Mutagenetix > Incidental Mutation

Incidental Mutation 'R4785:Vcan'

367011

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, DPEAAE, heart defect, Cspg2, 5430420N07Rik

MMRRC Submission

042417-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4785 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

89655312-89742509 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 89705789 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Aspartic acid at position 351 (N351D)

Ref Sequence

ENSEMBL: ENSMUSP00000105173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000109543

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109544
AA Change: N351D

PolyPhen 2 Score 0.436 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614
AA Change: N351D

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109546
AA Change: N351D

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: N351D

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000159910

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160029

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 145 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921517D22Rik	T	C	13: 59,691,592	D13G	probably benign	Het
4930442H23Rik	C	T	10: 81,183,144		probably benign	Het
5330417C22Rik	A	T	3: 108,458,227		probably benign	Het
Adamts13	T	A	2: 26,983,042	S341T	probably damaging	Het
Adamts2	A	G	11: 50,792,722	I944V	probably benign	Het
Adcy3	A	G	12: 4,206,542	T783A	probably benign	Het
Ago3	A	T	4: 126,368,503	M418K	probably benign	Het
Alpk1	G	T	3: 127,687,592	N175K	possibly damaging	Het
Als2	A	G	1: 59,215,313	V295A	probably benign	Het
Anapc13	T	C	9: 102,629,821	I11T	probably benign	Het
Ankrd12	T	A	17: 65,982,999	N1813I	probably damaging	Het
Anks1b	A	T	10: 90,914,750	I176F	probably null	Het
Ap3d1	TTCTCTCTCTCTCTCTCT	TTCTCTCTCTCTCTCT	10: 80,712,778		probably null	Het
Arhgap32	A	G	9: 32,129,653	D67G	probably damaging	Het
Arhgap32	T	C	9: 32,260,780	C1619R	probably damaging	Het
Atp1a4	A	T	1: 172,254,110	Y158*	probably null	Het
Atp8b5	A	T	4: 43,356,980	D576V	probably damaging	Het
Atrnl1	A	G	19: 57,629,158	I122V	probably damaging	Het
B430203G13Rik	A	T	12: 17,924,519		noncoding transcript	Het
Baz1b	T	G	5: 135,217,413	L572R	possibly damaging	Het
Ccnd2	T	C	6: 127,148,798	T92A	possibly damaging	Het
Ccndbp1	A	T	2: 121,008,522	T5S	probably benign	Het
Ccr2	G	A	9: 124,106,372	V230I	probably benign	Het
Cct4	T	A	11: 23,002,866	V514E	probably damaging	Het
Cep152	A	G	2: 125,586,329		probably null	Het
Cflar	C	T	1: 58,752,567	T343M	probably benign	Het
Chrm5	A	C	2: 112,479,585	N395K	probably benign	Het
Chrna10	G	A	7: 102,113,219	P255S	possibly damaging	Het
Chrna6	T	A	8: 27,407,106	I248F	probably damaging	Het
Clip1	T	C	5: 123,579,293	D1387G	probably damaging	Het
Clstn3	T	C	6: 124,437,372		probably null	Het
Cntnap5a	C	T	1: 116,101,565	R250W	probably benign	Het
Col12a1	C	A	9: 79,678,494	V1228F	possibly damaging	Het
Cxcr5	T	C	9: 44,513,341	T340A	probably benign	Het
Cyp2a4	G	A	7: 26,312,875	R361K	probably damaging	Het
Dnah1	T	A	14: 31,263,479	K3818N	probably damaging	Het
Dnah3	T	A	7: 119,967,824	K2393M	probably benign	Het
Dpy19l1	A	T	9: 24,424,823	L529Q	probably damaging	Het
Eif4g2	C	T	7: 111,076,796	R399H	probably damaging	Het
Fam160a1	G	T	3: 85,688,570	T115K	probably damaging	Het
Fam76a	A	T	4: 132,902,117		probably null	Het
Fam76a	A	G	4: 132,916,190	Y78H	probably damaging	Het
Fbn1	A	T	2: 125,324,919	C2026S	probably damaging	Het
Fbxo4	T	C	15: 3,969,041	T312A	probably benign	Het
Fbxw26	A	G	9: 109,724,800	V257A	possibly damaging	Het
Flnb	A	T	14: 7,905,701	E1150D	probably benign	Het
Fndc3c1	A	T	X: 106,437,702	S661R	possibly damaging	Het
Gba2	A	C	4: 43,568,315	L684R	probably damaging	Het
Gbf1	G	T	19: 46,268,395	C812F	possibly damaging	Het
Glt8d2	A	T	10: 82,660,749	D158E	probably damaging	Het
Golga2	T	A	2: 32,297,156	N89K	probably damaging	Het
Got1	A	G	19: 43,502,937	I358T	possibly damaging	Het
Gpx6	C	T	13: 21,312,264	Q3*	probably null	Het
Grin1	T	A	2: 25,292,381	H956L	possibly damaging	Het
Grin2d	A	G	7: 45,856,781	V562A	probably damaging	Het
Grm6	A	G	11: 50,857,277	I405V	probably benign	Het
Hcar2	C	G	5: 123,864,450	G330A	probably benign	Het
Hcfc1	T	C	X: 73,965,946	K20E	probably damaging	Het
Iars	T	A	13: 49,724,663	Y888N	probably damaging	Het
Ifi206	A	T	1: 173,480,866	H521Q	probably benign	Het
Impg1	A	G	9: 80,398,450	S112P	probably benign	Het
Incenp	C	A	19: 9,877,690	R619M	unknown	Het
Incenp	T	A	19: 9,877,691	R619W	unknown	Het
Kat2b	A	G	17: 53,653,203	E513G	possibly damaging	Het
Kbtbd12	G	T	6: 88,618,021	L276I	probably damaging	Het
Kctd9	T	A	14: 67,734,164	D229E	probably damaging	Het
Kdm1b	C	T	13: 47,063,077	R308W	probably damaging	Het
Kif2b	G	A	11: 91,576,428	P343L	probably benign	Het
Kntc1	T	G	5: 123,816,762	M2081R	possibly damaging	Het
Lamc1	T	C	1: 153,231,740	N1231S	probably damaging	Het
Lclat1	T	A	17: 73,240,070	Y327*	probably null	Het
Lgals3bp	G	A	11: 118,393,514	T413I	probably damaging	Het
Lin54	A	G	5: 100,459,738	I250T	probably damaging	Het
Lrp1	T	C	10: 127,558,133	N2621S	probably benign	Het
Lrrtm3	T	A	10: 64,088,002	H462L	probably benign	Het
Lysmd1	A	G	3: 95,134,986	Y57C	probably damaging	Het
Mccc1	C	A	3: 35,975,873	M429I	probably damaging	Het
Metrnl	A	G	11: 121,707,924	E40G	probably benign	Het
Mios	T	A	6: 8,222,464	M466K	probably benign	Het
Mrgpra1	A	G	7: 47,335,470	S154P	probably damaging	Het
Mrpl24	T	C	3: 87,922,050		probably null	Het
Myo1f	A	G	17: 33,598,191	N736S	possibly damaging	Het
Myo1h	A	G	5: 114,360,599	I919V	possibly damaging	Het
Nampt	T	C	12: 32,848,714	L443P	possibly damaging	Het
Ndufaf2	C	G	13: 108,052,780	A145P	probably damaging	Het
Nek10	A	T	14: 14,855,714	T403S	probably benign	Het
Nme8	C	A	13: 19,657,930	V219L	probably damaging	Het
Nov	A	T	15: 54,752,207		probably null	Het
Nps	T	A	7: 135,268,788	L13Q	probably damaging	Het
Nutm1	G	A	2: 112,248,936	A878V	probably benign	Het
Ogdh	T	C	11: 6,349,875	F794L	probably damaging	Het
Olfr1305	A	T	2: 111,873,050	D268E	possibly damaging	Het
Olfr1441	A	T	19: 12,422,977	I223F	probably damaging	Het
Olfr292	T	C	7: 86,694,528	L24P	probably damaging	Het
Olfr305	C	A	7: 86,363,735	G201C	probably damaging	Het
Olfr721-ps1	A	C	14: 14,407,729	Y167S	possibly damaging	Het
Olfr998	A	G	2: 85,590,938	T133A	probably benign	Het
Pik3cg	T	C	12: 32,205,199	E263G	probably damaging	Het
Pikfyve	A	G	1: 65,267,846	T1798A	probably benign	Het
Poldip3	T	C	15: 83,131,501	Y305C	probably damaging	Het
Pqlc2	G	T	4: 139,300,001	H343Q	probably benign	Het
Prkdc	T	C	16: 15,648,976	I107T	probably benign	Het
Prmt2	A	G	10: 76,226,221	I50T	probably damaging	Het
Ptprd	T	C	4: 76,140,553	T168A	probably benign	Het
Pudp	A	G	18: 50,568,065	V199A	probably damaging	Het
Rab3c	T	C	13: 110,061,900	E198G	probably benign	Het
Rasl12	A	G	9: 65,413,448	Y156C	probably damaging	Het
Rbm12	T	C	2: 156,096,564	D596G	possibly damaging	Het
Rbm6	T	C	9: 107,787,352	E694G	probably benign	Het
Rif1	T	C	2: 52,112,747	V2071A	probably damaging	Het
Ripply2	A	T	9: 87,019,796	N125I	probably damaging	Het
Rps6kc1	A	T	1: 190,750,188	H284Q	probably benign	Het
Scara3	A	T	14: 65,953,501	M1K	probably null	Het
Sfswap	A	G	5: 129,513,083	T215A	probably damaging	Het
Shroom2	A	C	X: 152,660,907	F421V	probably benign	Het
Sipa1l3	A	G	7: 29,377,641	V902A	probably damaging	Het
Slamf8	G	T	1: 172,584,214	P238Q	probably damaging	Het
Slc44a3	T	C	3: 121,527,074	T93A	possibly damaging	Het
Slc5a7	A	T	17: 54,278,700	I363N	probably damaging	Het
Slc7a2	T	A	8: 40,911,058	M436K	probably benign	Het
Spindoc	A	G	19: 7,374,091	S223P	probably benign	Het
Stk11ip	T	C	1: 75,530,281	F669L	possibly damaging	Het
Sun3	G	A	11: 9,038,266	L19F	probably benign	Het
Sycp1	C	A	3: 102,853,489	A703S	possibly damaging	Het
Tas2r139	T	G	6: 42,141,284	F117V	probably damaging	Het
Tdrd6	T	C	17: 43,625,576	Y1527C	probably damaging	Het
Tenm4	A	C	7: 96,774,046	K683Q	probably damaging	Het
Tfeb	T	A	17: 47,788,227		probably null	Het
Tmco4	T	C	4: 138,998,039	S121P	probably damaging	Het
Tmprss11d	C	T	5: 86,306,281	V222M	probably damaging	Het
Tnks1bp1	T	C	2: 85,063,034	W440R	probably damaging	Het
Trp53bp2	A	G	1: 182,448,997	T848A	probably benign	Het
Tssk1	G	A	16: 17,895,062	R237H	probably benign	Het
Ttll4	A	T	1: 74,679,007	T6S	possibly damaging	Het
Ttn	G	T	2: 76,721,797	Y29419*	probably null	Het
Ttn	A	G	2: 76,769,603	Y19076H	probably damaging	Het
Ubr3	T	A	2: 69,959,603	V867D	probably damaging	Het
Usp29	A	G	7: 6,961,391	T78A	probably damaging	Het
Utrn	T	G	10: 12,654,745	Q2108P	probably benign	Het
Vmn1r61	A	T	7: 5,611,125	Y63*	probably null	Het
Vmn1r61	A	T	7: 5,611,127	Y63N	probably benign	Het
Vmn1r85	A	T	7: 13,084,861	W119R	probably damaging	Het
Zbtb21	A	C	16: 97,950,455	V190G	possibly damaging	Het
Zfp157	C	A	5: 138,444,789	T14N	probably damaging	Het
Zfp942	A	T	17: 21,929,419	H76Q	probably damaging	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89704702	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89692319	missense	probably benign
IGL00504:Vcan	APN	13	89691275	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89688979	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89703726	missense	probably benign
IGL00743:Vcan	APN	13	89725306	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89662052	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89679958	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89691668	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89703943	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89704169	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89688675	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89690586	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89689745	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89725388	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89689205	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89689359	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89684438	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89725565	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89684493	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89703077	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89704849	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89690657	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89688623	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89703275	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89661932	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89705006	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89661985	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89703668	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89703668	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89678073	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89691261	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89712191	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89691275	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89703546	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89703199	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89678145	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89704660	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89703772	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89712253	missense	probably damaging	1.00
R0561:Vcan	UTSW	13	89731464	missense	possibly damaging	0.86
R0636:Vcan	UTSW	13	89704706	missense	probably damaging	0.99
R0636:Vcan	UTSW	13	89712267	missense	probably damaging	1.00
R0680:Vcan	UTSW	13	89679822	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89704953	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89685077	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89692410	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89705663	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89704303	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89679794	splice site	probably null
R1219:Vcan	UTSW	13	89679904	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89657556	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89693055	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89693055	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89688484	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89688484	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89692956	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89689661	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89705663	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89703667	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89661946	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89703547	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89688483	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89721775	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89704735	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89691681	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89705212	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89705392	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89690871	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89693015	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89705534	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89702926	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89703424	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89692742	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89689038	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89690985	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89693303	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89693303	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89693303	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89689737	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89703529	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89703410	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89689449	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89704675	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89704237	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89703301	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89692543	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89692543	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89692543	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89679887	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89685158	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89725547	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89725486	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89704028	missense	probably damaging	0.97
R4666:Vcan	UTSW	13	89679934	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89704739	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89688842	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89703977	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89657472	intron	probably benign
R5124:Vcan	UTSW	13	89725517	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89690240	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89690872	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89692532	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89691600	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89690286	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89691810	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89703112	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89688671	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89691503	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89691135	frame shift	probably null
R5687:Vcan	UTSW	13	89678134	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89679950	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89703952	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89692691	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89657536	nonsense	probably null
R6135:Vcan	UTSW	13	89689926	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89691220	nonsense	probably null
R6280:Vcan	UTSW	13	89725373	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89691597	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89704832	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89690687	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89704731	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89705182	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89705125	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89712381	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89689431	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89678133	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89679956	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89693407	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89690618	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89690241	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89705686	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89689110	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89725591	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89688936	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89705270	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89721686	missense	probably damaging	1.00
R7250:Vcan	UTSW	13	89731457	critical splice donor site	probably null
R7262:Vcan	UTSW	13	89705161	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89692733	nonsense	probably null
R7299:Vcan	UTSW	13	89705266	missense	probably benign
R7301:Vcan	UTSW	13	89705266	missense	probably benign
R7425:Vcan	UTSW	13	89689832	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89704118	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89692458	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89692223	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89685114	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89685114	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89691789	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89704619	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89689323	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89692937	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89688654	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89688654	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89691858	missense	probably benign
R7998:Vcan	UTSW	13	89704327	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89704360	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89657290	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89657658	missense	probably damaging	1.00
R8103:Vcan	UTSW	13	89703320	nonsense	probably null
R8124:Vcan	UTSW	13	89704254	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89704987	nonsense	probably null
R8166:Vcan	UTSW	13	89692736	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89704970	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89704335	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89688743	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89691098	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89692320	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89692111	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89704907	missense	probably benign
R9049:Vcan	UTSW	13	89678105	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89691027	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89704525	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89679931	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89690496	nonsense	probably null
R9259:Vcan	UTSW	13	89690870	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89689333	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89703412	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89693009	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89691540	missense
R9579:Vcan	UTSW	13	89689594	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89705372	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89692962	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89691741	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89691128	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89689811	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89692493	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89705749	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89692571	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89703524	missense	probably benign	0.00
Z1177:Vcan	UTSW	13	89703788	nonsense	probably null
Z1177:Vcan	UTSW	13	89704073	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGACTATATTTCCTGCAGGAGG -3'
(R):5'- AACAAGTCAAGTTCTCCTTCTCAC -3'

Sequencing Primer
(F):5'- TTCCTGCAGGAGGGAAGTG -3'
(R):5'- TGTAGCTCATTACAGAGAACCATC -3'

Posted On

2015-12-29