Mutagenetix > Incidental Mutations

Incidental Mutation 'IGL02932:Cry2'

364142

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Cry2

Ensembl Gene

ENSMUSG00000068742

Gene Name

cryptochrome 2 (photolyase-like)

Synonyms

Accession Numbers

Is this an essential gene?

Possibly essential (E-score: 0.559) question?

Stock #

IGL02932

Quality Score

Status

Chromosome

Chromosomal Location

92403646-92434043 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 92413117 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 460 (R460*)

Ref Sequence

ENSEMBL: ENSMUSP00000106909 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]

AlphaFold

Q9R194

Predicted Effect

probably null
Transcript: ENSMUST00000090559
AA Change: R460*

SMART Domains

Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742
AA Change: R460*

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	187	2.4e-50	PFAM
Pfam:FAD_binding_7	231	504	4.4e-89	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000111278
AA Change: R460*

SMART Domains

Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742
AA Change: R460*

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	189	3.6e-50	PFAM
Pfam:FAD_binding_7	230	506	1.4e-105	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126002

Coding Region Coverage

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgap42	T	A	9: 9,115,708	E122D	probably damaging	Het
Asb3	G	A	11: 31,029,067		probably null	Het
Avl9	T	C	6: 56,736,551	S265P	probably benign	Het
Cep44	C	T	8: 56,547,424	R18Q	probably damaging	Het
Clec10a	T	C	11: 70,169,728		probably benign	Het
Cog4	A	G	8: 110,852,433	I115V	probably benign	Het
Coq3	C	T	4: 21,900,430	A219V	probably benign	Het
Ddx39b	A	G	17: 35,253,361		probably benign	Het
Dhx40	G	A	11: 86,771,929	R646C	probably damaging	Het
Ears2	G	A	7: 122,063,061	R55C	probably damaging	Het
Eif3m	C	T	2: 105,014,869	G26R	probably damaging	Het
Exo5	A	G	4: 120,922,545	L41P	probably benign	Het
Fut10	A	G	8: 31,259,937	H417R	probably damaging	Het
Gars	T	A	6: 55,060,944	L296Q	probably damaging	Het
Gpr143	A	T	X: 152,793,443		probably benign	Het
Htr1b	C	A	9: 81,631,636	R306L	probably damaging	Het
Lama3	G	A	18: 12,528,801	A2185T	probably damaging	Het
Mbd5	C	A	2: 49,279,448	Q1544K	possibly damaging	Het
Mccc1	T	C	3: 35,960,029	E713G	possibly damaging	Het
Mttp	A	G	3: 138,111,744	F415S	probably benign	Het
Mug1	A	G	6: 121,887,427	T1428A	probably benign	Het
Mup6	A	C	4: 60,006,009	D159A	probably damaging	Het
Nsd2	T	C	5: 33,880,128	L698P	probably damaging	Het
Olfr1184	G	T	2: 88,487,175	V148F	probably benign	Het
Olfr1361	A	T	13: 21,658,831	M164K	probably damaging	Het
Olfr495	A	G	7: 108,395,513	N131S	probably benign	Het
Olfr815	A	T	10: 129,902,418	C97*	probably null	Het
Osgepl1	G	A	1: 53,321,516	R372H	probably benign	Het
Pias2	C	T	18: 77,145,103	H537Y	probably damaging	Het
Slc38a7	A	C	8: 95,846,155	I149M	probably damaging	Het
Smn1	A	G	13: 100,127,964	T68A	probably benign	Het
Syde2	A	G	3: 146,001,476	K657R	possibly damaging	Het
Tdrd5	A	T	1: 156,270,620	H625Q	possibly damaging	Het
Tead3	T	C	17: 28,341,351	Y2C	probably damaging	Het
Tmem255a	T	A	X: 38,208,063	T280S	probably benign	Het
Ttpa	A	G	4: 20,021,215	T128A	possibly damaging	Het
Wdhd1	A	G	14: 47,272,134		probably null	Het

Other mutations in Cry2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01995:Cry2	APN	2	92424632	missense	probably benign	0.15
IGL02167:Cry2	APN	2	92433821	missense	possibly damaging	0.93
IGL02183:Cry2	APN	2	92413039	missense	probably damaging	0.99
IGL02343:Cry2	APN	2	92426921	missense	possibly damaging	0.90
IGL02432:Cry2	APN	2	92413667	missense	probably damaging	0.99
IGL02725:Cry2	APN	2	92413260	splice site	probably benign
IGL03122:Cry2	APN	2	92413295	missense	probably damaging	1.00
IGL03366:Cry2	APN	2	92413715	missense	probably damaging	1.00
R0679:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R1325:Cry2	UTSW	2	92413770	missense	probably damaging	1.00
R1862:Cry2	UTSW	2	92424566	missense	probably damaging	1.00
R1891:Cry2	UTSW	2	92413640	missense	possibly damaging	0.93
R2189:Cry2	UTSW	2	92411692	missense	possibly damaging	0.84
R4032:Cry2	UTSW	2	92413827	missense	probably benign	0.00
R4689:Cry2	UTSW	2	92424554	missense	probably benign	0.38
R5130:Cry2	UTSW	2	92424599	missense	probably benign	0.28
R5145:Cry2	UTSW	2	92413060	missense	probably benign
R5970:Cry2	UTSW	2	92412967	missense	probably benign	0.08
R6179:Cry2	UTSW	2	92413842	missense	probably damaging	0.98
R7102:Cry2	UTSW	2	92413093	missense	probably damaging	0.99
R7158:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R7213:Cry2	UTSW	2	92413659	missense	probably benign	0.00
R7257:Cry2	UTSW	2	92412981	missense	possibly damaging	0.67
R7378:Cry2	UTSW	2	92413664	missense	probably damaging	1.00
R7427:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7428:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7440:Cry2	UTSW	2	92413638	missense	probably damaging	1.00
R7531:Cry2	UTSW	2	92413005	missense	probably damaging	0.98
R8234:Cry2	UTSW	2	92412629	missense	probably benign
R8350:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8450:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8496:Cry2	UTSW	2	92426939	missense	probably damaging	1.00
R9172:Cry2	UTSW	2	92413648	missense	probably damaging	1.00
R9283:Cry2	UTSW	2	92413904	missense	probably damaging	1.00

Posted On

2015-12-18