Mutagenetix > Incidental Mutation

Incidental Mutation 'R4788:Cyp3a25'

367247

Institutional Source

Beutler Lab

Gene Symbol

Cyp3a25

Ensembl Gene

ENSMUSG00000029630

Gene Name

cytochrome P450, family 3, subfamily a, polypeptide 25

Synonyms

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.129) question?

Stock #

R4788 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

145977194-146009618 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 145985082 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 347 (Y347*)

Ref Sequence

ENSEMBL: ENSMUSP00000065585 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068317] [ENSMUST00000138870] [ENSMUST00000145062]

AlphaFold

O09158

Predicted Effect

probably null
Transcript: ENSMUST00000068317
AA Change: Y347*

SMART Domains

Protein: ENSMUSP00000065585
Gene: ENSMUSG00000029630
AA Change: Y347*

Domain	Start	End	E-Value	Type
Pfam:p450	38	493	9.4e-129	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000138870

SMART Domains

Protein: ENSMUSP00000116077
Gene: ENSMUSG00000029630

Domain	Start	End	E-Value	Type
Pfam:p450	38	126	2e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000145062

SMART Domains

Protein: ENSMUSP00000123615
Gene: ENSMUSG00000029630

Domain	Start	End	E-Value	Type
Pfam:p450	38	148	3.9e-21	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153808

Coding Region Coverage

1x: 98.8%
3x: 98.4%
10x: 96.9%
20x: 94.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010300C02Rik	A	T	1: 37,631,475	I128K	probably damaging	Het
Abca4	G	A	3: 122,166,712	D815N	probably damaging	Het
Abcc9	A	T	6: 142,620,730	C1135*	probably null	Het
Ap3b1	G	A	13: 94,565,641	M1067I	unknown	Het
Arhgap15	T	C	2: 43,748,890	M1T	probably null	Het
Boc	T	C	16: 44,500,433	D288G	probably damaging	Het
Brsk1	G	T	7: 4,698,955		probably null	Het
Cabp1	T	C	5: 115,175,471	N226S	probably benign	Het
Capn13	A	T	17: 73,337,432	Y367*	probably null	Het
Ccdc191	A	G	16: 43,956,822	E632G	probably damaging	Het
Cit	T	C	5: 115,933,506	S591P	probably damaging	Het
Ckmt1	T	C	2: 121,359,946	V118A	possibly damaging	Het
Cndp2	T	C	18: 84,675,164	N157S	probably damaging	Het
Col6a3	A	G	1: 90,772,950		probably null	Het
Coq2	A	T	5: 100,657,909	V287E	probably damaging	Het
Cpa6	T	C	1: 10,408,277	K278R	possibly damaging	Het
Cybb	C	G	X: 9,450,750	D246H	probably benign	Het
Dcun1d4	G	T	5: 73,534,628	W160L	probably damaging	Het
Dennd4c	A	G	4: 86,819,963	T994A	probably benign	Het
Dhx57	T	C	17: 80,275,331	T229A	probably benign	Het
Dnah6	T	A	6: 73,129,530	R1741S	probably damaging	Het
Dock1	T	A	7: 135,145,484	V1508D	probably damaging	Het
Donson	T	C	16: 91,687,833	T117A	possibly damaging	Het
Dtnb	A	G	12: 3,772,699	D533G	probably damaging	Het
Fcrl5	A	G	3: 87,457,188	N470S	probably damaging	Het
Focad	T	A	4: 88,357,469	V1105E	unknown	Het
Fry	T	C	5: 150,399,636	V1084A	probably benign	Het
Gabra1	G	T	11: 42,147,153	R213S	probably damaging	Het
Gbp2b	A	C	3: 142,611,410	K509T	probably benign	Het
Gprc6a	T	C	10: 51,615,008	T811A	probably benign	Het
Hmgcs2	A	G	3: 98,291,084	D101G	probably damaging	Het
Ifna11	G	A	4: 88,820,008	W17*	probably null	Het
Ighv1-62-3	T	A	12: 115,461,052	M100L	probably benign	Het
Lgmn	T	A	12: 102,402,677	Y181F	probably benign	Het
Map4k4	T	C	1: 40,003,916	S644P	probably benign	Het
Med9	T	A	11: 59,948,440	N58K	probably benign	Het
Nav1	C	T	1: 135,469,723	A903T	probably benign	Het
Nop53	T	C	7: 15,942,315	E153G	possibly damaging	Het
Nop56	G	T	2: 130,278,900	V190L	probably benign	Het
Nyap2	A	T	1: 81,269,397	M687L	probably benign	Het
Olfr1254	G	A	2: 89,789,136	S72F	probably damaging	Het
Olfr146	T	C	9: 39,018,921	T207A	probably benign	Het
Olfr943	A	G	9: 39,184,612	T145A	probably benign	Het
Osbp2	T	C	11: 3,863,320	K183R	probably benign	Het
Pappa2	A	G	1: 158,783,917	V1492A	possibly damaging	Het
Pax1	A	G	2: 147,366,204	Q244R	possibly damaging	Het
Pcdh9	C	A	14: 93,887,415	V317F	probably damaging	Het
Pkhd1l1	A	T	15: 44,498,021	Q489L	probably damaging	Het
Poln	A	T	5: 34,129,331	S164R	probably benign	Het
Ptf1a	T	C	2: 19,445,951	S31P	probably benign	Het
Rasal3	A	T	17: 32,399,338	D164E	probably benign	Het
Rnf170	A	G	8: 26,140,863	E168G	probably damaging	Het
Rubcn	A	G	16: 32,836,408		probably null	Het
Sec16b	A	T	1: 157,561,524	T830S	possibly damaging	Het
Sel1l3	A	G	5: 53,131,833	V882A	probably benign	Het
Sfpq	A	G	4: 127,025,998	E512G	probably damaging	Het
Sh3pxd2a	A	G	19: 47,314,079	L187P	probably damaging	Het
Skint6	C	T	4: 113,238,336	G42D	possibly damaging	Het
Slc7a2	A	G	8: 40,913,986	I526V	probably benign	Het
Spata48	T	C	11: 11,488,652		probably null	Het
Ssh2	A	T	11: 77,429,798	N328Y	probably damaging	Het
Taok2	G	A	7: 126,868,132	S167L	possibly damaging	Het
Tcrg-V5	A	G	13: 19,192,554	K57R	probably benign	Het
Tom1l2	A	G	11: 60,249,018	L275P	probably damaging	Het
Tyrp1	C	T	4: 80,844,943	R356*	probably null	Het
Zan	A	G	5: 137,442,113	L1953P	unknown	Het
Zfhx3	T	C	8: 108,794,210	S655P	probably damaging	Het
Zmym1	T	C	4: 127,054,297	T94A	probably benign	Het

Other mutations in Cyp3a25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Cyp3a25	APN	5	146001463	nonsense	probably null
IGL00430:Cyp3a25	APN	5	145993360	missense	probably damaging	1.00
IGL00803:Cyp3a25	APN	5	146001443	splice site	probably benign
IGL00928:Cyp3a25	APN	5	145986954	missense	possibly damaging	0.94
IGL01557:Cyp3a25	APN	5	145984901	missense	probably damaging	1.00
IGL01997:Cyp3a25	APN	5	145994956	missense	possibly damaging	0.92
IGL02140:Cyp3a25	APN	5	146009463	splice site	probably benign
IGL02267:Cyp3a25	APN	5	145998552	missense	possibly damaging	0.48
IGL02272:Cyp3a25	APN	5	145993265	intron	probably benign
IGL02327:Cyp3a25	APN	5	145986921	missense	possibly damaging	0.50
IGL02411:Cyp3a25	APN	5	146001447	critical splice donor site	probably benign
IGL02504:Cyp3a25	APN	5	145993331	missense	probably benign	0.03
IGL02653:Cyp3a25	APN	5	146003110	missense	possibly damaging	0.95
R0378:Cyp3a25	UTSW	5	145986842	missense	probably damaging	1.00
R0403:Cyp3a25	UTSW	5	145998513	missense	probably damaging	1.00
R0685:Cyp3a25	UTSW	5	145998546	missense	probably damaging	1.00
R0725:Cyp3a25	UTSW	5	145994936	missense	probably damaging	1.00
R0798:Cyp3a25	UTSW	5	145991533	missense	probably damaging	0.98
R1061:Cyp3a25	UTSW	5	145986833	missense	probably benign
R1519:Cyp3a25	UTSW	5	146001447	critical splice donor site	probably null
R1628:Cyp3a25	UTSW	5	146001463	nonsense	probably null
R1822:Cyp3a25	UTSW	5	145984953	missense	probably damaging	1.00
R1824:Cyp3a25	UTSW	5	145984953	missense	probably damaging	1.00
R1864:Cyp3a25	UTSW	5	145994929	missense	probably damaging	0.98
R2062:Cyp3a25	UTSW	5	145986969	splice site	probably benign
R2401:Cyp3a25	UTSW	5	145986968	critical splice acceptor site	probably null
R2516:Cyp3a25	UTSW	5	146003027	splice site	probably null
R3080:Cyp3a25	UTSW	5	145998531	missense	probably benign	0.33
R3236:Cyp3a25	UTSW	5	146003128	splice site	probably benign
R3694:Cyp3a25	UTSW	5	145989976	splice site	probably null
R3730:Cyp3a25	UTSW	5	146003081	missense	probably damaging	1.00
R4112:Cyp3a25	UTSW	5	146003031	missense	probably benign	0.18
R4258:Cyp3a25	UTSW	5	145991438	missense	probably damaging	1.00
R4651:Cyp3a25	UTSW	5	145994891	missense	probably benign	0.01
R4899:Cyp3a25	UTSW	5	145977671	missense	possibly damaging	0.59
R4926:Cyp3a25	UTSW	5	145991456	missense	probably damaging	1.00
R4952:Cyp3a25	UTSW	5	145991524	missense	probably benign	0.01
R5270:Cyp3a25	UTSW	5	145981502	missense	probably benign	0.36
R5595:Cyp3a25	UTSW	5	145994863	critical splice donor site	probably null
R5659:Cyp3a25	UTSW	5	145991546	missense	possibly damaging	0.69
R5787:Cyp3a25	UTSW	5	145998503	missense	probably benign	0.14
R6307:Cyp3a25	UTSW	5	145994956	missense	possibly damaging	0.92
R6380:Cyp3a25	UTSW	5	145998547	missense	probably damaging	0.99
R7055:Cyp3a25	UTSW	5	145992991	missense	probably benign	0.00
R7140:Cyp3a25	UTSW	5	146003045	missense	probably benign
R7189:Cyp3a25	UTSW	5	146003060	missense	probably benign	0.37
R7201:Cyp3a25	UTSW	5	145991447	missense	probably benign	0.22
R7201:Cyp3a25	UTSW	5	146003058	missense	probably benign	0.00
R7332:Cyp3a25	UTSW	5	145993007	missense	probably damaging	1.00
R7404:Cyp3a25	UTSW	5	145986825	missense	probably damaging	1.00
R7548:Cyp3a25	UTSW	5	145986925	missense	probably damaging	0.98
R7607:Cyp3a25	UTSW	5	145984981	missense	possibly damaging	0.87
R8022:Cyp3a25	UTSW	5	145977668	missense	probably benign	0.33
R8266:Cyp3a25	UTSW	5	145992986	missense	probably damaging	1.00
R8894:Cyp3a25	UTSW	5	145994860	splice site	probably benign
R9249:Cyp3a25	UTSW	5	145991546	missense	possibly damaging	0.69
R9588:Cyp3a25	UTSW	5	145984889	missense	probably benign
R9691:Cyp3a25	UTSW	5	145994922	missense	probably benign	0.41
R9694:Cyp3a25	UTSW	5	145986875	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGAATTCCTGAGGCTCTGGC -3'
(R):5'- GACCACTCTTACCAGGATTCTC -3'

Sequencing Primer
(F):5'- CCAGTACTCAGGATTTCGGTGAAG -3'
(R):5'- ATCTGGCCAGGGGAGTATC -3'

Posted On

2015-12-29