Mutagenetix > Incidental Mutation

Incidental Mutation 'R0245:Olfr177'

36197

Institutional Source

Beutler Lab

Gene Symbol

Olfr177

Ensembl Gene

ENSMUSG00000063137

Gene Name

olfactory receptor 177

Synonyms

GA_x54KRFPKG5P-55091371-55090442, MOR184-7

MMRRC Submission

038483-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.115) question?

Stock #

R0245 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

58870955-58874768 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 58872866 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Asparagine at position 95 (Y95N)

Ref Sequence

ENSEMBL: ENSMUSP00000150269 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072853] [ENSMUST00000217377]

AlphaFold

E9Q7W1

Predicted Effect

probably benign
Transcript: ENSMUST00000072853
AA Change: Y95N

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000072631
Gene: ENSMUSG00000063137
AA Change: Y95N

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.7e-53	PFAM
Pfam:7TM_GPCR_Srsx	35	254	8.3e-6	PFAM
Pfam:7tm_1	41	290	1.2e-17	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000205920
AA Change: Y95N

Predicted Effect

probably benign
Transcript: ENSMUST00000217377
AA Change: Y95N

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

Meta Mutation Damage Score

0.0754

Coding Region Coverage

1x: 99.4%
3x: 98.6%
10x: 96.7%
20x: 93.9%

Validation Efficiency

99% (73/74)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022B05Rik	A	G	8: 124,651,429		probably benign	Het
Adgrg6	T	A	10: 14,458,066		probably benign	Het
Adra2a	G	C	19: 54,047,409	V399L	probably damaging	Het
Arpc1b	A	G	5: 145,126,860	D306G	probably damaging	Het
Asic3	C	A	5: 24,413,838	R43S	probably damaging	Het
Astn2	T	C	4: 65,794,558	D615G	probably damaging	Het
BC024978	T	A	7: 27,200,603	C98S	possibly damaging	Het
Btbd2	A	T	10: 80,647,806	Y178N	probably damaging	Het
Cacna1c	T	C	6: 118,604,454	N1647D	probably benign	Het
Cacna2d4	A	T	6: 119,308,721	D803V	probably damaging	Het
Ccdc129	A	G	6: 55,898,007	E314G	probably damaging	Het
Cdh26	C	T	2: 178,481,632	R675C	possibly damaging	Het
Cdx2	C	A	5: 147,306,473	K170N	possibly damaging	Het
Cmpk2	A	T	12: 26,469,518	D56V	probably benign	Het
Dnah7a	T	C	1: 53,501,526	Y2563C	probably damaging	Het
Dock7	T	C	4: 99,055,349	D552G	possibly damaging	Het
E2f7	C	A	10: 110,774,795	S427*	probably null	Het
Eps8	T	C	6: 137,479,128	D785G	probably benign	Het
Ereg	G	A	5: 91,074,800	C14Y	possibly damaging	Het
Fah	A	C	7: 84,595,498	H222Q	probably benign	Het
Fbxw16	T	A	9: 109,436,168	S432C	possibly damaging	Het
Fdps	A	G	3: 89,093,771	S334P	possibly damaging	Het
Fgf7	A	G	2: 126,035,955	K81E	probably benign	Het
Gfra1	T	C	19: 58,300,554	N153S	possibly damaging	Het
Gm12355	T	A	11: 98,625,241		probably benign	Het
Golga1	A	G	2: 39,035,259	V351A	probably benign	Het
Got1	A	T	19: 43,504,507		probably benign	Het
Greb1	T	C	12: 16,696,456	Y1271C	probably damaging	Het
Gtf3c4	A	G	2: 28,834,964	I252T	possibly damaging	Het
Gucy1a1	A	G	3: 82,108,787	I298T	possibly damaging	Het
Hivep1	A	G	13: 42,164,290	I2081V	possibly damaging	Het
Hps3	A	T	3: 20,012,796	C535*	probably null	Het
Hspg2	T	C	4: 137,514,722	F589S	probably damaging	Het
Itgb8	T	A	12: 119,190,555	N249I	probably damaging	Het
Kdm4a	T	C	4: 118,175,689	D60G	probably benign	Het
Kng2	A	T	16: 23,012,181		probably benign	Het
March4	A	T	1: 72,534,781	D119E	probably benign	Het
Mrpl34	T	C	8: 71,465,075		probably benign	Het
Ncoa6	C	T	2: 155,391,211	G2059D	probably benign	Het
Nhsl1	A	G	10: 18,525,108	K660R	probably damaging	Het
Nr2c2ap	T	C	8: 70,131,578	V6A	possibly damaging	Het
Olfr1209	C	A	2: 88,909,875	D173Y	possibly damaging	Het
Olfr1302	A	G	2: 111,780,335	N5S	probably damaging	Het
Olfr1404	T	A	1: 173,215,957	I102N	possibly damaging	Het
Olfr853	C	A	9: 19,537,112	V273L	probably benign	Het
Oscar	C	T	7: 3,611,574		probably benign	Het
Pkhd1	T	C	1: 20,540,400	S1046G	probably benign	Het
Ptk6	T	C	2: 181,202,491	D5G	probably benign	Het
Rgs12	A	G	5: 35,030,080	H486R	probably benign	Het
Rnf111	C	T	9: 70,453,831		probably benign	Het
Rnf17	A	G	14: 56,438,609	Y309C	probably damaging	Het
Rnf19a	A	T	15: 36,253,032	I387N	probably damaging	Het
Safb	C	T	17: 56,606,025	R914C	probably damaging	Het
Sdk1	A	G	5: 141,954,958	T494A	probably benign	Het
Serac1	G	T	17: 6,051,756	D384E	probably damaging	Het
Sez6l	T	C	5: 112,475,566	M40V	probably benign	Het
Slc17a5	A	G	9: 78,540,924	I416T	probably benign	Het
Snapc1	C	T	12: 73,975,032	R81C	probably damaging	Het
Spata32	C	T	11: 103,209,095	A195T	probably damaging	Het
Srrd	A	G	5: 112,337,528		probably benign	Het
Supt3	T	C	17: 45,036,775	V208A	probably benign	Het
Taok3	G	A	5: 117,252,679		probably benign	Het
Tbxas1	G	T	6: 39,027,768	R316S	probably benign	Het
Tgfbrap1	A	T	1: 43,075,592	I116N	possibly damaging	Het
Tm7sf3	T	C	6: 146,618,609	T260A	possibly damaging	Het
Top2a	T	C	11: 99,010,096	I556V	probably benign	Het
Uroc1	A	G	6: 90,344,197	M252V	probably damaging	Het
Xpo4	G	T	14: 57,630,240	H183Q	probably damaging	Het
Zcchc17	T	A	4: 130,337,154	I81L	probably benign	Het
Zfp455	A	T	13: 67,207,835	Y389F	probably damaging	Het

Other mutations in Olfr177

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02084:Olfr177	APN	16	58873036	missense	probably damaging	1.00
IGL02223:Olfr177	APN	16	58872694	missense	probably damaging	1.00
R0131:Olfr177	UTSW	16	58872906	missense	probably benign	0.01
R0131:Olfr177	UTSW	16	58872906	missense	probably benign	0.01
R0132:Olfr177	UTSW	16	58872906	missense	probably benign	0.01
R0717:Olfr177	UTSW	16	58872770	missense	probably damaging	1.00
R0975:Olfr177	UTSW	16	58873150	splice site	probably null
R1037:Olfr177	UTSW	16	58872970	missense	probably damaging	1.00
R1256:Olfr177	UTSW	16	58872843	nonsense	probably null
R1278:Olfr177	UTSW	16	58872977	missense	probably damaging	1.00
R1538:Olfr177	UTSW	16	58872898	missense	probably damaging	1.00
R1992:Olfr177	UTSW	16	58872511	missense	probably benign	0.43
R2173:Olfr177	UTSW	16	58872619	missense	probably damaging	0.99
R2392:Olfr177	UTSW	16	58872434	missense	probably damaging	1.00
R5651:Olfr177	UTSW	16	58872484	missense	probably damaging	0.99
R5652:Olfr177	UTSW	16	58872484	missense	probably damaging	0.99
R5653:Olfr177	UTSW	16	58872484	missense	probably damaging	0.99
R8031:Olfr177	UTSW	16	58872691	missense	probably benign	0.03
R8108:Olfr177	UTSW	16	58872236	missense	probably benign
R8531:Olfr177	UTSW	16	58872653	missense	probably damaging	1.00
R8833:Olfr177	UTSW	16	58872596	missense	probably damaging	0.99
R9150:Olfr177	UTSW	16	58872642	nonsense	probably null
R9318:Olfr177	UTSW	16	58872385	missense	probably damaging	1.00
R9389:Olfr177	UTSW	16	58872613	missense	probably damaging	1.00
V8831:Olfr177	UTSW	16	58873075	missense	probably benign	0.28

Predicted Primers

PCR Primer
(F):5'- TTGAAGGCAGAGCTTCTTGGACATC -3'
(R):5'- GAGGACAACTACTCCTTGGCAACAG -3'

Sequencing Primer
(F):5'- AGCTTCTTGGACATCATGCTG -3'
(R):5'- TGGCAACAGAGTTCATCCTCATAG -3'

Posted On

2013-05-09