|Institutional Source||Beutler Lab|
|Gene Name||peroxiredoxin 3|
|Synonyms||TDXM, D0Tohi1, Aop1, Prx III, Ef2l, SP22, Mer5|
|Is this an essential gene?||Possibly non essential (E-score: 0.376)|
|Stock #||R4760 (G1)|
|Chromosomal Location||60864051-60874556 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to C at 60873183 bp|
|Amino Acid Change||Cysteine to Tryptophan at position 39 (C39W)|
|Ref Sequence||ENSEMBL: ENSMUSP00000025961 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000025961]|
|Predicted Effect||possibly damaging
AA Change: C39W
PolyPhen 2 Score 0.627 (Sensitivity: 0.87; Specificity: 0.91)
AA Change: C39W
|Meta Mutation Damage Score||0.1795|
|Coding Region Coverage||
|Validation Efficiency||98% (90/92)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. [provided by RefSeq, Oct 2014]
PHENOTYPE: Homozygotes for a null allele show increased fat mass, adipocyte hypertrophy, mitochondrial dysfunction, oxidative stress, adipokine dysregulation and altered lipid and glucose metabolism. Homozygotes for a gene-trap allele show reduced weight and high susceptibility to LPS-induced oxidative stress. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Prdx3||
(F):5'- CCAAGGACTCACTGTTCCTAAC -3'
(R):5'- TCGTTGGGAAAAGGGTCGTTAAC -3'
(F):5'- AACAGTTCCTTTTAGATCCAGCC -3'
(R):5'- TCGTTAACAAAGGATGACAGCAGC -3'