Mutagenetix > Incidental Mutation

Incidental Mutation 'R4751:Muc5ac'

357599

Institutional Source

Beutler Lab

Gene Symbol

Muc5ac

Ensembl Gene

ENSMUSG00000037974

Gene Name

mucin 5, subtypes A and C, tracheobronchial/gastric

Synonyms

MGM, 2210005L13Rik

MMRRC Submission

041970-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4751 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

141788972-141819231 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 141817601 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 3285 (F3285L)

Ref Sequence

ENSEMBL: ENSMUSP00000122353 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000041924] [ENSMUST00000155534] [ENSMUST00000163321]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000041924
AA Change: F2581L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000039699
Gene: ENSMUSG00000037974
AA Change: F2581L

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
VWD	69	226	4.19e-30	SMART
C8	258	334	1.34e-11	SMART
Pfam:TIL	337	393	1.6e-14	PFAM
VWC	395	462	8.6e-18	SMART
VWD	421	585	1.55e-33	SMART
C8	622	696	8.42e-36	SMART
Pfam:TIL	702	759	6.1e-9	PFAM
VWC	761	825	6.75e-1	SMART
VWC	863	905	4.06e-1	SMART
VWD	890	1050	1.51e-45	SMART
C8	1086	1160	2.78e-36	SMART
low complexity region	1315	1330	N/A	INTRINSIC
low complexity region	1333	1366	N/A	INTRINSIC
low complexity region	1371	1387	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1394	1482	2.3e-25	PFAM
low complexity region	1521	1532	N/A	INTRINSIC
low complexity region	1536	1563	N/A	INTRINSIC
low complexity region	1578	1595	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1604	1692	2.2e-27	PFAM
Pfam:Mucin2_WxxW	1765	1857	8.6e-27	PFAM
low complexity region	1875	1895	N/A	INTRINSIC
low complexity region	1949	1968	N/A	INTRINSIC
VWD	2030	2199	4.17e-48	SMART
C8	2242	2311	3.95e-9	SMART
VWC	2376	2439	1.04e-11	SMART
VWC	2479	2543	9.31e-5	SMART
CT	2625	2711	3.43e-32	SMART
low complexity region	2720	2733	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000155534
AA Change: F3285L

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000122353
Gene: ENSMUSG00000037974
AA Change: F3285L

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
VWD	69	226	4.19e-30	SMART
C8	258	334	1.34e-11	SMART
Pfam:TIL	337	393	9.6e-15	PFAM
VWC	395	437	3.54e-1	SMART
VWD	422	586	2.35e-33	SMART
C8	623	697	8.42e-36	SMART
Pfam:TIL	703	760	3.6e-9	PFAM
VWC	762	826	6.75e-1	SMART
VWC	864	906	4.06e-1	SMART
VWD	891	1051	1.51e-45	SMART
C8	1087	1161	2.78e-36	SMART
low complexity region	1316	1331	N/A	INTRINSIC
low complexity region	1334	1367	N/A	INTRINSIC
low complexity region	1372	1388	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1395	1483	1.3e-25	PFAM
low complexity region	1522	1533	N/A	INTRINSIC
low complexity region	1537	1564	N/A	INTRINSIC
low complexity region	1579	1596	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1605	1693	1.3e-27	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000163321
AA Change: F2581L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000131681
Gene: ENSMUSG00000037974
AA Change: F2581L

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
VWD	69	226	4.19e-30	SMART
C8	258	334	1.34e-11	SMART
Pfam:TIL	337	393	7.9e-15	PFAM
VWC	395	462	8.6e-18	SMART
VWD	421	585	1.55e-33	SMART
C8	622	696	8.42e-36	SMART
Pfam:TIL	702	759	1.9e-9	PFAM
VWC	761	825	6.75e-1	SMART
VWC	863	905	4.06e-1	SMART
VWD	890	1050	1.51e-45	SMART
C8	1086	1160	2.78e-36	SMART
low complexity region	1315	1330	N/A	INTRINSIC
low complexity region	1333	1366	N/A	INTRINSIC
low complexity region	1371	1387	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1394	1481	1.1e-23	PFAM
low complexity region	1521	1532	N/A	INTRINSIC
low complexity region	1536	1563	N/A	INTRINSIC
low complexity region	1578	1595	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1604	1691	1.1e-25	PFAM
Pfam:Mucin2_WxxW	1765	1856	6.3e-24	PFAM
low complexity region	1875	1895	N/A	INTRINSIC
low complexity region	1949	1968	N/A	INTRINSIC
VWD	2030	2199	4.17e-48	SMART
C8	2242	2311	3.95e-9	SMART
VWC	2376	2439	1.04e-11	SMART
VWC	2479	2543	9.31e-5	SMART
CT	2625	2711	3.43e-32	SMART
low complexity region	2720	2733	N/A	INTRINSIC

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.4%

Validation Efficiency

95% (124/131)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased susceptibility to T. muris infection with persistent worm burden, goblet cell hyperplasia, and increased serum IFN-gamma despite a normal TH2-type immune response. A portion of mice show corneal opacity and poor tear quality. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 115 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130023H24Rik	A	T	7: 128,237,086 (GRCm38)	C112S	probably benign	Het
Abca13	G	A	11: 9,277,973 (GRCm38)		probably null	Het
Abca14	A	G	7: 120,312,177 (GRCm38)	E1328G	probably benign	Het
Abca9	T	A	11: 110,130,570 (GRCm38)	I1105F	probably benign	Het
Abr	T	C	11: 76,456,608 (GRCm38)	N396D	possibly damaging	Het
Aftph	A	C	11: 20,727,074 (GRCm38)	C178W	probably damaging	Het
Akr1c14	T	C	13: 4,065,338 (GRCm38)	F89S	possibly damaging	Het
Ank3	C	T	10: 69,986,206 (GRCm38)	A1518V	probably benign	Het
Arfgap2	T	G	2: 91,267,368 (GRCm38)	S143R	probably benign	Het
Aspdh	T	A	7: 44,467,205 (GRCm38)	C107*	probably null	Het
Asphd2	C	A	5: 112,391,746 (GRCm38)	G74W	probably damaging	Het
AU040320	G	A	4: 126,854,466 (GRCm38)		probably null	Het
B3glct	T	A	5: 149,725,402 (GRCm38)		probably null	Het
Bcl9l	A	G	9: 44,506,803 (GRCm38)	K646R	probably damaging	Het
Brat1	T	G	5: 140,718,296 (GRCm38)	L768R	probably damaging	Het
Btbd18	T	A	2: 84,667,921 (GRCm38)	Y634*	probably null	Het
Bub1	T	A	2: 127,823,938 (GRCm38)		probably benign	Het
Carns1	A	T	19: 4,166,418 (GRCm38)	D588E	probably damaging	Het
Cast	T	C	13: 74,746,047 (GRCm38)	K141E	probably damaging	Het
Cd109	CATTTATTTATTTATTTATTTATTTATTTATTTAT	CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT	9: 78,712,500 (GRCm38)		probably benign	Het
Chsy3	T	C	18: 59,175,800 (GRCm38)	S42P	possibly damaging	Het
Clca1	A	T	3: 145,004,848 (GRCm38)	F865I	possibly damaging	Het
Clec4f	T	A	6: 83,645,282 (GRCm38)	M526L	possibly damaging	Het
Clnk	T	C	5: 38,720,913 (GRCm38)	E301G	probably benign	Het
Colgalt2	T	A	1: 152,489,876 (GRCm38)	I309N	probably benign	Het
Cyp2e1	A	T	7: 140,774,716 (GRCm38)	K326*	probably null	Het
Dagla	T	A	19: 10,250,394 (GRCm38)	T717S	probably benign	Het
Dnajc11	G	A	4: 151,968,542 (GRCm38)	R141H	probably benign	Het
Dst	A	G	1: 34,191,884 (GRCm38)	K2853E	probably benign	Het
Eif4g1	A	G	16: 20,686,515 (GRCm38)	K1208E	possibly damaging	Het
Elane	A	G	10: 79,886,791 (GRCm38)	R48G	probably benign	Het
Fbxo30	T	A	10: 11,290,195 (GRCm38)	N220K	probably benign	Het
Fbxo33	A	T	12: 59,200,928 (GRCm38)		probably benign	Het
Fetub	G	A	16: 22,937,895 (GRCm38)	V169I	probably benign	Het
Gm13762	A	G	2: 88,973,133 (GRCm38)	C253R	probably damaging	Het
Gm5861	T	A	5: 11,186,491 (GRCm38)	Y138N	probably damaging	Het
Gpx6	A	C	13: 21,317,064 (GRCm38)	Q107H	probably damaging	Het
Hist2h2bb	A	T	3: 96,269,151 (GRCm38)		probably benign	Het
Homer3	A	T	8: 70,285,434 (GRCm38)	I19F	probably damaging	Het
Hspa4	C	T	11: 53,284,199 (GRCm38)	V144I	probably benign	Het
Icos	C	T	1: 60,993,717 (GRCm38)	S25L	probably benign	Het
Ifna4	A	G	4: 88,841,948 (GRCm38)	T30A	probably benign	Het
Ino80b	C	T	6: 83,124,750 (GRCm38)	G46D	probably damaging	Het
Kpna2	T	G	11: 106,992,664 (GRCm38)	I100L	possibly damaging	Het
Krt71	C	T	15: 101,735,466 (GRCm38)	G446R	probably damaging	Het
Krtap31-2	A	G	11: 99,936,576 (GRCm38)	N78S	possibly damaging	Het
Lman1	A	G	18: 65,998,434 (GRCm38)	S132P	probably benign	Het
Lmna	T	C	3: 88,486,533 (GRCm38)	Q246R	possibly damaging	Het
Lrp10	T	A	14: 54,468,592 (GRCm38)	V413E	probably damaging	Het
Macf1	A	C	4: 123,471,650 (GRCm38)	I1541S	probably benign	Het
Med24	A	G	11: 98,706,432 (GRCm38)	L874P	probably damaging	Het
Mgll	T	A	6: 88,725,111 (GRCm38)		probably benign	Het
Mrpl37	A	T	4: 107,057,475 (GRCm38)	L364Q	probably damaging	Het
Mrpl47	C	T	3: 32,728,441 (GRCm38)	R209H	probably benign	Het
Mylk	A	G	16: 34,879,169 (GRCm38)	R301G	probably benign	Het
Nacad	A	G	11: 6,605,726 (GRCm38)	L8P	unknown	Het
Ncf1	T	A	5: 134,229,545 (GRCm38)	H8L	probably damaging	Het
Ncoa3	T	A	2: 166,069,903 (GRCm38)	M1383K	possibly damaging	Het
Necab2	C	T	8: 119,467,598 (GRCm38)	S271L	probably benign	Het
Nme8	A	T	13: 19,675,638 (GRCm38)		probably null	Het
Nsrp1	T	G	11: 77,076,719 (GRCm38)	T16P	possibly damaging	Het
Obox3	A	T	7: 15,625,692 (GRCm38)		probably null	Het
Olfr1211	T	A	2: 88,929,914 (GRCm38)	I134F	probably damaging	Het
Olfr1416	C	A	1: 92,479,983 (GRCm38)	A213S	probably benign	Het
Olfr1428	T	A	19: 12,109,177 (GRCm38)	Y123F	probably damaging	Het
Olfr355	T	C	2: 36,927,583 (GRCm38)	H177R	probably damaging	Het
Olfr667	A	T	7: 104,916,410 (GRCm38)	Y295*	probably null	Het
Osbpl3	C	G	6: 50,300,997 (GRCm38)	E790Q	possibly damaging	Het
Osmr	A	T	15: 6,842,852 (GRCm38)	W254R	probably damaging	Het
Otoa	T	C	7: 121,132,924 (GRCm38)		probably benign	Het
Pagr1a	A	T	7: 127,015,379 (GRCm38)	L218H	probably damaging	Het
Pcdha11	G	A	18: 37,006,944 (GRCm38)	G542D	probably damaging	Het
Pck2	T	A	14: 55,542,561 (GRCm38)	I54N	probably damaging	Het
Ppif	T	C	14: 25,699,499 (GRCm38)	V173A	probably damaging	Het
Ptgs2	T	A	1: 150,104,020 (GRCm38)	L292H	probably damaging	Het
Ptpru	A	G	4: 131,802,586 (GRCm38)	S604P	probably damaging	Het
Qrfpr	T	C	3: 36,182,622 (GRCm38)	H210R	possibly damaging	Het
Rasgrf1	A	G	9: 90,012,866 (GRCm38)	H1113R	probably damaging	Het
Rasgrf1	A	G	9: 89,910,118 (GRCm38)	T41A	probably damaging	Het
Rhoc	T	A	3: 104,792,647 (GRCm38)	I80N	probably damaging	Het
Riok3	A	G	18: 12,153,983 (GRCm38)	N472S	probably benign	Het
Rnf213	A	G	11: 119,445,745 (GRCm38)	Y3314C	probably benign	Het
Shank2	C	T	7: 144,409,468 (GRCm38)	T264I	probably damaging	Het
Shroom3	G	T	5: 92,943,086 (GRCm38)	V1151F	probably damaging	Het
Sipa1l1	A	G	12: 82,341,194 (GRCm38)	I65V	probably benign	Het
Slc22a19	T	C	19: 7,691,145 (GRCm38)	K291R	possibly damaging	Het
Slc22a20	T	C	19: 5,980,460 (GRCm38)	I315V	probably benign	Het
Slc44a1	A	T	4: 53,560,973 (GRCm38)	D563V	probably damaging	Het
Smchd1	A	T	17: 71,391,468 (GRCm38)	H1104Q	probably benign	Het
Spcs2	A	T	7: 99,844,769 (GRCm38)		probably null	Het
Sptb	T	C	12: 76,627,110 (GRCm38)	E301G	probably benign	Het
Stum	T	C	1: 180,442,669 (GRCm38)	D86G	probably damaging	Het
Sufu	A	T	19: 46,483,649 (GRCm38)	D449V	probably benign	Het
Sun5	T	C	2: 153,866,016 (GRCm38)		probably null	Het
Tbc1d15	T	A	10: 115,202,587 (GRCm38)	I574F	probably damaging	Het
Tert	G	A	13: 73,628,063 (GRCm38)	S311N	possibly damaging	Het
Tiparp	T	C	3: 65,552,804 (GRCm38)	Y507H	probably damaging	Het
Tnk2	T	C	16: 32,679,857 (GRCm38)	C158R	probably damaging	Het
Tpgs1	T	C	10: 79,675,620 (GRCm38)	S199P	possibly damaging	Het
Trdmt1	G	T	2: 13,544,653 (GRCm38)		probably benign	Het
Trp53bp1	T	A	2: 121,227,809 (GRCm38)	D944V	probably damaging	Het
Tsc1	T	A	2: 28,679,081 (GRCm38)	I720N	probably damaging	Het
Ttn	T	C	2: 76,897,581 (GRCm38)		probably benign	Het
Ttn	T	C	2: 76,709,605 (GRCm38)	T34346A	probably benign	Het
Uba7	T	A	9: 107,979,805 (GRCm38)	C686S	possibly damaging	Het
Ush2a	T	A	1: 188,850,087 (GRCm38)	F3782L	probably damaging	Het
Vars2	A	T	17: 35,659,343 (GRCm38)	H745Q	possibly damaging	Het
Vgf	T	A	5: 137,032,401 (GRCm38)	D472E	probably damaging	Het
Vmn2r15	T	C	5: 109,286,754 (GRCm38)	M695V	probably benign	Het
Vps41	A	T	13: 18,811,622 (GRCm38)	D208V	probably damaging	Het
Vta1	C	T	10: 14,655,816 (GRCm38)	A272T	probably benign	Het
Wnk4	G	A	11: 101,276,362 (GRCm38)		probably benign	Het
Zfp507	T	C	7: 35,794,382 (GRCm38)	K412R	probably damaging	Het
Zfp768	A	G	7: 127,344,762 (GRCm38)	F65L	possibly damaging	Het
Zfp956	T	C	6: 47,963,576 (GRCm38)	S290P	probably benign	Het

Other mutations in Muc5ac

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00899:Muc5ac	APN	7	141,812,703 (GRCm38)	missense	possibly damaging	0.93
IGL01064:Muc5ac	APN	7	141,807,473 (GRCm38)	missense	probably benign	0.12
IGL01155:Muc5ac	APN	7	141,806,943 (GRCm38)	splice site	probably benign
IGL01452:Muc5ac	APN	7	141,817,555 (GRCm38)	missense	probably benign	0.00
IGL01590:Muc5ac	APN	7	141,798,893 (GRCm38)	missense	probably benign	0.02
IGL02104:Muc5ac	APN	7	141,811,078 (GRCm38)	missense	probably damaging	0.98
IGL02152:Muc5ac	APN	7	141,800,177 (GRCm38)	missense	possibly damaging	0.86
IGL02153:Muc5ac	APN	7	141,818,800 (GRCm38)	nonsense	probably null
IGL02178:Muc5ac	APN	7	141,805,447 (GRCm38)	splice site	probably benign
IGL02403:Muc5ac	APN	7	141,803,450 (GRCm38)	missense	possibly damaging	0.71
IGL02576:Muc5ac	APN	7	141,817,044 (GRCm38)	missense	probably benign	0.01
IGL02665:Muc5ac	APN	7	141,791,086 (GRCm38)	missense	possibly damaging	0.71
IGL02704:Muc5ac	APN	7	141,795,263 (GRCm38)	missense	possibly damaging	0.71
IGL02808:Muc5ac	APN	7	141,805,775 (GRCm38)	missense	possibly damaging	0.72
IGL03283:Muc5ac	APN	7	141,813,781 (GRCm38)	missense	probably benign	0.34
IGL03384:Muc5ac	APN	7	141,812,403 (GRCm38)	missense	possibly damaging	0.71
IGL03046:Muc5ac	UTSW	7	141,795,213 (GRCm38)	missense	probably benign	0.27
PIT4515001:Muc5ac	UTSW	7	141,807,416 (GRCm38)	missense	probably damaging	0.99
R0092:Muc5ac	UTSW	7	141,818,630 (GRCm38)	missense	possibly damaging	0.72
R0145:Muc5ac	UTSW	7	141,795,275 (GRCm38)	missense	possibly damaging	0.71
R0147:Muc5ac	UTSW	7	141,811,039 (GRCm38)	missense	probably benign	0.08
R0363:Muc5ac	UTSW	7	141,800,960 (GRCm38)	missense	probably benign	0.01
R0384:Muc5ac	UTSW	7	141,812,251 (GRCm38)	missense	possibly damaging	0.71
R0440:Muc5ac	UTSW	7	141,792,034 (GRCm38)	nonsense	probably null
R0583:Muc5ac	UTSW	7	141,807,608 (GRCm38)	missense	probably damaging	0.99
R0616:Muc5ac	UTSW	7	141,796,244 (GRCm38)	missense	probably benign	0.02
R0682:Muc5ac	UTSW	7	141,805,669 (GRCm38)	missense	possibly damaging	0.53
R0685:Muc5ac	UTSW	7	141,807,709 (GRCm38)	missense	probably benign	0.03
R0883:Muc5ac	UTSW	7	141,796,265 (GRCm38)	missense	possibly damaging	0.71
R0924:Muc5ac	UTSW	7	141,807,515 (GRCm38)	missense	possibly damaging	0.68
R1300:Muc5ac	UTSW	7	141,816,929 (GRCm38)	missense	possibly damaging	0.73
R1315:Muc5ac	UTSW	7	141,807,323 (GRCm38)	missense	probably damaging	0.99
R1354:Muc5ac	UTSW	7	141,807,377 (GRCm38)	missense	probably damaging	0.99
R1484:Muc5ac	UTSW	7	141,813,892 (GRCm38)	splice site	probably null
R1599:Muc5ac	UTSW	7	141,798,903 (GRCm38)	missense	possibly damaging	0.52
R1758:Muc5ac	UTSW	7	141,801,531 (GRCm38)	missense	possibly damaging	0.86
R1837:Muc5ac	UTSW	7	141,807,086 (GRCm38)	missense	probably benign	0.00
R1911:Muc5ac	UTSW	7	141,796,304 (GRCm38)	missense	probably benign	0.18
R1922:Muc5ac	UTSW	7	141,793,689 (GRCm38)	missense	probably benign	0.03
R1966:Muc5ac	UTSW	7	141,803,376 (GRCm38)	missense	possibly damaging	0.92
R1994:Muc5ac	UTSW	7	141,813,152 (GRCm38)	missense	possibly damaging	0.93
R2056:Muc5ac	UTSW	7	141,792,035 (GRCm38)	missense	probably benign	0.01
R2126:Muc5ac	UTSW	7	141,810,742 (GRCm38)	missense	possibly damaging	0.84
R2170:Muc5ac	UTSW	7	141,812,347 (GRCm38)	missense	possibly damaging	0.93
R2258:Muc5ac	UTSW	7	141,791,008 (GRCm38)	missense	probably benign	0.41
R2259:Muc5ac	UTSW	7	141,791,008 (GRCm38)	missense	probably benign	0.41
R2293:Muc5ac	UTSW	7	141,807,199 (GRCm38)	missense	probably damaging	0.99
R2435:Muc5ac	UTSW	7	141,818,104 (GRCm38)	missense	possibly damaging	0.53
R2895:Muc5ac	UTSW	7	141,791,140 (GRCm38)	missense	possibly damaging	0.92
R2910:Muc5ac	UTSW	7	141,807,641 (GRCm38)	missense	probably damaging	0.99
R3154:Muc5ac	UTSW	7	141,792,736 (GRCm38)	splice site	probably null
R3762:Muc5ac	UTSW	7	141,807,475 (GRCm38)	missense	possibly damaging	0.53
R3791:Muc5ac	UTSW	7	141,798,501 (GRCm38)	missense	probably benign	0.32
R3806:Muc5ac	UTSW	7	141,813,734 (GRCm38)	missense	possibly damaging	0.91
R3825:Muc5ac	UTSW	7	141,814,723 (GRCm38)	missense	possibly damaging	0.92
R3888:Muc5ac	UTSW	7	141,791,224 (GRCm38)	missense	possibly damaging	0.51
R3929:Muc5ac	UTSW	7	141,802,892 (GRCm38)	missense	probably benign
R3981:Muc5ac	UTSW	7	141,813,775 (GRCm38)	missense	possibly damaging	0.86
R4034:Muc5ac	UTSW	7	141,799,844 (GRCm38)	critical splice donor site	probably null
R4043:Muc5ac	UTSW	7	141,807,478 (GRCm38)	missense	possibly damaging	0.53
R4061:Muc5ac	UTSW	7	141,811,130 (GRCm38)	missense	possibly damaging	0.85
R4106:Muc5ac	UTSW	7	141,802,835 (GRCm38)	missense	possibly damaging	0.86
R4206:Muc5ac	UTSW	7	141,817,110 (GRCm38)	missense	possibly damaging	0.73
R4613:Muc5ac	UTSW	7	141,791,103 (GRCm38)	missense	possibly damaging	0.93
R4719:Muc5ac	UTSW	7	141,789,763 (GRCm38)	missense	possibly damaging	0.83
R4789:Muc5ac	UTSW	7	141,798,882 (GRCm38)	missense	possibly damaging	0.86
R4928:Muc5ac	UTSW	7	141,817,902 (GRCm38)	nonsense	probably null
R4971:Muc5ac	UTSW	7	141,816,278 (GRCm38)	missense	possibly damaging	0.68
R4982:Muc5ac	UTSW	7	141,809,456 (GRCm38)	intron	probably benign
R5088:Muc5ac	UTSW	7	141,796,319 (GRCm38)	missense	possibly damaging	0.53
R5141:Muc5ac	UTSW	7	141,814,742 (GRCm38)	missense	possibly damaging	0.72
R5224:Muc5ac	UTSW	7	141,793,971 (GRCm38)	missense	probably benign	0.32
R5366:Muc5ac	UTSW	7	141,807,550 (GRCm38)	missense	probably benign	0.01
R5497:Muc5ac	UTSW	7	141,807,643 (GRCm38)	missense	probably damaging	0.99
R5507:Muc5ac	UTSW	7	141,807,832 (GRCm38)	missense	possibly damaging	0.72
R5643:Muc5ac	UTSW	7	141,793,715 (GRCm38)	critical splice donor site	probably null
R5811:Muc5ac	UTSW	7	141,798,984 (GRCm38)	missense	possibly damaging	0.51
R5946:Muc5ac	UTSW	7	141,817,907 (GRCm38)	missense	possibly damaging	0.73
R5970:Muc5ac	UTSW	7	141,790,669 (GRCm38)	nonsense	probably null
R5977:Muc5ac	UTSW	7	141,796,367 (GRCm38)	missense	possibly damaging	0.73
R6051:Muc5ac	UTSW	7	141,811,857 (GRCm38)	missense	possibly damaging	0.53
R6126:Muc5ac	UTSW	7	141,801,232 (GRCm38)	missense	possibly damaging	0.71
R6159:Muc5ac	UTSW	7	141,815,586 (GRCm38)	missense	possibly damaging	0.53
R6256:Muc5ac	UTSW	7	141,789,795 (GRCm38)	missense	possibly damaging	0.53
R6283:Muc5ac	UTSW	7	141,816,864 (GRCm38)	nonsense	probably null
R6341:Muc5ac	UTSW	7	141,801,492 (GRCm38)	missense	probably damaging	0.99
R6356:Muc5ac	UTSW	7	141,812,679 (GRCm38)	missense	probably benign	0.05
R6481:Muc5ac	UTSW	7	141,809,071 (GRCm38)	intron	probably benign
R6483:Muc5ac	UTSW	7	141,802,854 (GRCm38)	missense	probably benign	0.18
R6627:Muc5ac	UTSW	7	141,808,690 (GRCm38)	intron	probably benign
R6636:Muc5ac	UTSW	7	141,818,605 (GRCm38)	missense	possibly damaging	0.86
R6637:Muc5ac	UTSW	7	141,818,605 (GRCm38)	missense	possibly damaging	0.86
R6656:Muc5ac	UTSW	7	141,803,328 (GRCm38)	missense	probably damaging	0.98
R6721:Muc5ac	UTSW	7	141,798,992 (GRCm38)	missense	possibly damaging	0.71
R6794:Muc5ac	UTSW	7	141,809,552 (GRCm38)	intron	probably benign
R6844:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6847:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6852:Muc5ac	UTSW	7	141,816,907 (GRCm38)	missense	probably benign	0.03
R6862:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6863:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6864:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6865:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6874:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6875:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6876:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6877:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6889:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R6920:Muc5ac	UTSW	7	141,793,298 (GRCm38)	missense	possibly damaging	0.86
R6998:Muc5ac	UTSW	7	141,818,714 (GRCm38)	missense	possibly damaging	0.92
R7017:Muc5ac	UTSW	7	141,809,687 (GRCm38)	intron	probably benign
R7091:Muc5ac	UTSW	7	141,809,687 (GRCm38)	intron	probably benign
R7092:Muc5ac	UTSW	7	141,809,687 (GRCm38)	intron	probably benign
R7092:Muc5ac	UTSW	7	141,809,648 (GRCm38)	intron	probably benign
R7110:Muc5ac	UTSW	7	141,799,822 (GRCm38)	missense	possibly damaging	0.95
R7117:Muc5ac	UTSW	7	141,813,822 (GRCm38)	nonsense	probably null
R7238:Muc5ac	UTSW	7	141,809,687 (GRCm38)	intron	probably benign
R7238:Muc5ac	UTSW	7	141,809,517 (GRCm38)	missense	unknown
R7396:Muc5ac	UTSW	7	141,808,415 (GRCm38)	missense	unknown
R7456:Muc5ac	UTSW	7	141,793,167 (GRCm38)	missense	probably benign	0.32
R7477:Muc5ac	UTSW	7	141,816,282 (GRCm38)	missense	possibly damaging	0.72
R7530:Muc5ac	UTSW	7	141,813,799 (GRCm38)	missense	possibly damaging	0.51
R7545:Muc5ac	UTSW	7	141,808,668 (GRCm38)	missense	unknown
R7604:Muc5ac	UTSW	7	141,809,709 (GRCm38)	missense	unknown
R7635:Muc5ac	UTSW	7	141,805,676 (GRCm38)	missense	probably damaging	0.98
R7635:Muc5ac	UTSW	7	141,805,753 (GRCm38)	missense	possibly damaging	0.53
R7650:Muc5ac	UTSW	7	141,809,422 (GRCm38)	missense	unknown
R7651:Muc5ac	UTSW	7	141,796,254 (GRCm38)	missense	possibly damaging	0.92
R7685:Muc5ac	UTSW	7	141,809,383 (GRCm38)	missense	unknown
R7720:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7749:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7750:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7751:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7754:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7798:Muc5ac	UTSW	7	141,794,041 (GRCm38)	critical splice donor site	probably null
R7835:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7837:Muc5ac	UTSW	7	141,815,963 (GRCm38)	missense	possibly damaging	0.53
R7858:Muc5ac	UTSW	7	141,803,429 (GRCm38)	missense	possibly damaging	0.51
R7866:Muc5ac	UTSW	7	141,795,852 (GRCm38)	missense	probably benign	0.00
R7874:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7876:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7877:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7881:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7884:Muc5ac	UTSW	7	141,809,303 (GRCm38)	missense	unknown
R7921:Muc5ac	UTSW	7	141,809,687 (GRCm38)	intron	probably benign
R7976:Muc5ac	UTSW	7	141,809,791 (GRCm38)	missense	unknown
R8104:Muc5ac	UTSW	7	141,804,783 (GRCm38)	missense	possibly damaging	0.96
R8177:Muc5ac	UTSW	7	141,807,331 (GRCm38)	missense	probably damaging	1.00
R8214:Muc5ac	UTSW	7	141,802,948 (GRCm38)	missense	possibly damaging	0.53
R8292:Muc5ac	UTSW	7	141,809,263 (GRCm38)	missense	unknown
R8386:Muc5ac	UTSW	7	141,807,634 (GRCm38)	missense	possibly damaging	0.93
R8400:Muc5ac	UTSW	7	141,810,476 (GRCm38)	missense	probably damaging	0.99
R8504:Muc5ac	UTSW	7	141,807,155 (GRCm38)	missense	probably damaging	1.00
R8709:Muc5ac	UTSW	7	141,816,926 (GRCm38)	missense	possibly damaging	0.96
R8725:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R8727:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
R8754:Muc5ac	UTSW	7	141,800,271 (GRCm38)	missense	possibly damaging	0.85
R8769:Muc5ac	UTSW	7	141,818,872 (GRCm38)	missense	probably damaging	1.00
R8933:Muc5ac	UTSW	7	141,789,756 (GRCm38)	missense	possibly damaging	0.59
R8939:Muc5ac	UTSW	7	141,793,354 (GRCm38)	missense	probably damaging	0.98
R9049:Muc5ac	UTSW	7	141,808,975 (GRCm38)	missense	unknown
R9124:Muc5ac	UTSW	7	141,809,792 (GRCm38)	missense	unknown
R9131:Muc5ac	UTSW	7	141,809,792 (GRCm38)	missense	unknown
R9132:Muc5ac	UTSW	7	141,809,792 (GRCm38)	missense	unknown
R9135:Muc5ac	UTSW	7	141,798,481 (GRCm38)	missense	probably damaging	0.99
R9156:Muc5ac	UTSW	7	141,809,792 (GRCm38)	missense	unknown
R9157:Muc5ac	UTSW	7	141,809,792 (GRCm38)	missense	unknown
R9159:Muc5ac	UTSW	7	141,809,792 (GRCm38)	missense	unknown
R9160:Muc5ac	UTSW	7	141,809,792 (GRCm38)	missense	unknown
R9161:Muc5ac	UTSW	7	141,799,289 (GRCm38)	missense	possibly damaging	0.53
R9175:Muc5ac	UTSW	7	141,812,356 (GRCm38)	missense	possibly damaging	0.92
R9183:Muc5ac	UTSW	7	141,798,900 (GRCm38)	missense	possibly damaging	0.71
R9218:Muc5ac	UTSW	7	141,807,361 (GRCm38)	missense	probably damaging	0.99
R9219:Muc5ac	UTSW	7	141,817,063 (GRCm38)	nonsense	probably null
R9239:Muc5ac	UTSW	7	141,800,217 (GRCm38)	missense	probably damaging	0.99
R9246:Muc5ac	UTSW	7	141,810,478 (GRCm38)	missense	probably benign	0.11
R9287:Muc5ac	UTSW	7	141,807,889 (GRCm38)	missense	probably damaging	0.99
R9320:Muc5ac	UTSW	7	141,815,518 (GRCm38)	missense	probably benign	0.01
R9327:Muc5ac	UTSW	7	141,811,692 (GRCm38)	missense	possibly damaging	0.86
R9428:Muc5ac	UTSW	7	141,808,822 (GRCm38)	missense	unknown
R9430:Muc5ac	UTSW	7	141,808,832 (GRCm38)	missense	unknown
R9454:Muc5ac	UTSW	7	141,808,694 (GRCm38)	missense	unknown
R9483:Muc5ac	UTSW	7	141,811,728 (GRCm38)	nonsense	probably null
R9581:Muc5ac	UTSW	7	141,810,062 (GRCm38)	missense	unknown
R9610:Muc5ac	UTSW	7	141,796,341 (GRCm38)	missense	possibly damaging	0.86
R9642:Muc5ac	UTSW	7	141,795,864 (GRCm38)	missense	possibly damaging	0.71
R9684:Muc5ac	UTSW	7	141,811,061 (GRCm38)	missense	probably benign	0.41
R9760:Muc5ac	UTSW	7	141,807,248 (GRCm38)	missense	probably benign	0.05
R9778:Muc5ac	UTSW	7	141,795,284 (GRCm38)	nonsense	probably null
X0060:Muc5ac	UTSW	7	141,803,333 (GRCm38)	missense	possibly damaging	0.71
Z1088:Muc5ac	UTSW	7	141,811,692 (GRCm38)	missense	possibly damaging	0.86
Z1088:Muc5ac	UTSW	7	141,809,744 (GRCm38)	intron	probably benign
Z1177:Muc5ac	UTSW	7	141,818,040 (GRCm38)	missense	probably benign	0.33
Z1177:Muc5ac	UTSW	7	141,809,224 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GGGAAGCCATATCTCGAAGAC -3'
(R):5'- ATTGTGAATGTCAGGGGCAATG -3'

Sequencing Primer
(F):5'- AAGACTTACACCTGGCCTGTG -3'
(R):5'- TGTCAGGGGCAATGAAGCATTAAG -3'

Posted On

2015-11-11