	critical splice donor site	probably null
R4806:Tln2	UTSW	9	67,331,733 (GRCm38)	missense	probably benign	0.29
R4807:Tln2	UTSW	9	67,331,733 (GRCm38)	missense	probably benign	0.29
R4808:Tln2	UTSW	9	67,331,733 (GRCm38)	missense	probably benign	0.29
R4967:Tln2	UTSW	9	67,355,125 (GRCm38)	missense	probably damaging	0.97
R5061:Tln2	UTSW	9	67,354,468 (GRCm38)	missense	probably benign
R5092:Tln2	UTSW	9	67,256,028 (GRCm38)	missense	probably benign	0.13
R5093:Tln2	UTSW	9	67,334,314 (GRCm38)	missense	probably benign	0.44
R5126:Tln2	UTSW	9	67,258,535 (GRCm38)	missense	probably damaging	1.00
R5204:Tln2	UTSW	9	67,354,482 (GRCm38)	missense	probably benign	0.00
R5236:Tln2	UTSW	9	67,365,923 (GRCm38)	missense	probably damaging	0.99
R5287:Tln2	UTSW	9	67,242,359 (GRCm38)	missense	probably damaging	1.00
R5568:Tln2	UTSW	9	67,311,865 (GRCm38)	missense	probably damaging	1.00
R5571:Tln2	UTSW	9	67,334,320 (GRCm38)	missense	possibly damaging	0.88
R5642:Tln2	UTSW	9	67,296,358 (GRCm38)	missense	probably benign	0.01
R5711:Tln2	UTSW	9	67,392,547 (GRCm38)	missense	probably benign	0.00
R5776:Tln2	UTSW	9	67,258,250 (GRCm38)	missense	probably damaging	1.00
R5791:Tln2	UTSW	9	67,386,605 (GRCm38)	missense	probably damaging	0.98
R5866:Tln2	UTSW	9	67,266,868 (GRCm38)	missense	probably damaging	1.00
R5888:Tln2	UTSW	9	67,229,403 (GRCm38)	missense	probably damaging	1.00
R5902:Tln2	UTSW	9	67,362,717 (GRCm38)	missense	probably benign	0.02
R6106:Tln2	UTSW	9	67,323,020 (GRCm38)	missense	probably damaging	0.99
R6175:Tln2	UTSW	9	67,224,081 (GRCm38)	missense	probably damaging	1.00
R6385:Tln2	UTSW	9	67,278,129 (GRCm38)	missense	probably benign	0.45
R6430:Tln2	UTSW	9	67,272,665 (GRCm38)	missense	probably damaging	1.00
R6441:Tln2	UTSW	9	67,272,689 (GRCm38)	missense	probably damaging	1.00
R6738:Tln2	UTSW	9	67,386,664 (GRCm38)	missense	possibly damaging	0.91
R6776:Tln2	UTSW	9	67,262,905 (GRCm38)	missense	probably damaging	1.00
R6794:Tln2	UTSW	9	67,286,558 (GRCm38)	missense	probably benign	0.07
R6850:Tln2	UTSW	9	67,258,535 (GRCm38)	missense	probably damaging	1.00
R6907:Tln2	UTSW	9	67,397,635 (GRCm38)	missense	probably damaging	0.98
R6909:Tln2	UTSW	9	67,392,532 (GRCm38)	missense	probably damaging	0.97
R6951:Tln2	UTSW	9	67,258,485 (GRCm38)	missense	probably damaging	0.97
R7015:Tln2	UTSW	9	67,362,647 (GRCm38)	missense	possibly damaging	0.55
R7051:Tln2	UTSW	9	67,346,417 (GRCm38)	missense	probably benign	0.00
R7246:Tln2	UTSW	9	67,262,979 (GRCm38)	missense	probably damaging	1.00
R7292:Tln2	UTSW	9	67,346,461 (GRCm38)	missense	probably benign
R7753:Tln2	UTSW	9	67,395,473 (GRCm38)	missense	probably damaging	1.00
R7868:Tln2	UTSW	9	67,348,226 (GRCm38)	missense	probably damaging	1.00
R7931:Tln2	UTSW	9	67,258,460 (GRCm38)	critical splice donor site	probably null
R8023:Tln2	UTSW	9	67,224,064 (GRCm38)	missense	probably damaging	1.00
R8081:Tln2	UTSW	9	67,356,747 (GRCm38)	missense	probably damaging	1.00
R8164:Tln2	UTSW	9	67,319,420 (GRCm38)	missense	probably benign	0.31
R8192:Tln2	UTSW	9	67,346,529 (GRCm38)	nonsense	probably null
R8495:Tln2	UTSW	9	67,354,467 (GRCm38)	missense	probably benign	0.01
R8734:Tln2	UTSW	9	67,272,654 (GRCm38)	missense	probably benign	0.19
R8739:Tln2	UTSW	9	67,258,273 (GRCm38)	missense	probably damaging	1.00
R8757:Tln2	UTSW	9	67,367,218 (GRCm38)	missense	probably damaging	1.00
R8759:Tln2	UTSW	9	67,367,218 (GRCm38)	missense	probably damaging	1.00
R8770:Tln2	UTSW	9	67,323,022 (GRCm38)	missense	probably benign
R8781:Tln2	UTSW	9	67,255,951 (GRCm38)	missense	probably damaging	1.00
R8812:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8814:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8816:Tln2	UTSW	9	67,221,517 (GRCm38)	missense	probably damaging	1.00
R8816:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8833:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8835:Tln2	UTSW	9	67,397,693 (GRCm38)	splice site	probably benign
R8837:Tln2	UTSW	9	67,250,584 (GRCm38)	missense	probably damaging	0.99
R8843:Tln2	UTSW	9	67,395,545 (GRCm38)	missense	probably damaging	1.00
R8864:Tln2	UTSW	9	67,330,552 (GRCm38)	nonsense	probably null
R8867:Tln2	UTSW	9	67,330,550 (GRCm38)	missense	probably damaging	0.98
R8921:Tln2	UTSW	9	67,266,823 (GRCm38)	missense	probably damaging	0.99
R9080:Tln2	UTSW	9	67,346,561 (GRCm38)	missense	probably damaging	1.00
R9083:Tln2	UTSW	9	67,362,645 (GRCm38)	missense	probably damaging	0.96
R9150:Tln2	UTSW	9	67,221,496 (GRCm38)	missense	probably damaging	1.00
R9287:Tln2	UTSW	9	67,370,698 (GRCm38)	missense	probably benign	0.20
R9330:Tln2	UTSW	9	67,321,931 (GRCm38)	missense	possibly damaging	0.61
R9343:Tln2	UTSW	9	67,323,071 (GRCm38)	missense	probably benign	0.10
R9355:Tln2	UTSW	9	67,355,247 (GRCm38)	missense	possibly damaging	0.46
R9383:Tln2	UTSW	9	67,370,761 (GRCm38)	missense	probably benign	0.17
R9386:Tln2	UTSW	9	67,365,967 (GRCm38)	missense	possibly damaging	0.78
R9407:Tln2	UTSW	9	67,229,450 (GRCm38)	missense	probably damaging	1.00
R9483:Tln2	UTSW	9	67,392,487 (GRCm38)	missense	probably damaging	1.00
R9523:Tln2	UTSW	9	67,258,484 (GRCm38)	missense	probably damaging	0.99
R9642:Tln2	UTSW	9	67,250,544 (GRCm38)	missense	probably benign	0.02
R9703:Tln2	UTSW	9	67,386,656 (GRCm38)	missense	probably damaging	1.00
X0027:Tln2	UTSW	9	67,376,853 (GRCm38)	missense	probably damaging	1.00
X0064:Tln2	UTSW	9	67,348,138 (GRCm38)	missense	probably damaging	1.00
X0067:Tln2	UTSW	9	67,370,691 (GRCm38)	missense	probably damaging	1.00
Z1176:Tln2	UTSW	9	67,346,485 (GRCm38)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- TTCCATAGGTAGCTCCCCAG -3'
(R):5'- GTGATGGACAGTATCGTCCTCC -3'

Sequencing Primer
(F):5'- TGGCACCAAGACTGTTTAGC -3'
(R):5'- GATGGACAGTATCGTCCTCCTTTCTG -3'

Posted On

2015-10-21