Mutagenetix > Incidental Mutation

Incidental Mutation 'R4779:Vmn2r73'

368150

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r73

Ensembl Gene

ENSMUSG00000070458

Gene Name

vomeronasal 2, receptor 73

Synonyms

EG620928

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.172) question?

Stock #

R4779 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85857547-85875938 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 85871715 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Stop codon at position 348 (W348*)

Ref Sequence

ENSEMBL: ENSMUSP00000076687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077478]

AlphaFold

D3Z7M3

Predicted Effect

probably null
Transcript: ENSMUST00000077478
AA Change: W348*

SMART Domains

Protein: ENSMUSP00000076687
Gene: ENSMUSG00000070458
AA Change: W348*

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.4e-30	PFAM
Pfam:NCD3G	508	560	5.9e-21	PFAM
Pfam:7tm_3	590	827	4.4e-51	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.8%

Validation Efficiency

98% (95/97)

Allele List at MGI

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410089E03Rik	T	C	15: 8,218,838	S1624P	probably benign	Het
4930562C15Rik	T	A	16: 4,849,749	S335T	unknown	Het
4933421I07Rik	T	A	7: 42,448,031	H13L	possibly damaging	Het
6030468B19Rik	A	G	11: 117,806,008	T185A	probably benign	Het
Abcc1	T	A	16: 14,410,771	V294E	probably benign	Het
Abhd16b	A	G	2: 181,493,460	T52A	possibly damaging	Het
Actl7a	A	T	4: 56,743,632	N53I	probably benign	Het
Ankmy1	T	C	1: 92,886,723	E354G	probably benign	Het
Arap1	T	C	7: 101,404,367	F1301S	probably damaging	Het
Arfgef1	A	T	1: 10,153,733	W1447R	probably damaging	Het
Atoh7	ATGGCGCT	AT	10: 63,100,408		probably benign	Het
BC067074	A	T	13: 113,368,336	N2000Y	possibly damaging	Het
Ccl24	T	A	5: 135,572,957	T6S	possibly damaging	Het
Cep152	G	T	2: 125,568,892	P1292Q	possibly damaging	Het
Cfap46	T	C	7: 139,659,815		probably benign	Het
Chd6	A	T	2: 160,949,557	S2627T	probably damaging	Het
Chd8	A	T	14: 52,231,506	S552T	probably damaging	Het
Ciita	C	A	16: 10,511,366	L502M	probably damaging	Het
Clic1	T	C	17: 35,052,487	F31S	probably damaging	Het
Cntnap1	T	A	11: 101,178,072	I147N	possibly damaging	Het
Crispld1	T	A	1: 17,749,607	D276E	probably benign	Het
Cspg4	A	T	9: 56,885,808	N276Y	probably damaging	Het
Cwf19l2	A	G	9: 3,410,035	M55V	possibly damaging	Het
Cyp2c69	T	C	19: 39,877,594	N185S	probably benign	Het
Dopey2	T	C	16: 93,757,081	I301T	probably damaging	Het
Epg5	T	C	18: 77,991,365	V1443A	probably benign	Het
Ephb4	T	C	5: 137,365,702	V612A	probably benign	Het
Fcgbp	T	A	7: 28,094,937	C1189S	probably damaging	Het
Fgfr2	T	G	7: 130,185,193		probably benign	Het
Fmn2	A	G	1: 174,609,895	E1144G	probably damaging	Het
Fmo3	T	C	1: 162,968,838	Y55C	probably damaging	Het
Frmpd1	A	G	4: 45,229,865	T11A	probably damaging	Het
Ghdc	G	T	11: 100,770,103	Q79K	possibly damaging	Het
Gm11677	C	T	11: 111,724,711		noncoding transcript	Het
Gm5591	T	C	7: 38,522,256	T130A	probably damaging	Het
Heg1	T	G	16: 33,719,772	D367E	probably benign	Het
Igkv13-84	C	T	6: 68,939,910	P64S	probably damaging	Het
Il10rb	T	A	16: 91,414,657	S128T	possibly damaging	Het
Il15ra	T	A	2: 11,718,306	I47N	probably damaging	Het
Il33	C	A	19: 29,958,911	S207*	probably null	Het
Itgb7	A	G	15: 102,224,413	Y155H	possibly damaging	Het
Kdm5a	A	G	6: 120,369,099		probably benign	Het
Kif11	G	A	19: 37,417,949	V987I	probably benign	Het
Krtap19-2	G	A	16: 88,873,874		probably benign	Het
Krtap24-1	T	A	16: 88,611,529	R236S	probably damaging	Het
L3mbtl2	A	G	15: 81,682,612	I406V	probably benign	Het
Lrp2	A	T	2: 69,459,715	H3593Q	possibly damaging	Het
Mavs	G	T	2: 131,240,365	W56C	probably damaging	Het
Mn1	C	T	5: 111,419,660	P499S	probably damaging	Het
Ntn5	T	A	7: 45,691,471	C178S	probably damaging	Het
Nufip2	A	G	11: 77,686,328	H34R	unknown	Het
Olfr470	T	A	7: 107,845,548	M62L	possibly damaging	Het
Olfr523	T	A	7: 140,176,450	L110Q	probably damaging	Het
Osbpl10	C	T	9: 115,109,530	S86L	probably damaging	Het
Pitpna	T	A	11: 75,620,327	M242K	possibly damaging	Het
Pnpla6	T	C	8: 3,522,838	V345A	probably benign	Het
Polk	A	G	13: 96,496,491		probably null	Het
Polr3k	A	G	2: 181,864,547	Y30C	probably damaging	Het
Pramel6	A	G	2: 87,509,597	H235R	probably benign	Het
Ptpra	G	A	2: 130,537,617	V364I	probably damaging	Het
Recql	A	G	6: 142,363,700		probably benign	Het
Ring1	T	C	17: 34,022,289		probably benign	Het
Rufy4	T	C	1: 74,147,663	C537R	probably damaging	Het
Samsn1	C	A	16: 75,947,289		noncoding transcript	Het
Scara5	A	G	14: 65,730,749	H157R	probably benign	Het
Sdc3	C	T	4: 130,819,065	P245L	probably damaging	Het
Slc18b1	T	A	10: 23,820,869	M318K	possibly damaging	Het
Slc26a10	C	T	10: 127,173,355	A638T	possibly damaging	Het
Slc35f2	T	A	9: 53,809,729	W259R	possibly damaging	Het
Spata13	G	A	14: 60,753,907	W366*	probably null	Het
Sptlc3	A	G	2: 139,589,589	T344A	probably benign	Het
Tap1	T	C	17: 34,193,891	V560A	probably damaging	Het
Tbc1d1	T	C	5: 64,278,046		probably null	Het
Tlr11	A	G	14: 50,361,250	Y231C	possibly damaging	Het
Tmem245	A	T	4: 56,936,468	S230T	possibly damaging	Het
Tnnt3	C	T	7: 142,514,283		probably benign	Het
Traf7	C	G	17: 24,510,438		probably benign	Het
Tshz2	A	G	2: 169,962,681		probably benign	Het
Tshz3	T	C	7: 36,768,972	S129P	probably damaging	Het
Ttc6	A	T	12: 57,729,451	N1727I	probably damaging	Het
Ttc7b	A	G	12: 100,403,362	S383P	probably damaging	Het
Tulp3	T	A	6: 128,323,120	I448F	probably damaging	Het
Ube3b	T	C	5: 114,404,717		probably null	Het
Vav1	G	A	17: 57,296,552	V84I	probably damaging	Het
Vav3	A	T	3: 109,508,794	K243I	possibly damaging	Het
Vmn1r25	T	A	6: 57,979,026	I93F	probably damaging	Het
Zdbf2	A	G	1: 63,303,238	R259G	possibly damaging	Het

Other mutations in Vmn2r73

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r73	APN	7	85857587	missense
IGL01151:Vmn2r73	APN	7	85857878	missense	probably damaging	0.99
IGL01408:Vmn2r73	APN	7	85872247	missense	probably benign	0.45
IGL01505:Vmn2r73	APN	7	85858059	nonsense	probably null
IGL01731:Vmn2r73	APN	7	85857549	makesense	probably null
IGL01818:Vmn2r73	APN	7	85869901	splice site	probably benign
IGL02236:Vmn2r73	APN	7	85872694	missense	probably benign	0.00
IGL02265:Vmn2r73	APN	7	85871639	missense	probably benign	0.02
IGL02266:Vmn2r73	APN	7	85875799	missense	possibly damaging	0.52
IGL02276:Vmn2r73	APN	7	85869772	critical splice donor site	probably null
IGL02284:Vmn2r73	APN	7	85857915	missense	possibly damaging	0.92
IGL02380:Vmn2r73	APN	7	85858175	missense	probably benign	0.00
IGL02466:Vmn2r73	APN	7	85872876	missense	probably damaging	1.00
IGL03172:Vmn2r73	APN	7	85858287	missense	probably benign	0.01
IGL03179:Vmn2r73	APN	7	85870260	missense	probably benign	0.04
G1Funyon:Vmn2r73	UTSW	7	85858302	missense	probably benign	0.00
R0077:Vmn2r73	UTSW	7	85875867	missense	probably benign	0.00
R0311:Vmn2r73	UTSW	7	85871789	missense	probably benign
R0413:Vmn2r73	UTSW	7	85871879	missense	possibly damaging	0.68
R0494:Vmn2r73	UTSW	7	85872932	missense	probably benign
R1523:Vmn2r73	UTSW	7	85870278	missense	probably benign	0.02
R1618:Vmn2r73	UTSW	7	85875912	nonsense	probably null
R1667:Vmn2r73	UTSW	7	85857681	missense	probably benign	0.00
R1728:Vmn2r73	UTSW	7	85857878	missense	probably damaging	1.00
R1729:Vmn2r73	UTSW	7	85857878	missense	probably damaging	1.00
R1747:Vmn2r73	UTSW	7	85858167	missense	probably damaging	1.00
R1784:Vmn2r73	UTSW	7	85857878	missense	probably damaging	1.00
R2152:Vmn2r73	UTSW	7	85857728	missense	probably benign	0.03
R2395:Vmn2r73	UTSW	7	85857767	missense	probably damaging	1.00
R2415:Vmn2r73	UTSW	7	85872223	missense	probably damaging	1.00
R2926:Vmn2r73	UTSW	7	85871663	missense	probably benign	0.17
R3719:Vmn2r73	UTSW	7	85870374	missense	probably damaging	1.00
R3766:Vmn2r73	UTSW	7	85871990	missense	probably benign
R3838:Vmn2r73	UTSW	7	85858050	missense	probably benign	0.00
R3890:Vmn2r73	UTSW	7	85857936	missense	probably benign
R4030:Vmn2r73	UTSW	7	85871836	missense	possibly damaging	0.92
R4247:Vmn2r73	UTSW	7	85872675	missense	probably damaging	0.99
R4409:Vmn2r73	UTSW	7	85871560	missense	probably damaging	0.97
R4427:Vmn2r73	UTSW	7	85857773	missense	probably damaging	1.00
R4430:Vmn2r73	UTSW	7	85870241	missense	probably benign
R4552:Vmn2r73	UTSW	7	85875847	missense	probably benign	0.12
R4906:Vmn2r73	UTSW	7	85857621	missense	probably damaging	0.99
R4942:Vmn2r73	UTSW	7	85870374	missense	probably damaging	1.00
R5280:Vmn2r73	UTSW	7	85857947	missense	probably damaging	0.98
R5344:Vmn2r73	UTSW	7	85875838	missense	probably benign	0.00
R5478:Vmn2r73	UTSW	7	85869788	missense	probably damaging	1.00
R5527:Vmn2r73	UTSW	7	85858367	missense	possibly damaging	0.65
R5691:Vmn2r73	UTSW	7	85858091	missense	probably damaging	1.00
R5826:Vmn2r73	UTSW	7	85875748	missense	possibly damaging	0.77
R6018:Vmn2r73	UTSW	7	85872667	missense	possibly damaging	0.48
R6082:Vmn2r73	UTSW	7	85858221	missense	probably benign	0.00
R6111:Vmn2r73	UTSW	7	85871789	missense	probably benign
R6233:Vmn2r73	UTSW	7	85869891	missense	probably benign	0.00
R6278:Vmn2r73	UTSW	7	85872932	missense	probably benign
R6283:Vmn2r73	UTSW	7	85871841	missense	probably benign	0.00
R6307:Vmn2r73	UTSW	7	85857620	missense	probably damaging	1.00
R6413:Vmn2r73	UTSW	7	85870336	nonsense	probably null
R6782:Vmn2r73	UTSW	7	85870355	missense	probably benign	0.00
R6884:Vmn2r73	UTSW	7	85858005	missense	probably benign	0.05
R6921:Vmn2r73	UTSW	7	85858238	missense	probably benign	0.27
R7169:Vmn2r73	UTSW	7	85858455	nonsense	probably null
R7206:Vmn2r73	UTSW	7	85872867	missense	probably benign	0.03
R7311:Vmn2r73	UTSW	7	85871984	missense	possibly damaging	0.80
R7650:Vmn2r73	UTSW	7	85871939	missense	probably benign
R7811:Vmn2r73	UTSW	7	85875748	missense	possibly damaging	0.77
R8263:Vmn2r73	UTSW	7	85858411	missense	probably benign
R8301:Vmn2r73	UTSW	7	85858302	missense	probably benign	0.00
R8341:Vmn2r73	UTSW	7	85857920	missense	probably benign	0.01
R8500:Vmn2r73	UTSW	7	85870430	missense	probably benign	0.01
R8673:Vmn2r73	UTSW	7	85872694	missense	probably benign	0.01
R8719:Vmn2r73	UTSW	7	85871887	missense	probably damaging	0.96
R8783:Vmn2r73	UTSW	7	85858460	missense	probably damaging	0.96
R8828:Vmn2r73	UTSW	7	85871971	missense	probably damaging	0.98
R8860:Vmn2r73	UTSW	7	85872941	splice site	probably benign
R9002:Vmn2r73	UTSW	7	85858076	missense	probably benign	0.32
R9008:Vmn2r73	UTSW	7	85872696	missense	probably damaging	0.96
R9159:Vmn2r73	UTSW	7	85872723	missense	possibly damaging	0.90
R9180:Vmn2r73	UTSW	7	85857915	missense	probably benign	0.07
R9198:Vmn2r73	UTSW	7	85869779	missense	probably benign	0.01
R9329:Vmn2r73	UTSW	7	85869865	missense	probably benign
R9448:Vmn2r73	UTSW	7	85872819	missense	probably benign	0.00
R9463:Vmn2r73	UTSW	7	85857587	missense
R9589:Vmn2r73	UTSW	7	85870451	missense	probably benign	0.03
X0023:Vmn2r73	UTSW	7	85858456	missense	probably benign	0.31
Z1176:Vmn2r73	UTSW	7	85871968	missense	probably damaging	0.99
Z1177:Vmn2r73	UTSW	7	85872272	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCAGCATCTCATGGAGTG -3'
(R):5'- AACTCGCCCATAAAGTTTGTCC -3'

Sequencing Primer
(F):5'- TGAGTGGGCTACAGCATATATAGC -3'
(R):5'- CCTATTTTTGTGGAAATCTCAAGGC -3'

Posted On

2015-12-29