Mutagenetix > Incidental Mutation

Incidental Mutation 'R4744:Olfr345'

356550

Institutional Source

Beutler Lab

Gene Symbol

Olfr345

Ensembl Gene

ENSMUSG00000059251

Gene Name

olfactory receptor 345

Synonyms

GA_x6K02T2NLDC-33334179-33335114, MOR136-7

MMRRC Submission

042027-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R4744 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

36640041-36640976 bp(+) (GRCm38)

Type of Mutation

splice site (3 bp from exon)

DNA Base Change (assembly)

A to T at 36640979 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000076122 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076850]

AlphaFold

Q8VGK4

Predicted Effect

probably null
Transcript: ENSMUST00000076850

SMART Domains

Protein: ENSMUSP00000076122
Gene: ENSMUSG00000059251

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	8.6e-57	PFAM
Pfam:7tm_1	41	290	2.6e-22	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

99% (82/83)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930519G04Rik	T	C	5: 114,879,556	S143P	possibly damaging	Het
4933427D14Rik	T	C	11: 72,175,539	K614E	probably damaging	Het
Aatk	A	G	11: 120,016,122	M155T	possibly damaging	Het
Acvr2b	T	C	9: 119,431,262	L333P	probably damaging	Het
Adam22	T	C	5: 8,078,699	E865G	probably damaging	Het
Add2	A	G	6: 86,110,888	S358G	probably damaging	Het
Agap2	T	C	10: 127,090,203		probably null	Het
Alkbh8	G	A	9: 3,344,604	W49*	probably null	Het
AU015228	A	T	2: 130,100,629		noncoding transcript	Het
Bank1	G	T	3: 136,247,689	R102S	probably benign	Het
Brap	T	A	5: 121,662,130	D27E	probably damaging	Het
Cyb5r2	G	T	7: 107,750,277	H276N	possibly damaging	Het
Dhh	A	G	15: 98,894,258	F290L	possibly damaging	Het
Dhrs7	T	A	12: 72,652,251	N319I	possibly damaging	Het
Ebpl	A	G	14: 61,360,233	V53A	probably damaging	Het
Eif3j2	TGCCGCCGCCGCCGCCGCCGCCGCCGCC	TGCCGCCGCCGCCGCCGCCGCCGCC	18: 43,477,717		probably benign	Het
Etnk1	A	C	6: 143,186,593	N220T	probably damaging	Het
F11r	T	A	1: 171,460,598	V64D	probably benign	Het
Fam171a1	T	C	2: 3,224,909	S360P	probably damaging	Het
Fignl1	A	G	11: 11,801,585	M490T	probably damaging	Het
Fpr-rs7	A	T	17: 20,114,003	M75K	probably benign	Het
Fzd7	T	A	1: 59,484,436	F493I	possibly damaging	Het
Galnt14	G	T	17: 73,507,833	P412T	probably damaging	Het
Gcg	T	A	2: 62,478,631	S60C	probably damaging	Het
Ggt1	A	G	10: 75,585,899	K527E	probably benign	Het
Gm14085	A	T	2: 122,522,805	K489*	probably null	Het
Gm16551	T	A	9: 74,850,871		noncoding transcript	Het
Gm9972	A	G	11: 43,036,690	K55E	unknown	Het
Gpr141	T	A	13: 19,751,714	D297V	probably benign	Het
Grin2b	G	A	6: 135,778,699	S539L	probably damaging	Het
Hhipl1	A	T	12: 108,319,979	N515I	possibly damaging	Het
Hmcn1	T	G	1: 150,577,612	E5317D	probably damaging	Het
Hsf5	T	A	11: 87,622,791	N227K	probably benign	Het
Igfn1	A	T	1: 135,982,458	D129E	probably benign	Het
Invs	T	C	4: 48,397,609	F339L	probably damaging	Het
Jak2	T	A	19: 29,262,256	S17T	probably benign	Het
Mdga2	T	A	12: 66,797,727	I166F	probably benign	Het
Nck1	T	C	9: 100,506,744	I6V	probably benign	Het
Neb	T	C	2: 52,150,577	D6624G	probably benign	Het
Nmur2	A	G	11: 56,040,835	Y17H	probably benign	Het
Nwd2	T	C	5: 63,806,967	L1298P	probably damaging	Het
Ocel1	A	G	8: 71,372,753	E161G	probably damaging	Het
Pabpc2	A	G	18: 39,774,828	Y382C	probably benign	Het
Panx1	A	G	9: 15,010,298		probably benign	Het
Pdhx	A	T	2: 103,042,296	V147D	probably benign	Het
Pigz	A	T	16: 31,945,333	H403L	probably damaging	Het
Pilra	T	C	5: 137,835,507		probably null	Het
Rbm47	T	C	5: 66,026,693	D189G	probably damaging	Het
Rhobtb2	A	G	14: 69,794,002	L558P	probably damaging	Het
Scarf2	G	A	16: 17,803,516	R322H	probably damaging	Het
Sept3	T	C	15: 82,290,457		probably null	Het
Sirt2	T	C	7: 28,777,013	F26L	probably damaging	Het
Slc1a1	A	T	19: 28,894,525	T133S	probably benign	Het
Slc1a2	A	G	2: 102,737,869	I84V	probably benign	Het
Slc6a9	A	T	4: 117,867,895	Q562L	probably benign	Het
Snx29	C	T	16: 11,349,909	Q25*	probably null	Het
St6galnac6	A	G	2: 32,618,543	I231V	probably damaging	Het
Stard9	A	G	2: 120,696,123	T954A	probably benign	Het
Sufu	A	G	19: 46,483,630	M443V	possibly damaging	Het
Sv2b	T	C	7: 75,206,518	D8G	probably benign	Het
Tapbpl	G	A	6: 125,228,285	R233W	probably damaging	Het
Tex10	A	G	4: 48,469,990	L25S	probably benign	Het
Trp53bp1	A	T	2: 121,211,313	V1254D	probably damaging	Het
Ugt3a1	T	A	15: 9,310,553	I307N	probably benign	Het
Unc13c	T	C	9: 73,931,844	D575G	probably damaging	Het
Usf2	A	T	7: 30,954,772	D166E	probably damaging	Het
Usp25	T	A	16: 77,114,989	L969M	probably damaging	Het
Usp32	G	A	11: 84,994,393	P1276L	probably damaging	Het
Vmn2r8	T	A	5: 108,808,581	E58D	probably benign	Het
Zfp462	G	T	4: 55,011,598	C40F	probably damaging	Het

Other mutations in Olfr345

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03323:Olfr345	APN	2	36640141	missense	possibly damaging	0.95
IGL03399:Olfr345	APN	2	36640882	missense	possibly damaging	0.78
R1164:Olfr345	UTSW	2	36640120	missense	probably benign
R1878:Olfr345	UTSW	2	36640189	missense	possibly damaging	0.83
R1879:Olfr345	UTSW	2	36640189	missense	possibly damaging	0.83
R1954:Olfr345	UTSW	2	36640215	missense	possibly damaging	0.86
R2206:Olfr345	UTSW	2	36640189	missense	possibly damaging	0.83
R2207:Olfr345	UTSW	2	36640189	missense	possibly damaging	0.83
R3773:Olfr345	UTSW	2	36640321	missense	probably benign	0.16
R4470:Olfr345	UTSW	2	36640221	missense	probably damaging	1.00
R4583:Olfr345	UTSW	2	36640614	missense	probably damaging	1.00
R4897:Olfr345	UTSW	2	36640894	missense	probably damaging	1.00
R5308:Olfr345	UTSW	2	36640694	nonsense	probably null
R5510:Olfr345	UTSW	2	36640963	missense	probably benign
R6175:Olfr345	UTSW	2	36640051	missense	probably benign	0.00
R7736:Olfr345	UTSW	2	36640185	missense	probably damaging	1.00
R7737:Olfr345	UTSW	2	36640620	missense	probably benign	0.01
R9783:Olfr345	UTSW	2	36640149	missense	probably benign	0.44

Predicted Primers

PCR Primer
(F):5'- ACATGTGGCTCTCACCTCTG -3'
(R):5'- TTTTGTGAGAAACCTACCCAAGATG -3'

Sequencing Primer
(F):5'- GTACTATGGAGCCATCATTGGAC -3'
(R):5'- CCTACCCAAGATGCTGATAAGTAAG -3'

Posted On

2015-11-11