Mutagenetix > Incidental Mutations

Incidental Mutation 'R4778:Vmn2r65'

368083

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r65

Ensembl Gene

ENSMUSG00000066372

Gene Name

vomeronasal 2, receptor 65

Synonyms

ENSMUSG00000070600

MMRRC Submission

042414-MU

Accession Numbers

Genbank: NM_001105180; MGI: 3642776

Is this an essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R4778 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

84940169-84964009 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 84943593 bp

Zygosity

Heterozygous

Amino Acid Change

Lysine to Glutamic Acid at position 469 (K469E)

Ref Sequence

ENSEMBL: ENSMUSP00000036551 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044583]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000044583
AA Change: K469E

PolyPhen 2 Score 0.473 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000036551
Gene: ENSMUSG00000066372
AA Change: K469E

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	73	435	5.6e-41	PFAM
Pfam:NCD3G	501	553	1.3e-21	PFAM
Pfam:7tm_3	584	821	2.3e-53	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

98% (54/55)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610037L13Rik	T	C	4: 107,891,998	V64A	probably damaging	Het
4932438A13Rik	T	A	3: 36,937,065	M897K	possibly damaging	Het
Abcc10	T	C	17: 46,304,416	N1349S	probably damaging	Het
AF529169	T	A	9: 89,603,102	I81F	probably damaging	Het
Ahnak	T	C	19: 9,011,975	V3541A	possibly damaging	Het
Arhgap33	T	A	7: 30,532,093	T156S	probably benign	Het
Card11	G	T	5: 140,883,782		probably null	Het
Cdh3	T	A	8: 106,543,826	I445N	probably damaging	Het
Csrp3	T	G	7: 48,832,563	K169N	probably damaging	Het
Cyp2c69	T	C	19: 39,877,594	N185S	probably benign	Het
Dpysl3	C	T	18: 43,354,802	V159I	probably benign	Het
Erc1	A	T	6: 119,797,337		probably null	Het
Fat1	T	C	8: 45,038,326	V3808A	probably benign	Het
Fbxw19	T	C	9: 109,494,646	D87G	probably damaging	Het
Gm1123	T	C	9: 99,018,507	I99V	probably benign	Het
Gm11677	C	T	11: 111,724,711		noncoding transcript	Het
Gm5045	T	A	15: 59,211,403		noncoding transcript	Het
Hand1	T	C	11: 57,831,623	D55G	possibly damaging	Het
Lrguk	T	A	6: 34,056,080	I227K	probably damaging	Het
Mdn1	C	T	4: 32,683,583	R726*	probably null	Het
Myo16	T	A	8: 10,569,694	V1415E	probably damaging	Het
Myof	T	C	19: 37,949,563	D901G	probably damaging	Het
Naip1	A	G	13: 100,426,648	Y670H	probably damaging	Het
Nmd3	T	A	3: 69,731,591	Y171*	probably null	Het
Notch4	C	T	17: 34,582,511	A1111V	possibly damaging	Het
Nphp4	T	A	4: 152,556,291	D1038E	probably benign	Het
Olfr484	A	T	7: 108,124,480	I261N	possibly damaging	Het
Olfr694	A	C	7: 106,689,667	S21R	probably damaging	Het
Osbpl10	C	T	9: 115,109,530	S86L	probably damaging	Het
Pcsk6	G	A	7: 65,959,145	G252R	probably damaging	Het
Pole	T	G	5: 110,330,832	H15Q	probably benign	Het
Pstpip1	A	G	9: 56,128,620	D383G	possibly damaging	Het
Ptprq	T	C	10: 107,591,022	T1551A	probably benign	Het
Rasgrf2	A	G	13: 91,983,661	F626L	probably damaging	Het
Retreg1	C	A	15: 25,971,785	N394K	possibly damaging	Het
Rpp14	T	A	14: 8,090,203	D42E	probably benign	Het
Rrp8	T	A	7: 105,737,274		probably benign	Het
Rufy4	T	C	1: 74,147,663	C537R	probably damaging	Het
Snx1	T	C	9: 66,101,416		probably benign	Het
Stau1	A	T	2: 166,963,522	N51K	probably benign	Het
Tdrd5	T	A	1: 156,255,587	D960V	probably damaging	Het
Tex10	T	C	4: 48,436,468	D750G	probably damaging	Het
Tmem43	T	C	6: 91,482,255	V236A	probably damaging	Het
Tmem89	T	C	9: 108,915,375	V112A	probably damaging	Het
Traf7	C	G	17: 24,510,438		probably benign	Het
Vmn1r41	A	C	6: 89,747,275	K266T	probably damaging	Het
Zfp831	A	G	2: 174,646,807	T1092A	possibly damaging	Het
Zfp981	T	A	4: 146,537,655	S346T	probably benign	Het

Other mutations in Vmn2r65

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00942:Vmn2r65	APN	7	84943553	missense	probably damaging	0.99
IGL01104:Vmn2r65	APN	7	84940788	missense	possibly damaging	0.93
IGL01154:Vmn2r65	APN	7	84943521	missense	probably benign	0.00
IGL01453:Vmn2r65	APN	7	84940500	missense	probably damaging	1.00
IGL01675:Vmn2r65	APN	7	84947379	missense	probably benign	0.00
IGL01748:Vmn2r65	APN	7	84940299	missense	probably damaging	1.00
IGL01997:Vmn2r65	APN	7	84940770	missense	probably damaging	1.00
IGL02527:Vmn2r65	APN	7	84946516	missense	possibly damaging	0.82
IGL02617:Vmn2r65	APN	7	84940341	missense	probably damaging	1.00
IGL02676:Vmn2r65	APN	7	84940173	missense	possibly damaging	0.86
IGL03084:Vmn2r65	APN	7	84943146	missense	probably damaging	1.00
A4554:Vmn2r65	UTSW	7	84946583	missense	probably damaging	0.96
PIT4651001:Vmn2r65	UTSW	7	84946253	missense	probably benign	0.12
R0322:Vmn2r65	UTSW	7	84946548	missense	probably benign	0.01
R0453:Vmn2r65	UTSW	7	84946234	missense	probably benign	0.03
R0862:Vmn2r65	UTSW	7	84943645	missense	probably benign	0.00
R1392:Vmn2r65	UTSW	7	84947416	missense	probably benign	0.14
R1392:Vmn2r65	UTSW	7	84947416	missense	probably benign	0.14
R1508:Vmn2r65	UTSW	7	84940678	missense	probably benign	0.00
R1687:Vmn2r65	UTSW	7	84940818	missense	probably benign	0.02
R1876:Vmn2r65	UTSW	7	84946297	missense	probably damaging	1.00
R2136:Vmn2r65	UTSW	7	84943573	missense	probably damaging	0.96
R2259:Vmn2r65	UTSW	7	84940911	missense	possibly damaging	0.86
R2338:Vmn2r65	UTSW	7	84940843	missense	possibly damaging	0.86
R2880:Vmn2r65	UTSW	7	84963886	missense	probably damaging	1.00
R3410:Vmn2r65	UTSW	7	84946688	missense	probably benign	0.00
R3411:Vmn2r65	UTSW	7	84946688	missense	probably benign	0.00
R3770:Vmn2r65	UTSW	7	84940415	missense	probably damaging	1.00
R3800:Vmn2r65	UTSW	7	84940530	missense	possibly damaging	0.93
R3850:Vmn2r65	UTSW	7	84946651	missense	probably benign	0.00
R4105:Vmn2r65	UTSW	7	84946483	missense	probably benign	0.03
R4568:Vmn2r65	UTSW	7	84947469	missense	probably damaging	1.00
R4677:Vmn2r65	UTSW	7	84963874	missense	possibly damaging	0.55
R4768:Vmn2r65	UTSW	7	84947394	missense	probably damaging	1.00
R5057:Vmn2r65	UTSW	7	84940611	missense	probably damaging	1.00
R5279:Vmn2r65	UTSW	7	84940641	missense	probably damaging	1.00
R5280:Vmn2r65	UTSW	7	84946334	missense	probably damaging	0.99
R5394:Vmn2r65	UTSW	7	84946654	missense	probably benign	0.04
R5487:Vmn2r65	UTSW	7	84946321	missense	possibly damaging	0.76
R5580:Vmn2r65	UTSW	7	84947594	missense	probably damaging	0.99
R5638:Vmn2r65	UTSW	7	84940839	missense	probably damaging	1.00
R5673:Vmn2r65	UTSW	7	84947407	missense	probably benign	0.23
R5688:Vmn2r65	UTSW	7	84940692	missense	probably benign	0.00
R5935:Vmn2r65	UTSW	7	84943661	missense	probably benign	0.00
R6354:Vmn2r65	UTSW	7	84940366	missense	probably benign	0.35
R6372:Vmn2r65	UTSW	7	84940653	missense	probably damaging	1.00
R6924:Vmn2r65	UTSW	7	84963990	missense	probably benign	0.20
R7021:Vmn2r65	UTSW	7	84947379	missense	probably benign	0.00
R7195:Vmn2r65	UTSW	7	84943139	critical splice donor site	probably null
R7422:Vmn2r65	UTSW	7	84946361	missense	probably damaging	0.99
R7654:Vmn2r65	UTSW	7	84941053	missense	probably damaging	1.00
R7686:Vmn2r65	UTSW	7	84940536	missense	probably damaging	0.99
R7691:Vmn2r65	UTSW	7	84943643	missense	probably benign	0.30
R7798:Vmn2r65	UTSW	7	84946322	missense	probably benign	0.00
R7798:Vmn2r65	UTSW	7	84946984	missense	probably damaging	1.00
X0067:Vmn2r65	UTSW	7	84940905	missense	probably benign	0.04
Z1088:Vmn2r65	UTSW	7	84943265	critical splice acceptor site	probably null
Z1177:Vmn2r65	UTSW	7	84941026	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- TAGAAAGAGTTTGACCCACTTCAC -3'
(R):5'- TATCTACCCTAATGTGCATGCATAG -3'

Sequencing Primer
(F):5'- TGCGATTTGCAGTCTTATT -3'
(R):5'- CCTTTCCTGGAGAACAACA -3'

Posted On

2015-12-29