|Institutional Source||Beutler Lab|
|Gene Name||cleavage stimulation factor, 3' pre-RNA, subunit 3|
|Is this an essential gene?||Probably essential (E-score: 0.965)|
|Stock #||R0332 (G1)|
|Chromosomal Location||104590523-104665429 bp(+) (GRCm38)|
|Type of Mutation||critical splice donor site (1 bp from exon)|
|DNA Base Change (assembly)||G to T at 104646467 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000028599 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000028599]|
|Predicted Effect||probably null
|Meta Mutation Damage Score||0.9594|
|Coding Region Coverage||
|Validation Efficiency||100% (63/63)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cstf3||
(F):5'- GCCTGTCTTCAGTGACTCTAACTTGG -3'
(R):5'- ACACAACCTCGTTGATAAACTCTTCGG -3'
(F):5'- ccaggctgcccttgaac -3'
(R):5'- GTTGATAAACTCTTCGGACAGCAG -3'