Mutagenetix > Incidental Mutation

Incidental Mutation 'R3732:Lag3'

368244

Institutional Source

Beutler Lab

Gene Symbol

Lag3

Ensembl Gene

ENSMUSG00000030124

Gene Name

lymphocyte-activation gene 3

Synonyms

CD223, Ly66, LAG-3

MMRRC Submission

040720-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R3732 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

124904361-124911705 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 124910140 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 155 (S155T)

Ref Sequence

ENSEMBL: ENSMUSP00000032217 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032216] [ENSMUST00000032217]

AlphaFold

Q61790

Predicted Effect

probably benign
Transcript: ENSMUST00000032216

SMART Domains

Protein: ENSMUSP00000032216
Gene: ENSMUSG00000030122

Domain	Start	End	E-Value	Type
Pfam:Prothymosin	2	98	1e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000032217
AA Change: S155T

PolyPhen 2 Score 0.051 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000032217
Gene: ENSMUSG00000030124
AA Change: S155T

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IG	29	163	1.3e-2	SMART
IG	170	254	6.51e-3	SMART
IG	261	345	4.96e-8	SMART
Blast:IG_like	348	421	2e-28	BLAST
transmembrane domain	443	465	N/A	INTRINSIC
low complexity region	492	521	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134916

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139571

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181150

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203422

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000204671

Meta Mutation Damage Score

0.0987

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.6%
20x: 93.6%

Validation Efficiency

93% (56/60)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this gene have a generally normal phenotype but do display reduced natural killer cell activity and increased T cell response to infection. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006E09Rik	C	T	11: 101,988,452	Q17*	probably null	Het
1700012B07Rik	G	T	11: 109,794,154	C172*	probably null	Het
Aacs	C	T	5: 125,506,262	T294M	probably damaging	Het
Ablim1	A	T	19: 57,049,460		probably null	Het
Adgrf4	C	T	17: 42,672,581	G70E	probably damaging	Het
Adgrl3	C	A	5: 81,794,946	H1474Q	probably benign	Het
Adgrv1	T	C	13: 81,556,956	I1578M	probably damaging	Het
Afg3l1	G	A	8: 123,501,233	G547D	probably damaging	Het
Ambra1	G	A	2: 91,810,131	R635H	probably damaging	Het
Araf	TACACACACACACACACA	TACACACACACACACA	X: 20,850,226		probably benign	Het
Arhgef10l	AGAGGAGGAGGAGGAGGA	AGAGGAGGAGGAGGA	4: 140,581,619		probably benign	Het
Bcl7b	A	G	5: 135,180,913	T141A	probably benign	Het
Calm5	T	A	13: 3,854,337	N10K	probably damaging	Het
Camsap1	T	C	2: 25,938,344	R1123G	probably damaging	Het
Chrna3	T	C	9: 55,015,894	K210R	probably benign	Het
Ciz1	A	G	2: 32,367,483	N180S	possibly damaging	Het
Cntnap3	G	A	13: 64,740,999	A1162V	possibly damaging	Het
Cox5b	C	T	1: 36,693,260	P114L	probably damaging	Het
Cpsf2	T	C	12: 101,987,308	I199T	probably damaging	Het
Cyp2a4	A	G	7: 26,312,827	D345G	probably damaging	Het
Cyp2s1	C	T	7: 25,803,954	R424Q	probably null	Het
D1Pas1	C	A	1: 186,968,097	S74R	probably benign	Het
Ddx4	T	A	13: 112,611,982	I487F	possibly damaging	Het
Dnah5	A	T	15: 28,409,122	E3562V	possibly damaging	Het
Dpf3	T	C	12: 83,269,507	D330G	possibly damaging	Het
Edem1	T	C	6: 108,841,621	F197L	probably damaging	Het
Ergic2	T	C	6: 148,202,522	D79G	probably damaging	Het
Espl1	A	G	15: 102,312,989	I944V	probably damaging	Het
Fam102a	G	A	2: 32,566,292	S322N	probably benign	Het
Fam111a	T	C	19: 12,587,550	L221P	possibly damaging	Het
Fat1	T	C	8: 44,953,269	V1019A	possibly damaging	Het
Fbxl21	T	A	13: 56,527,017	H60Q	probably benign	Het
Fbxw7	A	C	3: 84,925,707	K19Q	possibly damaging	Het
Gldn	A	G	9: 54,338,662	K499R	possibly damaging	Het
Gm5514	T	G	19: 21,938,252		noncoding transcript	Het
Gria4	C	T	9: 4,513,295	M271I	probably benign	Het
Herc1	C	T	9: 66,445,640	T2136I	probably damaging	Het
Igsf10	A	G	3: 59,325,714	F1866S	probably benign	Het
Iqcg	G	T	16: 33,053,626		probably benign	Het
Itih2	A	T	2: 10,105,670	F537I	probably benign	Het
Itpr2	T	C	6: 146,382,700	D533G	probably damaging	Het
Kcnk15	A	G	2: 163,853,813	K22E	probably benign	Het
Lars	T	C	18: 42,212,602	E1003G	probably benign	Het
Layn	T	A	9: 51,059,544	N233I	probably damaging	Het
Lgi1	T	C	19: 38,306,246	Y465H	probably damaging	Het
Lrig1	C	T	6: 94,611,576	A531T	possibly damaging	Het
Lrrtm4	A	G	6: 80,019,655		probably benign	Het
Lsamp	T	A	16: 42,144,572	L264H	probably damaging	Het
Mthfd2	T	C	6: 83,313,475	E39G	probably damaging	Het
Mtx2	C	A	2: 74,847,262	A22E	probably damaging	Het
Nipal3	T	C	4: 135,463,846	T325A	probably damaging	Het
Nlrp14	A	T	7: 107,182,367	Y257F	probably benign	Het
Ola1	G	C	2: 73,156,860	R143G	probably damaging	Het
Olfr301	T	G	7: 86,412,633	I90M	probably damaging	Het
Otog	A	G	7: 46,288,368	T1834A	probably benign	Het
Oxr1	T	C	15: 41,848,701	I656T	probably damaging	Het
Panx1	GTTCTTCT	GTTCT	9: 15,006,171		probably benign	Het
Pcdh18	T	C	3: 49,754,791	S692G	probably benign	Het
Pcdhga1	A	G	18: 37,664,123	T727A	probably benign	Het
Pde9a	A	G	17: 31,448,427	E3G	possibly damaging	Het
Prl8a1	T	C	13: 27,579,733	E37G	probably damaging	Het
Rlf	C	T	4: 121,148,324	G1153D	probably benign	Het
Robo2	A	C	16: 73,920,747	L1159W	possibly damaging	Het
Sfxn5	T	C	6: 85,299,276		probably benign	Het
Siah2	A	G	3: 58,676,250	V205A	probably damaging	Het
Spindoc	A	C	19: 7,374,301	L202R	probably damaging	Het
Spock3	T	A	8: 63,345,699	D251E	probably damaging	Het
Srp68	T	A	11: 116,273,956	K51*	probably null	Het
Ssbp2	T	C	13: 91,524,607	Y29H	probably damaging	Het
Sspo	T	C	6: 48,449,930	V231A	probably damaging	Het
Stk4	T	A	2: 164,088,908	M143K	probably benign	Het
Tecta	C	T	9: 42,392,106	V77M	possibly damaging	Het
Trpc3	A	T	3: 36,638,559	D761E	probably benign	Het
Tubb2a	T	C	13: 34,075,264	E181G	probably damaging	Het
Ugcg	C	T	4: 59,207,798	P46S	probably benign	Het
Ush2a	C	A	1: 188,944,760	N4756K	probably benign	Het
Vmn2r-ps159	G	T	4: 156,334,397		noncoding transcript	Het
Wapl	G	A	14: 34,736,764	V928I	probably damaging	Het
Zfand3	A	G	17: 30,192,656	K130R	probably benign	Het
Zfp934	A	T	13: 62,517,785	H347Q	probably damaging	Het

Other mutations in Lag3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01128:Lag3	APN	6	124909417	missense	probably damaging	1.00
IGL01867:Lag3	APN	6	124910906	missense	probably benign
IGL02880:Lag3	APN	6	124905471	missense	probably benign	0.01
R1502:Lag3	UTSW	6	124909243	missense	probably damaging	1.00
R1573:Lag3	UTSW	6	124909247	missense	possibly damaging	0.65
R1886:Lag3	UTSW	6	124909439	missense	probably damaging	0.99
R1907:Lag3	UTSW	6	124909487	missense	possibly damaging	0.52
R2508:Lag3	UTSW	6	124911309	missense	possibly damaging	0.91
R3016:Lag3	UTSW	6	124908466	missense	probably damaging	1.00
R3732:Lag3	UTSW	6	124910140	missense	probably benign	0.05
R3733:Lag3	UTSW	6	124910140	missense	probably benign	0.05
R3734:Lag3	UTSW	6	124910140	missense	probably benign	0.05
R4679:Lag3	UTSW	6	124904545	missense	possibly damaging	0.92
R4994:Lag3	UTSW	6	124904453	missense	unknown
R5057:Lag3	UTSW	6	124905355	missense	possibly damaging	0.58
R5527:Lag3	UTSW	6	124908629	missense	probably damaging	0.99
R7227:Lag3	UTSW	6	124908494	missense	possibly damaging	0.79
R7255:Lag3	UTSW	6	124910235	missense	probably benign	0.04
R8081:Lag3	UTSW	6	124905447	nonsense	probably null
R8138:Lag3	UTSW	6	124905492	missense	probably damaging	0.99
R8710:Lag3	UTSW	6	124908445	missense	probably damaging	1.00
R9126:Lag3	UTSW	6	124904846	missense	probably damaging	0.99
X0009:Lag3	UTSW	6	124905352	missense	possibly damaging	0.62

Predicted Primers

PCR Primer
(F):5'- TAAAGGCGAGCGTTTCAGGG -3'
(R):5'- AGTGCATCTTCTTCGTGGCC -3'

Sequencing Primer
(F):5'- GTTTCAGGGGGCACTTTCCAAC -3'
(R):5'- TCGCTACACGGTGCTGAG -3'

Posted On

2016-01-11