Mutagenetix > Incidental Mutation

Incidental Mutation 'R4733:Triobp'

359009

Institutional Source

Beutler Lab

Gene Symbol

Triobp

Ensembl Gene

ENSMUSG00000033088

Gene Name

TRIO and F-actin binding protein

Synonyms

EST478828, Mus EST 478828, Tara

MMRRC Submission

042023-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4733 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

78947724-79005869 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 78967113 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Lysine at position 489 (R489K)

Ref Sequence

ENSEMBL: ENSMUSP00000155397 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109689] [ENSMUST00000109690] [ENSMUST00000140228]

AlphaFold

Q99KW3

Predicted Effect

probably damaging
Transcript: ENSMUST00000109689
AA Change: R489K

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000105311
Gene: ENSMUSG00000033088
AA Change: R489K

Domain	Start	End	E-Value	Type
low complexity region	130	154	N/A	INTRINSIC
low complexity region	291	311	N/A	INTRINSIC
internal_repeat_1	312	394	7.43e-13	PROSPERO
internal_repeat_1	390	540	7.43e-13	PROSPERO
low complexity region	585	600	N/A	INTRINSIC
low complexity region	638	657	N/A	INTRINSIC
low complexity region	697	729	N/A	INTRINSIC
low complexity region	767	777	N/A	INTRINSIC
low complexity region	885	901	N/A	INTRINSIC
low complexity region	903	923	N/A	INTRINSIC
low complexity region	995	1017	N/A	INTRINSIC
low complexity region	1054	1068	N/A	INTRINSIC
low complexity region	1221	1235	N/A	INTRINSIC
PH	1395	1492	6.2e-19	SMART
coiled coil region	1665	1692	N/A	INTRINSIC
coiled coil region	1727	1765	N/A	INTRINSIC
coiled coil region	1789	1851	N/A	INTRINSIC
coiled coil region	1885	1964	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000109690
AA Change: R489K

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000105312
Gene: ENSMUSG00000033088
AA Change: R489K

Domain	Start	End	E-Value	Type
low complexity region	130	154	N/A	INTRINSIC
low complexity region	291	311	N/A	INTRINSIC
internal_repeat_1	312	394	9.24e-13	PROSPERO
internal_repeat_1	390	540	9.24e-13	PROSPERO
low complexity region	585	600	N/A	INTRINSIC
low complexity region	638	657	N/A	INTRINSIC
low complexity region	697	729	N/A	INTRINSIC
low complexity region	767	777	N/A	INTRINSIC
low complexity region	885	901	N/A	INTRINSIC
low complexity region	903	923	N/A	INTRINSIC
low complexity region	995	1017	N/A	INTRINSIC
low complexity region	1054	1068	N/A	INTRINSIC
low complexity region	1221	1235	N/A	INTRINSIC
PH	1441	1538	6.2e-19	SMART
coiled coil region	1711	1738	N/A	INTRINSIC
coiled coil region	1773	1811	N/A	INTRINSIC
coiled coil region	1835	1897	N/A	INTRINSIC
coiled coil region	1931	2010	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000140228
AA Change: R489K

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.8%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a protein that interacts with trio, which is involved with neural tissue development and controlling actin cytoskeleton organization, cell motility, and cell growth. The encoded protein also associates with F-actin and stabilizes F-actin structures. Domains contained in this encoded protein are an N-terminal pleckstrin homology domain and a C-terminal coiled-coil region. Mutations in the human gene have been associated with a form of autosomal recessive nonsyndromic deafness. Multiple alternatively spliced transcript variants have been described [provided by RefSeq, Sep 2012]
PHENOTYPE: Mice homozygous for gene trapped alleles exhibit embryonic lethality. Mice homozygous for a targeted allele eliminating isoforms 4 and 5 exhibit profound deafness associated with stereocilia fragility and degeneration. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 180 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700128F08Rik	T	A	9: 8,222,173 (GRCm38)		noncoding transcript	Het
3110035E14Rik	T	C	1: 9,606,976 (GRCm38)	S24P	probably benign	Het
4933409G03Rik	A	G	2: 68,614,721 (GRCm38)		probably benign	Het
Acan	T	C	7: 79,098,609 (GRCm38)	S1043P	probably damaging	Het
Ackr2	A	G	9: 121,909,183 (GRCm38)	Y208C	probably damaging	Het
Adam1a	T	A	5: 121,519,434 (GRCm38)	T599S	probably benign	Het
Adamts12	A	T	15: 11,270,662 (GRCm38)	S668C	probably damaging	Het
Adcy8	A	G	15: 64,754,862 (GRCm38)	V709A	possibly damaging	Het
Adgrf2	A	C	17: 42,710,754 (GRCm38)	I393S	probably damaging	Het
Ahnak	G	T	19: 9,007,301 (GRCm38)	G1983V	probably damaging	Het
Aim2	T	A	1: 173,463,876 (GRCm38)	D282E	possibly damaging	Het
Ak8	A	G	2: 28,760,071 (GRCm38)	Y370C	probably damaging	Het
Akap9	A	G	5: 4,013,901 (GRCm38)	D1750G	probably damaging	Het
Als2cl	T	A	9: 110,889,136 (GRCm38)	V315E	probably damaging	Het
Ankrd28	A	C	14: 31,755,741 (GRCm38)	C115G	probably benign	Het
Ap3b2	C	T	7: 81,471,932 (GRCm38)	A519T	probably damaging	Het
Apool	C	T	X: 112,372,200 (GRCm38)	T166I	probably damaging	Het
Arhgef2	A	T	3: 88,631,940 (GRCm38)	K65*	probably null	Het
Arhgef38	G	T	3: 133,132,269 (GRCm38)	Y633*	probably null	Het
Asah2	T	C	19: 31,995,358 (GRCm38)	N659S	probably benign	Het
Atf7ip2	A	G	16: 10,241,886 (GRCm38)	D430G	possibly damaging	Het
Atp8a1	G	A	5: 67,813,120 (GRCm38)	S92L	probably benign	Het
Bag4	C	T	8: 25,769,488 (GRCm38)	A228T	probably benign	Het
BC024978	T	A	7: 27,201,043 (GRCm38)	M149K	probably damaging	Het
C130073F10Rik	C	A	4: 101,890,710 (GRCm38)	S89I	probably benign	Het
Cacna1g	T	C	11: 94,443,215 (GRCm38)	T867A	probably damaging	Het
Ccdc88a	T	G	11: 29,485,906 (GRCm38)	N1276K	probably benign	Het
Cdc42bpg	T	A	19: 6,311,191 (GRCm38)	V282E	probably damaging	Het
Cdipt	T	A	7: 126,978,358 (GRCm38)	L92H	probably damaging	Het
Celsr2	T	A	3: 108,398,952 (GRCm38)	D2012V	probably damaging	Het
Cenpt	A	G	8: 105,847,136 (GRCm38)	V254A	probably benign	Het
Cep104	T	A	4: 153,988,426 (GRCm38)	D380E	probably damaging	Het
Cers5	C	T	15: 99,741,637 (GRCm38)	R123Q	probably benign	Het
Ces2h	A	G	8: 105,014,604 (GRCm38)	E76G	probably damaging	Het
Cfap77	T	A	2: 28,984,388 (GRCm38)	E143D	probably benign	Het
Chmp7	C	A	14: 69,732,296 (GRCm38)	R65L	probably damaging	Het
Cldnd2	T	A	7: 43,442,189 (GRCm38)	C65S	possibly damaging	Het
Clec2g	C	A	6: 128,981,879 (GRCm38)	Y142*	probably null	Het
Coch	A	T	12: 51,605,019 (GRCm38)	E549V	probably benign	Het
Cog7	T	C	7: 121,964,244 (GRCm38)	D215G	probably benign	Het
Col4a2	A	G	8: 11,446,197 (GRCm38)	H1606R	probably benign	Het
Col4a2	T	C	8: 11,414,779 (GRCm38)	V348A	probably benign	Het
Cpd	T	C	11: 76,811,794 (GRCm38)	N583D	probably damaging	Het
Cyp2d11	T	A	15: 82,389,227 (GRCm38)	Y481F	probably benign	Het
D130043K22Rik	T	C	13: 24,899,665 (GRCm38)	S1038P	probably damaging	Het
Deptor	C	A	15: 55,181,010 (GRCm38)	H191N	probably benign	Het
Dgkz	A	T	2: 91,938,339 (GRCm38)	I699N	probably damaging	Het
Dnah12	C	T	14: 26,781,784 (GRCm38)	T1653I	probably damaging	Het
Dnah7c	T	A	1: 46,770,173 (GRCm38)	N3550K	probably damaging	Het
Dnah8	A	G	17: 30,775,061 (GRCm38)	K3384R	probably null	Het
Dnhd1	C	A	7: 105,673,849 (GRCm38)	N521K	probably benign	Het
Drg2	T	C	11: 60,461,396 (GRCm38)		probably null	Het
Dync1h1	C	T	12: 110,649,507 (GRCm38)	Q3030*	probably null	Het
Efhb	G	A	17: 53,426,244 (GRCm38)	T533I	probably damaging	Het
Eif2d	T	C	1: 131,164,727 (GRCm38)	V374A	probably damaging	Het
Etfb	T	C	7: 43,444,200 (GRCm38)	V17A	probably damaging	Het
F5	C	T	1: 164,181,657 (GRCm38)	T332M	probably damaging	Het
Fcho1	T	C	8: 71,716,795 (GRCm38)	T156A	probably benign	Het
Fn1	A	T	1: 71,602,512 (GRCm38)		probably null	Het
Fnip2	A	T	3: 79,481,652 (GRCm38)	S561T	probably damaging	Het
Frs2	T	A	10: 117,074,093 (GRCm38)	T455S	probably benign	Het
Fry	G	A	5: 150,386,007 (GRCm38)	E639K		Het
Fto	T	A	8: 91,409,714 (GRCm38)	D205E	probably damaging	Het
Galntl6	G	A	8: 58,427,813 (GRCm38)	P147L	probably damaging	Het
Gigyf1	T	A	5: 137,524,770 (GRCm38)	D844E	probably benign	Het
Gle1	T	C	2: 29,940,232 (GRCm38)	S267P	probably damaging	Het
Glg1	G	A	8: 111,187,755 (GRCm38)	R466W	probably damaging	Het
Gm10277	T	C	11: 77,786,097 (GRCm38)		probably benign	Het
Gm5724	A	T	6: 141,723,179 (GRCm38)	M509K	probably benign	Het
Gm6871	T	C	7: 41,546,749 (GRCm38)	I39V	probably benign	Het
Gpr35	T	G	1: 92,983,385 (GRCm38)	I57S	probably damaging	Het
Gprin1	C	T	13: 54,739,957 (GRCm38)	G168E	possibly damaging	Het
Gtf3c2	G	T	5: 31,160,057 (GRCm38)	P586T	probably damaging	Het
Gucy1a2	A	T	9: 3,759,424 (GRCm38)	H410L	probably benign	Het
Gucy2c	A	G	6: 136,767,152 (GRCm38)	S150P	probably damaging	Het
Ifi214	T	C	1: 173,526,591 (GRCm38)	Q171R	probably benign	Het
Igkv4-50	T	C	6: 69,701,000 (GRCm38)	K40R	probably benign	Het
Igkv8-18	T	A	6: 70,356,296 (GRCm38)	I74N	probably damaging	Het
Il2ra	T	A	2: 11,676,920 (GRCm38)	M112K	probably benign	Het
Itpr2	A	G	6: 146,373,173 (GRCm38)	F837S	probably damaging	Het
Kbtbd7	T	C	14: 79,428,800 (GRCm38)	*691Q	probably null	Het
Kcnn2	T	A	18: 45,560,349 (GRCm38)	S331T	possibly damaging	Het
Khnyn	T	A	14: 55,886,489 (GRCm38)		probably null	Het
Kif26a	G	A	12: 112,175,573 (GRCm38)	A754T	probably benign	Het
Klra3	T	A	6: 130,327,132 (GRCm38)	Y199F	possibly damaging	Het
Lhx2	A	G	2: 38,359,991 (GRCm38)	K274R	probably damaging	Het
Lrp2	T	C	2: 69,533,555 (GRCm38)	I313V	probably benign	Het
Lrrfip1	A	T	1: 91,115,647 (GRCm38)	E591D	probably benign	Het
Lrrk2	A	C	15: 91,688,849 (GRCm38)	E200A	probably damaging	Het
Lrrk2	G	A	15: 91,765,747 (GRCm38)	E1696K	probably damaging	Het
Mast4	A	T	13: 102,772,572 (GRCm38)	M465K	probably damaging	Het
Moxd2	T	G	6: 40,878,859 (GRCm38)	I599L	probably benign	Het
Mug2	T	C	6: 122,071,872 (GRCm38)	S866P	probably damaging	Het
Ncam2	A	G	16: 81,434,884 (GRCm38)	T79A	possibly damaging	Het
Ncoa6	C	A	2: 155,421,301 (GRCm38)	Q404H	probably damaging	Het
Neb	A	G	2: 52,279,079 (GRCm38)	Y1815H	probably damaging	Het
Nell1	A	G	7: 50,856,217 (GRCm38)	D724G	probably damaging	Het
Nkx3-2	A	G	5: 41,762,144 (GRCm38)	V167A	probably benign	Het
Nsun3	C	A	16: 62,735,119 (GRCm38)	C348F	possibly damaging	Het
Obox6	A	T	7: 15,834,772 (GRCm38)	S60T	possibly damaging	Het
Olfr1	T	C	11: 73,395,695 (GRCm38)	D109G	probably benign	Het
Olfr1378	G	A	11: 50,969,266 (GRCm38)	V83M	possibly damaging	Het
Olfr138	T	A	17: 38,275,547 (GRCm38)	Y259N	probably damaging	Het
Olfr466	T	C	13: 65,152,653 (GRCm38)	V143A	possibly damaging	Het
Olfr744	T	A	14: 50,618,569 (GRCm38)	C116S	probably benign	Het
Olfr94	T	C	17: 37,197,024 (GRCm38)	T315A	probably damaging	Het
Olfr980	A	T	9: 40,006,268 (GRCm38)	I227N	probably damaging	Het
Pabpc1	A	T	15: 36,599,284 (GRCm38)	V389E	probably benign	Het
Pank4	A	T	4: 154,971,390 (GRCm38)	M291L	probably benign	Het
Pcf11	T	C	7: 92,658,833 (GRCm38)	D709G	probably benign	Het
Pcgf1	T	A	6: 83,079,957 (GRCm38)		probably benign	Het
Pcnx	A	G	12: 81,995,751 (GRCm38)	I2256V	probably benign	Het
Pex6	A	G	17: 46,722,288 (GRCm38)	D579G	probably benign	Het
Pex6	A	G	17: 46,724,707 (GRCm38)		probably null	Het
Piezo2	C	T	18: 63,030,401 (GRCm38)	A2149T	probably damaging	Het
Pik3c2g	A	G	6: 139,935,985 (GRCm38)	E781G	probably benign	Het
Pik3r4	G	A	9: 105,678,176 (GRCm38)	V1111I	possibly damaging	Het
Pkd1l2	T	A	8: 116,995,842 (GRCm38)		probably null	Het
Plekhg1	T	A	10: 3,957,506 (GRCm38)	S808T	probably benign	Het
Polr3c	A	T	3: 96,723,661 (GRCm38)	F148I	probably damaging	Het
Ppard	A	T	17: 28,286,443 (GRCm38)	T35S	probably benign	Het
Ptov1	T	C	7: 44,867,109 (GRCm38)	D134G	probably benign	Het
Ptprz1	T	A	6: 23,002,610 (GRCm38)	S1566R	probably benign	Het
Pum1	T	C	4: 130,718,193 (GRCm38)	S158P	probably benign	Het
Qk	T	C	17: 10,216,288 (GRCm38)	H269R	probably damaging	Het
Qrsl1	T	C	10: 43,876,663 (GRCm38)	Y388C	probably damaging	Het
Rapgef1	T	G	2: 29,689,160 (GRCm38)	I182S	probably damaging	Het
Ret	T	C	6: 118,163,193 (GRCm38)	S1013G	possibly damaging	Het
Rimbp3	A	G	16: 17,210,601 (GRCm38)	R630G	possibly damaging	Het
Ryr2	A	T	13: 11,577,909 (GRCm38)	M4653K	possibly damaging	Het
Sacm1l	G	A	9: 123,590,830 (GRCm38)	V553I	probably benign	Het
Sec31b	A	T	19: 44,532,677 (GRCm38)	S110T	probably damaging	Het
Serpina3k	G	A	12: 104,340,860 (GRCm38)	G117D	probably damaging	Het
Sesn2	T	C	4: 132,494,591 (GRCm38)	Y410C	probably damaging	Het
Slc24a1	G	A	9: 64,949,554 (GRCm38)	R24C	probably benign	Het
Slc35g2	A	C	9: 100,552,502 (GRCm38)	V372G	probably benign	Het
Slc7a7	T	C	14: 54,408,733 (GRCm38)	Y91C	probably damaging	Het
Slc7a9	T	A	7: 35,453,563 (GRCm38)	Y135*	probably null	Het
Slco4a1	A	G	2: 180,473,615 (GRCm38)	N662D	probably damaging	Het
Slfn4	T	A	11: 83,189,282 (GRCm38)		probably benign	Het
Slmap	T	C	14: 26,468,535 (GRCm38)	N156S	probably damaging	Het
Snx18	A	G	13: 113,617,774 (GRCm38)	S208P	probably benign	Het
Sorbs1	T	A	19: 40,314,689 (GRCm38)	R485S	probably benign	Het
Spib	A	G	7: 44,528,885 (GRCm38)	S154P	probably damaging	Het
Spty2d1	T	C	7: 46,996,110 (GRCm38)	D595G	probably damaging	Het
St7	T	A	6: 17,906,516 (GRCm38)		probably null	Het
Susd1	T	C	4: 59,428,029 (GRCm38)	T52A	possibly damaging	Het
Svs2	T	A	2: 164,237,123 (GRCm38)	D288V	possibly damaging	Het
Syt7	T	A	19: 10,442,924 (GRCm38)	I355N	probably damaging	Het
Tarm1	G	A	7: 3,496,900 (GRCm38)	Q145*	probably null	Het
Teddm2	T	A	1: 153,850,741 (GRCm38)	E76V	probably damaging	Het
Thsd7b	T	C	1: 129,613,186 (GRCm38)	S343P	probably damaging	Het
Tigd2	T	A	6: 59,211,415 (GRCm38)	H422Q	probably benign	Het
Tle1	T	C	4: 72,125,019 (GRCm38)	N538D	possibly damaging	Het
Tmc2	A	G	2: 130,261,397 (GRCm38)		probably null	Het
Tmtc1	A	C	6: 148,284,980 (GRCm38)		probably null	Het
Tns3	C	T	11: 8,450,986 (GRCm38)	R1104H	probably benign	Het
Trim6	T	C	7: 104,232,648 (GRCm38)	Y369H	probably damaging	Het
Trpv4	T	C	5: 114,622,753 (GRCm38)	D732G	possibly damaging	Het
Trrap	G	A	5: 144,816,570 (GRCm38)	V1883I	probably damaging	Het
Tsc2	C	A	17: 24,603,275 (GRCm38)	V1141F	possibly damaging	Het
Ttn	A	G	2: 76,899,827 (GRCm38)		probably benign	Het
Ttn	A	T	2: 76,943,011 (GRCm38)	M2395K	unknown	Het
Tyrp1	A	T	4: 80,844,935 (GRCm38)	D353V	possibly damaging	Het
Ubd	A	C	17: 37,195,702 (GRCm38)	T160P	probably benign	Het
Ugt2b36	G	T	5: 87,081,538 (GRCm38)	Y156*	probably null	Het
Ulk4	G	A	9: 121,263,638 (GRCm38)	R178*	probably null	Het
Unc13d	A	G	11: 116,073,582 (GRCm38)	V312A	possibly damaging	Het
Urb2	G	T	8: 124,028,897 (GRCm38)	A448S	probably damaging	Het
Urod	G	A	4: 116,991,673 (GRCm38)	A92V	possibly damaging	Het
Vmn1r33	T	A	6: 66,611,819 (GRCm38)	R250S	probably benign	Het
Vmn1r87	A	T	7: 13,132,327 (GRCm38)	M11K	possibly damaging	Het
Vmn2r77	C	T	7: 86,800,987 (GRCm38)	T147I	probably benign	Het
Vstm4	A	G	14: 32,917,902 (GRCm38)	K96E	possibly damaging	Het
Washc4	A	T	10: 83,574,479 (GRCm38)	M644L	probably benign	Het
Wwp1	T	C	4: 19,661,990 (GRCm38)	D172G	probably benign	Het
Zbtb38	A	T	9: 96,687,684 (GRCm38)	V449E	probably damaging	Het
Zfp229	C	T	17: 21,745,286 (GRCm38)	H166Y	possibly damaging	Het
Zfp512b	A	G	2: 181,588,739 (GRCm38)	S453P	probably benign	Het
Zp2	C	A	7: 120,138,120 (GRCm38)	V282L	probably damaging	Het

Other mutations in Triobp

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01634:Triobp	APN	15	78,993,368 (GRCm38)	missense	probably damaging	1.00
IGL01904:Triobp	APN	15	78,967,364 (GRCm38)	missense	possibly damaging	0.80
IGL01957:Triobp	APN	15	78,972,647 (GRCm38)	critical splice donor site	probably null
IGL02085:Triobp	APN	15	78,974,297 (GRCm38)	splice site	probably benign
IGL02260:Triobp	APN	15	78,966,362 (GRCm38)	missense	probably benign	0.00
IGL02498:Triobp	APN	15	78,961,043 (GRCm38)	missense	probably benign	0.01
IGL02551:Triobp	APN	15	78,973,489 (GRCm38)	missense	probably benign
IGL02740:Triobp	APN	15	78,966,689 (GRCm38)	missense	probably benign	0.21
IGL02810:Triobp	APN	15	79,002,203 (GRCm38)	missense	possibly damaging	0.95
IGL03063:Triobp	APN	15	78,990,884 (GRCm38)	missense	probably damaging	1.00
FR4304:Triobp	UTSW	15	78,993,387 (GRCm38)	unclassified	probably benign
FR4340:Triobp	UTSW	15	78,993,390 (GRCm38)	unclassified	probably benign
FR4342:Triobp	UTSW	15	78,993,392 (GRCm38)	unclassified	probably benign
FR4449:Triobp	UTSW	15	78,993,389 (GRCm38)	unclassified	probably benign
FR4548:Triobp	UTSW	15	78,993,390 (GRCm38)	unclassified	probably benign
FR4548:Triobp	UTSW	15	78,993,387 (GRCm38)	unclassified	probably benign
R0276:Triobp	UTSW	15	78,973,676 (GRCm38)	missense	probably benign	0.09
R0309:Triobp	UTSW	15	78,976,540 (GRCm38)	missense	probably damaging	1.00
R0433:Triobp	UTSW	15	78,968,201 (GRCm38)	missense	possibly damaging	0.69
R0464:Triobp	UTSW	15	78,966,986 (GRCm38)	missense	possibly damaging	0.71
R0525:Triobp	UTSW	15	78,973,898 (GRCm38)	missense	possibly damaging	0.93
R0665:Triobp	UTSW	15	78,973,898 (GRCm38)	missense	possibly damaging	0.93
R0689:Triobp	UTSW	15	78,959,988 (GRCm38)	nonsense	probably null
R1149:Triobp	UTSW	15	78,966,479 (GRCm38)	missense	probably benign	0.00
R1149:Triobp	UTSW	15	78,966,479 (GRCm38)	missense	probably benign	0.00
R1151:Triobp	UTSW	15	78,966,479 (GRCm38)	missense	probably benign	0.00
R1152:Triobp	UTSW	15	78,966,479 (GRCm38)	missense	probably benign	0.00
R1510:Triobp	UTSW	15	79,003,767 (GRCm38)	missense	probably damaging	1.00
R1519:Triobp	UTSW	15	78,973,738 (GRCm38)	missense	probably benign	0.00
R1642:Triobp	UTSW	15	79,002,148 (GRCm38)	missense	probably damaging	1.00
R1732:Triobp	UTSW	15	78,967,228 (GRCm38)	missense	possibly damaging	0.69
R1755:Triobp	UTSW	15	78,966,479 (GRCm38)	missense	probably benign	0.00
R1975:Triobp	UTSW	15	78,966,708 (GRCm38)	missense	probably benign
R2051:Triobp	UTSW	15	79,004,540 (GRCm38)	missense	probably damaging	1.00
R2073:Triobp	UTSW	15	78,973,895 (GRCm38)	missense	probably damaging	0.99
R2260:Triobp	UTSW	15	78,991,440 (GRCm38)	critical splice donor site	probably null
R2351:Triobp	UTSW	15	79,004,580 (GRCm38)	missense	probably benign	0.09
R2902:Triobp	UTSW	15	78,973,418 (GRCm38)	missense	possibly damaging	0.90
R3801:Triobp	UTSW	15	78,973,700 (GRCm38)	missense	probably benign	0.04
R3959:Triobp	UTSW	15	79,002,389 (GRCm38)	nonsense	probably null
R4003:Triobp	UTSW	15	78,959,977 (GRCm38)	unclassified	probably benign
R4084:Triobp	UTSW	15	78,973,671 (GRCm38)	missense	probably benign	0.19
R4482:Triobp	UTSW	15	78,966,563 (GRCm38)	missense	possibly damaging	0.87
R4592:Triobp	UTSW	15	78,967,095 (GRCm38)	missense	probably benign
R4662:Triobp	UTSW	15	78,993,269 (GRCm38)	missense	probably damaging	1.00
R4732:Triobp	UTSW	15	78,967,113 (GRCm38)	missense	probably damaging	0.99
R4789:Triobp	UTSW	15	78,991,028 (GRCm38)	missense	probably damaging	1.00
R4968:Triobp	UTSW	15	78,966,616 (GRCm38)	missense	probably benign	0.03
R4990:Triobp	UTSW	15	78,967,005 (GRCm38)	missense	probably benign	0.00
R5129:Triobp	UTSW	15	78,961,096 (GRCm38)	missense	probably benign	0.15
R5181:Triobp	UTSW	15	78,967,754 (GRCm38)	missense	probably benign	0.00
R5279:Triobp	UTSW	15	78,994,391 (GRCm38)	missense	possibly damaging	0.66
R5584:Triobp	UTSW	15	78,968,132 (GRCm38)	missense	possibly damaging	0.89
R5601:Triobp	UTSW	15	78,973,633 (GRCm38)	missense	probably damaging	1.00
R5810:Triobp	UTSW	15	78,968,267 (GRCm38)	missense	probably benign	0.07
R5969:Triobp	UTSW	15	78,967,540 (GRCm38)	missense	probably benign	0.05
R6722:Triobp	UTSW	15	79,001,565 (GRCm38)	missense	probably damaging	1.00
R6739:Triobp	UTSW	15	78,966,366 (GRCm38)	missense	possibly damaging	0.77
R6810:Triobp	UTSW	15	78,966,615 (GRCm38)	missense	possibly damaging	0.47
R7011:Triobp	UTSW	15	78,978,723 (GRCm38)	missense	probably damaging	0.98
R7015:Triobp	UTSW	15	78,994,060 (GRCm38)	missense	probably damaging	0.99
R7200:Triobp	UTSW	15	78,966,842 (GRCm38)	small deletion	probably benign
R7294:Triobp	UTSW	15	78,973,976 (GRCm38)	missense	probably damaging	0.99
R7688:Triobp	UTSW	15	78,961,111 (GRCm38)	splice site	probably null
R7805:Triobp	UTSW	15	78,974,004 (GRCm38)	missense	probably benign	0.37
R7972:Triobp	UTSW	15	78,967,986 (GRCm38)	missense	probably damaging	1.00
R7977:Triobp	UTSW	15	79,001,544 (GRCm38)	missense	probably damaging	1.00
R7987:Triobp	UTSW	15	79,001,544 (GRCm38)	missense	probably damaging	1.00
R7999:Triobp	UTSW	15	78,959,944 (GRCm38)	missense	probably damaging	0.99
R8344:Triobp	UTSW	15	78,958,275 (GRCm38)	missense	possibly damaging	0.67
R8348:Triobp	UTSW	15	78,994,126 (GRCm38)	missense	possibly damaging	0.85
R8446:Triobp	UTSW	15	78,994,126 (GRCm38)	missense	possibly damaging	0.85
R8448:Triobp	UTSW	15	78,994,126 (GRCm38)	missense	possibly damaging	0.85
R8469:Triobp	UTSW	15	78,967,019 (GRCm38)	missense	probably benign	0.00
R8491:Triobp	UTSW	15	78,994,126 (GRCm38)	missense	possibly damaging	0.85
R8492:Triobp	UTSW	15	78,994,126 (GRCm38)	missense	possibly damaging	0.85
R8493:Triobp	UTSW	15	78,994,126 (GRCm38)	missense	possibly damaging	0.85
R9424:Triobp	UTSW	15	78,960,066 (GRCm38)	missense	probably damaging	1.00
R9495:Triobp	UTSW	15	78,993,178 (GRCm38)	missense	probably damaging	1.00
R9514:Triobp	UTSW	15	78,993,178 (GRCm38)	missense	probably damaging	1.00
R9530:Triobp	UTSW	15	79,002,121 (GRCm38)	missense	probably damaging	1.00
R9550:Triobp	UTSW	15	78,973,877 (GRCm38)	missense	probably damaging	1.00
R9576:Triobp	UTSW	15	78,960,066 (GRCm38)	missense	probably damaging	1.00
R9646:Triobp	UTSW	15	79,003,734 (GRCm38)	missense	probably damaging	1.00
RF001:Triobp	UTSW	15	78,967,027 (GRCm38)	small insertion	probably benign
RF005:Triobp	UTSW	15	78,967,061 (GRCm38)	small insertion	probably benign
RF007:Triobp	UTSW	15	78,967,044 (GRCm38)	small insertion	probably benign
RF022:Triobp	UTSW	15	78,974,282 (GRCm38)	missense	probably benign	0.05
RF028:Triobp	UTSW	15	78,967,039 (GRCm38)	small insertion	probably benign
RF032:Triobp	UTSW	15	78,967,036 (GRCm38)	small insertion	probably benign
RF035:Triobp	UTSW	15	78,967,039 (GRCm38)	small insertion	probably benign
RF039:Triobp	UTSW	15	78,967,039 (GRCm38)	small insertion	probably benign
RF039:Triobp	UTSW	15	78,967,036 (GRCm38)	small insertion	probably benign
RF040:Triobp	UTSW	15	78,967,063 (GRCm38)	small insertion	probably benign
RF049:Triobp	UTSW	15	78,967,061 (GRCm38)	small insertion	probably benign
RF051:Triobp	UTSW	15	78,967,034 (GRCm38)	small insertion	probably benign
RF058:Triobp	UTSW	15	78,967,044 (GRCm38)	small insertion	probably benign
X0026:Triobp	UTSW	15	78,960,023 (GRCm38)	missense	possibly damaging	0.94
Z1177:Triobp	UTSW	15	79,002,181 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AACCCTAGGACTCCCTGTAC -3'
(R):5'- GGTTCCTGGAATTACTCCCCTG -3'

Sequencing Primer
(F):5'- TAGGACTCCCTGTACCCAGAG -3'
(R):5'- ATTGTCCTTTTGGGTGCAGCC -3'

Posted On

2015-11-11