Mutagenetix > Incidental Mutation

Incidental Mutation 'R0403:Usp34'

35717

Institutional Source

Beutler Lab

Gene Symbol

Usp34

Ensembl Gene

ENSMUSG00000056342

Gene Name

ubiquitin specific peptidase 34

Synonyms

Murr2, A530081C03Rik

MMRRC Submission

038608-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.732) question?

Stock #

R0403 (G1)

Quality Score

194

Status

Validated

Chromosome

Chromosomal Location

23306895-23490560 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 23333838 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 177 (H177Q)

Ref Sequence

ENSEMBL: ENSMUSP00000137430 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000180046]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000137823
AA Change: H196Q

SMART Domains

Protein: ENSMUSP00000120747
Gene: ENSMUSG00000056342
AA Change: H196Q

Domain	Start	End	E-Value	Type
low complexity region	489	500	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	591	610	N/A	INTRINSIC
coiled coil region	626	671	N/A	INTRINSIC
low complexity region	827	842	N/A	INTRINSIC
low complexity region	1207	1218	N/A	INTRINSIC
low complexity region	1399	1410	N/A	INTRINSIC
low complexity region	1518	1532	N/A	INTRINSIC
low complexity region	1751	1764	N/A	INTRINSIC
low complexity region	1812	1824	N/A	INTRINSIC
Pfam:UCH	1950	2293	7.6e-44	PFAM
Pfam:UCH_1	1951	2249	3.6e-22	PFAM
low complexity region	2542	2564	N/A	INTRINSIC
low complexity region	2672	2679	N/A	INTRINSIC
Blast:Drf_GBD	2943	3116	3e-53	BLAST
low complexity region	3344	3357	N/A	INTRINSIC
coiled coil region	3371	3393	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000180046
AA Change: H177Q

PolyPhen 2 Score 0.819 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000137430
Gene: ENSMUSG00000056342
AA Change: H177Q

Domain	Start	End	E-Value	Type
low complexity region	469	480	N/A	INTRINSIC
low complexity region	510	524	N/A	INTRINSIC
low complexity region	571	590	N/A	INTRINSIC
coiled coil region	607	652	N/A	INTRINSIC
low complexity region	807	822	N/A	INTRINSIC
low complexity region	1187	1198	N/A	INTRINSIC
low complexity region	1379	1390	N/A	INTRINSIC
low complexity region	1498	1512	N/A	INTRINSIC
low complexity region	1731	1744	N/A	INTRINSIC
low complexity region	1792	1804	N/A	INTRINSIC
Pfam:UCH	1930	2273	2.3e-44	PFAM
Pfam:UCH_1	1931	2229	1.1e-22	PFAM
low complexity region	2522	2544	N/A	INTRINSIC
low complexity region	2652	2659	N/A	INTRINSIC
Blast:Drf_GBD	2923	3096	2e-53	BLAST
low complexity region	3324	3337	N/A	INTRINSIC
coiled coil region	3352	3374	N/A	INTRINSIC

Meta Mutation Damage Score

0.1348

Coding Region Coverage

1x: 98.0%
3x: 96.8%
10x: 93.2%
20x: 83.7%

Validation Efficiency

97% (110/113)

Allele List at MGI

Other mutations in this stock

Total: 105 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ace	A	G	11: 105,973,880 (GRCm38)		probably null	Het
Adgrg3	C	T	8: 95,036,922 (GRCm38)	L284F	probably benign	Het
Alkbh8	T	A	9: 3,385,469 (GRCm38)	V587E	probably damaging	Het
Ap4b1	T	A	3: 103,818,839 (GRCm38)	C244S	probably damaging	Het
Ap4b1	T	A	3: 103,821,396 (GRCm38)	M590K	probably benign	Het
Arhgap15	C	T	2: 44,063,766 (GRCm38)	T168I	probably damaging	Het
Arntl2	T	A	6: 146,822,655 (GRCm38)	H348Q	probably damaging	Het
Atp8b1	A	G	18: 64,540,310 (GRCm38)	V997A	probably damaging	Het
Atrn	G	A	2: 130,906,859 (GRCm38)	C100Y	probably damaging	Het
Baiap2l2	C	A	15: 79,271,216 (GRCm38)	A151S	probably benign	Het
Baz2b	A	T	2: 59,969,377 (GRCm38)	D199E	possibly damaging	Het
Cblb	A	G	16: 52,152,626 (GRCm38)	D440G	probably benign	Het
Cdon	T	C	9: 35,473,500 (GRCm38)	V694A	probably benign	Het
Cep250	A	T	2: 155,992,349 (GRCm38)	R2065W	probably damaging	Het
Ces2b	G	T	8: 104,833,945 (GRCm38)	A131S	probably damaging	Het
Chrna9	A	T	5: 65,967,892 (GRCm38)	T59S	possibly damaging	Het
Cog3	T	A	14: 75,742,327 (GRCm38)		probably benign	Het
Cpa1	G	A	6: 30,641,857 (GRCm38)	V227I	probably benign	Het
Cyp3a25	A	T	5: 145,998,513 (GRCm38)	C98S	probably damaging	Het
D8Ertd738e	C	T	8: 84,249,601 (GRCm38)		probably null	Het
Ddx60	A	G	8: 61,994,541 (GRCm38)		probably benign	Het
Dhx16	T	C	17: 35,883,050 (GRCm38)		probably null	Het
Dnah9	T	A	11: 66,084,789 (GRCm38)	Q1478L	possibly damaging	Het
Dock10	T	C	1: 80,524,070 (GRCm38)	Y1434C	possibly damaging	Het
Enpp3	T	A	10: 24,804,436 (GRCm38)	D325V	probably damaging	Het
Entpd6	C	A	2: 150,760,170 (GRCm38)	T194K	possibly damaging	Het
Fam208b	A	T	13: 3,582,052 (GRCm38)	Y816*	probably null	Het
Fat2	A	T	11: 55,270,349 (GRCm38)	V3185E	probably benign	Het
Flrt1	A	G	19: 7,095,919 (GRCm38)	L421P	probably benign	Het
Fmn2	A	T	1: 174,694,278 (GRCm38)	Q1292L	probably damaging	Het
Fndc1	T	C	17: 7,775,588 (GRCm38)		probably null	Het
Fndc1	T	C	17: 7,753,723 (GRCm38)	D1459G	probably damaging	Het
Fzr1	A	G	10: 81,369,368 (GRCm38)	S265P	possibly damaging	Het
Gm13119	A	G	4: 144,362,646 (GRCm38)	N178S	probably benign	Het
Gm14085	T	C	2: 122,521,854 (GRCm38)	L364S	probably damaging	Het
Gpr142	G	A	11: 114,806,029 (GRCm38)	V134M	probably damaging	Het
Grid2ip	G	T	5: 143,357,620 (GRCm38)	V24L	possibly damaging	Het
Herc2	G	A	7: 56,159,417 (GRCm38)	R2555H	probably damaging	Het
Hpdl	A	T	4: 116,820,479 (GRCm38)	Y262N	possibly damaging	Het
Htr3a	A	G	9: 48,908,659 (GRCm38)	V57A	probably damaging	Het
Igfbp7	T	C	5: 77,355,591 (GRCm38)	I186V	probably benign	Het
Itga2b	C	T	11: 102,467,326 (GRCm38)		probably null	Het
Itgae	A	C	11: 73,123,183 (GRCm38)	D736A	possibly damaging	Het
Itpkc	T	A	7: 27,208,345 (GRCm38)	M645L	probably benign	Het
Jchain	T	C	5: 88,521,378 (GRCm38)	R139G	probably benign	Het
Kif13a	A	G	13: 46,791,401 (GRCm38)	V908A	probably damaging	Het
Kif1b	T	A	4: 149,181,967 (GRCm38)	K389*	probably null	Het
Klhl12	T	C	1: 134,485,856 (GRCm38)	Y360H	possibly damaging	Het
Knop1	G	A	7: 118,853,053 (GRCm38)	R148W	probably damaging	Het
Lpar1	T	A	4: 58,487,191 (GRCm38)	N27Y	probably damaging	Het
Lpar2	T	A	8: 69,824,152 (GRCm38)	V197D	probably damaging	Het
Lrrc74a	A	G	12: 86,740,979 (GRCm38)	N128S	probably damaging	Het
Lum	G	T	10: 97,572,043 (GRCm38)	V337F	probably benign	Het
Mag	T	C	7: 30,906,980 (GRCm38)	D344G	probably damaging	Het
Maip1	G	A	1: 57,407,196 (GRCm38)	A142T	probably benign	Het
Mlh3	A	G	12: 85,268,968 (GRCm38)	V148A	possibly damaging	Het
Nav3	A	G	10: 109,767,103 (GRCm38)	V1195A	probably damaging	Het
Ncor2	A	G	5: 125,033,337 (GRCm38)	S868P	possibly damaging	Het
Nek1	A	G	8: 61,106,855 (GRCm38)	E907G	probably damaging	Het
Nfam1	G	A	15: 83,016,379 (GRCm38)	T134I	probably benign	Het
Nr0b2	T	C	4: 133,553,759 (GRCm38)	V112A	probably damaging	Het
Nrp1	A	G	8: 128,457,969 (GRCm38)	N365S	probably damaging	Het
Nrsn2	T	C	2: 152,369,790 (GRCm38)	Y107C	probably damaging	Het
Ntng1	G	A	3: 109,934,611 (GRCm38)	A282V	probably damaging	Het
Nxf1	T	C	19: 8,765,028 (GRCm38)	I337T	probably damaging	Het
Obscn	C	T	11: 59,076,540 (GRCm38)	G479D	probably damaging	Het
Olfr45	T	C	7: 140,691,309 (GRCm38)	S135P	possibly damaging	Het
Oprd1	T	A	4: 132,113,768 (GRCm38)	D293V	probably benign	Het
P3h2	T	G	16: 25,969,950 (GRCm38)	N586H	possibly damaging	Het
Pcid2	T	C	8: 13,085,367 (GRCm38)	Y214C	probably damaging	Het
Pkd1l3	C	G	8: 109,623,649 (GRCm38)	D375E	possibly damaging	Het
Pkd1l3	G	A	8: 109,623,663 (GRCm38)	S380N	probably benign	Het
Ppic	C	A	18: 53,411,071 (GRCm38)	G81W	probably damaging	Het
Ppp2r1a	C	A	17: 20,957,041 (GRCm38)	P246T	probably damaging	Het
Ppp4r4	T	G	12: 103,584,102 (GRCm38)	S46A	probably benign	Het
Prkce	A	G	17: 86,168,653 (GRCm38)	T21A	probably damaging	Het
Prkg2	T	C	5: 98,994,645 (GRCm38)	E210G	possibly damaging	Het
Prss35	A	G	9: 86,756,037 (GRCm38)	M287V	probably damaging	Het
Psd	G	A	19: 46,320,972 (GRCm38)		probably benign	Het
Ptch2	A	T	4: 117,110,839 (GRCm38)	K843*	probably null	Het
Rab44	C	A	17: 29,145,261 (GRCm38)	T603K	probably damaging	Het
Rasal3	T	A	17: 32,392,790 (GRCm38)		probably null	Het
Rbbp6	A	G	7: 122,992,296 (GRCm38)	T526A	probably damaging	Het
Ros1	A	T	10: 52,143,438 (GRCm38)		probably benign	Het
Sec24b	A	G	3: 129,999,534 (GRCm38)	S685P	probably damaging	Het
Sec24b	T	A	3: 129,989,676 (GRCm38)	L1104F	possibly damaging	Het
Sema6a	G	A	18: 47,290,045 (GRCm38)		probably null	Het
Setmar	A	T	6: 108,075,962 (GRCm38)	H139L	probably benign	Het
Slc4a7	T	C	14: 14,766,808 (GRCm38)	V710A	probably benign	Het
Smarcc1	T	A	9: 110,237,808 (GRCm38)		probably null	Het
Smchd1	G	A	17: 71,394,902 (GRCm38)	L1032F	probably damaging	Het
Speg	T	C	1: 75,430,784 (GRCm38)		probably benign	Het
Tcea1	C	G	1: 4,889,503 (GRCm38)	R134G	probably benign	Het
Tchhl1	C	T	3: 93,471,029 (GRCm38)	Q347*	probably null	Het
Tecrl	A	T	5: 83,354,758 (GRCm38)		probably benign	Het
Tepsin	T	C	11: 120,093,682 (GRCm38)		probably benign	Het
Tmem40	G	A	6: 115,733,985 (GRCm38)		probably benign	Het
Tpr	G	A	1: 150,407,414 (GRCm38)		probably benign	Het
Ttll12	A	T	15: 83,580,658 (GRCm38)		probably benign	Het
Ttn	T	A	2: 76,909,608 (GRCm38)	D3529V	probably benign	Het
Vsig10	T	A	5: 117,338,461 (GRCm38)	S327T	probably benign	Het
Zbtb4	T	A	11: 69,777,639 (GRCm38)	M396K	probably damaging	Het
Zfp352	C	T	4: 90,225,009 (GRCm38)	T462I	possibly damaging	Het
Zfp385b	T	C	2: 77,476,845 (GRCm38)	M145V	probably damaging	Het
Zfp780b	C	A	7: 27,971,689 (GRCm38)	V65F	possibly damaging	Het

Other mutations in Usp34

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00092:Usp34	APN	11	23,436,020 (GRCm38)	missense	probably damaging	0.98
IGL00477:Usp34	APN	11	23,468,879 (GRCm38)	missense	probably damaging	0.99
IGL01307:Usp34	APN	11	23,417,676 (GRCm38)	missense	probably damaging	0.99
IGL01313:Usp34	APN	11	23,473,206 (GRCm38)	missense	probably damaging	1.00
IGL01794:Usp34	APN	11	23,436,020 (GRCm38)	missense	probably damaging	0.98
IGL01826:Usp34	APN	11	23,436,020 (GRCm38)	missense	probably damaging	0.98
IGL01827:Usp34	APN	11	23,436,020 (GRCm38)	missense	probably damaging	0.98
IGL01830:Usp34	APN	11	23,436,020 (GRCm38)	missense	probably damaging	0.98
IGL01867:Usp34	APN	11	23,384,411 (GRCm38)	missense	possibly damaging	0.77
IGL01939:Usp34	APN	11	23,345,141 (GRCm38)	splice site	probably benign
IGL01977:Usp34	APN	11	23,452,661 (GRCm38)	missense	probably damaging	1.00
IGL01985:Usp34	APN	11	23,452,565 (GRCm38)	missense	probably damaging	1.00
IGL02011:Usp34	APN	11	23,471,554 (GRCm38)	missense	probably damaging	0.99
IGL02302:Usp34	APN	11	23,467,243 (GRCm38)	missense	possibly damaging	0.91
IGL02423:Usp34	APN	11	23,354,900 (GRCm38)	missense	probably benign	0.11
IGL02491:Usp34	APN	11	23,432,630 (GRCm38)	missense	probably damaging	0.98
IGL02532:Usp34	APN	11	23,370,291 (GRCm38)	missense	probably damaging	0.99
IGL02561:Usp34	APN	11	23,351,652 (GRCm38)	missense	probably benign	0.09
IGL02706:Usp34	APN	11	23,388,659 (GRCm38)	splice site	probably benign
IGL02891:Usp34	APN	11	23,487,166 (GRCm38)	missense	probably benign	0.09
IGL03079:Usp34	APN	11	23,432,247 (GRCm38)	missense	possibly damaging	0.48
IGL03089:Usp34	APN	11	23,446,958 (GRCm38)	missense	possibly damaging	0.84
IGL03175:Usp34	APN	11	23,488,686 (GRCm38)	missense	probably benign
IGL03256:Usp34	APN	11	23,420,090 (GRCm38)	nonsense	probably null
IGL03280:Usp34	APN	11	23,354,897 (GRCm38)	missense	probably damaging	1.00
IGL03289:Usp34	APN	11	23,393,818 (GRCm38)	missense	possibly damaging	0.94
IGL03408:Usp34	APN	11	23,446,957 (GRCm38)	missense	possibly damaging	0.92
Chub	UTSW	11	23,464,686 (GRCm38)	missense	probably damaging	0.99
Cicione	UTSW	11	23,489,033 (GRCm38)	missense	possibly damaging	0.85
R5571_Usp34_680	UTSW	11	23,457,975 (GRCm38)	missense	probably damaging	0.99
R5713_Usp34_003	UTSW	11	23,343,515 (GRCm38)	missense	possibly damaging	0.94
Roebuck	UTSW	11	23,486,810 (GRCm38)	splice site	probably benign
stoat	UTSW	11	23,487,203 (GRCm38)	missense
tunnelvision	UTSW	11	23,446,968 (GRCm38)	missense
I2288:Usp34	UTSW	11	23,432,473 (GRCm38)	splice site	probably benign
R0047:Usp34	UTSW	11	23,464,403 (GRCm38)	missense	probably benign	0.34
R0047:Usp34	UTSW	11	23,464,403 (GRCm38)	missense	probably benign	0.34
R0099:Usp34	UTSW	11	23,363,111 (GRCm38)	missense	probably damaging	1.00
R0240:Usp34	UTSW	11	23,433,206 (GRCm38)	missense	probably damaging	0.99
R0240:Usp34	UTSW	11	23,433,206 (GRCm38)	missense	probably damaging	0.99
R0432:Usp34	UTSW	11	23,401,505 (GRCm38)	missense	probably damaging	0.99
R0446:Usp34	UTSW	11	23,467,207 (GRCm38)	missense	probably damaging	0.97
R0455:Usp34	UTSW	11	23,446,741 (GRCm38)	splice site	probably benign
R0470:Usp34	UTSW	11	23,436,001 (GRCm38)	missense	possibly damaging	0.94
R0472:Usp34	UTSW	11	23,384,509 (GRCm38)	splice site	probably benign
R0512:Usp34	UTSW	11	23,451,997 (GRCm38)	missense	probably benign	0.04
R0557:Usp34	UTSW	11	23,403,848 (GRCm38)	missense	probably damaging	0.98
R0562:Usp34	UTSW	11	23,432,406 (GRCm38)	splice site	probably benign
R0656:Usp34	UTSW	11	23,472,967 (GRCm38)	missense	probably damaging	0.99
R0693:Usp34	UTSW	11	23,452,637 (GRCm38)	missense	probably damaging	0.97
R0739:Usp34	UTSW	11	23,467,243 (GRCm38)	missense	possibly damaging	0.91
R1061:Usp34	UTSW	11	23,384,420 (GRCm38)	missense	possibly damaging	0.51
R1078:Usp34	UTSW	11	23,433,175 (GRCm38)	splice site	probably benign
R1223:Usp34	UTSW	11	23,446,464 (GRCm38)	splice site	probably null
R1295:Usp34	UTSW	11	23,384,477 (GRCm38)	missense	probably damaging	1.00
R1430:Usp34	UTSW	11	23,459,151 (GRCm38)	missense	probably damaging	0.97
R1445:Usp34	UTSW	11	23,351,629 (GRCm38)	missense	probably damaging	0.99
R1468:Usp34	UTSW	11	23,441,171 (GRCm38)	missense	probably damaging	1.00
R1468:Usp34	UTSW	11	23,441,171 (GRCm38)	missense	probably damaging	1.00
R1471:Usp34	UTSW	11	23,488,862 (GRCm38)	missense	probably benign	0.20
R1475:Usp34	UTSW	11	23,473,253 (GRCm38)	missense	probably damaging	0.99
R1628:Usp34	UTSW	11	23,488,725 (GRCm38)	missense	probably damaging	1.00
R1631:Usp34	UTSW	11	23,460,651 (GRCm38)	missense	probably damaging	0.99
R1655:Usp34	UTSW	11	23,375,051 (GRCm38)	missense	probably benign	0.05
R1741:Usp34	UTSW	11	23,364,103 (GRCm38)	missense	probably benign	0.00
R1854:Usp34	UTSW	11	23,426,153 (GRCm38)	missense	probably benign	0.24
R1867:Usp34	UTSW	11	23,361,593 (GRCm38)	missense	possibly damaging	0.82
R1869:Usp34	UTSW	11	23,364,479 (GRCm38)	missense	probably benign	0.37
R1870:Usp34	UTSW	11	23,364,479 (GRCm38)	missense	probably benign	0.37
R1871:Usp34	UTSW	11	23,364,479 (GRCm38)	missense	probably benign	0.37
R1967:Usp34	UTSW	11	23,364,503 (GRCm38)	missense	probably benign	0.01
R2051:Usp34	UTSW	11	23,464,468 (GRCm38)	missense	probably damaging	0.97
R2132:Usp34	UTSW	11	23,464,556 (GRCm38)	missense	possibly damaging	0.95
R2156:Usp34	UTSW	11	23,382,602 (GRCm38)	missense	probably damaging	0.98
R2205:Usp34	UTSW	11	23,385,147 (GRCm38)	missense	probably damaging	0.97
R2342:Usp34	UTSW	11	23,403,599 (GRCm38)	missense	possibly damaging	0.46
R3431:Usp34	UTSW	11	23,370,466 (GRCm38)	missense	possibly damaging	0.95
R3812:Usp34	UTSW	11	23,464,517 (GRCm38)	missense	possibly damaging	0.94
R3872:Usp34	UTSW	11	23,489,033 (GRCm38)	missense	possibly damaging	0.85
R3873:Usp34	UTSW	11	23,489,033 (GRCm38)	missense	possibly damaging	0.85
R3874:Usp34	UTSW	11	23,489,033 (GRCm38)	missense	possibly damaging	0.85
R3875:Usp34	UTSW	11	23,489,033 (GRCm38)	missense	possibly damaging	0.85
R3925:Usp34	UTSW	11	23,343,640 (GRCm38)	missense	probably benign	0.28
R3972:Usp34	UTSW	11	23,457,803 (GRCm38)	missense	probably damaging	1.00
R4018:Usp34	UTSW	11	23,489,033 (GRCm38)	missense	possibly damaging	0.85
R4042:Usp34	UTSW	11	23,489,033 (GRCm38)	missense	possibly damaging	0.85
R4155:Usp34	UTSW	11	23,417,676 (GRCm38)	missense	probably damaging	0.99
R4197:Usp34	UTSW	11	23,444,189 (GRCm38)	missense	probably damaging	0.98
R4352:Usp34	UTSW	11	23,320,727 (GRCm38)	missense	possibly damaging	0.73
R4379:Usp34	UTSW	11	23,384,499 (GRCm38)	missense	possibly damaging	0.52
R4444:Usp34	UTSW	11	23,435,998 (GRCm38)	missense	probably damaging	0.98
R4475:Usp34	UTSW	11	23,457,975 (GRCm38)	missense	possibly damaging	0.95
R4501:Usp34	UTSW	11	23,401,529 (GRCm38)	missense	probably damaging	1.00
R4527:Usp34	UTSW	11	23,421,257 (GRCm38)	missense	possibly damaging	0.57
R4603:Usp34	UTSW	11	23,464,633 (GRCm38)	missense	probably damaging	0.97
R4612:Usp34	UTSW	11	23,432,268 (GRCm38)	missense	probably damaging	0.99
R4673:Usp34	UTSW	11	23,364,480 (GRCm38)	small deletion	probably benign
R4707:Usp34	UTSW	11	23,487,215 (GRCm38)	missense	probably damaging	1.00
R4736:Usp34	UTSW	11	23,393,749 (GRCm38)	splice site	probably null
R4867:Usp34	UTSW	11	23,451,999 (GRCm38)	missense	probably benign	0.28
R4879:Usp34	UTSW	11	23,373,410 (GRCm38)	missense	possibly damaging	0.94
R4977:Usp34	UTSW	11	23,488,982 (GRCm38)	missense	probably damaging	1.00
R5004:Usp34	UTSW	11	23,464,586 (GRCm38)	missense	probably damaging	1.00
R5057:Usp34	UTSW	11	23,458,086 (GRCm38)	intron	probably benign
R5068:Usp34	UTSW	11	23,460,665 (GRCm38)	missense	possibly damaging	0.94
R5304:Usp34	UTSW	11	23,343,616 (GRCm38)	missense	probably damaging	1.00
R5320:Usp34	UTSW	11	23,333,739 (GRCm38)	missense	probably benign
R5327:Usp34	UTSW	11	23,468,846 (GRCm38)	missense	probably damaging	1.00
R5328:Usp34	UTSW	11	23,488,659 (GRCm38)	missense	probably benign	0.04
R5328:Usp34	UTSW	11	23,464,616 (GRCm38)	missense	probably benign	0.01
R5390:Usp34	UTSW	11	23,444,202 (GRCm38)	critical splice donor site	probably null
R5434:Usp34	UTSW	11	23,412,271 (GRCm38)	missense	probably damaging	0.99
R5523:Usp34	UTSW	11	23,349,198 (GRCm38)	missense	probably benign	0.39
R5567:Usp34	UTSW	11	23,488,336 (GRCm38)	missense	probably damaging	0.97
R5571:Usp34	UTSW	11	23,457,975 (GRCm38)	missense	probably damaging	0.99
R5645:Usp34	UTSW	11	23,375,024 (GRCm38)	missense	possibly damaging	0.86
R5713:Usp34	UTSW	11	23,343,515 (GRCm38)	missense	possibly damaging	0.94
R5719:Usp34	UTSW	11	23,354,846 (GRCm38)	missense	probably benign	0.00
R5813:Usp34	UTSW	11	23,421,340 (GRCm38)	missense	probably benign	0.38
R5921:Usp34	UTSW	11	23,464,686 (GRCm38)	missense	probably damaging	0.99
R5928:Usp34	UTSW	11	23,436,040 (GRCm38)	missense	probably damaging	0.98
R5944:Usp34	UTSW	11	23,363,089 (GRCm38)	missense	probably damaging	1.00
R6198:Usp34	UTSW	11	23,484,127 (GRCm38)	missense	probably damaging	1.00
R6229:Usp34	UTSW	11	23,446,778 (GRCm38)	missense	probably damaging	0.99
R6306:Usp34	UTSW	11	23,412,260 (GRCm38)	missense	possibly damaging	0.94
R6320:Usp34	UTSW	11	23,452,520 (GRCm38)	missense	probably damaging	0.98
R6341:Usp34	UTSW	11	23,381,353 (GRCm38)	missense	probably damaging	0.97
R6374:Usp34	UTSW	11	23,438,914 (GRCm38)	missense	probably damaging	1.00
R6398:Usp34	UTSW	11	23,488,666 (GRCm38)	missense	probably benign
R6438:Usp34	UTSW	11	23,364,266 (GRCm38)	missense	probably benign	0.02
R6668:Usp34	UTSW	11	23,460,659 (GRCm38)	missense	probably damaging	0.97
R6700:Usp34	UTSW	11	23,439,011 (GRCm38)	missense	probably damaging	1.00
R6783:Usp34	UTSW	11	23,412,318 (GRCm38)	missense	probably damaging	1.00
R6821:Usp34	UTSW	11	23,367,491 (GRCm38)	missense	possibly damaging	0.79
R6855:Usp34	UTSW	11	23,452,569 (GRCm38)	missense	possibly damaging	0.94
R6916:Usp34	UTSW	11	23,458,023 (GRCm38)	missense	probably damaging	0.98
R7020:Usp34	UTSW	11	23,393,954 (GRCm38)	missense	probably benign	0.05
R7026:Usp34	UTSW	11	23,361,622 (GRCm38)	missense	probably damaging	1.00
R7085:Usp34	UTSW	11	23,363,097 (GRCm38)	missense
R7101:Usp34	UTSW	11	23,426,183 (GRCm38)	missense
R7168:Usp34	UTSW	11	23,464,585 (GRCm38)	missense
R7192:Usp34	UTSW	11	23,460,571 (GRCm38)	missense
R7264:Usp34	UTSW	11	23,333,566 (GRCm38)	missense	probably benign	0.00
R7325:Usp34	UTSW	11	23,419,052 (GRCm38)	missense
R7343:Usp34	UTSW	11	23,488,868 (GRCm38)	missense
R7358:Usp34	UTSW	11	23,361,683 (GRCm38)	missense	probably damaging	0.99
R7369:Usp34	UTSW	11	23,432,361 (GRCm38)	missense
R7389:Usp34	UTSW	11	23,345,200 (GRCm38)	missense
R7459:Usp34	UTSW	11	23,364,458 (GRCm38)	missense	possibly damaging	0.53
R7517:Usp34	UTSW	11	23,446,968 (GRCm38)	missense
R7729:Usp34	UTSW	11	23,449,268 (GRCm38)	missense
R7777:Usp34	UTSW	11	23,382,638 (GRCm38)	missense
R7810:Usp34	UTSW	11	23,412,314 (GRCm38)	missense
R7836:Usp34	UTSW	11	23,446,614 (GRCm38)	missense
R7862:Usp34	UTSW	11	23,464,718 (GRCm38)	missense
R7993:Usp34	UTSW	11	23,377,622 (GRCm38)	missense
R8050:Usp34	UTSW	11	23,446,787 (GRCm38)	missense
R8054:Usp34	UTSW	11	23,361,295 (GRCm38)	missense
R8239:Usp34	UTSW	11	23,446,750 (GRCm38)	missense
R8266:Usp34	UTSW	11	23,486,810 (GRCm38)	splice site	probably benign
R8347:Usp34	UTSW	11	23,412,345 (GRCm38)	missense
R8409:Usp34	UTSW	11	23,457,811 (GRCm38)	missense
R8692:Usp34	UTSW	11	23,429,325 (GRCm38)	missense
R8694:Usp34	UTSW	11	23,484,161 (GRCm38)	missense
R8734:Usp34	UTSW	11	23,444,184 (GRCm38)	missense
R8806:Usp34	UTSW	11	23,484,143 (GRCm38)	missense
R8914:Usp34	UTSW	11	23,343,604 (GRCm38)	missense
R8987:Usp34	UTSW	11	23,464,267 (GRCm38)	missense
R9013:Usp34	UTSW	11	23,370,302 (GRCm38)	missense
R9108:Usp34	UTSW	11	23,370,528 (GRCm38)	missense
R9264:Usp34	UTSW	11	23,489,064 (GRCm38)	missense
R9301:Usp34	UTSW	11	23,472,951 (GRCm38)	missense
R9375:Usp34	UTSW	11	23,487,203 (GRCm38)	missense
R9385:Usp34	UTSW	11	23,449,223 (GRCm38)	missense
R9500:Usp34	UTSW	11	23,381,337 (GRCm38)	missense	probably damaging	0.99
R9566:Usp34	UTSW	11	23,367,529 (GRCm38)	missense
R9629:Usp34	UTSW	11	23,364,364 (GRCm38)	missense
R9679:Usp34	UTSW	11	23,444,369 (GRCm38)	missense
R9680:Usp34	UTSW	11	23,367,385 (GRCm38)	missense	possibly damaging	0.94
R9686:Usp34	UTSW	11	23,474,351 (GRCm38)	missense
R9752:Usp34	UTSW	11	23,459,182 (GRCm38)	missense	probably benign	0.11
X0023:Usp34	UTSW	11	23,375,028 (GRCm38)	missense	possibly damaging	0.73
X0057:Usp34	UTSW	11	23,457,824 (GRCm38)	missense	possibly damaging	0.86
Z1176:Usp34	UTSW	11	23,473,221 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCCTAGCAGGAAGGGGAAAATTGATG -3'
(R):5'- agaaagaaGAAAGAAGGGAATGGCAGAC -3'

Sequencing Primer
(F):5'- CGGGACCAGCTTTGTAAACATTG -3'
(R):5'- TGGCAGACAGACAGGGTAG -3'

Posted On

2013-05-09