Incidental Mutation 'R4702:Pla2g15'
ID356139
Institutional Source Beutler Lab
Gene Symbol Pla2g15
Ensembl Gene ENSMUSG00000031903
Gene Namephospholipase A2, group XV
SynonymsLpla2, LLPL, Lypla3, C87498
MMRRC Submission 041950-MU
Accession Numbers
Is this an essential gene? Possibly non essential (E-score: 0.262) question?
Stock #R4702 (G1)
Quality Score225
Status Not validated
Chromosome8
Chromosomal Location106150399-106164715 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 106163059 bp
ZygosityHeterozygous
Amino Acid Change Methionine to Lysine at position 321 (M321K)
Ref Sequence ENSEMBL: ENSMUSP00000034377 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000034377] [ENSMUST00000212963]
Predicted Effect probably benign
Transcript: ENSMUST00000034377
AA Change: M321K

PolyPhen 2 Score 0.084 (Sensitivity: 0.93; Specificity: 0.85)
SMART Domains Protein: ENSMUSP00000034377
Gene: ENSMUSG00000031903
AA Change: M321K

DomainStartEndE-ValueType
signal peptide 1 33 N/A INTRINSIC
Pfam:LCAT 72 399 6.2e-84 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000212059
Predicted Effect noncoding transcript
Transcript: ENSMUST00000212116
Predicted Effect probably benign
Transcript: ENSMUST00000212963
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.1%
  • 20x: 95.1%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice do not develop atherosclerotic lesions even when fed an atherogenic diet. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 75 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2810474O19Rik A G 6: 149,329,403 T1316A probably benign Het
4921509C19Rik T C 2: 151,472,589 T390A probably benign Het
Adam25 G T 8: 40,754,126 C143F probably damaging Het
Adra1a T C 14: 66,637,559 probably benign Het
Aff1 T C 5: 103,811,069 Y343H probably damaging Het
Antxr2 C T 5: 97,949,169 probably null Het
Ap1m2 A G 9: 21,298,295 F362L probably benign Het
Apobec4 A G 1: 152,756,250 T10A probably benign Het
Armc3 A G 2: 19,309,981 N822S probably damaging Het
Atr A C 9: 95,920,355 T1767P possibly damaging Het
B4galnt1 G T 10: 127,167,525 V172F possibly damaging Het
Bloc1s1 T A 10: 128,923,398 Q13L probably damaging Het
Blvra A C 2: 127,092,062 I125L probably benign Het
Caps2 T C 10: 112,208,347 F484L probably damaging Het
Cep76 A T 18: 67,634,898 I188K possibly damaging Het
Cidea T A 18: 67,367,428 F187I probably benign Het
Cntn3 T A 6: 102,165,331 N1025I probably benign Het
Cntnap3 A T 13: 64,778,862 C565S probably benign Het
Cyp2d22 A C 15: 82,375,917 L22R probably damaging Het
Cyp2d26 C T 15: 82,792,447 probably benign Het
Cyp2j12 A T 4: 96,132,993 probably null Het
Dnajb13 T C 7: 100,504,541 N229S probably benign Het
Dpys A G 15: 39,793,402 V423A possibly damaging Het
Eif4e1b A T 13: 54,787,325 I222F probably damaging Het
Eif5b A T 1: 38,018,877 N87Y unknown Het
Enam T A 5: 88,503,791 L1053* probably null Het
Epha7 T A 4: 28,961,425 L890Q probably damaging Het
Fancm T A 12: 65,122,052 S1730T possibly damaging Het
Flrt3 A G 2: 140,661,655 F18L probably benign Het
Git2 A G 5: 114,745,482 S396P probably damaging Het
H2-M10.4 A G 17: 36,461,982 I36T probably benign Het
Igfn1 G A 1: 135,967,209 S1873L possibly damaging Het
Ints12 A T 3: 133,096,785 D10V probably damaging Het
Kbtbd2 A G 6: 56,779,303 S483P probably benign Het
Kcnv2 G C 19: 27,323,567 A273P probably damaging Het
Klc3 T G 7: 19,395,831 D371A probably damaging Het
Lama3 T A 18: 12,578,029 L1567* probably null Het
Ldhal6b G T 17: 5,418,307 H117Q probably damaging Het
Lrriq1 G T 10: 103,215,749 Q381K possibly damaging Het
Mrps31 T A 8: 22,419,738 L140Q probably damaging Het
Myo15b A G 11: 115,884,008 T2119A probably benign Het
Nkx6-2 T C 7: 139,581,540 D243G probably damaging Het
Olfr374 T A 8: 72,110,200 F211L probably damaging Het
Olfr918 A T 9: 38,673,480 M1K probably null Het
Papln C T 12: 83,781,983 T821I probably benign Het
Pitpnb T G 5: 111,371,352 V166G probably benign Het
Plcxd3 C A 15: 4,375,787 S25R probably benign Het
Ppargc1a T C 5: 51,495,696 I175V possibly damaging Het
Ppp1r13b T A 12: 111,833,281 Q687H probably benign Het
Prpf3 A G 3: 95,834,092 V584A probably damaging Het
Psen2 A G 1: 180,227,724 L399S probably damaging Het
Ptchd3 G A 11: 121,836,409 V370I probably damaging Het
Rasa3 G T 8: 13,570,394 D758E probably benign Het
Reck T C 4: 43,898,060 C113R probably damaging Het
Ric1 T A 19: 29,598,017 F1009I possibly damaging Het
Rrp12 T C 19: 41,871,536 N1035S probably damaging Het
Rtel1 T A 2: 181,352,169 S849T probably benign Het
Scn10a A T 9: 119,633,791 Y1060N possibly damaging Het
Selenov A G 7: 28,288,011 L314P probably damaging Het
Selplg T C 5: 113,819,033 D404G probably benign Het
Slc7a14 T A 3: 31,230,398 Y263F probably damaging Het
Slco3a1 A G 7: 74,320,567 S431P probably damaging Het
Speer3 C G 5: 13,796,380 A238G possibly damaging Het
Spopl A T 2: 23,515,297 probably null Het
Stk10 A T 11: 32,555,172 T69S probably benign Het
Tbxas1 T C 6: 39,083,857 probably null Het
Tec G A 5: 72,783,731 P161L possibly damaging Het
Tnip1 A G 11: 54,924,402 S339P probably benign Het
Tsen34 T A 7: 3,700,633 V290D probably damaging Het
Tuba1a T A 15: 98,951,682 I5F possibly damaging Het
Vmn1r31 C A 6: 58,471,968 *304L probably null Het
Vmn1r63 T C 7: 5,803,517 R39G possibly damaging Het
Xylt2 A G 11: 94,669,529 Y307H possibly damaging Het
Zfp65 A G 13: 67,724,222 M1T probably null Het
Zmym4 T C 4: 126,923,165 I247V probably benign Het
Other mutations in Pla2g15
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01522:Pla2g15 APN 8 106163116 missense probably benign 0.00
IGL02719:Pla2g15 APN 8 106160196 missense probably benign 0.01
IGL03065:Pla2g15 APN 8 106160219 missense probably benign 0.20
R0125:Pla2g15 UTSW 8 106163124 missense probably benign 0.00
R1691:Pla2g15 UTSW 8 106154949 missense possibly damaging 0.93
R1939:Pla2g15 UTSW 8 106163295 missense probably damaging 0.96
R3887:Pla2g15 UTSW 8 106161135 missense probably damaging 0.99
R4633:Pla2g15 UTSW 8 106160255 missense probably damaging 1.00
R4703:Pla2g15 UTSW 8 106163059 missense probably benign 0.08
R4705:Pla2g15 UTSW 8 106163059 missense probably benign 0.08
R4766:Pla2g15 UTSW 8 106163071 missense probably damaging 1.00
R4928:Pla2g15 UTSW 8 106163218 nonsense probably null
R5543:Pla2g15 UTSW 8 106161143 missense probably damaging 0.99
R6489:Pla2g15 UTSW 8 106163194 missense probably benign 0.10
R6802:Pla2g15 UTSW 8 106150581 missense probably damaging 0.99
R7381:Pla2g15 UTSW 8 106162944 missense probably benign 0.03
R7961:Pla2g15 UTSW 8 106160284 splice site probably null
Z1177:Pla2g15 UTSW 8 106162987 missense probably benign 0.19
Predicted Primers PCR Primer
(F):5'- TCTCTACCAGCTGGCTACTG -3'
(R):5'- GGCTAGCATCTCAATGTGCTCG -3'

Sequencing Primer
(F):5'- GCTACTGCCATACAACCACACTTG -3'
(R):5'- AGCTCCTGCAATGATACTCTGTG -3'
Posted On2015-10-21