Mutagenetix > Incidental Mutation

Incidental Mutation 'R4767:Vmn2r86'

366230

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r86

Ensembl Gene

ENSMUSG00000092162

Gene Name

vomeronasal 2, receptor 86

Synonyms

EG625109

MMRRC Submission

042408-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R4767 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

130445707-130455894 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 130455737 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 53 (M53K)

Ref Sequence

ENSEMBL: ENSMUSP00000126596 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170257]

AlphaFold

G5E8Y4

Predicted Effect

probably benign
Transcript: ENSMUST00000170257
AA Change: M53K

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000126596
Gene: ENSMUSG00000092162
AA Change: M53K

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	1.1e-25	PFAM
Pfam:NCD3G	508	562	2.4e-19	PFAM
Pfam:7tm_3	595	829	6.4e-55	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

95% (62/65)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam19	T	C	11: 46,138,977		probably null	Het
Aig1	T	C	10: 13,801,858	N130S	probably damaging	Het
Alx1	A	T	10: 103,025,186	Y160*	probably null	Het
Ap4e1	T	C	2: 127,060,438	I755T	probably benign	Het
Apoc3	C	A	9: 46,234,535	E21*	probably null	Het
Atp9b	G	T	18: 80,753,070	H919Q	probably damaging	Het
Cemip	T	G	7: 83,973,306	Y555S	probably damaging	Het
Cic	T	C	7: 25,271,600	V252A	possibly damaging	Het
Cracr2a	A	T	6: 127,611,507	N210Y	probably damaging	Het
Crocc2	G	A	1: 93,202,856	R953Q	possibly damaging	Het
Csnk1d	A	G	11: 120,969,128	S318P	probably benign	Het
Ddx21	A	G	10: 62,591,972	L384P	probably damaging	Het
Dnah5	C	T	15: 28,270,474	T974I	probably benign	Het
Duox1	A	G	2: 122,333,441	Y863C	possibly damaging	Het
Epg5	C	A	18: 78,023,283	P2133T	possibly damaging	Het
Ephb6	A	T	6: 41,614,185	Q92L	possibly damaging	Het
Ercc4	C	A	16: 13,122,095	A73D	probably damaging	Het
Eva1c	A	T	16: 90,904,347	Y290F	probably damaging	Het
Galnt5	T	A	2: 58,028,144	V798E	possibly damaging	Het
Haus4	T	C	14: 54,548,885	E149G	probably damaging	Het
Ighv1-13	A	G	12: 114,630,936	Y86C	unknown	Het
Igkv9-120	G	T	6: 68,050,367	R88S	possibly damaging	Het
Lama3	T	C	18: 12,500,563	V1584A	probably benign	Het
Lamb1	A	G	12: 31,308,011	E1119G	probably damaging	Het
Lao1	T	C	4: 118,967,988	L335P	probably damaging	Het
Mindy4	A	G	6: 55,260,565	D375G	probably damaging	Het
Myo5a	T	C	9: 75,144,076	I317T	probably damaging	Het
Nhlrc2	G	A	19: 56,570,466	V128I	probably benign	Het
Nlrp2	C	T	7: 5,328,024	D458N	probably damaging	Het
Olfr107	T	A	17: 37,406,200	C217*	probably null	Het
Olfr1497	A	T	19: 13,795,045	C189S	probably damaging	Het
Olfr347	A	T	2: 36,734,323	M1L	probably benign	Het
Olfr76	G	T	19: 12,119,936	H247N	probably damaging	Het
Olfr938	T	C	9: 39,078,692	T18A	possibly damaging	Het
Parp8	T	C	13: 116,868,536	H663R	probably damaging	Het
Pax6	T	C	2: 105,695,360	S377P	probably benign	Het
Pi15	A	G	1: 17,602,766	D63G	probably benign	Het
Plaa	A	G	4: 94,586,258		probably benign	Het
Rbm5	C	A	9: 107,745,213	W546C	probably damaging	Het
Rnf123	C	A	9: 108,052,089	C1257F	probably damaging	Het
Rnf185	A	G	11: 3,432,551	S45P	possibly damaging	Het
Sh3bp1	T	C	15: 78,904,497	S241P	possibly damaging	Het
Slfn8	G	A	11: 83,003,197	A872V	possibly damaging	Het
Smoc1	G	A	12: 81,104,773		probably null	Het
Sox14	A	T	9: 99,875,633	W18R	probably damaging	Het
Spata31d1a	T	C	13: 59,701,155	E1053G	probably benign	Het
Syne1	T	A	10: 5,344,866	K1246*	probably null	Het
Tbrg4	A	C	11: 6,620,909	S188A	probably benign	Het
Thnsl2	A	T	6: 71,134,295	D196E	probably damaging	Het
Tmem266	C	T	9: 55,380,741	T34I	probably damaging	Het
Tmem59l	C	A	8: 70,486,098	R111L	probably benign	Het
Tpr	T	C	1: 150,430,529		probably benign	Het
Trpc6	T	C	9: 8,643,686	S491P	probably damaging	Het
Tspan10	A	T	11: 120,446,166	N254I	probably damaging	Het
Ubox5	A	T	2: 130,591,894	L511Q	probably damaging	Het
Vmn1r183	TATCCATC	TATC	7: 24,055,106		probably null	Het
Vmn2r4	C	T	3: 64,390,976	C577Y	probably damaging	Het
Zfp948	T	A	17: 21,588,307	I587N	possibly damaging	Het

Other mutations in Vmn2r86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01295:Vmn2r86	APN	10	130453026	missense	probably damaging	0.99
IGL01328:Vmn2r86	APN	10	130452496	missense	possibly damaging	0.78
IGL01377:Vmn2r86	APN	10	130452986	missense	probably damaging	0.99
IGL01548:Vmn2r86	APN	10	130446282	missense	probably benign	0.22
IGL01804:Vmn2r86	APN	10	130452989	missense	probably damaging	0.99
IGL01921:Vmn2r86	APN	10	130455741	missense	probably benign	0.00
IGL02406:Vmn2r86	APN	10	130448639	missense	possibly damaging	0.81
IGL02625:Vmn2r86	APN	10	130452912	missense	probably damaging	1.00
IGL02960:Vmn2r86	APN	10	130453767	missense	possibly damaging	0.74
IGL03104:Vmn2r86	APN	10	130446632	missense	probably damaging	1.00
R0408:Vmn2r86	UTSW	10	130446854	missense	probably damaging	1.00
R0437:Vmn2r86	UTSW	10	130446543	missense	probably damaging	1.00
R0577:Vmn2r86	UTSW	10	130452575	missense	probably benign	0.04
R0726:Vmn2r86	UTSW	10	130446396	missense	probably damaging	1.00
R0811:Vmn2r86	UTSW	10	130453628	missense	probably benign	0.00
R0812:Vmn2r86	UTSW	10	130453628	missense	probably benign	0.00
R1055:Vmn2r86	UTSW	10	130446357	missense	probably damaging	1.00
R1066:Vmn2r86	UTSW	10	130446276	missense	probably benign	0.01
R1199:Vmn2r86	UTSW	10	130448574	splice site	probably benign
R1332:Vmn2r86	UTSW	10	130446870	missense	probably damaging	1.00
R1568:Vmn2r86	UTSW	10	130453141	missense	probably benign	0.09
R1866:Vmn2r86	UTSW	10	130446386	missense	probably damaging	1.00
R1897:Vmn2r86	UTSW	10	130452445	missense	probably damaging	1.00
R2017:Vmn2r86	UTSW	10	130446713	missense	probably benign	0.39
R3162:Vmn2r86	UTSW	10	130455804	missense	probably damaging	0.99
R3162:Vmn2r86	UTSW	10	130455804	missense	probably damaging	0.99
R3858:Vmn2r86	UTSW	10	130455725	missense	probably benign
R4049:Vmn2r86	UTSW	10	130447097	missense	probably damaging	0.98
R4378:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4411:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4413:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4422:Vmn2r86	UTSW	10	130452976	missense	possibly damaging	0.87
R4738:Vmn2r86	UTSW	10	130447070	missense	probably damaging	0.99
R4872:Vmn2r86	UTSW	10	130453591	missense	probably damaging	0.98
R4880:Vmn2r86	UTSW	10	130453615	missense	probably benign	0.33
R5092:Vmn2r86	UTSW	10	130446587	missense	probably damaging	1.00
R5421:Vmn2r86	UTSW	10	130446936	missense	probably benign	0.41
R6007:Vmn2r86	UTSW	10	130453666	missense	probably damaging	1.00
R6330:Vmn2r86	UTSW	10	130446527	missense	probably benign	0.05
R6355:Vmn2r86	UTSW	10	130455894	start codon destroyed	probably damaging	0.98
R6397:Vmn2r86	UTSW	10	130446262	nonsense	probably null
R6419:Vmn2r86	UTSW	10	130446926	missense	probably damaging	1.00
R6933:Vmn2r86	UTSW	10	130446257	missense	probably damaging	1.00
R6937:Vmn2r86	UTSW	10	130448654	missense	probably damaging	1.00
R6959:Vmn2r86	UTSW	10	130446531	missense	probably damaging	1.00
R7010:Vmn2r86	UTSW	10	130455857	missense	probably benign
R7549:Vmn2r86	UTSW	10	130446828	missense	probably damaging	1.00
R8179:Vmn2r86	UTSW	10	130453084	missense	probably benign	0.00
R8257:Vmn2r86	UTSW	10	130452410	missense	possibly damaging	0.87
R8286:Vmn2r86	UTSW	10	130449986	missense	probably benign	0.03
R8479:Vmn2r86	UTSW	10	130446866	missense	probably damaging	1.00
R8805:Vmn2r86	UTSW	10	130446527	missense	probably benign	0.05
R8960:Vmn2r86	UTSW	10	130453803	missense	probably benign	0.27
R9021:Vmn2r86	UTSW	10	130447065	missense	probably damaging	1.00
R9120:Vmn2r86	UTSW	10	130453808	missense	probably benign	0.00
R9137:Vmn2r86	UTSW	10	130446540	missense	probably damaging	1.00
R9311:Vmn2r86	UTSW	10	130452571	missense	probably damaging	1.00
R9312:Vmn2r86	UTSW	10	130452537	missense	probably benign	0.02
R9433:Vmn2r86	UTSW	10	130446698	missense	possibly damaging	0.88
R9696:Vmn2r86	UTSW	10	130449833	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- GTCCAGCATTGTGAACTGGAG -3'
(R):5'- TCCATCTTAGATTCTAGCACACATG -3'

Sequencing Primer
(F):5'- CAGCATTGTGAACTGGAGAACAATG -3'
(R):5'- GATTCTAGCACACATGAAGAAAATG -3'

Posted On

2015-12-21