Mutagenetix > Incidental Mutation

Incidental Mutation 'R0402:Or4k35'

35597

Institutional Source

Beutler Lab

Gene Symbol

Or4k35

Ensembl Gene

ENSMUSG00000074965

Gene Name

olfactory receptor family 4 subfamily K member 35

Synonyms

MOR248-11, Olfr1277, GA_x6K02T2Q125-72321818-72320907

MMRRC Submission

038607-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R0402 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

111099799-111100710 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 111100208 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 168 (F168S)

Ref Sequence

ENSEMBL: ENSMUSP00000150703 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099619] [ENSMUST00000214760]

AlphaFold

Q8VF41

Predicted Effect

probably damaging
Transcript: ENSMUST00000099619
AA Change: F168S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000097214
Gene: ENSMUSG00000074965
AA Change: F168S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	303	5.5e-50	PFAM
Pfam:7tm_1	41	287	7.2e-20	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000214760
AA Change: F168S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 98.2%
3x: 97.0%
10x: 94.5%
20x: 88.7%

Validation Efficiency

100% (74/74)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017N19Rik	A	T	10: 100,445,115 (GRCm39)	K165N	probably damaging	Het
Adam6b	T	A	12: 113,453,615 (GRCm39)	M144K	probably damaging	Het
Arhgap44	T	C	11: 64,922,903 (GRCm39)		probably benign	Het
Arl5c	T	A	11: 97,885,939 (GRCm39)	I21F	probably damaging	Het
Bglap2	C	T	3: 88,285,552 (GRCm39)	G40D	probably damaging	Het
Bptf	T	C	11: 106,964,940 (GRCm39)	E1303G	probably damaging	Het
Calhm1	T	C	19: 47,129,896 (GRCm39)	T209A	probably damaging	Het
Ccr8	A	G	9: 119,923,976 (GRCm39)		probably null	Het
Chkb	C	T	15: 89,313,610 (GRCm39)	R65Q	probably benign	Het
Col4a1	T	C	8: 11,249,838 (GRCm39)		probably benign	Het
Cryzl2	A	G	1: 157,292,014 (GRCm39)	T98A	probably benign	Het
D430041D05Rik	T	C	2: 103,998,509 (GRCm39)	T1080A	probably damaging	Het
Dhx8	C	A	11: 101,643,223 (GRCm39)	T765N	probably damaging	Het
Dicer1	T	C	12: 104,697,323 (GRCm39)	D78G	probably benign	Het
Drd2	A	G	9: 49,316,271 (GRCm39)	I344V	probably benign	Het
Edil3	A	T	13: 89,347,570 (GRCm39)		probably benign	Het
Fbxw19	C	T	9: 109,313,493 (GRCm39)	G235D	probably benign	Het
Fzd1	T	C	5: 4,805,702 (GRCm39)	K627E	possibly damaging	Het
Garin1b	A	G	6: 29,323,901 (GRCm39)	T209A	probably benign	Het
Garin4	T	C	1: 190,896,637 (GRCm39)	D2G	probably benign	Het
Gm10638	A	G	8: 87,472,828 (GRCm39)		probably benign	Het
H6pd	G	T	4: 150,080,773 (GRCm39)	A24E	probably damaging	Het
Hectd2	G	T	19: 36,578,929 (GRCm39)		probably null	Het
Hps5	A	G	7: 46,440,333 (GRCm39)		probably benign	Het
Irx3	T	C	8: 92,527,296 (GRCm39)	N136S	possibly damaging	Het
Kcmf1	T	C	6: 72,826,568 (GRCm39)	M1V	probably null	Het
Klrb1	A	T	6: 128,687,583 (GRCm39)	F104I	probably benign	Het
Lrfn5	T	C	12: 61,886,803 (GRCm39)	M197T	probably benign	Het
Mpdz	A	C	4: 81,279,677 (GRCm39)	M51R	possibly damaging	Het
Mtbp	G	T	15: 55,432,466 (GRCm39)	E258*	probably null	Het
Mylk3	T	A	8: 86,079,539 (GRCm39)	H373L	probably damaging	Het
Myrfl	A	G	10: 116,664,882 (GRCm39)	S383P	probably damaging	Het
Nt5c	T	C	11: 115,381,468 (GRCm39)	*195W	probably null	Het
Ocstamp	A	G	2: 165,238,184 (GRCm39)	V360A	possibly damaging	Het
Or10ak16	A	T	4: 118,750,426 (GRCm39)	I49F	possibly damaging	Het
Or10al6	T	A	17: 38,083,284 (GRCm39)	C247S	probably damaging	Het
Or4p19	A	T	2: 88,242,378 (GRCm39)	V208D	probably damaging	Het
Or5an9	A	G	19: 12,186,953 (GRCm39)	T8A	probably damaging	Het
Otop2	T	C	11: 115,217,234 (GRCm39)		probably benign	Het
Pom121l2	A	T	13: 22,172,649 (GRCm39)		probably benign	Het
Pon2	T	A	6: 5,272,410 (GRCm39)	K137*	probably null	Het
Ppip5k2	T	A	1: 97,647,579 (GRCm39)	Q1049L	probably benign	Het
Ralgapa2	C	T	2: 146,276,729 (GRCm39)	V504M	probably damaging	Het
Rph3a	G	A	5: 121,080,317 (GRCm39)	H654Y	probably damaging	Het
Sh2d1b1	T	C	1: 170,107,342 (GRCm39)		probably benign	Het
Slc15a2	G	A	16: 36,595,960 (GRCm39)	T154I	probably benign	Het
Slc45a3	T	C	1: 131,905,265 (GRCm39)	V96A	possibly damaging	Het
Slc7a4	A	G	16: 17,393,497 (GRCm39)	S101P	probably damaging	Het
Smco2	T	C	6: 146,772,633 (GRCm39)		probably benign	Het
Spata2	A	T	2: 167,325,580 (GRCm39)	V413E	probably benign	Het
Specc1l	A	G	10: 75,082,260 (GRCm39)	E552G	probably damaging	Het
Sstr5	C	T	17: 25,711,008 (GRCm39)	V74M	probably benign	Het
Timm50	G	A	7: 28,006,280 (GRCm39)	R274W	probably damaging	Het
Tll2	A	G	19: 41,087,132 (GRCm39)	V573A	possibly damaging	Het
Tm7sf3	C	A	6: 146,507,685 (GRCm39)	R459M	possibly damaging	Het
Txk	A	G	5: 72,889,105 (GRCm39)		probably null	Het
Uroc1	A	G	6: 90,324,284 (GRCm39)	D436G	probably damaging	Het
Vmn1r13	T	A	6: 57,187,083 (GRCm39)	Y81N	possibly damaging	Het
Vmn2r19	A	G	6: 123,313,141 (GRCm39)	E737G	probably damaging	Het
Wfs1	A	G	5: 37,134,324 (GRCm39)		probably benign	Het
Zfp1	G	A	8: 112,396,875 (GRCm39)	E285K	probably damaging	Het
Zfp1005	T	A	2: 150,111,136 (GRCm39)	C609S	possibly damaging	Het
Zfp661	G	A	2: 127,419,640 (GRCm39)	Q167*	probably null	Het
Zswim8	T	C	14: 20,760,834 (GRCm39)	F36S	probably damaging	Het
Zw10	A	G	9: 48,980,023 (GRCm39)	T385A	probably benign	Het

Other mutations in Or4k35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01552:Or4k35	APN	2	111,100,257 (GRCm39)	missense	probably damaging	1.00
IGL02550:Or4k35	APN	2	111,100,349 (GRCm39)	missense	probably damaging	0.99
IGL02661:Or4k35	APN	2	111,099,811 (GRCm39)	missense	possibly damaging	0.56
IGL02940:Or4k35	APN	2	111,100,073 (GRCm39)	missense	probably damaging	0.99
R0121:Or4k35	UTSW	2	111,100,659 (GRCm39)	missense	probably benign	0.01
R0903:Or4k35	UTSW	2	111,100,701 (GRCm39)	missense	probably benign
R1804:Or4k35	UTSW	2	111,100,275 (GRCm39)	missense	probably benign
R1806:Or4k35	UTSW	2	111,100,622 (GRCm39)	missense	possibly damaging	0.65
R1965:Or4k35	UTSW	2	111,099,938 (GRCm39)	missense	probably damaging	1.00
R3718:Or4k35	UTSW	2	111,100,571 (GRCm39)	missense	probably benign
R4044:Or4k35	UTSW	2	111,099,927 (GRCm39)	missense	probably benign	0.01
R4401:Or4k35	UTSW	2	111,100,178 (GRCm39)	missense	probably damaging	1.00
R4518:Or4k35	UTSW	2	111,100,263 (GRCm39)	missense	probably benign
R5309:Or4k35	UTSW	2	111,100,655 (GRCm39)	missense	probably benign	0.00
R5312:Or4k35	UTSW	2	111,100,655 (GRCm39)	missense	probably benign	0.00
R5367:Or4k35	UTSW	2	111,100,235 (GRCm39)	missense	possibly damaging	0.81
R5727:Or4k35	UTSW	2	111,100,197 (GRCm39)	nonsense	probably null
R5772:Or4k35	UTSW	2	111,100,057 (GRCm39)	nonsense	probably null
R5827:Or4k35	UTSW	2	111,100,266 (GRCm39)	missense	probably damaging	1.00
R5882:Or4k35	UTSW	2	111,100,484 (GRCm39)	missense	probably damaging	0.99
R6036:Or4k35	UTSW	2	111,099,957 (GRCm39)	missense	probably damaging	1.00
R6036:Or4k35	UTSW	2	111,099,957 (GRCm39)	missense	probably damaging	1.00
R6728:Or4k35	UTSW	2	111,100,018 (GRCm39)	missense	probably benign	0.00
R7629:Or4k35	UTSW	2	111,100,221 (GRCm39)	missense	probably benign	0.00
R8377:Or4k35	UTSW	2	111,099,983 (GRCm39)	missense	probably damaging	1.00
R8870:Or4k35	UTSW	2	111,100,464 (GRCm39)	missense	possibly damaging	0.61
R9100:Or4k35	UTSW	2	111,100,094 (GRCm39)	missense	probably benign	0.35
R9668:Or4k35	UTSW	2	111,100,287 (GRCm39)	missense	probably benign	0.03
R9690:Or4k35	UTSW	2	111,099,822 (GRCm39)	missense	probably damaging	0.98
R9715:Or4k35	UTSW	2	111,100,623 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGTCCACCCAGGTTATGCTGACTG -3'
(R):5'- ACTGCTTGTATCAATGGCTTATGACCG -3'

Sequencing Primer
(F):5'- TAATACCACCACTGTGATGTGGG -3'
(R):5'- GATGGCGTCATGGATGATTG -3'

Posted On

2013-05-09