Mutagenetix > Incidental Mutation

Incidental Mutation 'R4729:Trim66'

358682

Institutional Source

Beutler Lab

Gene Symbol

Trim66

Ensembl Gene

ENSMUSG00000031026

Gene Name

tripartite motif-containing 66

Synonyms

Tif1d, D7H11orf29

MMRRC Submission

041603-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.226) question?

Stock #

R4729 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

109449006-109508134 bp(-) (GRCm38)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 109456060 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000102352 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033339] [ENSMUST00000106739] [ENSMUST00000106741]

AlphaFold

Q924W6

Predicted Effect

probably null
Transcript: ENSMUST00000033339

SMART Domains

Protein: ENSMUSP00000033339
Gene: ENSMUSG00000031026

Domain	Start	End	E-Value	Type
PHD	4	69	7.77e0	SMART
BBC	108	234	1.61e-39	SMART
low complexity region	318	333	N/A	INTRINSIC
low complexity region	452	486	N/A	INTRINSIC
low complexity region	517	530	N/A	INTRINSIC
low complexity region	568	581	N/A	INTRINSIC
PHD	998	1041	4.09e-10	SMART
BROMO	1069	1175	8.22e-27	SMART
low complexity region	1185	1199	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106739

SMART Domains

Protein: ENSMUSP00000102350
Gene: ENSMUSG00000031026

Domain	Start	End	E-Value	Type
PHD	4	69	7.77e0	SMART
BBC	108	234	1.61e-39	SMART
low complexity region	318	333	N/A	INTRINSIC
low complexity region	452	486	N/A	INTRINSIC
low complexity region	517	530	N/A	INTRINSIC
low complexity region	568	581	N/A	INTRINSIC
PHD	998	1041	4.09e-10	SMART
BROMO	1069	1175	8.22e-27	SMART
low complexity region	1185	1199	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106741

SMART Domains

Protein: ENSMUSP00000102352
Gene: ENSMUSG00000031026

Domain	Start	End	E-Value	Type
RING	28	78	2.38e-2	SMART
BBOX	102	140	1.48e0	SMART
PHD	106	171	7.77e0	SMART
RING	107	170	4.38e0	SMART
BBOX	162	203	4.21e-3	SMART
BBC	210	336	1.61e-39	SMART
low complexity region	420	435	N/A	INTRINSIC
low complexity region	554	588	N/A	INTRINSIC
low complexity region	619	632	N/A	INTRINSIC
low complexity region	670	683	N/A	INTRINSIC
PHD	1100	1143	4.09e-10	SMART
BROMO	1171	1277	8.22e-27	SMART
low complexity region	1287	1301	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930562C15Rik	T	A	16: 4,849,323 (GRCm38)	L220H	unknown	Het
Amt	T	A	9: 108,300,652 (GRCm38)	L272Q	probably damaging	Het
Ank2	G	C	3: 126,976,896 (GRCm38)	Y894*	probably null	Het
Ankzf1	T	A	1: 75,194,264 (GRCm38)	F105I	probably damaging	Het
Aox4	C	A	1: 58,259,077 (GRCm38)	Y1067*	probably null	Het
BC034090	T	A	1: 155,225,090 (GRCm38)	Q476L	probably damaging	Het
Bhmt	G	T	13: 93,627,363 (GRCm38)	R57S	probably damaging	Het
Cacna1c	A	T	6: 118,656,175 (GRCm38)	F964L	probably damaging	Het
Celsr3	A	C	9: 108,847,652 (GRCm38)	S396R	probably benign	Het
Cpne4	C	A	9: 104,922,556 (GRCm38)	Q191K	probably damaging	Het
Csnk1g2	T	C	10: 80,639,204 (GRCm38)	Y352H	probably benign	Het
D630003M21Rik	A	G	2: 158,216,703 (GRCm38)	S426P	probably damaging	Het
Dido1	G	T	2: 180,687,650 (GRCm38)	N326K	probably benign	Het
E2f2	T	A	4: 136,184,449 (GRCm38)	I257N	probably damaging	Het
Elfn1	T	G	5: 139,973,658 (GRCm38)	F806V	probably damaging	Het
Eml6	T	A	11: 29,833,204 (GRCm38)	Y559F	probably damaging	Het
Erich6	A	G	3: 58,636,059 (GRCm38)		probably null	Het
Fam45a	T	A	19: 60,834,871 (GRCm38)	F315I	probably benign	Het
Fam78a	T	C	2: 32,082,605 (GRCm38)	N101S	probably damaging	Het
Fam83h	A	G	15: 76,002,336 (GRCm38)	S1051P	probably benign	Het
Galns	C	T	8: 122,603,456 (GRCm38)	G112D	probably damaging	Het
Gm7075	A	T	10: 63,421,465 (GRCm38)	V92E	probably damaging	Het
Gm9923	A	G	10: 72,309,694 (GRCm38)	K125R	probably damaging	Het
Hectd3	G	A	4: 116,997,218 (GRCm38)	V326M	probably damaging	Het
Iars2	A	G	1: 185,316,051 (GRCm38)	S495P	possibly damaging	Het
Ice1	T	C	13: 70,606,384 (GRCm38)	R528G	probably damaging	Het
Igkv12-38	T	A	6: 69,943,384 (GRCm38)	Y50F	possibly damaging	Het
Inpp5j	T	C	11: 3,495,025 (GRCm38)	S883G	probably damaging	Het
Irf8	T	C	8: 120,753,439 (GRCm38)	S139P	probably damaging	Het
Itpr2	A	G	6: 146,373,173 (GRCm38)	F837S	probably damaging	Het
Kcnq4	G	T	4: 120,713,074 (GRCm38)	D357E	possibly damaging	Het
Klhl1	A	G	14: 96,280,148 (GRCm38)	L364P	probably damaging	Het
Krt7	A	G	15: 101,420,558 (GRCm38)	I309V	probably benign	Het
Krt76	G	T	15: 101,889,081 (GRCm38)	A281D	probably damaging	Het
Lancl2	T	C	6: 57,737,712 (GRCm38)	F430L	probably damaging	Het
Llgl2	T	C	11: 115,848,299 (GRCm38)	V332A	probably damaging	Het
Lrrc26	A	T	2: 25,290,064 (GRCm38)	T26S	probably benign	Het
Lrrk1	T	A	7: 66,262,293 (GRCm38)	M1840L	probably benign	Het
Lyst	G	A	13: 13,637,901 (GRCm38)	C966Y	probably damaging	Het
Mad1l1	T	A	5: 140,261,511 (GRCm38)	S354C	possibly damaging	Het
Mbtps1	C	T	8: 119,525,420 (GRCm38)	G577D	probably damaging	Het
Mycbp2	C	T	14: 103,188,591 (GRCm38)	R2366H	probably damaging	Het
Myo18a	T	G	11: 77,777,685 (GRCm38)		probably null	Het
Naip5	G	A	13: 100,222,131 (GRCm38)	R866C	possibly damaging	Het
Nav2	T	A	7: 49,452,819 (GRCm38)	V455E	probably benign	Het
Neb	A	G	2: 52,263,662 (GRCm38)	I2417T	possibly damaging	Het
Nme5	C	T	18: 34,569,837 (GRCm38)	A133T	probably benign	Het
Nradd	T	C	9: 110,621,911 (GRCm38)	D51G	possibly damaging	Het
Pard3b	A	G	1: 62,211,684 (GRCm38)	R591G	probably damaging	Het
Pcbp4	T	C	9: 106,460,730 (GRCm38)	F73S	probably damaging	Het
Pcdhb11	C	T	18: 37,422,366 (GRCm38)	Q250*	probably null	Het
Pde1c	T	A	6: 56,072,209 (GRCm38)	K766N	probably damaging	Het
Phf21b	G	A	15: 84,854,741 (GRCm38)	Q40*	probably null	Het
Prr14	A	G	7: 127,474,696 (GRCm38)	D244G	probably benign	Het
Rab11fip5	C	T	6: 85,374,267 (GRCm38)	A88T	probably damaging	Het
Rad54l2	A	T	9: 106,716,118 (GRCm38)	S428T	probably benign	Het
Rdh9	A	G	10: 127,776,752 (GRCm38)	I90V	probably benign	Het
Rhag	G	T	17: 40,828,401 (GRCm38)	G76C	probably damaging	Het
Riok3	T	A	18: 12,128,927 (GRCm38)	V6E	possibly damaging	Het
Scn10a	A	G	9: 119,671,526 (GRCm38)	I229T	probably damaging	Het
Slco1a1	G	T	6: 141,908,969 (GRCm38)	T652K	probably benign	Het
Slit1	T	A	19: 41,647,020 (GRCm38)	I345F	probably damaging	Het
Snrnp200	A	T	2: 127,232,937 (GRCm38)	I1477F	probably damaging	Het
Snx31	G	A	15: 36,523,552 (GRCm38)	T328I	possibly damaging	Het
Strn	A	G	17: 78,657,961 (GRCm38)	F634S	probably damaging	Het
Tmem101	C	T	11: 102,156,329 (GRCm38)	G6R	probably benign	Het
Tmem199	C	G	11: 78,508,680 (GRCm38)	G131R	probably benign	Het
Ttn	G	T	2: 76,851,585 (GRCm38)		probably benign	Het
Upk3bl	T	C	5: 136,057,393 (GRCm38)	L61P	probably benign	Het
Vmn2r108	A	G	17: 20,472,370 (GRCm38)	Y75H	probably damaging	Het
Vmn2r91	T	A	17: 18,107,644 (GRCm38)	I500K	probably damaging	Het
Vps18	C	T	2: 119,293,791 (GRCm38)	R400C	probably damaging	Het
Zeb1	C	T	18: 5,767,286 (GRCm38)	P599L	probably damaging	Het
Zfhx4	G	T	3: 5,399,497 (GRCm38)	A1572S	probably damaging	Het
Zfp677	T	A	17: 21,397,418 (GRCm38)	C246S	possibly damaging	Het
Zfp719	T	A	7: 43,590,410 (GRCm38)	I474N	probably damaging	Het

Other mutations in Trim66

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01539:Trim66	APN	7	109,455,066 (GRCm38)	missense	probably benign	0.02
IGL01758:Trim66	APN	7	109,486,045 (GRCm38)	critical splice donor site	probably null
IGL01982:Trim66	APN	7	109,458,763 (GRCm38)	missense	probably benign	0.00
IGL01983:Trim66	APN	7	109,458,251 (GRCm38)	nonsense	probably null
IGL02149:Trim66	APN	7	109,460,902 (GRCm38)	missense	possibly damaging	0.66
IGL02392:Trim66	APN	7	109,460,274 (GRCm38)	missense	probably benign	0.01
IGL02483:Trim66	APN	7	109,477,630 (GRCm38)	splice site	probably benign
IGL02832:Trim66	APN	7	109,460,497 (GRCm38)	missense	probably damaging	1.00
IGL02945:Trim66	APN	7	109,460,176 (GRCm38)	nonsense	probably null
IGL03085:Trim66	APN	7	109,458,745 (GRCm38)	missense	probably benign	0.17
PIT1430001:Trim66	UTSW	7	109,475,247 (GRCm38)	missense	probably damaging	0.99
R0326:Trim66	UTSW	7	109,460,172 (GRCm38)	missense	probably benign	0.00
R0358:Trim66	UTSW	7	109,460,176 (GRCm38)	nonsense	probably null
R0401:Trim66	UTSW	7	109,475,264 (GRCm38)	missense	probably damaging	0.98
R0470:Trim66	UTSW	7	109,457,542 (GRCm38)	splice site	probably benign
R0568:Trim66	UTSW	7	109,460,695 (GRCm38)	missense	probably benign	0.00
R0669:Trim66	UTSW	7	109,454,992 (GRCm38)	intron	probably benign
R0980:Trim66	UTSW	7	109,455,670 (GRCm38)	missense	probably damaging	1.00
R1015:Trim66	UTSW	7	109,455,233 (GRCm38)	missense	probably damaging	1.00
R1078:Trim66	UTSW	7	109,472,319 (GRCm38)	missense	probably damaging	1.00
R1099:Trim66	UTSW	7	109,475,454 (GRCm38)	missense	probably benign	0.34
R1181:Trim66	UTSW	7	109,484,577 (GRCm38)	critical splice donor site	probably null
R1497:Trim66	UTSW	7	109,484,619 (GRCm38)	missense	probably benign	0.00
R1583:Trim66	UTSW	7	109,455,080 (GRCm38)	missense	probably damaging	1.00
R1843:Trim66	UTSW	7	109,475,839 (GRCm38)	missense	probably damaging	0.99
R1998:Trim66	UTSW	7	109,484,577 (GRCm38)	critical splice donor site	probably null
R2016:Trim66	UTSW	7	109,472,232 (GRCm38)	critical splice donor site	probably null
R2143:Trim66	UTSW	7	109,475,113 (GRCm38)	missense	probably damaging	0.98
R2144:Trim66	UTSW	7	109,475,113 (GRCm38)	missense	probably damaging	0.98
R2145:Trim66	UTSW	7	109,475,113 (GRCm38)	missense	probably damaging	0.98
R3945:Trim66	UTSW	7	109,472,268 (GRCm38)	missense	possibly damaging	0.94
R4012:Trim66	UTSW	7	109,458,131 (GRCm38)	missense	probably damaging	0.98
R4464:Trim66	UTSW	7	109,477,690 (GRCm38)	missense	possibly damaging	0.51
R4473:Trim66	UTSW	7	109,481,995 (GRCm38)	missense	probably damaging	1.00
R4730:Trim66	UTSW	7	109,483,069 (GRCm38)	missense	probably damaging	1.00
R4775:Trim66	UTSW	7	109,457,589 (GRCm38)	nonsense	probably null
R4819:Trim66	UTSW	7	109,457,586 (GRCm38)	missense	probably damaging	1.00
R5269:Trim66	UTSW	7	109,457,590 (GRCm38)	missense	probably benign	0.00
R5557:Trim66	UTSW	7	109,483,737 (GRCm38)	missense	probably benign	0.06
R5832:Trim66	UTSW	7	109,455,202 (GRCm38)	missense	probably damaging	1.00
R6220:Trim66	UTSW	7	109,483,093 (GRCm38)	missense	probably damaging	0.97
R6243:Trim66	UTSW	7	109,460,274 (GRCm38)	missense	probably benign	0.01
R6374:Trim66	UTSW	7	109,486,062 (GRCm38)	missense	probably benign
R6450:Trim66	UTSW	7	109,460,738 (GRCm38)	missense	probably benign	0.09
R6543:Trim66	UTSW	7	109,475,879 (GRCm38)	missense	probably benign	0.01
R6788:Trim66	UTSW	7	109,477,754 (GRCm38)	missense	probably damaging	1.00
R6842:Trim66	UTSW	7	109,460,776 (GRCm38)	missense	probably benign	0.00
R7169:Trim66	UTSW	7	109,455,121 (GRCm38)	missense	probably benign	0.25
R7257:Trim66	UTSW	7	109,460,244 (GRCm38)	missense	probably damaging	1.00
R7328:Trim66	UTSW	7	109,457,751 (GRCm38)	missense	probably damaging	0.99
R7616:Trim66	UTSW	7	109,483,749 (GRCm38)	missense	probably damaging	0.99
R8423:Trim66	UTSW	7	109,475,392 (GRCm38)	missense	possibly damaging	0.77
R8855:Trim66	UTSW	7	109,481,981 (GRCm38)	missense	probably damaging	1.00
R9130:Trim66	UTSW	7	109,477,689 (GRCm38)	missense	possibly damaging	0.90
R9137:Trim66	UTSW	7	109,475,123 (GRCm38)	missense	probably damaging	0.99
R9640:Trim66	UTSW	7	109,475,618 (GRCm38)	missense	probably damaging	1.00
RF013:Trim66	UTSW	7	109,460,753 (GRCm38)	missense	probably damaging	0.99
RF024:Trim66	UTSW	7	109,460,740 (GRCm38)	missense	possibly damaging	0.62

Predicted Primers

PCR Primer
(F):5'- AATAATGCCTGGCCTTGGGG -3'
(R):5'- AAACTGCCTTCTTAGGGTGGC -3'

Sequencing Primer
(F):5'- CTGGCCTTGGGGAAGGAG -3'
(R):5'- CTGGCATCCTTGAGGTCAGAG -3'

Posted On

2015-11-11