Mutagenetix > Incidental Mutation

Incidental Mutation 'R0403:Speg'

35653

Institutional Source

Beutler Lab

Gene Symbol

Speg

Ensembl Gene

ENSMUSG00000026207

Gene Name

SPEG complex locus

Synonyms

SPEGbeta, Apeg1, SPEGalpha, D1Bwg1450e, SPEG, BPEG

MMRRC Submission

038608-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0403 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

75375297-75432320 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 75430784 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087122]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000087122

SMART Domains

Protein: ENSMUSP00000084361
Gene: ENSMUSG00000026207

Domain	Start	End	E-Value	Type
IG	51	128	1.48e-6	SMART
low complexity region	292	318	N/A	INTRINSIC
low complexity region	325	338	N/A	INTRINSIC
low complexity region	359	368	N/A	INTRINSIC
low complexity region	412	423	N/A	INTRINSIC
low complexity region	573	584	N/A	INTRINSIC
IGc2	739	806	2.19e-9	SMART
Pfam:SPEG_u2	817	873	2.4e-36	PFAM
IGc2	886	954	4.03e-8	SMART
IG	979	1064	1.05e-6	SMART
IGc2	1081	1148	2.19e-9	SMART
IG	1199	1283	6.87e-2	SMART
FN3	1287	1373	1.38e-4	SMART
IG	1401	1487	2.64e-3	SMART
IGc2	1502	1569	1.12e-6	SMART
STYKc	1606	1859	8.44e-63	SMART
Blast:STYKc	1861	1895	6e-12	BLAST
low complexity region	1918	1939	N/A	INTRINSIC
low complexity region	2069	2081	N/A	INTRINSIC
low complexity region	2208	2227	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2255	2269	N/A	INTRINSIC
low complexity region	2343	2366	N/A	INTRINSIC
low complexity region	2410	2422	N/A	INTRINSIC
low complexity region	2433	2451	N/A	INTRINSIC
low complexity region	2457	2487	N/A	INTRINSIC
low complexity region	2524	2544	N/A	INTRINSIC
IGc2	2599	2667	2.05e-9	SMART
FN3	2681	2760	2.5e-2	SMART
low complexity region	2775	2789	N/A	INTRINSIC
low complexity region	2802	2831	N/A	INTRINSIC
low complexity region	2912	2927	N/A	INTRINSIC
STYKc	2961	3213	4.42e-66	SMART
low complexity region	3241	3250	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000137868

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143679

Coding Region Coverage

1x: 98.0%
3x: 96.8%
10x: 93.2%
20x: 83.7%

Validation Efficiency

97% (110/113)

MGI Phenotype

FUNCTION: This gene encodes a protein with similarity to members of the myosin light chain kinase family. This protein family is required for myocyte cytoskeletal development. Studies have determined that a lack of this protein affected myocardial development. Multiple alternatively spliced transcript variants that encode different protein isoforms have been defined. [provided by RefSeq, Mar 2010]
PHENOTYPE: Mice homozygous for a knock-out allele die during the early postnatal period with enlarged, dilated hearts, and decreased cardiac function. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 105 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ace	A	G	11: 105,973,880 (GRCm38)		probably null	Het
Adgrg3	C	T	8: 95,036,922 (GRCm38)	L284F	probably benign	Het
Alkbh8	T	A	9: 3,385,469 (GRCm38)	V587E	probably damaging	Het
Ap4b1	T	A	3: 103,818,839 (GRCm38)	C244S	probably damaging	Het
Ap4b1	T	A	3: 103,821,396 (GRCm38)	M590K	probably benign	Het
Arhgap15	C	T	2: 44,063,766 (GRCm38)	T168I	probably damaging	Het
Atp8b1	A	G	18: 64,540,310 (GRCm38)	V997A	probably damaging	Het
Atrn	G	A	2: 130,906,859 (GRCm38)	C100Y	probably damaging	Het
Baiap2l2	C	A	15: 79,271,216 (GRCm38)	A151S	probably benign	Het
Baz2b	A	T	2: 59,969,377 (GRCm38)	D199E	possibly damaging	Het
Bmal2	T	A	6: 146,822,655 (GRCm38)	H348Q	probably damaging	Het
Cblb	A	G	16: 52,152,626 (GRCm38)	D440G	probably benign	Het
Cdon	T	C	9: 35,473,500 (GRCm38)	V694A	probably benign	Het
Cep250	A	T	2: 155,992,349 (GRCm38)	R2065W	probably damaging	Het
Ces2b	G	T	8: 104,833,945 (GRCm38)	A131S	probably damaging	Het
Chrna9	A	T	5: 65,967,892 (GRCm38)	T59S	possibly damaging	Het
Cog3	T	A	14: 75,742,327 (GRCm38)		probably benign	Het
Cpa1	G	A	6: 30,641,857 (GRCm38)	V227I	probably benign	Het
Cyp3a25	A	T	5: 145,998,513 (GRCm38)	C98S	probably damaging	Het
D8Ertd738e	C	T	8: 84,249,601 (GRCm38)		probably null	Het
Ddx60	A	G	8: 61,994,541 (GRCm38)		probably benign	Het
Dhx16	T	C	17: 35,883,050 (GRCm38)		probably null	Het
Dnah9	T	A	11: 66,084,789 (GRCm38)	Q1478L	possibly damaging	Het
Dock10	T	C	1: 80,524,070 (GRCm38)	Y1434C	possibly damaging	Het
Enpp3	T	A	10: 24,804,436 (GRCm38)	D325V	probably damaging	Het
Entpd6	C	A	2: 150,760,170 (GRCm38)	T194K	possibly damaging	Het
Fat2	A	T	11: 55,270,349 (GRCm38)	V3185E	probably benign	Het
Flrt1	A	G	19: 7,095,919 (GRCm38)	L421P	probably benign	Het
Fmn2	A	T	1: 174,694,278 (GRCm38)	Q1292L	probably damaging	Het
Fndc1	T	C	17: 7,775,588 (GRCm38)		probably null	Het
Fndc1	T	C	17: 7,753,723 (GRCm38)	D1459G	probably damaging	Het
Fzr1	A	G	10: 81,369,368 (GRCm38)	S265P	possibly damaging	Het
Gpr142	G	A	11: 114,806,029 (GRCm38)	V134M	probably damaging	Het
Grid2ip	G	T	5: 143,357,620 (GRCm38)	V24L	possibly damaging	Het
Herc2	G	A	7: 56,159,417 (GRCm38)	R2555H	probably damaging	Het
Hpdl	A	T	4: 116,820,479 (GRCm38)	Y262N	possibly damaging	Het
Htr3a	A	G	9: 48,908,659 (GRCm38)	V57A	probably damaging	Het
Igfbp7	T	C	5: 77,355,591 (GRCm38)	I186V	probably benign	Het
Itga2b	C	T	11: 102,467,326 (GRCm38)		probably null	Het
Itgae	A	C	11: 73,123,183 (GRCm38)	D736A	possibly damaging	Het
Itpkc	T	A	7: 27,208,345 (GRCm38)	M645L	probably benign	Het
Jchain	T	C	5: 88,521,378 (GRCm38)	R139G	probably benign	Het
Kif13a	A	G	13: 46,791,401 (GRCm38)	V908A	probably damaging	Het
Kif1b	T	A	4: 149,181,967 (GRCm38)	K389*	probably null	Het
Klhl12	T	C	1: 134,485,856 (GRCm38)	Y360H	possibly damaging	Het
Knop1	G	A	7: 118,853,053 (GRCm38)	R148W	probably damaging	Het
Lpar1	T	A	4: 58,487,191 (GRCm38)	N27Y	probably damaging	Het
Lpar2	T	A	8: 69,824,152 (GRCm38)	V197D	probably damaging	Het
Lrrc74a	A	G	12: 86,740,979 (GRCm38)	N128S	probably damaging	Het
Lum	G	T	10: 97,572,043 (GRCm38)	V337F	probably benign	Het
Mag	T	C	7: 30,906,980 (GRCm38)	D344G	probably damaging	Het
Maip1	G	A	1: 57,407,196 (GRCm38)	A142T	probably benign	Het
Mlh3	A	G	12: 85,268,968 (GRCm38)	V148A	possibly damaging	Het
Nav3	A	G	10: 109,767,103 (GRCm38)	V1195A	probably damaging	Het
Ncor2	A	G	5: 125,033,337 (GRCm38)	S868P	possibly damaging	Het
Nek1	A	G	8: 61,106,855 (GRCm38)	E907G	probably damaging	Het
Nfam1	G	A	15: 83,016,379 (GRCm38)	T134I	probably benign	Het
Nr0b2	T	C	4: 133,553,759 (GRCm38)	V112A	probably damaging	Het
Nrp1	A	G	8: 128,457,969 (GRCm38)	N365S	probably damaging	Het
Nrsn2	T	C	2: 152,369,790 (GRCm38)	Y107C	probably damaging	Het
Ntng1	G	A	3: 109,934,611 (GRCm38)	A282V	probably damaging	Het
Nxf1	T	C	19: 8,765,028 (GRCm38)	I337T	probably damaging	Het
Obscn	C	T	11: 59,076,540 (GRCm38)	G479D	probably damaging	Het
Oprd1	T	A	4: 132,113,768 (GRCm38)	D293V	probably benign	Het
Or13a17	T	C	7: 140,691,309 (GRCm38)	S135P	possibly damaging	Het
P3h2	T	G	16: 25,969,950 (GRCm38)	N586H	possibly damaging	Het
Pcid2	T	C	8: 13,085,367 (GRCm38)	Y214C	probably damaging	Het
Pkd1l3	C	G	8: 109,623,649 (GRCm38)	D375E	possibly damaging	Het
Pkd1l3	G	A	8: 109,623,663 (GRCm38)	S380N	probably benign	Het
Ppic	C	A	18: 53,411,071 (GRCm38)	G81W	probably damaging	Het
Ppp2r1a	C	A	17: 20,957,041 (GRCm38)	P246T	probably damaging	Het
Ppp4r4	T	G	12: 103,584,102 (GRCm38)	S46A	probably benign	Het
Pramel31	A	G	4: 144,362,646 (GRCm38)	N178S	probably benign	Het
Prkce	A	G	17: 86,168,653 (GRCm38)	T21A	probably damaging	Het
Prkg2	T	C	5: 98,994,645 (GRCm38)	E210G	possibly damaging	Het
Prss35	A	G	9: 86,756,037 (GRCm38)	M287V	probably damaging	Het
Psd	G	A	19: 46,320,972 (GRCm38)		probably benign	Het
Ptch2	A	T	4: 117,110,839 (GRCm38)	K843*	probably null	Het
Rab44	C	A	17: 29,145,261 (GRCm38)	T603K	probably damaging	Het
Rasal3	T	A	17: 32,392,790 (GRCm38)		probably null	Het
Rbbp6	A	G	7: 122,992,296 (GRCm38)	T526A	probably damaging	Het
Ros1	A	T	10: 52,143,438 (GRCm38)		probably benign	Het
Sec24b	A	G	3: 129,999,534 (GRCm38)	S685P	probably damaging	Het
Sec24b	T	A	3: 129,989,676 (GRCm38)	L1104F	possibly damaging	Het
Sema6a	G	A	18: 47,290,045 (GRCm38)		probably null	Het
Setmar	A	T	6: 108,075,962 (GRCm38)	H139L	probably benign	Het
Slc28a2b	T	C	2: 122,521,854 (GRCm38)	L364S	probably damaging	Het
Slc4a7	T	C	14: 14,766,808 (GRCm38)	V710A	probably benign	Het
Smarcc1	T	A	9: 110,237,808 (GRCm38)		probably null	Het
Smchd1	G	A	17: 71,394,902 (GRCm38)	L1032F	probably damaging	Het
Tasor2	A	T	13: 3,582,052 (GRCm38)	Y816*	probably null	Het
Tcea1	C	G	1: 4,889,503 (GRCm38)	R134G	probably benign	Het
Tchhl1	C	T	3: 93,471,029 (GRCm38)	Q347*	probably null	Het
Tecrl	A	T	5: 83,354,758 (GRCm38)		probably benign	Het
Tepsin	T	C	11: 120,093,682 (GRCm38)		probably benign	Het
Tmem40	G	A	6: 115,733,985 (GRCm38)		probably benign	Het
Tpr	G	A	1: 150,407,414 (GRCm38)		probably benign	Het
Ttll12	A	T	15: 83,580,658 (GRCm38)		probably benign	Het
Ttn	T	A	2: 76,909,608 (GRCm38)	D3529V	probably benign	Het
Usp34	T	A	11: 23,333,838 (GRCm38)	H177Q	possibly damaging	Het
Vsig10	T	A	5: 117,338,461 (GRCm38)	S327T	probably benign	Het
Zbtb4	T	A	11: 69,777,639 (GRCm38)	M396K	probably damaging	Het
Zfp352	C	T	4: 90,225,009 (GRCm38)	T462I	possibly damaging	Het
Zfp385b	T	C	2: 77,476,845 (GRCm38)	M145V	probably damaging	Het
Zfp780b	C	A	7: 27,971,689 (GRCm38)	V65F	possibly damaging	Het

Other mutations in Speg

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00838:Speg	APN	1	75,410,390 (GRCm38)	missense	possibly damaging	0.95
IGL00979:Speg	APN	1	75,410,734 (GRCm38)	missense	probably damaging	0.98
IGL01122:Speg	APN	1	75,410,035 (GRCm38)	missense	probably damaging	1.00
IGL01293:Speg	APN	1	75,388,102 (GRCm38)	missense	probably damaging	1.00
IGL01304:Speg	APN	1	75,428,197 (GRCm38)	missense	probably benign	0.00
IGL01351:Speg	APN	1	75,411,276 (GRCm38)	splice site	probably benign
IGL01473:Speg	APN	1	75,428,285 (GRCm38)	missense	possibly damaging	0.53
IGL01477:Speg	APN	1	75,391,897 (GRCm38)	missense	probably damaging	1.00
IGL01485:Speg	APN	1	75,387,827 (GRCm38)	missense	probably damaging	1.00
IGL01584:Speg	APN	1	75,430,937 (GRCm38)	missense	probably damaging	1.00
IGL01959:Speg	APN	1	75,391,090 (GRCm38)	missense	probably damaging	1.00
IGL02231:Speg	APN	1	75,423,387 (GRCm38)	missense	probably damaging	1.00
IGL02355:Speg	APN	1	75,423,915 (GRCm38)	missense	possibly damaging	0.49
IGL02362:Speg	APN	1	75,423,915 (GRCm38)	missense	possibly damaging	0.49
IGL03013:Speg	APN	1	75,431,279 (GRCm38)	missense	probably damaging	0.97
IGL03168:Speg	APN	1	75,388,187 (GRCm38)	missense	probably damaging	1.00
H8562:Speg	UTSW	1	75,415,597 (GRCm38)	missense	probably benign	0.39
R0112:Speg	UTSW	1	75,385,032 (GRCm38)	missense	possibly damaging	0.92
R0311:Speg	UTSW	1	75,430,937 (GRCm38)	missense	probably damaging	1.00
R0315:Speg	UTSW	1	75,415,136 (GRCm38)	missense	possibly damaging	0.88
R0393:Speg	UTSW	1	75,423,924 (GRCm38)	missense	possibly damaging	0.46
R0483:Speg	UTSW	1	75,385,032 (GRCm38)	missense	possibly damaging	0.92
R0648:Speg	UTSW	1	75,427,978 (GRCm38)	missense	probably benign
R0683:Speg	UTSW	1	75,429,118 (GRCm38)	missense	probably damaging	1.00
R0800:Speg	UTSW	1	75,423,489 (GRCm38)	missense	probably damaging	1.00
R0815:Speg	UTSW	1	75,415,392 (GRCm38)	missense	probably damaging	1.00
R0835:Speg	UTSW	1	75,375,674 (GRCm38)	missense	probably benign	0.00
R0866:Speg	UTSW	1	75,417,083 (GRCm38)	missense	probably damaging	0.99
R0880:Speg	UTSW	1	75,405,061 (GRCm38)	missense	probably damaging	1.00
R1082:Speg	UTSW	1	75,415,138 (GRCm38)	missense	possibly damaging	0.94
R1140:Speg	UTSW	1	75,429,095 (GRCm38)	missense	probably damaging	1.00
R1252:Speg	UTSW	1	75,427,095 (GRCm38)	missense	probably damaging	1.00
R1301:Speg	UTSW	1	75,401,501 (GRCm38)	missense	probably damaging	1.00
R1348:Speg	UTSW	1	75,422,872 (GRCm38)	missense	probably damaging	0.99
R1388:Speg	UTSW	1	75,430,460 (GRCm38)	missense	probably damaging	0.99
R1465:Speg	UTSW	1	75,428,484 (GRCm38)	splice site	probably benign
R1505:Speg	UTSW	1	75,375,542 (GRCm38)	missense	probably benign	0.02
R1506:Speg	UTSW	1	75,417,663 (GRCm38)	missense	probably benign	0.03
R1531:Speg	UTSW	1	75,401,222 (GRCm38)	missense	possibly damaging	0.86
R1543:Speg	UTSW	1	75,421,951 (GRCm38)	missense	probably damaging	1.00
R1567:Speg	UTSW	1	75,428,047 (GRCm38)	missense	probably benign
R1630:Speg	UTSW	1	75,422,977 (GRCm38)	missense	probably damaging	1.00
R1667:Speg	UTSW	1	75,410,549 (GRCm38)	splice site	probably benign
R1673:Speg	UTSW	1	75,411,163 (GRCm38)	missense	possibly damaging	0.60
R1718:Speg	UTSW	1	75,421,744 (GRCm38)	missense	possibly damaging	0.87
R1718:Speg	UTSW	1	75,417,863 (GRCm38)	missense	probably benign	0.00
R1719:Speg	UTSW	1	75,417,863 (GRCm38)	missense	probably benign	0.00
R1759:Speg	UTSW	1	75,401,162 (GRCm38)	missense	possibly damaging	0.95
R1861:Speg	UTSW	1	75,389,005 (GRCm38)	missense	probably damaging	1.00
R1874:Speg	UTSW	1	75,423,906 (GRCm38)	missense	probably benign
R1936:Speg	UTSW	1	75,431,408 (GRCm38)	missense	possibly damaging	0.93
R2192:Speg	UTSW	1	75,417,727 (GRCm38)	missense	probably damaging	1.00
R2204:Speg	UTSW	1	75,430,477 (GRCm38)	missense	probably benign	0.30
R2287:Speg	UTSW	1	75,430,465 (GRCm38)	missense	possibly damaging	0.76
R2696:Speg	UTSW	1	75,406,926 (GRCm38)	missense	probably benign	0.27
R2983:Speg	UTSW	1	75,384,930 (GRCm38)	missense	possibly damaging	0.83
R3110:Speg	UTSW	1	75,422,682 (GRCm38)	nonsense	probably null
R3112:Speg	UTSW	1	75,422,682 (GRCm38)	nonsense	probably null
R3154:Speg	UTSW	1	75,401,542 (GRCm38)	missense	probably damaging	1.00
R3720:Speg	UTSW	1	75,426,782 (GRCm38)	missense	probably damaging	1.00
R3983:Speg	UTSW	1	75,422,547 (GRCm38)	missense	probably benign	0.27
R4133:Speg	UTSW	1	75,427,904 (GRCm38)	missense	probably benign
R4522:Speg	UTSW	1	75,428,330 (GRCm38)	missense	probably damaging	1.00
R4564:Speg	UTSW	1	75,391,834 (GRCm38)	missense	probably damaging	1.00
R4577:Speg	UTSW	1	75,415,395 (GRCm38)	missense	probably damaging	1.00
R4858:Speg	UTSW	1	75,421,735 (GRCm38)	missense	probably damaging	1.00
R4953:Speg	UTSW	1	75,423,864 (GRCm38)	missense	possibly damaging	0.72
R4965:Speg	UTSW	1	75,427,703 (GRCm38)	missense	probably damaging	1.00
R4967:Speg	UTSW	1	75,387,869 (GRCm38)	missense	probably damaging	1.00
R5152:Speg	UTSW	1	75,428,098 (GRCm38)	missense	possibly damaging	0.92
R5156:Speg	UTSW	1	75,428,087 (GRCm38)	missense	probably damaging	0.99
R5371:Speg	UTSW	1	75,431,393 (GRCm38)	missense	possibly damaging	0.50
R5550:Speg	UTSW	1	75,429,100 (GRCm38)	missense	probably damaging	1.00
R5562:Speg	UTSW	1	75,427,056 (GRCm38)	missense	probably damaging	1.00
R5687:Speg	UTSW	1	75,419,129 (GRCm38)	splice site	probably null
R5985:Speg	UTSW	1	75,406,684 (GRCm38)	missense	possibly damaging	0.94
R6004:Speg	UTSW	1	75,415,603 (GRCm38)	nonsense	probably null
R6038:Speg	UTSW	1	75,418,459 (GRCm38)	critical splice donor site	probably null
R6038:Speg	UTSW	1	75,418,459 (GRCm38)	critical splice donor site	probably null
R6143:Speg	UTSW	1	75,414,387 (GRCm38)	missense	probably damaging	1.00
R6265:Speg	UTSW	1	75,406,679 (GRCm38)	nonsense	probably null
R6347:Speg	UTSW	1	75,426,875 (GRCm38)	missense	probably benign	0.00
R6453:Speg	UTSW	1	75,417,972 (GRCm38)	missense	probably benign	0.06
R6505:Speg	UTSW	1	75,429,523 (GRCm38)	missense	possibly damaging	0.93
R6505:Speg	UTSW	1	75,406,684 (GRCm38)	missense	possibly damaging	0.94
R6531:Speg	UTSW	1	75,422,757 (GRCm38)	missense	probably benign	0.03
R6566:Speg	UTSW	1	75,388,463 (GRCm38)	missense	probably damaging	1.00
R6747:Speg	UTSW	1	75,410,395 (GRCm38)	critical splice donor site	probably null
R6819:Speg	UTSW	1	75,391,812 (GRCm38)	missense	possibly damaging	0.56
R6821:Speg	UTSW	1	75,417,903 (GRCm38)	missense	possibly damaging	0.83
R6919:Speg	UTSW	1	75,387,908 (GRCm38)	nonsense	probably null
R6981:Speg	UTSW	1	75,430,913 (GRCm38)	missense	probably damaging	1.00
R7002:Speg	UTSW	1	75,423,268 (GRCm38)	missense	probably damaging	0.98
R7082:Speg	UTSW	1	75,411,447 (GRCm38)	missense	probably damaging	0.96
R7140:Speg	UTSW	1	75,406,770 (GRCm38)	critical splice donor site	probably null
R7175:Speg	UTSW	1	75,422,490 (GRCm38)	missense	probably benign	0.01
R7178:Speg	UTSW	1	75,422,383 (GRCm38)	missense	possibly damaging	0.46
R7345:Speg	UTSW	1	75,384,835 (GRCm38)	missense	probably damaging	0.97
R7420:Speg	UTSW	1	75,430,905 (GRCm38)	missense	probably damaging	1.00
R7537:Speg	UTSW	1	75,401,464 (GRCm38)	missense	probably damaging	1.00
R7562:Speg	UTSW	1	75,431,279 (GRCm38)	missense	probably damaging	0.97
R7615:Speg	UTSW	1	75,429,242 (GRCm38)	missense	probably damaging	1.00
R7679:Speg	UTSW	1	75,406,315 (GRCm38)	missense	probably damaging	1.00
R7692:Speg	UTSW	1	75,401,190 (GRCm38)	missense	probably benign	0.04
R7696:Speg	UTSW	1	75,429,161 (GRCm38)	missense	probably damaging	1.00
R7719:Speg	UTSW	1	75,375,825 (GRCm38)	missense	probably damaging	1.00
R7794:Speg	UTSW	1	75,388,870 (GRCm38)	missense	probably benign	0.00
R7824:Speg	UTSW	1	75,384,017 (GRCm38)	splice site	probably null
R7834:Speg	UTSW	1	75,384,927 (GRCm38)	missense	probably damaging	1.00
R7892:Speg	UTSW	1	75,427,166 (GRCm38)	missense	probably damaging	1.00
R8015:Speg	UTSW	1	75,415,421 (GRCm38)	splice site	probably benign
R8068:Speg	UTSW	1	75,422,250 (GRCm38)	missense	probably damaging	1.00
R8085:Speg	UTSW	1	75,415,353 (GRCm38)	missense	probably damaging	1.00
R8130:Speg	UTSW	1	75,415,596 (GRCm38)	missense	probably damaging	1.00
R8132:Speg	UTSW	1	75,422,995 (GRCm38)	missense	probably damaging	1.00
R8239:Speg	UTSW	1	75,419,033 (GRCm38)	missense	probably damaging	1.00
R8287:Speg	UTSW	1	75,422,236 (GRCm38)	missense	probably benign	0.26
R8299:Speg	UTSW	1	75,387,836 (GRCm38)	missense	possibly damaging	0.95
R8441:Speg	UTSW	1	75,411,332 (GRCm38)	missense	possibly damaging	0.60
R8468:Speg	UTSW	1	75,431,309 (GRCm38)	missense	probably damaging	1.00
R8555:Speg	UTSW	1	75,402,264 (GRCm38)	splice site	probably null
R8781:Speg	UTSW	1	75,407,021 (GRCm38)	missense	probably damaging	1.00
R8784:Speg	UTSW	1	75,405,149 (GRCm38)	critical splice donor site	probably benign
R8848:Speg	UTSW	1	75,427,438 (GRCm38)	critical splice donor site	probably null
R8881:Speg	UTSW	1	75,401,151 (GRCm38)	missense	possibly damaging	0.67
R8898:Speg	UTSW	1	75,388,873 (GRCm38)	missense	probably damaging	1.00
R8935:Speg	UTSW	1	75,422,606 (GRCm38)	missense	probably benign	0.30
R9019:Speg	UTSW	1	75,429,238 (GRCm38)	missense	probably damaging	1.00
R9027:Speg	UTSW	1	75,388,432 (GRCm38)	missense	possibly damaging	0.67
R9066:Speg	UTSW	1	75,385,010 (GRCm38)	missense	probably damaging	0.99
R9092:Speg	UTSW	1	75,422,734 (GRCm38)	missense	probably benign	0.01
R9117:Speg	UTSW	1	75,387,800 (GRCm38)	missense	probably damaging	1.00
R9202:Speg	UTSW	1	75,390,993 (GRCm38)	missense	probably damaging	1.00
R9246:Speg	UTSW	1	75,384,854 (GRCm38)	missense	probably damaging	1.00
R9248:Speg	UTSW	1	75,421,776 (GRCm38)	missense	probably damaging	1.00
R9451:Speg	UTSW	1	75,417,733 (GRCm38)	missense	probably damaging	1.00
R9452:Speg	UTSW	1	75,422,508 (GRCm38)	missense	probably benign
R9475:Speg	UTSW	1	75,388,091 (GRCm38)	missense	probably damaging	1.00
R9476:Speg	UTSW	1	75,401,124 (GRCm38)	missense	probably damaging	0.99
R9510:Speg	UTSW	1	75,401,124 (GRCm38)	missense	probably damaging	0.99
R9519:Speg	UTSW	1	75,415,736 (GRCm38)	missense	probably damaging	1.00
R9528:Speg	UTSW	1	75,387,803 (GRCm38)	missense	possibly damaging	0.78
R9542:Speg	UTSW	1	75,422,782 (GRCm38)	missense	probably benign	0.08
R9553:Speg	UTSW	1	75,418,001 (GRCm38)	missense	probably benign	0.00
R9767:Speg	UTSW	1	75,427,181 (GRCm38)	missense	possibly damaging	0.78
R9768:Speg	UTSW	1	75,418,973 (GRCm38)	nonsense	probably null
R9800:Speg	UTSW	1	75,422,714 (GRCm38)	missense	probably benign	0.03
X0025:Speg	UTSW	1	75,422,457 (GRCm38)	missense	probably damaging	1.00
X0026:Speg	UTSW	1	75,423,475 (GRCm38)	missense	possibly damaging	0.88
Z1176:Speg	UTSW	1	75,406,594 (GRCm38)	missense	probably damaging	1.00
Z1177:Speg	UTSW	1	75,427,683 (GRCm38)	missense	probably damaging	1.00
Z1177:Speg	UTSW	1	75,430,455 (GRCm38)	missense	probably damaging	0.99
Z1177:Speg	UTSW	1	75,428,381 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTCTTGTCAGCTCCTGAGATGGTG -3'
(R):5'- CGGACTTTAAATGGACGGCAGTGTG -3'

Sequencing Primer
(F):5'- ATTGGCTCTGCCACGGAC -3'
(R):5'- CATGGTGGTCTGCATTCAGAAATAG -3'

Posted On

2013-05-09