Mutagenetix > Incidental Mutation

Incidental Mutation 'R4797:Stk10'

369152

Institutional Source

Beutler Lab

Gene Symbol

Stk10

Ensembl Gene

ENSMUSG00000020272

Gene Name

serine/threonine kinase 10

Synonyms

Lok, Gek1

MMRRC Submission

042421-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.107) question?

Stock #

R4797 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

32483305-32574587 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 32548471 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 346 (N346K)

Ref Sequence

ENSEMBL: ENSMUSP00000099885 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102821]

AlphaFold

O55098

Predicted Effect

probably benign
Transcript: ENSMUST00000102821
AA Change: N346K

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000099885
Gene: ENSMUSG00000020272
AA Change: N346K

Domain	Start	End	E-Value	Type
S_TKc	36	294	8.66e-92	SMART
low complexity region	316	334	N/A	INTRINSIC
low complexity region	544	577	N/A	INTRINSIC
Pfam:PKK	586	724	1.9e-41	PFAM
Pfam:PKK	754	894	2.2e-37	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143397

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.8%

Validation Efficiency

96% (69/72)

MGI Phenotype

FUNCTION: This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. Mice deficient for this gene product are viable, but exhibit altered integrin-mediated lymphocyte adhesion characteristics. The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. [provided by RefSeq, Jul 2008]
PHENOTYPE: Targeted disruption of this gene results in enhanced cell adhesion in mitogen-stimulated T cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca9	T	C	11: 110,008,945 (GRCm39)	T1195A	probably benign	Het
Apobr	G	A	7: 126,186,756 (GRCm39)	E756K	probably benign	Het
Arpc3	A	G	5: 122,542,215 (GRCm39)	E77G	possibly damaging	Het
Atp2b2	A	T	6: 113,766,847 (GRCm39)	M464K	possibly damaging	Het
Atxn7l2	A	G	3: 108,111,866 (GRCm39)	S379P	probably damaging	Het
Ccdc91	A	T	6: 147,493,641 (GRCm39)	E344D	unknown	Het
Cdc42bpg	G	A	19: 6,370,477 (GRCm39)	R1190Q	probably damaging	Het
Cdh17	C	A	4: 11,810,390 (GRCm39)	Q694K	probably benign	Het
Chordc1	G	T	9: 18,203,672 (GRCm39)		probably benign	Het
Copg1	A	G	6: 87,880,450 (GRCm39)		probably benign	Het
Dcbld1	T	C	10: 52,160,223 (GRCm39)	V37A	probably damaging	Het
Ddb2	A	G	2: 91,067,163 (GRCm39)		probably benign	Het
Dok5	A	T	2: 170,672,042 (GRCm39)	R115*	probably null	Het
Drc7	T	C	8: 95,800,925 (GRCm39)	I649T	probably damaging	Het
Efr3a	A	G	15: 65,729,437 (GRCm39)	T713A	probably damaging	Het
Epg5	G	A	18: 78,073,614 (GRCm39)	D2494N	probably benign	Het
Eps15	G	A	4: 109,223,727 (GRCm39)		probably benign	Het
Glb1l3	T	C	9: 26,739,742 (GRCm39)	D356G	probably damaging	Het
Gm1818	A	T	12: 48,602,393 (GRCm39)		noncoding transcript	Het
Gsta5	A	T	9: 78,211,679 (GRCm39)	Y147F	probably benign	Het
Hcrtr2	C	A	9: 76,161,816 (GRCm39)	M191I	probably damaging	Het
Heatr1	G	A	13: 12,426,929 (GRCm39)	E685K	probably benign	Het
Hsd3b2	A	T	3: 98,618,979 (GRCm39)	L322Q	probably damaging	Het
Hsd3b9	T	A	3: 98,363,747 (GRCm39)	R62*	probably null	Het
Htra4	T	C	8: 25,523,675 (GRCm39)	T297A	probably damaging	Het
Il22	C	A	10: 118,041,058 (GRCm39)	R55S	probably damaging	Het
Ints1	G	A	5: 139,757,631 (GRCm39)	T324M	possibly damaging	Het
Ints15	A	G	5: 143,297,504 (GRCm39)	F181S	probably benign	Het
Ints7	T	C	1: 191,329,045 (GRCm39)	V268A	probably damaging	Het
Kctd20	G	A	17: 29,185,766 (GRCm39)	V370I	probably damaging	Het
Lama1	T	A	17: 68,023,770 (GRCm39)	M55K	probably benign	Het
Larp1	C	A	11: 57,938,806 (GRCm39)	S494*	probably null	Het
Ldb3	A	T	14: 34,277,470 (GRCm39)	H262Q	possibly damaging	Het
Lepr	C	A	4: 101,637,244 (GRCm39)	T711K	possibly damaging	Het
Mon2	T	C	10: 122,852,422 (GRCm39)	I984V	probably benign	Het
Naip2	T	A	13: 100,298,243 (GRCm39)	S598C	probably damaging	Het
Oasl1	A	G	5: 115,066,217 (GRCm39)	M112V	probably benign	Het
Or2d2b	A	T	7: 106,705,234 (GRCm39)	M278K	probably benign	Het
Or6c214	A	T	10: 129,590,390 (GRCm39)	S310T	probably benign	Het
Or7c19	G	T	8: 85,957,567 (GRCm39)	A148S	probably benign	Het
P2ry1	T	A	3: 60,910,881 (GRCm39)	S7T	probably benign	Het
Pidd1	G	T	7: 141,022,899 (GRCm39)	R98S	possibly damaging	Het
Pkd1l1	A	C	11: 8,911,340 (GRCm39)	F312L	unknown	Het
Pla2r1	A	T	2: 60,334,524 (GRCm39)	M416K	possibly damaging	Het
Pold1	T	C	7: 44,191,325 (GRCm39)	E194G	possibly damaging	Het
Poldip2	T	A	11: 78,404,813 (GRCm39)	Y77N	probably damaging	Het
Ppp2r3d	G	T	9: 101,089,179 (GRCm39)	N381K	probably benign	Het
Prrc2a	G	A	17: 35,369,018 (GRCm39)	P2006L	probably damaging	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Rsu1	A	G	2: 13,221,537 (GRCm39)		probably benign	Het
Selenoi	T	A	5: 30,457,740 (GRCm39)	W90R	probably damaging	Het
Spag17	T	A	3: 99,891,795 (GRCm39)	D216E	possibly damaging	Het
Spata31	C	A	13: 65,070,556 (GRCm39)	Y901*	probably null	Het
Ssrp1	T	A	2: 84,876,066 (GRCm39)	Y607*	probably null	Het
Surf1	G	T	2: 26,806,358 (GRCm39)		probably benign	Het
Synj2	A	T	17: 6,084,163 (GRCm39)	E283V	probably damaging	Het
Tg	G	A	15: 66,629,855 (GRCm39)		probably null	Het
Traf1	A	T	2: 34,846,289 (GRCm39)	D42E	probably benign	Het
Ttn	T	C	2: 76,571,209 (GRCm39)	I26561M	probably damaging	Het
Ubp1	T	C	9: 113,785,070 (GRCm39)	Y128H	probably damaging	Het
Vmn1r216	T	A	13: 23,283,506 (GRCm39)	I63K	probably benign	Het
Vmn1r49	T	A	6: 90,049,612 (GRCm39)	H130L	probably benign	Het
Vmn2r90	C	T	17: 17,932,567 (GRCm39)	T158I	probably damaging	Het
Vps13d	T	C	4: 144,780,725 (GRCm39)	S885G	probably damaging	Het

Other mutations in Stk10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01105:Stk10	APN	11	32,527,740 (GRCm39)	missense	probably benign	0.33
IGL01285:Stk10	APN	11	32,560,653 (GRCm39)	missense	possibly damaging	0.91
IGL01983:Stk10	APN	11	32,539,460 (GRCm39)	missense	probably benign	0.05
IGL03177:Stk10	APN	11	32,564,592 (GRCm39)	missense	probably damaging	1.00
IGL03183:Stk10	APN	11	32,554,143 (GRCm39)	missense	possibly damaging	0.50
coquet	UTSW	11	32,527,764 (GRCm39)	missense
legacy	UTSW	11	32,554,166 (GRCm39)	nonsense	probably null
mignon	UTSW	11	32,537,363 (GRCm39)	missense	probably damaging	1.00
R0481_stk10_383	UTSW	11	32,564,708 (GRCm39)	missense	probably damaging	1.00
FR4976:Stk10	UTSW	11	32,564,520 (GRCm39)	critical splice acceptor site	probably benign
R0003:Stk10	UTSW	11	32,539,460 (GRCm39)	missense	probably benign	0.05
R0008:Stk10	UTSW	11	32,537,305 (GRCm39)	splice site	probably benign
R0056:Stk10	UTSW	11	32,567,851 (GRCm39)	missense	possibly damaging	0.95
R0076:Stk10	UTSW	11	32,553,722 (GRCm39)	missense	probably benign
R0227:Stk10	UTSW	11	32,567,859 (GRCm39)	missense	probably damaging	1.00
R0440:Stk10	UTSW	11	32,554,190 (GRCm39)	missense	probably damaging	1.00
R0454:Stk10	UTSW	11	32,546,724 (GRCm39)	missense	probably damaging	0.99
R0481:Stk10	UTSW	11	32,564,708 (GRCm39)	missense	probably damaging	1.00
R0504:Stk10	UTSW	11	32,567,882 (GRCm39)	missense	probably benign	0.04
R0790:Stk10	UTSW	11	32,548,653 (GRCm39)	missense	probably benign	0.00
R1439:Stk10	UTSW	11	32,567,919 (GRCm39)	missense	probably damaging	0.98
R1539:Stk10	UTSW	11	32,483,440 (GRCm39)	missense	possibly damaging	0.85
R1770:Stk10	UTSW	11	32,572,464 (GRCm39)	missense	possibly damaging	0.94
R4304:Stk10	UTSW	11	32,560,634 (GRCm39)	missense	probably damaging	0.97
R4430:Stk10	UTSW	11	32,483,552 (GRCm39)	missense	possibly damaging	0.81
R4702:Stk10	UTSW	11	32,505,172 (GRCm39)	missense	probably benign	0.28
R5447:Stk10	UTSW	11	32,554,166 (GRCm39)	nonsense	probably null
R5801:Stk10	UTSW	11	32,546,748 (GRCm39)	missense	probably benign	0.01
R5802:Stk10	UTSW	11	32,546,748 (GRCm39)	missense	probably benign	0.01
R6129:Stk10	UTSW	11	32,565,871 (GRCm39)	missense	probably damaging	1.00
R6154:Stk10	UTSW	11	32,553,654 (GRCm39)	splice site	probably null
R6175:Stk10	UTSW	11	32,553,761 (GRCm39)	missense	possibly damaging	0.46
R6185:Stk10	UTSW	11	32,527,749 (GRCm39)	missense	probably benign	0.13
R6520:Stk10	UTSW	11	32,538,839 (GRCm39)	missense	probably damaging	1.00
R6824:Stk10	UTSW	11	32,537,363 (GRCm39)	missense	probably damaging	1.00
R7259:Stk10	UTSW	11	32,548,497 (GRCm39)	missense	probably benign	0.00
R7649:Stk10	UTSW	11	32,527,764 (GRCm39)	missense
R8331:Stk10	UTSW	11	32,538,928 (GRCm39)	missense
R8847:Stk10	UTSW	11	32,539,427 (GRCm39)	missense
R9252:Stk10	UTSW	11	32,538,915 (GRCm39)	missense
R9367:Stk10	UTSW	11	32,538,878 (GRCm39)	missense
X0027:Stk10	UTSW	11	32,537,361 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGGTAGCCTGTTTAAGAAAGGC -3'
(R):5'- CTGAATTCTGGCATCCATGGAC -3'

Sequencing Primer
(F):5'- AGCCTGTTTAAGAAAGGCTGTGG -3'
(R):5'- GGAACAGGCACCTTTAAGTCATTGTC -3'

Posted On

2016-02-04