|Institutional Source||Beutler Lab|
|Gene Name||mixed lineage kinase domain-like|
|Essential gene?||Probably non essential (E-score: 0.115)|
|Stock #||R4760 (G1)|
|Chromosomal Location||111311797-111338177 bp(-) (GRCm38)|
|Type of Mutation||critical splice donor site (1 bp from exon)|
|DNA Base Change (assembly)||C to G at 111319716 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000113718 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000056157] [ENSMUST00000120432]|
|Meta Mutation Damage Score||0.9487|
|Coding Region Coverage||
|Validation Efficiency||98% (90/92)|
FUNCTION: This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit imapired macrophage and mouse embryonic fibroblast necroptosis. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Mlkl||
(F):5'- ATGGGATCGCGAGACATTCTAG -3'
(R):5'- TGGGGCTGGATCTTTACAATACC -3'
(F):5'- TGGCGATCTGTGGGCAAC -3'
(R):5'- GCTGGATCTTTACAATACCTTGCTTC -3'