Mutagenetix > Incidental Mutation

Incidental Mutation 'R4702:Or8b3b'

356142

Institutional Source

Beutler Lab

Gene Symbol

Or8b3b

Ensembl Gene

ENSMUSG00000046150

Gene Name

olfactory receptor family 8 subfamily B member 3B

Synonyms

GA_x6K02T2PVTD-32375756-32374818, MOR164-3, Olfr918

MMRRC Submission

041950-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.259) question?

Stock #

R4702 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

38583800-38584777 bp(-) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to T at 38584776 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 1 (M1K)

Ref Sequence

ENSEMBL: ENSMUSP00000057210 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055099] [ENSMUST00000215461]

AlphaFold

E9PVZ7

Predicted Effect

probably null
Transcript: ENSMUST00000055099
AA Change: M1K

SMART Domains

Protein: ENSMUSP00000057210
Gene: ENSMUSG00000046150
AA Change: M1K

Domain	Start	End	E-Value	Type
transmembrane domain	9	31	N/A	INTRINSIC
Pfam:7tm_4	44	319	6.5e-48	PFAM
Pfam:7tm_1	54	301	3.9e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000213750

Predicted Effect

probably benign
Transcript: ENSMUST00000215461

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216579

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921509C19Rik	T	C	2: 151,314,509 (GRCm39)	T390A	probably benign	Het
Adam25	G	T	8: 41,207,163 (GRCm39)	C143F	probably damaging	Het
Adra1a	T	C	14: 66,875,008 (GRCm39)		probably benign	Het
Aff1	T	C	5: 103,958,935 (GRCm39)	Y343H	probably damaging	Het
Antxr2	C	T	5: 98,097,028 (GRCm39)		probably null	Het
Ap1m2	A	G	9: 21,209,591 (GRCm39)	F362L	probably benign	Het
Apobec4	A	G	1: 152,632,001 (GRCm39)	T10A	probably benign	Het
Armc3	A	G	2: 19,314,792 (GRCm39)	N822S	probably damaging	Het
Atr	A	C	9: 95,802,408 (GRCm39)	T1767P	possibly damaging	Het
B4galnt1	G	T	10: 127,003,394 (GRCm39)	V172F	possibly damaging	Het
Bloc1s1	T	A	10: 128,759,267 (GRCm39)	Q13L	probably damaging	Het
Blvra	A	C	2: 126,933,982 (GRCm39)	I125L	probably benign	Het
Caps2	T	C	10: 112,044,252 (GRCm39)	F484L	probably damaging	Het
Cep76	A	T	18: 67,767,968 (GRCm39)	I188K	possibly damaging	Het
Cidea	T	A	18: 67,500,498 (GRCm39)	F187I	probably benign	Het
Cntn3	T	A	6: 102,142,292 (GRCm39)	N1025I	probably benign	Het
Cntnap3	A	T	13: 64,926,676 (GRCm39)	C565S	probably benign	Het
Cyp2d22	A	C	15: 82,260,118 (GRCm39)	L22R	probably damaging	Het
Cyp2d26	C	T	15: 82,676,648 (GRCm39)		probably benign	Het
Cyp2j12	A	T	4: 96,021,230 (GRCm39)		probably null	Het
Dnajb13	T	C	7: 100,153,748 (GRCm39)	N229S	probably benign	Het
Dpys	A	G	15: 39,656,798 (GRCm39)	V423A	possibly damaging	Het
Eif4e1b	A	T	13: 54,935,138 (GRCm39)	I222F	probably damaging	Het
Eif5b	A	T	1: 38,057,958 (GRCm39)	N87Y	unknown	Het
Enam	T	A	5: 88,651,650 (GRCm39)	L1053*	probably null	Het
Epha7	T	A	4: 28,961,425 (GRCm39)	L890Q	probably damaging	Het
Fancm	T	A	12: 65,168,826 (GRCm39)	S1730T	possibly damaging	Het
Flrt3	A	G	2: 140,503,575 (GRCm39)	F18L	probably benign	Het
Git2	A	G	5: 114,883,543 (GRCm39)	S396P	probably damaging	Het
H2-M10.4	A	G	17: 36,772,874 (GRCm39)	I36T	probably benign	Het
Igfn1	G	A	1: 135,894,947 (GRCm39)	S1873L	possibly damaging	Het
Ints12	A	T	3: 132,802,546 (GRCm39)	D10V	probably damaging	Het
Kbtbd2	A	G	6: 56,756,288 (GRCm39)	S483P	probably benign	Het
Kcnv2	G	C	19: 27,300,967 (GRCm39)	A273P	probably damaging	Het
Klc3	T	G	7: 19,129,756 (GRCm39)	D371A	probably damaging	Het
Lama3	T	A	18: 12,711,086 (GRCm39)	L1567*	probably null	Het
Ldhal6b	G	T	17: 5,468,582 (GRCm39)	H117Q	probably damaging	Het
Lrriq1	G	T	10: 103,051,610 (GRCm39)	Q381K	possibly damaging	Het
Mrps31	T	A	8: 22,909,754 (GRCm39)	L140Q	probably damaging	Het
Myo15b	A	G	11: 115,774,834 (GRCm39)	T2119A	probably benign	Het
Nkx6-2	T	C	7: 139,161,456 (GRCm39)	D243G	probably damaging	Het
Or1ab2	T	A	8: 72,864,044 (GRCm39)	F211L	probably damaging	Het
Papln	C	T	12: 83,828,757 (GRCm39)	T821I	probably benign	Het
Pitpnb	T	G	5: 111,519,218 (GRCm39)	V166G	probably benign	Het
Pla2g15	T	A	8: 106,889,691 (GRCm39)	M321K	probably benign	Het
Plcxd3	C	A	15: 4,405,269 (GRCm39)	S25R	probably benign	Het
Ppargc1a	T	C	5: 51,653,038 (GRCm39)	I175V	possibly damaging	Het
Ppp1r13b	T	A	12: 111,799,715 (GRCm39)	Q687H	probably benign	Het
Prpf3	A	G	3: 95,741,404 (GRCm39)	V584A	probably damaging	Het
Psen2	A	G	1: 180,055,289 (GRCm39)	L399S	probably damaging	Het
Ptchd3	G	A	11: 121,727,235 (GRCm39)	V370I	probably damaging	Het
Rasa3	G	T	8: 13,620,394 (GRCm39)	D758E	probably benign	Het
Reck	T	C	4: 43,898,060 (GRCm39)	C113R	probably damaging	Het
Resf1	A	G	6: 149,230,901 (GRCm39)	T1316A	probably benign	Het
Ric1	T	A	19: 29,575,417 (GRCm39)	F1009I	possibly damaging	Het
Rrp12	T	C	19: 41,859,975 (GRCm39)	N1035S	probably damaging	Het
Rtel1	T	A	2: 180,993,962 (GRCm39)	S849T	probably benign	Het
Scn10a	A	T	9: 119,462,857 (GRCm39)	Y1060N	possibly damaging	Het
Selenov	A	G	7: 27,987,436 (GRCm39)	L314P	probably damaging	Het
Selplg	T	C	5: 113,957,094 (GRCm39)	D404G	probably benign	Het
Slc7a14	T	A	3: 31,284,547 (GRCm39)	Y263F	probably damaging	Het
Slco3a1	A	G	7: 73,970,315 (GRCm39)	S431P	probably damaging	Het
Speer3	C	G	5: 13,846,394 (GRCm39)	A238G	possibly damaging	Het
Spopl	A	T	2: 23,405,309 (GRCm39)		probably null	Het
Stk10	A	T	11: 32,505,172 (GRCm39)	T69S	probably benign	Het
Tbxas1	T	C	6: 39,060,791 (GRCm39)		probably null	Het
Tec	G	A	5: 72,941,074 (GRCm39)	P161L	possibly damaging	Het
Tnip1	A	G	11: 54,815,228 (GRCm39)	S339P	probably benign	Het
Tsen34	T	A	7: 3,703,632 (GRCm39)	V290D	probably damaging	Het
Tuba1a	T	A	15: 98,849,563 (GRCm39)	I5F	possibly damaging	Het
Vmn1r31	C	A	6: 58,448,953 (GRCm39)	*304L	probably null	Het
Vmn1r63	T	C	7: 5,806,516 (GRCm39)	R39G	possibly damaging	Het
Xylt2	A	G	11: 94,560,355 (GRCm39)	Y307H	possibly damaging	Het
Zfp65	A	G	13: 67,872,341 (GRCm39)	M1T	probably null	Het
Zmym4	T	C	4: 126,816,958 (GRCm39)	I247V	probably benign	Het

Other mutations in Or8b3b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00958:Or8b3b	APN	9	38,584,320 (GRCm39)	missense	probably benign	0.01
IGL01388:Or8b3b	APN	9	38,584,379 (GRCm39)	nonsense	probably null
IGL01516:Or8b3b	APN	9	38,584,159 (GRCm39)	missense	probably benign	0.09
IGL02121:Or8b3b	APN	9	38,584,711 (GRCm39)	missense	probably damaging	0.98
IGL02209:Or8b3b	APN	9	38,584,342 (GRCm39)	missense	possibly damaging	0.84
IGL02256:Or8b3b	APN	9	38,584,776 (GRCm39)	start codon destroyed	probably null
IGL02517:Or8b3b	APN	9	38,584,209 (GRCm39)	missense	probably damaging	1.00
IGL02648:Or8b3b	APN	9	38,584,312 (GRCm39)	missense	probably benign
IGL02747:Or8b3b	APN	9	38,584,380 (GRCm39)	missense	probably benign	0.11
IGL02971:Or8b3b	APN	9	38,584,564 (GRCm39)	missense	probably damaging	0.96
E0370:Or8b3b	UTSW	9	38,583,857 (GRCm39)	missense	probably damaging	0.99
R0616:Or8b3b	UTSW	9	38,584,776 (GRCm39)	start codon destroyed	probably null
R2173:Or8b3b	UTSW	9	38,584,240 (GRCm39)	missense	probably benign	0.03
R2989:Or8b3b	UTSW	9	38,583,831 (GRCm39)	missense	probably benign
R3430:Or8b3b	UTSW	9	38,584,435 (GRCm39)	missense	probably damaging	1.00
R3809:Or8b3b	UTSW	9	38,584,159 (GRCm39)	missense	probably benign	0.09
R4688:Or8b3b	UTSW	9	38,584,659 (GRCm39)	missense	probably damaging	1.00
R5548:Or8b3b	UTSW	9	38,584,600 (GRCm39)	missense	probably benign	0.00
R5590:Or8b3b	UTSW	9	38,584,261 (GRCm39)	missense	probably damaging	1.00
R6082:Or8b3b	UTSW	9	38,583,866 (GRCm39)	missense	probably damaging	1.00
R6214:Or8b3b	UTSW	9	38,584,510 (GRCm39)	missense	probably benign	0.13
R6215:Or8b3b	UTSW	9	38,584,510 (GRCm39)	missense	probably benign	0.13
R6893:Or8b3b	UTSW	9	38,584,355 (GRCm39)	missense	possibly damaging	0.95
R7215:Or8b3b	UTSW	9	38,584,743 (GRCm39)	missense	probably benign	0.05
R7624:Or8b3b	UTSW	9	38,583,919 (GRCm39)	missense	probably benign	0.18
R7862:Or8b3b	UTSW	9	38,584,624 (GRCm39)	missense	probably benign	0.01
R8116:Or8b3b	UTSW	9	38,584,464 (GRCm39)	missense	possibly damaging	0.93
R8897:Or8b3b	UTSW	9	38,584,147 (GRCm39)	missense	probably damaging	1.00
R8899:Or8b3b	UTSW	9	38,584,147 (GRCm39)	missense	probably damaging	1.00
R9114:Or8b3b	UTSW	9	38,583,892 (GRCm39)	missense	probably benign
R9293:Or8b3b	UTSW	9	38,584,414 (GRCm39)	missense	probably damaging	1.00
R9311:Or8b3b	UTSW	9	38,583,925 (GRCm39)	missense	probably damaging	1.00
R9690:Or8b3b	UTSW	9	38,584,477 (GRCm39)	nonsense	probably null
R9734:Or8b3b	UTSW	9	38,584,239 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TCAGGATGATCAAGCCCAAGTTTC -3'
(R):5'- CCTCAATAAGTTTGCCAGTGGG -3'

Sequencing Primer
(F):5'- AGTTTCCCACCACTGTGACAG -3'
(R):5'- GCCAGTGGGTTTTATTTGATCATTC -3'

Posted On

2015-10-21