Incidental Mutation 'R3825:Tmem220'
ID 356345
Institutional Source Beutler Lab
Gene Symbol Tmem220
Ensembl Gene ENSMUSG00000050270
Gene Name transmembrane protein 220
Synonyms A730055C05Rik
MMRRC Submission 040774-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.074) question?
Stock # R3825 (G1)
Quality Score 72
Status Validated
Chromosome 11
Chromosomal Location 66915980-66926138 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 66916077 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Alanine to Threonine at position 25 (A25T)
Ref Sequence ENSEMBL: ENSMUSP00000078084 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000061786] [ENSMUST00000079077]
AlphaFold Q8BP07
Predicted Effect possibly damaging
Transcript: ENSMUST00000061786
AA Change: A25T

PolyPhen 2 Score 0.942 (Sensitivity: 0.80; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000057366
Gene: ENSMUSG00000050270
AA Change: A25T

DomainStartEndE-ValueType
Pfam:TMEM220 23 122 4e-30 PFAM
transmembrane domain 127 149 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000079077
AA Change: A25T

PolyPhen 2 Score 0.942 (Sensitivity: 0.80; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000078084
Gene: ENSMUSG00000050270
AA Change: A25T

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
Pfam:TMEM220 23 48 1.2e-9 PFAM
Pfam:TMEM220 64 144 4.3e-19 PFAM
transmembrane domain 151 173 N/A INTRINSIC
Meta Mutation Damage Score 0.1177 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.7%
  • 10x: 97.6%
  • 20x: 96.0%
Validation Efficiency 98% (63/64)
Allele List at MGI
Other mutations in this stock
Total: 62 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
6030468B19Rik A G 11: 117,693,739 (GRCm39) K69E probably damaging Het
Abcb5 C T 12: 118,865,087 (GRCm39) probably null Het
Acot1 G T 12: 84,061,194 (GRCm39) G167* probably null Het
Agbl1 C T 7: 76,069,715 (GRCm39) H164Y probably damaging Het
Atp4b T C 8: 13,443,549 (GRCm39) Y43C probably damaging Het
Bsn T C 9: 107,984,055 (GRCm39) D3333G unknown Het
Ccdc27 A C 4: 154,120,742 (GRCm39) D351E unknown Het
Cd200r4 T C 16: 44,641,313 (GRCm39) F19L probably benign Het
Dbndd1 T A 8: 124,236,731 (GRCm39) I75F probably damaging Het
Dennd5b T C 6: 148,946,334 (GRCm39) K426E probably benign Het
Drg2 A G 11: 60,350,334 (GRCm39) T98A possibly damaging Het
Fabp3 C T 4: 130,206,245 (GRCm39) probably null Het
Fmo1 T C 1: 162,678,916 (GRCm39) probably benign Het
Foxn2 A T 17: 88,791,837 (GRCm39) I236F probably damaging Het
Glmp G A 3: 88,233,718 (GRCm39) V107I probably damaging Het
Gpr15 A G 16: 58,538,723 (GRCm39) F122S probably damaging Het
Gvin-ps3 T C 7: 105,682,780 (GRCm39) I158M possibly damaging Het
Hmcn1 A T 1: 150,462,716 (GRCm39) D5140E probably benign Het
Hspa1a C T 17: 35,190,703 (GRCm39) V67M probably damaging Het
Igfbp4 A G 11: 98,939,061 (GRCm39) E27G probably damaging Het
Il7 A G 3: 7,642,226 (GRCm39) probably benign Het
Kat6a G T 8: 23,352,380 (GRCm39) V55F probably damaging Het
Kynu A G 2: 43,571,451 (GRCm39) T456A probably benign Het
Lcorl A G 5: 45,932,729 (GRCm39) probably benign Het
Lrp5 G A 19: 3,655,290 (GRCm39) R1077* probably null Het
Mab21l2 T C 3: 86,454,211 (GRCm39) E263G possibly damaging Het
Macf1 T A 4: 123,338,744 (GRCm39) D1436V probably benign Het
Map4k2 C A 19: 6,394,081 (GRCm39) T252N probably benign Het
Mterf2 A T 10: 84,956,147 (GRCm39) L159Q probably damaging Het
Muc5ac C A 7: 141,368,460 (GRCm39) T3063K possibly damaging Het
Myo18a T A 11: 77,668,292 (GRCm39) S51T possibly damaging Het
Ncoa3 G T 2: 165,896,718 (GRCm39) G503V possibly damaging Het
Ncor1 A T 11: 62,264,183 (GRCm39) H406Q probably benign Het
Nectin2 T C 7: 19,458,510 (GRCm39) K434E possibly damaging Het
Nipal2 A T 15: 34,578,852 (GRCm39) probably null Het
Nub1 G C 5: 24,912,851 (GRCm39) S517T probably benign Het
Or10a4 T C 7: 106,696,816 (GRCm39) L48P possibly damaging Het
Or4d10c A G 19: 12,065,391 (GRCm39) V255A probably damaging Het
Or8b101 A T 9: 38,020,134 (GRCm39) I51F possibly damaging Het
Or8c17 T G 9: 38,179,814 (GRCm39) S2A probably benign Het
Plscr3 G A 11: 69,740,964 (GRCm39) V267M probably benign Het
Plxnb2 G T 15: 89,050,602 (GRCm39) N451K probably benign Het
Ppp2r2a A G 14: 67,259,892 (GRCm39) L268P probably damaging Het
Ptprb A T 10: 116,186,694 (GRCm39) I1743F probably benign Het
Rack1 A G 11: 48,693,131 (GRCm39) T105A probably benign Het
Rhot1 G A 11: 80,116,907 (GRCm39) V94I probably damaging Het
Rin2 C T 2: 145,702,366 (GRCm39) T354I probably benign Het
Smc6 T A 12: 11,351,517 (GRCm39) probably benign Het
Spink8 T A 9: 109,645,861 (GRCm39) V11D probably damaging Het
Ss18l1 A T 2: 179,705,103 (GRCm39) Q365L unknown Het
Tbc1d31 T A 15: 57,779,474 (GRCm39) H62Q probably benign Het
Tbc1d9b T C 11: 50,061,954 (GRCm39) V1171A possibly damaging Het
Tln1 G A 4: 43,536,413 (GRCm39) probably benign Het
Tmem259 T C 10: 79,814,282 (GRCm39) N334S possibly damaging Het
Tmod3 A T 9: 75,414,809 (GRCm39) probably benign Het
Tmprss2 A T 16: 97,398,021 (GRCm39) Y52N probably damaging Het
Tsga10 A T 1: 37,873,278 (GRCm39) N200K possibly damaging Het
Ube2frt T C 12: 36,141,036 (GRCm39) probably benign Het
Vmn1r189 T A 13: 22,286,382 (GRCm39) T152S probably benign Het
Vmn2r76 C T 7: 85,880,415 (GRCm39) M90I probably benign Het
Zfr C T 15: 12,166,277 (GRCm39) A849V probably damaging Het
Znhit6 T C 3: 145,284,099 (GRCm39) M95T probably benign Het
Other mutations in Tmem220
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01074:Tmem220 APN 11 66,922,999 (GRCm39) splice site probably benign
IGL02191:Tmem220 APN 11 66,921,933 (GRCm39) missense probably damaging 1.00
IGL02364:Tmem220 APN 11 66,925,014 (GRCm39) missense probably benign 0.18
IGL02971:Tmem220 APN 11 66,924,933 (GRCm39) critical splice acceptor site probably null
R0497:Tmem220 UTSW 11 66,916,748 (GRCm39) missense probably damaging 1.00
R2024:Tmem220 UTSW 11 66,924,979 (GRCm39) missense possibly damaging 0.87
R4723:Tmem220 UTSW 11 66,920,819 (GRCm39) missense possibly damaging 0.89
R7229:Tmem220 UTSW 11 66,916,989 (GRCm39) missense unknown
R7939:Tmem220 UTSW 11 66,920,850 (GRCm39) missense probably damaging 1.00
R9461:Tmem220 UTSW 11 66,924,979 (GRCm39) missense possibly damaging 0.87
R9574:Tmem220 UTSW 11 66,916,093 (GRCm39) missense probably damaging 1.00
R9677:Tmem220 UTSW 11 66,925,011 (GRCm39) missense probably benign 0.38
R9718:Tmem220 UTSW 11 66,916,086 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGACCGTTCTAGACCTGAGG -3'
(R):5'- TCAAGCTCTGAGTGCGTTGC -3'

Sequencing Primer
(F):5'- AAGAAGGGCTCGCCTCG -3'
(R):5'- GAGTGCGTTGCCTAACTACAC -3'
Posted On 2015-10-28