Mutagenetix > Incidental Mutation

Incidental Mutation 'R0362:Mdn1'

36221

Institutional Source

Beutler Lab

Gene Symbol

Mdn1

Ensembl Gene

ENSMUSG00000058006

Gene Name

midasin AAA ATPase 1

Synonyms

4833432B22Rik, LOC213784, D4Abb1e

MMRRC Submission

038568-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0362 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

32657119-32775217 bp(+) (GRCm38)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

A to T at 32746439 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000136222 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071642] [ENSMUST00000071642] [ENSMUST00000178134] [ENSMUST00000178134]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000071642

SMART Domains

Protein: ENSMUSP00000071569
Gene: ENSMUSG00000058006

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	7.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4672	4681	N/A	INTRINSIC
low complexity region	4735	4756	N/A	INTRINSIC
low complexity region	4769	4790	N/A	INTRINSIC
low complexity region	4886	4905	N/A	INTRINSIC
low complexity region	4924	4937	N/A	INTRINSIC
coiled coil region	4957	4983	N/A	INTRINSIC
low complexity region	5000	5017	N/A	INTRINSIC
low complexity region	5176	5192	N/A	INTRINSIC
low complexity region	5315	5329	N/A	INTRINSIC
VWA	5375	5556	2.73e-6	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000071642

SMART Domains

Protein: ENSMUSP00000071569
Gene: ENSMUSG00000058006

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	7.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4672	4681	N/A	INTRINSIC
low complexity region	4735	4756	N/A	INTRINSIC
low complexity region	4769	4790	N/A	INTRINSIC
low complexity region	4886	4905	N/A	INTRINSIC
low complexity region	4924	4937	N/A	INTRINSIC
coiled coil region	4957	4983	N/A	INTRINSIC
low complexity region	5000	5017	N/A	INTRINSIC
low complexity region	5176	5192	N/A	INTRINSIC
low complexity region	5315	5329	N/A	INTRINSIC
VWA	5375	5556	2.73e-6	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125323

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000138577

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149941

Predicted Effect

probably null
Transcript: ENSMUST00000178134

SMART Domains

Protein: ENSMUSP00000136222
Gene: ENSMUSG00000058006

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	4.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4667	4676	N/A	INTRINSIC
low complexity region	4730	4751	N/A	INTRINSIC
low complexity region	4764	4785	N/A	INTRINSIC
low complexity region	4881	4900	N/A	INTRINSIC
low complexity region	4919	4932	N/A	INTRINSIC
coiled coil region	4952	4978	N/A	INTRINSIC
low complexity region	4992	5010	N/A	INTRINSIC
low complexity region	5169	5185	N/A	INTRINSIC
low complexity region	5308	5322	N/A	INTRINSIC
VWA	5368	5549	2.73e-6	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000178134

SMART Domains

Protein: ENSMUSP00000136222
Gene: ENSMUSG00000058006

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	4.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4667	4676	N/A	INTRINSIC
low complexity region	4730	4751	N/A	INTRINSIC
low complexity region	4764	4785	N/A	INTRINSIC
low complexity region	4881	4900	N/A	INTRINSIC
low complexity region	4919	4932	N/A	INTRINSIC
coiled coil region	4952	4978	N/A	INTRINSIC
low complexity region	4992	5010	N/A	INTRINSIC
low complexity region	5169	5185	N/A	INTRINSIC
low complexity region	5308	5322	N/A	INTRINSIC
VWA	5368	5549	2.73e-6	SMART

Meta Mutation Damage Score

0.9487

Coding Region Coverage

1x: 99.4%
3x: 98.6%
10x: 96.8%
20x: 94.0%

Validation Efficiency

99% (104/105)

Allele List at MGI

All alleles(29) : Targeted, other(2) Gene trapped(27)

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932414N04Rik	A	G	2: 68,732,917 (GRCm38)	Q401R	probably benign	Het
Acpp	A	G	9: 104,314,427 (GRCm38)	F220S	probably damaging	Het
Adam7	A	G	14: 68,509,656 (GRCm38)		probably benign	Het
Adamts6	A	G	13: 104,390,076 (GRCm38)		probably null	Het
Ascc3	T	C	10: 50,748,955 (GRCm38)		probably benign	Het
Atg10	T	C	13: 91,040,990 (GRCm38)		probably null	Het
Atm	T	C	9: 53,458,838 (GRCm38)	I2325V	possibly damaging	Het
Btnl9	C	T	11: 49,169,616 (GRCm38)	R435H	possibly damaging	Het
Ccdc180	A	G	4: 45,923,551 (GRCm38)	K1111E	probably damaging	Het
Col11a2	T	A	17: 34,062,446 (GRCm38)		probably null	Het
Ctcfl	A	G	2: 173,118,443 (GRCm38)	W116R	probably damaging	Het
Ctsk	A	T	3: 95,500,944 (GRCm38)	Y37F	probably damaging	Het
Daam2	G	C	17: 49,480,785 (GRCm38)		probably null	Het
Dcdc2b	T	C	4: 129,610,238 (GRCm38)		probably null	Het
Ddx28	C	T	8: 106,011,294 (GRCm38)	R44Q	probably damaging	Het
Dhx29	T	A	13: 112,962,859 (GRCm38)	N1139K	probably benign	Het
Dnah17	A	T	11: 118,098,539 (GRCm38)	M1281K	probably benign	Het
Dnah6	T	C	6: 73,208,609 (GRCm38)	S110G	probably benign	Het
Drc7	T	C	8: 95,072,855 (GRCm38)	Y553H	probably benign	Het
Dync2h1	T	C	9: 7,005,487 (GRCm38)		probably null	Het
Ecm1	A	G	3: 95,737,057 (GRCm38)	I152T	possibly damaging	Het
Edc4	C	G	8: 105,886,775 (GRCm38)	P307R	probably damaging	Het
Egr1	A	G	18: 34,863,313 (GRCm38)	T383A	possibly damaging	Het
Eml2	A	G	7: 19,190,806 (GRCm38)		probably null	Het
Eno4	A	G	19: 58,943,624 (GRCm38)		probably benign	Het
Erbb4	A	T	1: 68,330,270 (GRCm38)	I404K	probably damaging	Het
Exoc7	G	T	11: 116,295,662 (GRCm38)	T310K	probably benign	Het
Fam102b	C	T	3: 108,980,181 (GRCm38)	E256K	probably benign	Het
Fam83e	G	T	7: 45,726,969 (GRCm38)	V369L	probably benign	Het
Fam96b	T	C	8: 104,641,590 (GRCm38)	D34G	probably null	Het
Fancc	A	T	13: 63,398,156 (GRCm38)	I91K	possibly damaging	Het
Fbn1	T	C	2: 125,309,777 (GRCm38)	Q2519R	probably damaging	Het
Fhod3	T	A	18: 25,090,076 (GRCm38)	C826*	probably null	Het
Foxi3	A	G	6: 70,956,628 (GRCm38)	D33G	probably benign	Het
Gcn1l1	T	C	5: 115,576,108 (GRCm38)		probably benign	Het
Gm14221	T	C	2: 160,568,390 (GRCm38)		noncoding transcript	Het
Golga4	T	A	9: 118,555,785 (GRCm38)	H630Q	probably benign	Het
Gpat4	T	C	8: 23,180,933 (GRCm38)	S88G	probably benign	Het
Gucy2d	A	T	7: 98,443,685 (GRCm38)	S90C	probably damaging	Het
Has2	A	C	15: 56,681,661 (GRCm38)	C182G	probably damaging	Het
Heatr5a	A	T	12: 51,888,861 (GRCm38)	S1647R	probably damaging	Het
Ifi35	T	C	11: 101,457,212 (GRCm38)	V48A	probably benign	Het
Lig1	T	A	7: 13,296,804 (GRCm38)		probably benign	Het
Magi2	A	G	5: 19,227,575 (GRCm38)	K96R	probably damaging	Het
Map7d1	G	T	4: 126,234,994 (GRCm38)	P462Q	probably damaging	Het
Mfsd4a	A	T	1: 132,059,275 (GRCm38)	V105E	probably damaging	Het
Mrpl53	C	T	6: 83,109,545 (GRCm38)	R77C	probably damaging	Het
Mtnr1b	C	T	9: 15,874,304 (GRCm38)	V53M	probably damaging	Het
Myo9b	T	C	8: 71,347,770 (GRCm38)	W990R	probably damaging	Het
Myt1	A	T	2: 181,763,393 (GRCm38)		probably benign	Het
Nf1	C	T	11: 79,536,878 (GRCm38)	A1766V	probably damaging	Het
Nlrp3	T	C	11: 59,548,797 (GRCm38)	V400A	possibly damaging	Het
Nup205	T	A	6: 35,196,714 (GRCm38)		probably null	Het
Nxf1	T	C	19: 8,764,151 (GRCm38)		probably null	Het
Olfr1352	A	T	10: 78,984,386 (GRCm38)	M199L	probably benign	Het
P4hb	T	C	11: 120,563,336 (GRCm38)	K311E	probably benign	Het
Pafah1b1	T	C	11: 74,683,631 (GRCm38)	N243S	probably benign	Het
Parp8	G	A	13: 116,924,968 (GRCm38)	Q141*	probably null	Het
Pkd2l2	A	C	18: 34,435,327 (GRCm38)	D543A	probably benign	Het
Pld5	A	T	1: 175,975,580 (GRCm38)	L311*	probably null	Het
Plekha5	G	A	6: 140,591,747 (GRCm38)	R646K	possibly damaging	Het
Plpp5	A	T	8: 25,724,192 (GRCm38)	T144S	probably benign	Het
Ppp6r3	A	G	19: 3,478,285 (GRCm38)	L542S	probably damaging	Het
Prkar2b	A	T	12: 31,987,974 (GRCm38)		probably null	Het
Psmg1	A	T	16: 95,987,971 (GRCm38)	S129T	possibly damaging	Het
Radil	T	C	5: 142,543,827 (GRCm38)	D38G	probably benign	Het
Ric1	T	C	19: 29,601,011 (GRCm38)		probably null	Het
Rp1l1	T	A	14: 64,031,066 (GRCm38)	L1367*	probably null	Het
Rxfp1	A	T	3: 79,737,793 (GRCm38)	M1K	probably null	Het
Serpina6	G	T	12: 103,651,949 (GRCm38)	L202I	probably damaging	Het
Simc1	T	C	13: 54,528,467 (GRCm38)	I98T	probably damaging	Het
Slc17a6	A	G	7: 51,658,771 (GRCm38)	Y281C	probably damaging	Het
Slc26a11	T	A	11: 119,379,941 (GRCm38)		probably benign	Het
Slc34a1	T	A	13: 55,402,898 (GRCm38)		probably null	Het
Slfn10-ps	T	A	11: 83,035,774 (GRCm38)		noncoding transcript	Het
Sohlh2	A	G	3: 55,207,742 (GRCm38)	N383D	probably damaging	Het
Spag6	T	A	2: 18,710,491 (GRCm38)	L27H	probably damaging	Het
Sptlc3	A	G	2: 139,546,555 (GRCm38)		probably benign	Het
St3gal4	T	A	9: 35,053,173 (GRCm38)	K199*	probably null	Het
Stat5a	T	A	11: 100,882,083 (GRCm38)	D712E	probably benign	Het
Stmn2	A	T	3: 8,545,690 (GRCm38)	D78V	probably damaging	Het
Stpg1	C	T	4: 135,506,466 (GRCm38)	P20S	possibly damaging	Het
Taf2	A	T	15: 55,045,929 (GRCm38)	V640E	probably damaging	Het
Tbce	T	C	13: 13,998,162 (GRCm38)	E501G	probably benign	Het
Tecpr2	A	G	12: 110,968,940 (GRCm38)	S1398G	probably damaging	Het
Tenm4	T	A	7: 96,772,035 (GRCm38)	Y598*	probably null	Het
Ticrr	A	G	7: 79,677,340 (GRCm38)	S599G	probably damaging	Het
Tnc	A	C	4: 64,017,442 (GRCm38)	V419G	probably damaging	Het
Trappc1	C	A	11: 69,325,576 (GRCm38)	P110T	probably benign	Het
Trbv12-2	C	T	6: 41,119,059 (GRCm38)		probably benign	Het
Ttbk2	A	T	2: 120,745,783 (GRCm38)	N835K	possibly damaging	Het
Tubgcp5	A	G	7: 55,800,684 (GRCm38)	D181G	probably damaging	Het
Tut1	T	C	19: 8,955,527 (GRCm38)	Y75H	possibly damaging	Het
Ulk2	C	A	11: 61,787,586 (GRCm38)	C769F	probably benign	Het
Vdac1	T	C	11: 52,374,973 (GRCm38)		probably benign	Het
Vmn2r124	T	C	17: 18,064,224 (GRCm38)		probably null	Het
Vps8	T	C	16: 21,608,227 (GRCm38)		probably benign	Het
Wdr35	T	C	12: 8,995,625 (GRCm38)		probably benign	Het
Zdhhc7	T	A	8: 120,086,647 (GRCm38)	E141V	probably null	Het
Zfp12	C	A	5: 143,245,223 (GRCm38)	S435Y	probably damaging	Het
Zfp974	A	T	7: 27,927,394 (GRCm38)		probably benign	Het
Zfyve9	T	A	4: 108,680,969 (GRCm38)	K1033N	probably damaging	Het
Zswim8	T	A	14: 20,721,945 (GRCm38)	S1572T	possibly damaging	Het

Other mutations in Mdn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Mdn1	APN	4	32,723,651 (GRCm38)	missense	probably damaging	1.00
IGL00426:Mdn1	APN	4	32,719,214 (GRCm38)	missense	possibly damaging	0.91
IGL00570:Mdn1	APN	4	32,735,719 (GRCm38)	missense	probably benign
IGL00573:Mdn1	APN	4	32,666,619 (GRCm38)	critical splice donor site	probably null
IGL00983:Mdn1	APN	4	32,735,525 (GRCm38)	missense	probably damaging	1.00
IGL01288:Mdn1	APN	4	32,730,864 (GRCm38)	missense	probably benign	0.00
IGL01359:Mdn1	APN	4	32,743,686 (GRCm38)	missense	probably benign	0.10
IGL01457:Mdn1	APN	4	32,715,922 (GRCm38)	missense	possibly damaging	0.82
IGL01530:Mdn1	APN	4	32,711,938 (GRCm38)	splice site	probably benign
IGL01684:Mdn1	APN	4	32,726,857 (GRCm38)	missense	probably benign
IGL01753:Mdn1	APN	4	32,708,483 (GRCm38)	missense	probably benign
IGL01901:Mdn1	APN	4	32,669,591 (GRCm38)	missense	probably damaging	1.00
IGL01952:Mdn1	APN	4	32,723,657 (GRCm38)	missense	possibly damaging	0.82
IGL01960:Mdn1	APN	4	32,758,393 (GRCm38)	missense	probably benign	0.14
IGL02019:Mdn1	APN	4	32,749,948 (GRCm38)	missense	possibly damaging	0.93
IGL02100:Mdn1	APN	4	32,715,708 (GRCm38)	missense	possibly damaging	0.90
IGL02117:Mdn1	APN	4	32,709,364 (GRCm38)	missense	probably benign	0.00
IGL02154:Mdn1	APN	4	32,740,395 (GRCm38)	missense	probably benign	0.35
IGL02216:Mdn1	APN	4	32,739,092 (GRCm38)	missense	probably benign	0.03
IGL02371:Mdn1	APN	4	32,676,860 (GRCm38)	splice site	probably benign
IGL02396:Mdn1	APN	4	32,700,120 (GRCm38)	missense	probably damaging	0.99
IGL02454:Mdn1	APN	4	32,694,674 (GRCm38)	critical splice donor site	probably null
IGL02502:Mdn1	APN	4	32,670,579 (GRCm38)	missense	possibly damaging	0.69
IGL02883:Mdn1	APN	4	32,763,199 (GRCm38)	missense	probably benign	0.05
IGL02946:Mdn1	APN	4	32,734,366 (GRCm38)	missense	probably damaging	0.98
IGL02950:Mdn1	APN	4	32,713,360 (GRCm38)	splice site	probably benign
IGL03076:Mdn1	APN	4	32,735,564 (GRCm38)	missense	probably damaging	0.97
IGL03129:Mdn1	APN	4	32,729,994 (GRCm38)	missense	possibly damaging	0.47
IGL03234:Mdn1	APN	4	32,732,842 (GRCm38)	missense	probably benign	0.06
3-1:Mdn1	UTSW	4	32,725,967 (GRCm38)	critical splice donor site	probably null
IGL03046:Mdn1	UTSW	4	32,694,495 (GRCm38)	missense	possibly damaging	0.73
P0035:Mdn1	UTSW	4	32,749,934 (GRCm38)	missense	probably benign	0.05
PIT4508001:Mdn1	UTSW	4	32,719,223 (GRCm38)	missense	probably damaging	0.97
PIT4618001:Mdn1	UTSW	4	32,746,527 (GRCm38)	missense	probably benign	0.20
R0008:Mdn1	UTSW	4	32,718,317 (GRCm38)	missense	possibly damaging	0.47
R0110:Mdn1	UTSW	4	32,738,619 (GRCm38)	missense	probably benign	0.20
R0125:Mdn1	UTSW	4	32,729,956 (GRCm38)	missense	probably damaging	0.98
R0257:Mdn1	UTSW	4	32,693,534 (GRCm38)	missense	probably damaging	0.99
R0266:Mdn1	UTSW	4	32,741,835 (GRCm38)	missense	probably damaging	0.99
R0349:Mdn1	UTSW	4	32,750,318 (GRCm38)	missense	probably damaging	1.00
R0421:Mdn1	UTSW	4	32,684,707 (GRCm38)	missense	probably benign	0.39
R0450:Mdn1	UTSW	4	32,738,619 (GRCm38)	missense	probably benign	0.20
R0465:Mdn1	UTSW	4	32,699,204 (GRCm38)	splice site	probably benign
R0469:Mdn1	UTSW	4	32,738,619 (GRCm38)	missense	probably benign	0.20
R0477:Mdn1	UTSW	4	32,750,928 (GRCm38)	missense	probably benign	0.02
R0481:Mdn1	UTSW	4	32,767,182 (GRCm38)	splice site	probably benign
R0504:Mdn1	UTSW	4	32,698,916 (GRCm38)	splice site	probably benign
R0522:Mdn1	UTSW	4	32,672,837 (GRCm38)	missense	probably benign	0.09
R0550:Mdn1	UTSW	4	32,730,479 (GRCm38)	missense	probably benign	0.13
R0607:Mdn1	UTSW	4	32,732,829 (GRCm38)	missense	probably benign	0.36
R0607:Mdn1	UTSW	4	32,712,014 (GRCm38)	missense	probably damaging	1.00
R0664:Mdn1	UTSW	4	32,768,011 (GRCm38)	nonsense	probably null
R0701:Mdn1	UTSW	4	32,699,263 (GRCm38)	missense	probably benign	0.00
R0801:Mdn1	UTSW	4	32,668,895 (GRCm38)	missense	probably benign	0.04
R0841:Mdn1	UTSW	4	32,752,032 (GRCm38)	missense	probably benign	0.23
R0849:Mdn1	UTSW	4	32,741,835 (GRCm38)	missense	probably damaging	0.99
R0893:Mdn1	UTSW	4	32,701,713 (GRCm38)	missense	probably benign	0.01
R1114:Mdn1	UTSW	4	32,746,568 (GRCm38)	critical splice donor site	probably null
R1137:Mdn1	UTSW	4	32,694,511 (GRCm38)	missense	probably damaging	1.00
R1185:Mdn1	UTSW	4	32,735,576 (GRCm38)	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32,735,576 (GRCm38)	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32,735,576 (GRCm38)	missense	possibly damaging	0.94
R1257:Mdn1	UTSW	4	32,667,089 (GRCm38)	critical splice acceptor site	probably null
R1356:Mdn1	UTSW	4	32,700,334 (GRCm38)	splice site	probably benign
R1466:Mdn1	UTSW	4	32,730,788 (GRCm38)	missense	probably benign	0.28
R1466:Mdn1	UTSW	4	32,730,788 (GRCm38)	missense	probably benign	0.28
R1518:Mdn1	UTSW	4	32,739,977 (GRCm38)	missense	probably damaging	1.00
R1569:Mdn1	UTSW	4	32,723,501 (GRCm38)	missense	probably null	0.10
R1574:Mdn1	UTSW	4	32,722,315 (GRCm38)	missense	probably benign
R1574:Mdn1	UTSW	4	32,722,315 (GRCm38)	missense	probably benign
R1591:Mdn1	UTSW	4	32,700,092 (GRCm38)	missense	possibly damaging	0.65
R1678:Mdn1	UTSW	4	32,663,050 (GRCm38)	missense	probably damaging	0.99
R1696:Mdn1	UTSW	4	32,700,417 (GRCm38)	missense	possibly damaging	0.91
R1707:Mdn1	UTSW	4	32,693,504 (GRCm38)	missense	probably damaging	1.00
R1749:Mdn1	UTSW	4	32,773,952 (GRCm38)	missense	probably damaging	1.00
R1780:Mdn1	UTSW	4	32,700,103 (GRCm38)	missense	probably damaging	1.00
R1833:Mdn1	UTSW	4	32,720,761 (GRCm38)	missense	probably damaging	0.97
R1858:Mdn1	UTSW	4	32,730,881 (GRCm38)	missense	probably benign	0.17
R1870:Mdn1	UTSW	4	32,763,339 (GRCm38)	missense	probably damaging	1.00
R1887:Mdn1	UTSW	4	32,742,540 (GRCm38)	missense	probably damaging	1.00
R1909:Mdn1	UTSW	4	32,760,839 (GRCm38)	small deletion	probably benign
R2075:Mdn1	UTSW	4	32,716,058 (GRCm38)	missense	probably benign	0.03
R2103:Mdn1	UTSW	4	32,738,712 (GRCm38)	missense	possibly damaging	0.75
R2104:Mdn1	UTSW	4	32,743,843 (GRCm38)	splice site	probably null
R2110:Mdn1	UTSW	4	32,700,409 (GRCm38)	missense	probably damaging	1.00
R2111:Mdn1	UTSW	4	32,700,409 (GRCm38)	missense	probably damaging	1.00
R2206:Mdn1	UTSW	4	32,716,271 (GRCm38)	missense	possibly damaging	0.71
R2221:Mdn1	UTSW	4	32,763,306 (GRCm38)	missense	probably benign	0.37
R2240:Mdn1	UTSW	4	32,765,701 (GRCm38)	missense	possibly damaging	0.90
R2351:Mdn1	UTSW	4	32,750,010 (GRCm38)	missense	probably benign	0.21
R2421:Mdn1	UTSW	4	32,723,621 (GRCm38)	missense	probably damaging	0.96
R3036:Mdn1	UTSW	4	32,750,013 (GRCm38)	missense	probably damaging	0.99
R3434:Mdn1	UTSW	4	32,733,726 (GRCm38)	critical splice donor site	probably null
R3435:Mdn1	UTSW	4	32,733,726 (GRCm38)	critical splice donor site	probably null
R3783:Mdn1	UTSW	4	32,720,818 (GRCm38)	missense	probably benign	0.01
R3811:Mdn1	UTSW	4	32,693,506 (GRCm38)	nonsense	probably null
R3973:Mdn1	UTSW	4	32,722,363 (GRCm38)	missense	probably benign	0.00
R4154:Mdn1	UTSW	4	32,707,475 (GRCm38)	missense	probably damaging	0.96
R4372:Mdn1	UTSW	4	32,743,809 (GRCm38)	missense	probably benign	0.03
R4393:Mdn1	UTSW	4	32,754,482 (GRCm38)	missense	possibly damaging	0.48
R4438:Mdn1	UTSW	4	32,704,635 (GRCm38)	missense	probably damaging	1.00
R4471:Mdn1	UTSW	4	32,668,860 (GRCm38)	missense	probably benign	0.00
R4509:Mdn1	UTSW	4	32,715,883 (GRCm38)	missense	probably damaging	1.00
R4538:Mdn1	UTSW	4	32,722,334 (GRCm38)	missense	probably damaging	1.00
R4557:Mdn1	UTSW	4	32,754,437 (GRCm38)	missense	probably damaging	1.00
R4570:Mdn1	UTSW	4	32,741,812 (GRCm38)	missense	probably damaging	1.00
R4591:Mdn1	UTSW	4	32,707,636 (GRCm38)	missense	probably damaging	1.00
R4658:Mdn1	UTSW	4	32,730,749 (GRCm38)	splice site	probably null
R4667:Mdn1	UTSW	4	32,679,572 (GRCm38)	missense	probably damaging	1.00
R4684:Mdn1	UTSW	4	32,666,430 (GRCm38)	missense	probably damaging	1.00
R4778:Mdn1	UTSW	4	32,683,583 (GRCm38)	nonsense	probably null
R4807:Mdn1	UTSW	4	32,685,651 (GRCm38)	splice site	probably null
R4923:Mdn1	UTSW	4	32,671,608 (GRCm38)	missense	possibly damaging	0.89
R4951:Mdn1	UTSW	4	32,707,459 (GRCm38)	missense	probably damaging	1.00
R4963:Mdn1	UTSW	4	32,756,512 (GRCm38)	missense	probably benign	0.00
R4971:Mdn1	UTSW	4	32,739,827 (GRCm38)	missense	probably damaging	1.00
R4973:Mdn1	UTSW	4	32,734,418 (GRCm38)	missense	probably benign	0.01
R5122:Mdn1	UTSW	4	32,670,593 (GRCm38)	missense	probably damaging	1.00
R5159:Mdn1	UTSW	4	32,774,008 (GRCm38)	missense	possibly damaging	0.93
R5164:Mdn1	UTSW	4	32,759,011 (GRCm38)	splice site	probably null
R5215:Mdn1	UTSW	4	32,741,418 (GRCm38)	missense	possibly damaging	0.78
R5217:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5219:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5365:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5366:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5368:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5445:Mdn1	UTSW	4	32,723,690 (GRCm38)	missense	probably damaging	0.98
R5462:Mdn1	UTSW	4	32,720,897 (GRCm38)	missense	probably benign
R5522:Mdn1	UTSW	4	32,685,783 (GRCm38)	missense	probably damaging	1.00
R5525:Mdn1	UTSW	4	32,767,961 (GRCm38)	missense	possibly damaging	0.73
R5578:Mdn1	UTSW	4	32,728,167 (GRCm38)	missense	probably benign	0.04
R5605:Mdn1	UTSW	4	32,765,664 (GRCm38)	missense	probably benign
R5621:Mdn1	UTSW	4	32,716,371 (GRCm38)	missense	possibly damaging	0.46
R5636:Mdn1	UTSW	4	32,695,480 (GRCm38)	missense	probably damaging	1.00
R5650:Mdn1	UTSW	4	32,667,467 (GRCm38)	splice site	probably null
R5780:Mdn1	UTSW	4	32,722,950 (GRCm38)	missense	probably benign	0.02
R5838:Mdn1	UTSW	4	32,754,547 (GRCm38)	missense	probably damaging	0.99
R5857:Mdn1	UTSW	4	32,670,646 (GRCm38)	missense	probably benign	0.09
R5895:Mdn1	UTSW	4	32,695,400 (GRCm38)	missense	probably damaging	1.00
R5943:Mdn1	UTSW	4	32,678,330 (GRCm38)	missense	probably damaging	1.00
R6008:Mdn1	UTSW	4	32,741,073 (GRCm38)	missense	probably damaging	1.00
R6013:Mdn1	UTSW	4	32,715,713 (GRCm38)	missense	probably damaging	1.00
R6075:Mdn1	UTSW	4	32,689,581 (GRCm38)	missense	possibly damaging	0.48
R6151:Mdn1	UTSW	4	32,684,735 (GRCm38)	missense	probably damaging	1.00
R6163:Mdn1	UTSW	4	32,716,040 (GRCm38)	missense	probably damaging	1.00
R6181:Mdn1	UTSW	4	32,715,953 (GRCm38)	missense	probably damaging	1.00
R6211:Mdn1	UTSW	4	32,696,269 (GRCm38)	missense	probably benign	0.12
R6249:Mdn1	UTSW	4	32,708,484 (GRCm38)	missense	possibly damaging	0.85
R6251:Mdn1	UTSW	4	32,748,590 (GRCm38)	missense	probably benign	0.13
R6253:Mdn1	UTSW	4	32,749,593 (GRCm38)	missense	probably benign	0.25
R6273:Mdn1	UTSW	4	32,715,979 (GRCm38)	missense	probably benign	0.01
R6297:Mdn1	UTSW	4	32,730,054 (GRCm38)	nonsense	probably null
R6384:Mdn1	UTSW	4	32,670,607 (GRCm38)	missense	probably damaging	1.00
R6463:Mdn1	UTSW	4	32,773,308 (GRCm38)	missense	probably damaging	1.00
R6528:Mdn1	UTSW	4	32,713,780 (GRCm38)	missense	probably damaging	1.00
R6688:Mdn1	UTSW	4	32,774,041 (GRCm38)	missense	possibly damaging	0.74
R6762:Mdn1	UTSW	4	32,676,786 (GRCm38)	missense	possibly damaging	0.50
R6794:Mdn1	UTSW	4	32,741,893 (GRCm38)	missense	probably damaging	1.00
R6894:Mdn1	UTSW	4	32,748,614 (GRCm38)	missense	possibly damaging	0.75
R6935:Mdn1	UTSW	4	32,774,041 (GRCm38)	missense	possibly damaging	0.74
R6980:Mdn1	UTSW	4	32,726,942 (GRCm38)	critical splice donor site	probably null
R6995:Mdn1	UTSW	4	32,733,374 (GRCm38)	missense	probably benign	0.03
R7048:Mdn1	UTSW	4	32,767,969 (GRCm38)	missense	probably benign	0.00
R7082:Mdn1	UTSW	4	32,762,341 (GRCm38)	missense	probably benign
R7158:Mdn1	UTSW	4	32,725,121 (GRCm38)	missense	probably benign	0.09
R7166:Mdn1	UTSW	4	32,746,446 (GRCm38)	missense	probably damaging	1.00
R7168:Mdn1	UTSW	4	32,719,184 (GRCm38)	missense	probably damaging	1.00
R7175:Mdn1	UTSW	4	32,694,634 (GRCm38)	missense	probably damaging	1.00
R7195:Mdn1	UTSW	4	32,701,823 (GRCm38)	missense	probably damaging	1.00
R7250:Mdn1	UTSW	4	32,695,427 (GRCm38)	missense	probably damaging	1.00
R7274:Mdn1	UTSW	4	32,725,944 (GRCm38)	missense	probably benign	0.12
R7330:Mdn1	UTSW	4	32,723,685 (GRCm38)	missense	probably benign	0.16
R7363:Mdn1	UTSW	4	32,691,729 (GRCm38)	missense	probably damaging	0.99
R7369:Mdn1	UTSW	4	32,773,375 (GRCm38)	missense	probably damaging	0.99
R7452:Mdn1	UTSW	4	32,739,030 (GRCm38)	missense	possibly damaging	0.87
R7523:Mdn1	UTSW	4	32,667,270 (GRCm38)	critical splice acceptor site	probably null
R7594:Mdn1	UTSW	4	32,696,359 (GRCm38)	missense	probably benign	0.27
R7605:Mdn1	UTSW	4	32,694,599 (GRCm38)	missense	probably damaging	1.00
R7661:Mdn1	UTSW	4	32,691,229 (GRCm38)	missense	probably benign	0.08
R7689:Mdn1	UTSW	4	32,739,912 (GRCm38)	missense	probably damaging	1.00
R7699:Mdn1	UTSW	4	32,741,344 (GRCm38)	missense	probably damaging	1.00
R7700:Mdn1	UTSW	4	32,741,344 (GRCm38)	missense	probably damaging	1.00
R7714:Mdn1	UTSW	4	32,722,360 (GRCm38)	missense	possibly damaging	0.75
R7718:Mdn1	UTSW	4	32,718,420 (GRCm38)	missense	probably damaging	1.00
R7762:Mdn1	UTSW	4	32,734,421 (GRCm38)	missense	probably benign
R7787:Mdn1	UTSW	4	32,741,794 (GRCm38)	missense	probably damaging	1.00
R8111:Mdn1	UTSW	4	32,674,003 (GRCm38)	missense	possibly damaging	0.81
R8222:Mdn1	UTSW	4	32,707,477 (GRCm38)	missense	probably benign	0.09
R8246:Mdn1	UTSW	4	32,657,284 (GRCm38)	missense	probably benign	0.06
R8267:Mdn1	UTSW	4	32,742,485 (GRCm38)	missense	possibly damaging	0.82
R8286:Mdn1	UTSW	4	32,731,960 (GRCm38)	missense	possibly damaging	0.91
R8305:Mdn1	UTSW	4	32,725,107 (GRCm38)	missense	probably benign
R8318:Mdn1	UTSW	4	32,735,897 (GRCm38)	critical splice donor site	probably null
R8379:Mdn1	UTSW	4	32,756,453 (GRCm38)	missense	probably null	1.00
R8384:Mdn1	UTSW	4	32,765,680 (GRCm38)	missense	probably benign	0.05
R8514:Mdn1	UTSW	4	32,739,857 (GRCm38)	missense	probably damaging	1.00
R8560:Mdn1	UTSW	4	32,743,830 (GRCm38)	missense	probably benign	0.08
R8672:Mdn1	UTSW	4	32,768,793 (GRCm38)	missense	probably damaging	1.00
R8708:Mdn1	UTSW	4	32,725,854 (GRCm38)	missense	probably damaging	1.00
R8769:Mdn1	UTSW	4	32,751,390 (GRCm38)	missense	probably damaging	0.97
R8896:Mdn1	UTSW	4	32,678,328 (GRCm38)	missense	probably benign	0.28
R8918:Mdn1	UTSW	4	32,744,579 (GRCm38)	nonsense	probably null
R8920:Mdn1	UTSW	4	32,719,280 (GRCm38)	missense	probably damaging	1.00
R8966:Mdn1	UTSW	4	32,672,837 (GRCm38)	nonsense	probably null
R8997:Mdn1	UTSW	4	32,773,275 (GRCm38)	missense	probably damaging	1.00
R9120:Mdn1	UTSW	4	32,701,814 (GRCm38)	missense	probably damaging	1.00
R9129:Mdn1	UTSW	4	32,676,812 (GRCm38)	missense	probably benign	0.24
R9131:Mdn1	UTSW	4	32,762,275 (GRCm38)	missense	possibly damaging	0.69
R9200:Mdn1	UTSW	4	32,760,791 (GRCm38)	missense	probably benign	0.00
R9226:Mdn1	UTSW	4	32,694,612 (GRCm38)	missense	probably benign	0.25
R9235:Mdn1	UTSW	4	32,739,122 (GRCm38)	missense	probably benign	0.10
R9293:Mdn1	UTSW	4	32,707,579 (GRCm38)	missense	probably damaging	1.00
R9315:Mdn1	UTSW	4	32,760,911 (GRCm38)	missense	probably benign	0.00
R9338:Mdn1	UTSW	4	32,666,536 (GRCm38)	missense	probably benign	0.00
R9353:Mdn1	UTSW	4	32,693,504 (GRCm38)	missense	probably damaging	1.00
R9393:Mdn1	UTSW	4	32,713,825 (GRCm38)	missense
R9420:Mdn1	UTSW	4	32,678,414 (GRCm38)	missense	probably damaging	1.00
R9475:Mdn1	UTSW	4	32,739,849 (GRCm38)	missense	possibly damaging	0.65
R9583:Mdn1	UTSW	4	32,741,372 (GRCm38)	missense	probably damaging	1.00
R9600:Mdn1	UTSW	4	32,684,723 (GRCm38)	nonsense	probably null
R9640:Mdn1	UTSW	4	32,754,539 (GRCm38)	missense	probably damaging	1.00
R9688:Mdn1	UTSW	4	32,745,590 (GRCm38)	missense	probably damaging	1.00
R9744:Mdn1	UTSW	4	32,715,711 (GRCm38)	missense	possibly damaging	0.91
X0066:Mdn1	UTSW	4	32,739,030 (GRCm38)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,696,244 (GRCm38)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,668,944 (GRCm38)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,667,102 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AGAACCACATCAGGGTCACTTTGAATC -3'
(R):5'- TGCTAGTCAGCAGTGAAGAACATACAC -3'

Sequencing Primer
(F):5'- gcctcacactcaagaccc -3'
(R):5'- AAGTGCCAGCCAGATCTTTTTG -3'

Posted On

2013-05-09