Incidental Mutation 'R4698:Phf21a'
| ID |
355812 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Phf21a
|
| Ensembl Gene |
ENSMUSG00000058318 |
| Gene Name |
PHD finger protein 21A |
| Synonyms |
PFTF1, Bhc80, Braf35/HDAC complex (Bhc), 80kDa, D030065N23Rik |
| Accession Numbers |
|
| Essential gene? |
Essential
(E-score: 1.000)
|
| Stock # |
R4698 (G1)
|
| Quality Score |
189 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
92093117-92364666 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 92356952 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Aspartic acid to Glycine
at position 445
(D445G)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000106928
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000044036]
[ENSMUST00000068702]
[ENSMUST00000090586]
[ENSMUST00000111290]
[ENSMUST00000111291]
[ENSMUST00000111292]
[ENSMUST00000111293]
[ENSMUST00000111294]
[ENSMUST00000111297]
[ENSMUST00000159961]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000044036
AA Change: D438G
PolyPhen 2
Score 0.952 (Sensitivity: 0.79; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000038497
Gene: ENSMUSG00000058318
AA Change: D438G
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
29 |
62 |
N/A |
INTRINSIC |
|
low complexity region
|
85 |
129 |
N/A |
INTRINSIC |
|
low complexity region
|
165 |
186 |
N/A |
INTRINSIC |
|
AT_hook
|
350 |
362 |
4.28e-1 |
SMART |
|
low complexity region
|
369 |
381 |
N/A |
INTRINSIC |
|
PHD
|
415 |
458 |
3.12e-15 |
SMART |
|
RING
|
416 |
457 |
1.85e-1 |
SMART |
|
coiled coil region
|
482 |
527 |
N/A |
INTRINSIC |
|
low complexity region
|
575 |
595 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000068702
AA Change: D390G
PolyPhen 2
Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)
|
| SMART Domains |
Protein: ENSMUSP00000070649
Gene: ENSMUSG00000058318
AA Change: D390G
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
29 |
62 |
N/A |
INTRINSIC |
|
low complexity region
|
85 |
128 |
N/A |
INTRINSIC |
|
low complexity region
|
164 |
185 |
N/A |
INTRINSIC |
|
PHD
|
367 |
410 |
3.12e-15 |
SMART |
|
RING
|
368 |
409 |
1.85e-1 |
SMART |
|
coiled coil region
|
434 |
479 |
N/A |
INTRINSIC |
|
low complexity region
|
527 |
547 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000090586
AA Change: D522G
PolyPhen 2
Score 0.442 (Sensitivity: 0.89; Specificity: 0.90)
|
| SMART Domains |
Protein: ENSMUSP00000088074
Gene: ENSMUSG00000058318
AA Change: D522G
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
29 |
62 |
N/A |
INTRINSIC |
|
low complexity region
|
85 |
128 |
N/A |
INTRINSIC |
|
low complexity region
|
249 |
270 |
N/A |
INTRINSIC |
|
AT_hook
|
434 |
446 |
4.28e-1 |
SMART |
|
low complexity region
|
453 |
465 |
N/A |
INTRINSIC |
|
PHD
|
499 |
542 |
3.12e-15 |
SMART |
|
RING
|
500 |
541 |
1.85e-1 |
SMART |
|
coiled coil region
|
566 |
611 |
N/A |
INTRINSIC |
|
low complexity region
|
659 |
679 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000111290
AA Change: D493G
PolyPhen 2
Score 0.174 (Sensitivity: 0.92; Specificity: 0.87)
|
| SMART Domains |
Protein: ENSMUSP00000106921
Gene: ENSMUSG00000058318
AA Change: D493G
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
29 |
62 |
N/A |
INTRINSIC |
|
low complexity region
|
85 |
128 |
N/A |
INTRINSIC |
|
low complexity region
|
249 |
270 |
N/A |
INTRINSIC |
|
AT_hook
|
405 |
417 |
4.28e-1 |
SMART |
|
low complexity region
|
424 |
436 |
N/A |
INTRINSIC |
|
PHD
|
470 |
513 |
3.12e-15 |
SMART |
|
RING
|
471 |
512 |
1.85e-1 |
SMART |
|
coiled coil region
|
537 |
582 |
N/A |
INTRINSIC |
|
low complexity region
|
630 |
650 |
N/A |
INTRINSIC |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000111291
AA Change: D438G
PolyPhen 2
Score 0.952 (Sensitivity: 0.79; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000106922
Gene: ENSMUSG00000058318
AA Change: D438G
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
29 |
62 |
N/A |
INTRINSIC |
|
low complexity region
|
85 |
129 |
N/A |
INTRINSIC |
|
low complexity region
|
165 |
186 |
N/A |
INTRINSIC |
|
AT_hook
|
350 |
362 |
4.28e-1 |
SMART |
|
low complexity region
|
369 |
381 |
N/A |
INTRINSIC |
|
PHD
|
415 |
458 |
3.12e-15 |
SMART |
|
RING
|
416 |
457 |
1.85e-1 |
SMART |
|
coiled coil region
|
482 |
527 |
N/A |
INTRINSIC |
|
low complexity region
|
575 |
595 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000111292
AA Change: D390G
PolyPhen 2
Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)
|
| SMART Domains |
Protein: ENSMUSP00000106923
Gene: ENSMUSG00000058318
AA Change: D390G
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
29 |
62 |
N/A |
INTRINSIC |
|
low complexity region
|
85 |
128 |
N/A |
INTRINSIC |
|
low complexity region
|
164 |
185 |
N/A |
INTRINSIC |
|
PHD
|
367 |
410 |
3.12e-15 |
SMART |
|
RING
|
368 |
409 |
1.85e-1 |
SMART |
|
coiled coil region
|
434 |
479 |
N/A |
INTRINSIC |
|
low complexity region
|
527 |
547 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000111293
AA Change: D522G
PolyPhen 2
Score 0.442 (Sensitivity: 0.89; Specificity: 0.90)
|
| SMART Domains |
Protein: ENSMUSP00000106924
Gene: ENSMUSG00000058318
AA Change: D522G
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
29 |
62 |
N/A |
INTRINSIC |
|
low complexity region
|
85 |
128 |
N/A |
INTRINSIC |
|
low complexity region
|
249 |
270 |
N/A |
INTRINSIC |
|
AT_hook
|
434 |
446 |
4.28e-1 |
SMART |
|
low complexity region
|
453 |
465 |
N/A |
INTRINSIC |
|
PHD
|
499 |
542 |
3.12e-15 |
SMART |
|
RING
|
500 |
541 |
1.85e-1 |
SMART |
|
coiled coil region
|
566 |
611 |
N/A |
INTRINSIC |
|
low complexity region
|
659 |
679 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000111294
AA Change: D475G
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000106925
Gene: ENSMUSG00000058318
AA Change: D475G
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
29 |
62 |
N/A |
INTRINSIC |
|
low complexity region
|
85 |
128 |
N/A |
INTRINSIC |
|
low complexity region
|
249 |
270 |
N/A |
INTRINSIC |
|
PHD
|
452 |
495 |
3.12e-15 |
SMART |
|
RING
|
453 |
494 |
1.85e-1 |
SMART |
|
coiled coil region
|
519 |
564 |
N/A |
INTRINSIC |
|
low complexity region
|
612 |
632 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000111297
AA Change: D445G
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000106928
Gene: ENSMUSG00000058318
AA Change: D445G
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
29 |
62 |
N/A |
INTRINSIC |
|
low complexity region
|
85 |
129 |
N/A |
INTRINSIC |
|
low complexity region
|
248 |
269 |
N/A |
INTRINSIC |
|
PHD
|
422 |
465 |
3.12e-15 |
SMART |
|
RING
|
423 |
464 |
1.85e-1 |
SMART |
|
coiled coil region
|
489 |
534 |
N/A |
INTRINSIC |
|
low complexity region
|
582 |
602 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000159961
AA Change: D409G
PolyPhen 2
Score 0.151 (Sensitivity: 0.92; Specificity: 0.87)
|
| SMART Domains |
Protein: ENSMUSP00000123955
Gene: ENSMUSG00000058318
AA Change: D409G
| Domain | Start | End | E-Value | Type |
|---|
|
coiled coil region
|
1 |
34 |
N/A |
INTRINSIC |
|
low complexity region
|
57 |
100 |
N/A |
INTRINSIC |
|
low complexity region
|
136 |
157 |
N/A |
INTRINSIC |
|
AT_hook
|
321 |
333 |
4.28e-1 |
SMART |
|
low complexity region
|
340 |
352 |
N/A |
INTRINSIC |
|
PHD
|
386 |
429 |
3.12e-15 |
SMART |
|
RING
|
387 |
428 |
1.85e-1 |
SMART |
|
coiled coil region
|
453 |
498 |
N/A |
INTRINSIC |
|
low complexity region
|
546 |
566 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000161044
|
| Predicted Effect |
unknown
Transcript: ENSMUST00000161067
AA Change: D162G
|
| SMART Domains |
Protein: ENSMUSP00000124255
Gene: ENSMUSG00000058318
AA Change: D162G
| Domain | Start | End | E-Value | Type |
|---|
|
PHD
|
140 |
183 |
3.12e-15 |
SMART |
|
RING
|
141 |
182 |
1.85e-1 |
SMART |
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 96.9%
- 20x: 94.3%
|
| Validation Efficiency |
|
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]).[supplied by OMIM, Nov 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit neonatal lethality and insufficient milk-sucking behavior. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 60 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1700122O11Rik |
A |
G |
17: 48,038,240 (GRCm38) |
V11A |
possibly damaging |
Het |
| 6030452D12Rik |
T |
C |
8: 106,504,347 (GRCm38) |
I55T |
unknown |
Het |
| Aadacl2 |
A |
G |
3: 60,025,039 (GRCm38) |
D325G |
probably benign |
Het |
| Adh1 |
A |
G |
3: 138,282,513 (GRCm38) |
S113G |
probably benign |
Het |
| Agxt2 |
T |
C |
15: 10,392,044 (GRCm38) |
|
probably null |
Het |
| Aicda |
C |
T |
6: 122,553,888 (GRCm38) |
|
probably benign |
Het |
| Aifm2 |
T |
C |
10: 61,727,756 (GRCm38) |
M135T |
probably benign |
Het |
| Asah2 |
T |
C |
19: 32,054,471 (GRCm38) |
|
probably null |
Het |
| Btbd17 |
T |
C |
11: 114,791,717 (GRCm38) |
R390G |
probably damaging |
Het |
| Cadps |
T |
C |
14: 12,705,654 (GRCm38) |
E247G |
possibly damaging |
Het |
| Cds2 |
T |
C |
2: 132,304,953 (GRCm38) |
I383T |
probably damaging |
Het |
| Celf3 |
T |
A |
3: 94,484,867 (GRCm38) |
|
probably null |
Het |
| Crhr2 |
T |
C |
6: 55,102,867 (GRCm38) |
S162G |
possibly damaging |
Het |
| Dapl1 |
A |
T |
2: 59,504,774 (GRCm38) |
K91* |
probably null |
Het |
| Ddx60 |
A |
C |
8: 62,012,424 (GRCm38) |
M1372L |
probably benign |
Het |
| Dnah2 |
A |
T |
11: 69,498,532 (GRCm38) |
F845I |
probably damaging |
Het |
| Dscam |
T |
C |
16: 96,610,324 (GRCm38) |
D1784G |
probably damaging |
Het |
| Ech1 |
T |
C |
7: 28,832,053 (GRCm38) |
V310A |
probably benign |
Het |
| Eef1g |
A |
G |
19: 8,977,966 (GRCm38) |
D393G |
possibly damaging |
Het |
| Eif3k |
T |
C |
7: 28,972,544 (GRCm38) |
I172V |
possibly damaging |
Het |
| Foxd4 |
A |
G |
19: 24,900,261 (GRCm38) |
F192L |
probably damaging |
Het |
| Gpr153 |
T |
A |
4: 152,281,783 (GRCm38) |
S268R |
probably damaging |
Het |
| Hist2h3b |
C |
A |
3: 96,269,078 (GRCm38) |
R129S |
probably damaging |
Het |
| Ivl |
T |
C |
3: 92,571,391 (GRCm38) |
K456E |
unknown |
Het |
| Kif20b |
A |
T |
19: 34,951,544 (GRCm38) |
D1190V |
probably damaging |
Het |
| Kif21a |
T |
A |
15: 90,956,305 (GRCm38) |
I1223L |
possibly damaging |
Het |
| Lmf1 |
T |
C |
17: 25,579,350 (GRCm38) |
V55A |
probably damaging |
Het |
| Loxhd1 |
A |
G |
18: 77,372,291 (GRCm38) |
E659G |
possibly damaging |
Het |
| Lrrc37a |
T |
C |
11: 103,504,104 (GRCm38) |
E165G |
possibly damaging |
Het |
| Mios |
T |
C |
6: 8,228,113 (GRCm38) |
Y677H |
possibly damaging |
Het |
| Mnx1 |
T |
A |
5: 29,474,059 (GRCm38) |
K342M |
unknown |
Het |
| Mtpap |
A |
T |
18: 4,375,724 (GRCm38) |
T35S |
possibly damaging |
Het |
| Ndc1 |
C |
G |
4: 107,411,137 (GRCm38) |
D623E |
probably benign |
Het |
| Nedd4l |
A |
G |
18: 65,203,880 (GRCm38) |
Y666C |
probably damaging |
Het |
| Olfr1022 |
A |
G |
2: 85,869,252 (GRCm38) |
Y220C |
possibly damaging |
Het |
| Olfr1445 |
T |
G |
19: 12,884,621 (GRCm38) |
F247V |
probably benign |
Het |
| Olfr204 |
T |
C |
16: 59,315,357 (GRCm38) |
T17A |
probably damaging |
Het |
| Olfr605 |
T |
A |
7: 103,442,635 (GRCm38) |
I163F |
possibly damaging |
Het |
| Papss1 |
G |
T |
3: 131,607,331 (GRCm38) |
V369F |
probably damaging |
Het |
| Pcdhac2 |
T |
A |
18: 37,145,769 (GRCm38) |
Y601N |
probably damaging |
Het |
| Pde7a |
C |
T |
3: 19,310,931 (GRCm38) |
R24Q |
probably damaging |
Het |
| Plekho1 |
T |
A |
3: 95,995,652 (GRCm38) |
N15I |
possibly damaging |
Het |
| Pprc1 |
T |
C |
19: 46,069,195 (GRCm38) |
|
probably benign |
Het |
| Ralgapa1 |
A |
T |
12: 55,677,276 (GRCm38) |
|
probably null |
Het |
| Rrp12 |
T |
C |
19: 41,873,042 (GRCm38) |
E942G |
probably benign |
Het |
| Skor1 |
T |
C |
9: 63,144,548 (GRCm38) |
D685G |
probably benign |
Het |
| Smim23 |
A |
T |
11: 32,824,510 (GRCm38) |
I3N |
possibly damaging |
Het |
| Spata7 |
A |
T |
12: 98,664,277 (GRCm38) |
I333F |
probably damaging |
Het |
| Sppl2c |
T |
C |
11: 104,188,315 (GRCm38) |
I647T |
probably benign |
Het |
| Srgap1 |
C |
A |
10: 121,792,487 (GRCm38) |
R837L |
probably benign |
Het |
| Stpg2 |
C |
A |
3: 139,309,229 (GRCm38) |
P385H |
probably damaging |
Het |
| Tmem238 |
C |
A |
7: 4,789,017 (GRCm38) |
E19* |
probably null |
Het |
| Top2b |
A |
T |
14: 16,387,331 (GRCm38) |
K140* |
probably null |
Het |
| Trp53 |
T |
A |
11: 69,588,422 (GRCm38) |
L142* |
probably null |
Het |
| Tyro3 |
A |
T |
2: 119,803,270 (GRCm38) |
D133V |
probably damaging |
Het |
| Virma |
T |
A |
4: 11,528,636 (GRCm38) |
I1291N |
probably damaging |
Het |
| Vmn1r213 |
A |
G |
13: 23,011,337 (GRCm38) |
|
probably benign |
Het |
| Zbtb10 |
T |
C |
3: 9,264,550 (GRCm38) |
S323P |
possibly damaging |
Het |
| Zfp521 |
A |
T |
18: 13,845,603 (GRCm38) |
N584K |
probably damaging |
Het |
| Zfp944 |
A |
G |
17: 22,339,199 (GRCm38) |
C356R |
probably damaging |
Het |
|
| Other mutations in Phf21a |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00331:Phf21a
|
APN |
2 |
92,348,029 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL00826:Phf21a
|
APN |
2 |
92,344,536 (GRCm38) |
splice site |
probably benign |
|
|
IGL01859:Phf21a
|
APN |
2 |
92,328,356 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02124:Phf21a
|
APN |
2 |
92,349,422 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02724:Phf21a
|
APN |
2 |
92,360,247 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL03155:Phf21a
|
APN |
2 |
92,320,266 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0308:Phf21a
|
UTSW |
2 |
92,330,777 (GRCm38) |
missense |
possibly damaging |
0.86 |
|
R1251:Phf21a
|
UTSW |
2 |
92,359,199 (GRCm38) |
missense |
probably benign |
0.00 |
|
R1739:Phf21a
|
UTSW |
2 |
92,360,299 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
R1775:Phf21a
|
UTSW |
2 |
92,330,515 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2013:Phf21a
|
UTSW |
2 |
92,228,483 (GRCm38) |
critical splice donor site |
probably null |
|
|
R2064:Phf21a
|
UTSW |
2 |
92,327,077 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R2073:Phf21a
|
UTSW |
2 |
92,348,036 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4901:Phf21a
|
UTSW |
2 |
92,357,001 (GRCm38) |
nonsense |
probably null |
|
|
R5055:Phf21a
|
UTSW |
2 |
92,351,856 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5249:Phf21a
|
UTSW |
2 |
92,228,477 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5401:Phf21a
|
UTSW |
2 |
92,351,752 (GRCm38) |
missense |
possibly damaging |
0.71 |
|
R5770:Phf21a
|
UTSW |
2 |
92,351,854 (GRCm38) |
missense |
possibly damaging |
0.52 |
|
R5969:Phf21a
|
UTSW |
2 |
92,221,611 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R6008:Phf21a
|
UTSW |
2 |
92,351,752 (GRCm38) |
missense |
possibly damaging |
0.71 |
|
R6012:Phf21a
|
UTSW |
2 |
92,351,775 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6128:Phf21a
|
UTSW |
2 |
92,351,608 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R6354:Phf21a
|
UTSW |
2 |
92,348,937 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7075:Phf21a
|
UTSW |
2 |
92,360,379 (GRCm38) |
nonsense |
probably null |
|
|
R7117:Phf21a
|
UTSW |
2 |
92,359,157 (GRCm38) |
missense |
probably benign |
0.25 |
|
R7270:Phf21a
|
UTSW |
2 |
92,327,139 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R7603:Phf21a
|
UTSW |
2 |
92,357,007 (GRCm38) |
missense |
probably benign |
0.08 |
|
R7708:Phf21a
|
UTSW |
2 |
92,327,166 (GRCm38) |
critical splice donor site |
probably null |
|
|
R7946:Phf21a
|
UTSW |
2 |
92,359,167 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R9788:Phf21a
|
UTSW |
2 |
92,351,633 (GRCm38) |
critical splice donor site |
probably null |
|
|
Z1177:Phf21a
|
UTSW |
2 |
92,230,714 (GRCm38) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- AAAATTGGAGGCCCATCTCTGAG -3'
(R):5'- TTCATGAACCAGGAGGAGAACC -3'
Sequencing Primer
(F):5'- AGGCCCATCTCTGAGCCATC -3'
(R):5'- CATGAACCAGGAGGAGAACCAAGAG -3'
|
| Posted On |
2015-10-21 |
Incidental Mutation